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Kochueva M.V.,Nizhny Novgorod Regional Oncological Hospital | Ignatieva N.Y.,Moscow State University | Zakharkina O.L.,Russian Academy of Sciences | Kamensky V.A.,RAS Institute of Applied Physics | And 3 more authors.
Sovremennye Tehnologii v Medicine

The aim of the investigation is complex study of the mechanisms of radiation-induced collagen damage and following recovery of its structure on various levels of multilayer organization. Materials and Methods. Rat tail tendons were used as the model of collagen containing tissue. The animals were exposed to radiation at the dosage of 2, 4, 6, 8, and 10 Gy using gamma-apparatus "Luch-1" (Russia) in vivo. The collagen structure was studied 1 and 7 days after the radiation by trypsin-resistance test, differential scanning calorimetry, confocal microscopy with second-harmonic generation imaging, and cross polarization optical coherence tomography. Results. There were determined certain regularities of collagen structural changes depending on radiation time. One day after the radiation of collagen containing tissue the leading processes were those of collagen structural damage at molecular level due to reactive oxygen species exposure. Within a week after the radiation, the collagen structure recovered partially, and therefore, there recovered its resistance to proteolytic activity. Ionizing radiation initiated the cascade of reactions beginning with direct and indirect protein damage, and resulting in its remodeling as a result of nonenzymic bridging between triple helix forming quaternary structure of collagen. Source

Kleshnin M.S.,RAS Institute of Applied Physics | Fiks I.I.,RAS Institute of Applied Physics | Plekhanov V.I.,RAS Institute of Applied Physics | Gamayunov S.V.,Nizhny Novgorod Regional Oncological Hospital | Turchin I.V.,RAS Institute of Applied Physics
Laser Physics Letters

Photodynamic therapy is one of the most promising methods for the treatment of oncological, inflammatory and degenerative diseases of the skin. This technique is based on light irradiation of a photosensitizer that has been injected into the patient's body prior to the procedure, with determination of the efficacy of treatment requiring proper assessment of the drug concentration in the tissue lesion and the extent of sensitizer photobleaching during irradiation. We have developed a compact and low cost device based on a fluorescence imaging for localizing the tumor in the patient's body, tracking the position of the tissue lesion during involuntary movements of the patient, estimating accumulation of the sensitizer in the tumor relative to the surrounding tissues and monitoring photobleaching of the sensitizer during laser irradiation. The system that has been created is compatible with any therapeutic laser and includes a single CCD camera and two LEDs, one in the excitation band and the other in the emission band of the 'Photoditazin' sensitizer. In this letter we also present the test results of the device in model experiments and in preliminary clinical trials. The results obtained clearly show the efficacy of the system for monitoring sensitizer photobleaching during photodynamic therapy. © 2015 Astro Ltd. Source

Stcherbatyuk T.G.,Nizhny Novgorod State Medical Academy | Davydenko D.V.,Nizhny Novgorod State Medical Academy | Novikova V.A.,Nizhny Novgorod Regional Oncological Hospital
Sovremennye Tehnologii v Medicine

The aim of the investigation was to assess the role of ecological problems and oxidative homeostasis parameters in life expectancy prognosis of oncology patients in the territories with different anthropogenic load level. Materials and Methods. A group of oncology patients included 80 males with oropharyngeal tumors (III-IV stage tumors of oral and oropharyngeal cavity), mean age being 58.63±0.68. According to clinical and morphological characteristics of the disease, and social and adaptation criteria all the patients were referred to a homogeneous group. A control group consisted of 10 men from different territories with no oncology in past history. Results. Pro-, antioxidant system components were found to be the markers of latent inhomogeneity in a group of patient with III-IV stage oropharyngeal tumors. Oxidative stress level in blood of patients with similar clinicopathologic and anatomical characteristics appeared to be different. Regression analysis findings showed the life expectancy in patients with III-IV stage oropharyngeal tumors to be associated with anthropogenic load index of residence place, red blood cell superoxide dismutase activity, maximum chemiluminescence intensity, TNF-α concentration, ketondinitrophenylhydrazine level in induced oxidation and malondialdehyde concentration in blood plasma. Conclusion. The study has demonstrated for the first time the relationship of environmental factor and oxidative homeostasis parameters, and their integrated effect on life expectancy of oncology patients. Source

Malinovskaya S.L.,Nizhny Novgorod State Medical Academy | Ermolayev V.S.,Nizhny Novgorod Regional Oncological Hospital | Bavrina A.P.,Nizhny Novgorod State Medical Academy | Monich V.A.,Nizhny Novgorod State Medical Academy
Sovremennye Tehnologii v Medicine

The aim of the investigation was to assess the level of protein oxidative modification (POM) and lipid peroxidation (LP) products in muscular tissue of rats with radiation disease when exposed to low-intensity red light. Materials and Methods. We studied the level of direct and induced POM, as well as LP parameters in rat femoral muscular tissue after ionizing radiation exposure and correction of radiation sequellae by low-intensity red light. Free-radical oxidation intensity was estimated by the content of POL products - neutral and basic aliphatic aldehyde- and cetone-dinitrophenylhydrazones; the level of LP products - diene conjugates, triene conjugates and Schiff's bases. SPSS software application package was used for statistical processing. Results. Rat muscular tissue showed the decreased intensity of oxidative processes - POM and LP after the ionizing radiation area had been exposed to low-intensity incoherent red light, i.e., there was the intensification of oxidative processes. The following exposure of the same area to low-intensity red light resulted in decreased level of oxidation products. Conclusion. The exposure of biological tissues to low-intensity incoherent red light after ionizing radiation contributes to the decrease of accumulation of POM and LP products and prevents from ozidative stress development. Source

Bazanov K.V.,Nizhny Novgorod Regional Oncological Hospital | Kuznetsov S.S.,Nizhny Novgorod Regional Oncological Hospital | Terentiev I.G.,Nizhny Novgorod State Medical Academy
Sovremennye Tehnologii v Medicine

One of the major problems in drug therapy administration in cancer patients is the lack of information on sensitivity of tumor cells to chemotherapeutic agents (initial uncertainty). A dividing cancer cell is known to be the primary point of chemotherapeutic agent application. The authors have developed a mathematical model, where tumor growth is considered as a complex balance between cancer cell division and death. There have been studied the combinations of parameters of proliferative activity of cells and cell death, when it is possible to reach maximum effect in cytostatic treatment. The efficacy of adjuvant chemotherapy in the prevention of colorectal cancer recurrences and metastases has been analyzed. © 2014, Nizhny Novgorod State Medical Academy. All rights reserved. Source

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