Nizam Institute of Pharmacy and Research Center

Hyderabad, India

Nizam Institute of Pharmacy and Research Center

Hyderabad, India
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Stability representing Ultra Performance LC method was developed for Assay of multi drug Combination of Rilpivirine, Emtricitabine and Tenofovir alafenamide in bulk active pharmaceutical ingredients & its tablet formulation, Validation was performed for all parameters. Retention times of Reference Standard Emtricitabine, Tenofovir Alafenamide and Rilpivirine was found to be 0.965, 1.528, 2.186 respectively, with the flow rate of 0.3 mille liters per minute by Injecting Volume of 2 micro liters by maintaining Run time of 3 minutes. Developed method was subjected to forced degradation studies under specified conditions, which meets the required criteria. Degradation product at 1.975 Rt was collected under various stress condition, cleavage of imidazole ring of tenafovir alafenamide confirmed with Proton NMR, ESI -MASS in + MODE. © 2017, Oriental Scientific Publishing Company. All rights reserved.


Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Rao A.S.,Bhaskar Pharmacy College | Thayyil H.A.,Nizam Institute of Pharmacy and Research Center | Ahemad R.S.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Pharmacognosy Journal | Year: 2010

The present study was carried out to evaluate the anti-ulcer activity of cassia auriculata leaf extract against pylorous ligation induced gastric ulcer. The methanolic leaf extract of cassia auriculata at dose of 300 mg/kg p.o. markedly decrease the incidence of ulcers in pyloric ligatied rats. In pyloric ligated rats, there was an increase in the gastric volume, free and total acidity and ulcerative index as compared to the control group. The methanolic leaf extract of cassia auriculata at dose of, 300 mg/kg showed significant reduction in the above parameters which was comparable to the standard drug famotidine(10 mg/kg). Cassia auriculata extract showed protection index 79.4 %, whereas standard drug famotidine showed protection index 90.7%.


Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Srinivasa Rao A.,Bhaskar Pharmacy College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The present study was carried out to estimate the phenolics compound, Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata. by high-performance liquid chromatography (HPLC). Methods: For the estimation of Rutin, Quercetin and Kaempferol by HPLC method, the mobile phase used are, Acetonitrile and Phosphate buffer (pH=5.8) in ratio of 55: 45.Quantification of Rutin, Quercetin and Kaempferol was carried by Athena C18 column and absorbance was measured at 254 nm with flow rate of 1 ml/min. Results: In HPLC analysis the retention time(Rt) of standards, Rutin, Quercetin and Kaempferol were found to be 2.357, 6.093 and 9.373 respectively, while the Retention times of Rutin and Kaempferol in Brassica oleracea L.var capitata are 2.387, 6.060 and 9.113 which are found to be matching with standards retention time values respectively. Conclusion: Thus this HPLC method was found to be simple and rapid for quantitative determination of Rutin, Quercetin and Kaempferol in Brassica oleracea L.var capitata.


Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Rao A.S.,Bhaskar Pharmacy College | Thayyil H.,Nizam Institute of Pharmacy and Research Center | Ahemad S.R.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Pharmacognosy Journal | Year: 2011

The hepataprotective activity of the methanolic leaf extract of Melia azedarach was investigated against paracetamol induced hepatic damage in rats. Over dosing of paracetamol to rats is reported to decrease the activity of antioxidative enzymes (GPx, GST, SOD and CAT ) in liver and increase the serum enzymes (SGOT , SGPT and Alkaline phosphate), bilirubin and decrease the total proteins content. Melia azedarach leaf extract maintained the activity of antioxidant enzymes and as well as serum enzymes to the normal level. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.


Thayyil A.H.,Nizam Institute of Pharmacy and Research Center | Kottai Muthu A.,Annamalai University
Der Pharmacia Lettre | Year: 2015

The study was designed to examine the invitro antioxidant activities of various extracts of aerial parts of Chomelia asiatica. The antioxidant activity was evaluated by DPPH (α,α-diphenyl-β-picrylhydrazyl) radical scavenging activity, Super Oxide Anion Scavenging Activity with reference standard Rutin, Quercetin respectively and estimate the amount of total phenol. The ethyl acetate extract of Chomelia asiatica was found to more effective in the DPPH radical scavenging activity. The IC50 of the ethyl acetate extract of Chomelia asiatica and Rutin were found to be 460μg/ml and 480μg/ml respectively. An IC50 value was found that ethyl acetate extract of Chomelia asiatica is more effective in scavenging superoxide radical than that of methanol and petroleum ether extract. But when compare to the all the three extracts with Quercetin (standard), the ethyl acetate extract of the Chomelia asiatica showed the similar result. In addition, the ethyl acetate extract of Chomelia asiatica was found to contain a noticeable amount of total phenols, which play a major role in controlling antioxidants. It is concluded that a aerial parts of ethyl acetate extract of Chomelia asiatica, which contains large amounts of phenolic compounds, exhibits high antioxidant and free radical scavenging activities. These in vitro assays indicate that this plant extracts is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.


Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Srinivasa Rao A.,Bhaskar Pharmacy College | Ahemad S.R.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

The aim of this study was to investigate the antioxidant activities and phytochemical analysis of Brassica oleracea L. var. capitata. The phytochemical screening was carried on the leaves extracts of Brassica oleracea L. var. capitata revealed the presence of some active ingredients such as Alkaloids, Tannins, Saponins, Phenols, Glycosides, Steroids, Terpenoids and Flavonoids. The ethanol leaves extract were also evaluated for their total phenolic contents and antioxidant activity using DPPH radical scavenging assay and reducing power assay. The result of the present study showed that the ethanolic leaves extract of Brassica oleracea L. var. capitata which contains highest amount of phenolic compounds exhibited the greatest anti-oxidant activity than Petroleum Ether, ethylacetate, chloroform and aqueous extracts. The high scavenging property of may be due to hydroxyl groups existing in the phenolic compounds.


Sattar M.A.,Pacific University at Udaipur | Iqbal M.M.,Nizam Institute of Pharmacy and Research Center | Syed Asad B.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

Hepatic and Renal systems are involved in the metabolism and excretion of various xenobiotics, environmental pollutants etc. Hence they are highly prone to attack by oxidative stress and generation of excessive concentration of free radicals. This results in tissue necrosis and damage of these organ systems. Keeping in view of its antioxidant and organ protective activities, the present research work is made by use of Albino Rats (Wister) to assess the influence of pre-treatment with methanolic extract of Phyllanthus amarus seeds and ethanolic extract of Leucas aspera leaves in simvastatin induced hepatotoxicity, where as in cisplatin induced nephrotoxicity. Liver and Kidney functions were assessed by collecting the blood samples from each group & evaluating the biochemical parameters. Histopathological studies were done by isolating the liver and kidney of all the groups. Treatment with methanolic and ethanolic extracts has brought back the elevated levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), total & direct bilirubin, serum cholesterol and serum triglycerides in simvastatin induced hepatotoxic rats to near normal levels. Whereas methanolic extract, ethanolic extract and aqueous extract prevented the reduction in body weight and reduced the levels of total urea and serum creatinine in cisplatin induced nephrotoxicity. Histopathological observation exhibited the improved hepatic and renal anatomy that is reversed the damage. Thus methanolic extract of Phyllanthus amarus seeds and ethanolic extract of Leucas aspera leaves possess hepatoprotective and nephroprotective activities, comparatively Leucas aspera leaves shows better results, this may be due to presence of phenolic compounds like flavonoids steroids and tannins known to possess antioxidant activity.


Sri B.U.,Sri Padmavathi Mahila University | Kiranmai M.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center | Muzib Y.I.,Sri Padmavathi Mahila University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

Self-emulsifying tablets were formulated with the objective of enhancing the bioavailability of non-steroidal anti-inflammatory drug, ibuprofen. Isotropic mixture of oil, surfactant was used to design the formulations. Goat fat and tween60 admixture was employed to formulate Self-emulsifying tablets (SETs). Different formulations were developed with varying composition of oil and surfactant by melt granulation technique and optimized. Resultant SETs were evaluated for weight variation, liquefaction time and drug content. The dissolution studies of ibuprofen from the SETs were determined in simulated gastric fluid without pepsin. Weight uniformity and absolute drug content was observed within the range in case of formulations made of admixtures of high proportion of tween60 and low content of goat fat. Liquefaction time determined was high but there was an every chance of reduction in liquefaction time due to gastric motility. Maximum drug release was shown for the formulations having high amount of tween60 and low content of goat fat. Dissolution studies revealed remarkable increase in release of the drug as compared to the standard drug. Results obtained indicated that ibuprofen could be conveniently administered in the form of SETs containing goat fat and tween60 admixtures.


Srinivasa Rao A.,Bhaskar Pharmacy College | Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Journal of Applied Pharmaceutical Science | Year: 2012

The aim of the study is to investigate the hepatoprotective activity of Melia azedarach L leaves extracts against simvastatin induced hepatotoxicity. The phytochemical screening was carried on the leaves extracts of Melia azedarach revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Melia azedarach was successively extracted with ethanol against simvastatin (20mg/kg.p.o) induced hepatotoxicity using Standard drug Silymarin (25 mg/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP),serum bilirubin and decrease the total proteins content.) in simvastatin treated rats, which were restored towards normalization in Melia azedarach (300 mg/kg and 500 mg/kg) treated animals. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.


Kiranmai M.,Nizam Institute of Pharmacy and Research Center | Mahendra Kumar C.B.,St. Mary's College | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

Azadirachta indica A. Juss. (AI) is a perinneal plant of family Maleacae.The total flavonoid content of AI was determined by using aluminium chloride coulorimetric method.It was found that Azadirachta indica root bark extracts contain total flavonoids in a range of 0.198 - 0.512 % g quercetin equivalent. The decoction method gave the highest yield (20.2%, w/w) of crude extract, while macertation extract gave the highest total flavonoid content (0.512 % g). Qualitative chemical tests for flavonoids and thin layer chromatographic analysis were used to screen the extracts obtained from different methods. Extracts obtained from maceration, microwave and soxhlet showed spots of R f values 0.92, 0.93, 0.93 corresponding to quercetin flavonoid which is found to be the most potent flavonoid.

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