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Levin G.Ya.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Sukhareva E.G.,Nizhny Novgorod Institute of Traumatology and Orthopedics
Gematologiya i Transfusiologiya | Year: 2015

The effects of erythrocyte preservation on their electrophoretic mobility (EPM) and morphology were studied. Erythrocytes were isolated from the blood, collected In CPDA-1 preserving agent, In 30 donors. Electrophoretic mobility was studied under a microscope in a special box with Ag/AgCI electrodes.The morphology of erythrocytes was studied under a Primo Star microscope (Carl Zeiss, Germany). The microvesicles (MV) were isolated from erythrocyte suspension by ultracentrifugation at 100 000g.The erythrocyte EPM reduced significantly after preservation. One of the causes of this reduction was MV formation, caused, among other things, by disk-spheric transformation of erythrocytes - marked anisocytosis and poikilocytosis. These processes were more rapid in pre-washed erythrocytes, despite their storage in the same preserving solution. More intense and rapid morphological changes in erythrocytes were associated with greater reduction of their EPM and formation of MV.


Sheremet'ev Y.A.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Popovicheva A.N.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Egorihina M.N.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Levin G.Y.,Nizhny Novgorod Institute of Traumatology and Orthopedics
Biophysics (Russian Federation) | Year: 2013

The relationship between shape and spontaneous and fibrinogen-induced aggregation change in human erythrocytes was studied. Spontaneous and fibrinogen-induced erythrocyte aggregation was investigated using a rheoscope designed according to the method of H. Schmid-Schonbein et al. (1973). The erythrocyte shape was studied by means of light microscopy. It was shown that plasma enriched with lysophosphatidic acid and ATP depletion of erythrocytes led to the change of erythrocyte shape: discocytes transformed into echinocytes. It was found that spontaneous aggregation of such cells was considerably decreased. Aggregation of erythrocytes, treated with lysophosphatidic acid, was diminished more markedly. Fibrinogen-induced aggregation of echinocytes, obtained after treatment with lysophosphatidic acid and produced by ATP depletion, was also greatly reduced. © 2013 Pleiades Publishing, Ltd.


Peretyagin S.P.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Martusevich A.K.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Solovyeva A.G.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Zimin Y.V.,Nizhny Novgorod Institute of Traumatology and Orthopedics | Peretyagin P.V.,Nizhny Novgorod Institute of Traumatology and Orthopedics
Bulletin of Experimental Biology and Medicine | Year: 2013

Hepatotoxicity of ozone in total systems treatment was evaluated by the functioning of hepatic oxidoreductases. Activities of lactate dehydrogenase and alcohol dehydrogenase were measured in liver homogenates of Wistar rats, injected daily with saline with saturating ozone concentrations of 3000, 10,000, and 40,000 μg/liter or placebo for 30 days. Systemic ozone treatment had a two-step effect on the hepatic oxidoreductases. Low doses (0.6 μg) promoted a moderate physiological stimulation of the enzymes, while in doses >2 μg ozone led to progressive tissue hypoxia and accumulation of toxic products in the liver. © 2013 Springer Science+Business Media New York.

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