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Srinivasa Rao A.,Bhaskar Pharmacy College | Ahmed M.F.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Journal of Applied Pharmaceutical Science | Year: 2012

The aim of the study is to investigate the hepatoprotective activity of Melia azedarach L leaves extracts against simvastatin induced hepatotoxicity. The phytochemical screening was carried on the leaves extracts of Melia azedarach revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Melia azedarach was successively extracted with ethanol against simvastatin (20mg/kg.p.o) induced hepatotoxicity using Standard drug Silymarin (25 mg/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP),serum bilirubin and decrease the total proteins content.) in simvastatin treated rats, which were restored towards normalization in Melia azedarach (300 mg/kg and 500 mg/kg) treated animals. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.


Kiranmai M.,Nizam Institute of Pharmacy and Research Center | Mahendra Kumar C.B.,St. Marys College | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

Azadirachta indica A. Juss. (AI) is a perinneal plant of family Maleacae.The total flavonoid content of AI was determined by using aluminium chloride coulorimetric method.It was found that Azadirachta indica root bark extracts contain total flavonoids in a range of 0.198 - 0.512 % g quercetin equivalent. The decoction method gave the highest yield (20.2%, w/w) of crude extract, while macertation extract gave the highest total flavonoid content (0.512 % g). Qualitative chemical tests for flavonoids and thin layer chromatographic analysis were used to screen the extracts obtained from different methods. Extracts obtained from maceration, microwave and soxhlet showed spots of R f values 0.92, 0.93, 0.93 corresponding to quercetin flavonoid which is found to be the most potent flavonoid.


Jayaveera K.N.,Jawaharlal Nehru University | Mohammed Fazil A.,Nizam Institute of Pharmacy and Research Center | Yasodha K.J.,Nizam Institute of Pharmacy and Research Center | Aleem M.A.,Nizam Institute of Pharmacy and Research Center | And 5 more authors.
American Journal of Clinical Hypnosis | Year: 2011

An aqueous root extract from Nardostachys jatamansi was investigated for its antioxidant and anticataleptic effects in the haloperidol-induced catalepsy rat model of the disease by measuring various behavioral and biochemical parameters. Catalepsy was induced by administration of haloperidol (1 mg/kg, ip) in male albino rats. A significant (P < 0.01) reduction in the cataleptic scores were observed in all the drug-treated groups as compared to the haloperidol-treated group; with maximum reduction observed in the Nardostachys jatamansi (250 and 500 mg/kg body weight) administered group. To estimate biochemical parameters: the generation of thiobarbituric acid reactive substances (TBARS); reduced glutathione (GSH) content and glutathione-dependent enzymes; catalase; and superoxide dismutase (SOD), in the brain were assessed. Haloperidol administration increased generation of TBARS and significantly reduced GSH, which were restored to near normal level with the Nardostachys jatamansi treatment. Catalase and SOD levels were also increased to normal levels, having been reduced significantly by haloperidol administration. Our findings of behavioral studies and biochemical estimations show that Nardostachys jatamansi reversed the haloperidol-induced catalepsy in rats. We conclude that the antioxidant potential has contributed to the reduction in the oxidative stress and catalepsy induced by haloperidol administration. © 2010 Rasheed et al, publisher and licensee Dove Medical Press Ltd.


Sattar M.A.,Pacific University at Udaipur | Iqbal M.M.,Nizam Institute of Pharmacy and Research Center | Syed Asad B.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

Hepatic and Renal systems are involved in the metabolism and excretion of various xenobiotics, environmental pollutants etc. Hence they are highly prone to attack by oxidative stress and generation of excessive concentration of free radicals. This results in tissue necrosis and damage of these organ systems. Keeping in view of its antioxidant and organ protective activities, the present research work is made by use of Albino Rats (Wister) to assess the influence of pre-treatment with methanolic extract of Phyllanthus amarus seeds and ethanolic extract of Leucas aspera leaves in simvastatin induced hepatotoxicity, where as in cisplatin induced nephrotoxicity. Liver and Kidney functions were assessed by collecting the blood samples from each group & evaluating the biochemical parameters. Histopathological studies were done by isolating the liver and kidney of all the groups. Treatment with methanolic and ethanolic extracts has brought back the elevated levels of serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), total & direct bilirubin, serum cholesterol and serum triglycerides in simvastatin induced hepatotoxic rats to near normal levels. Whereas methanolic extract, ethanolic extract and aqueous extract prevented the reduction in body weight and reduced the levels of total urea and serum creatinine in cisplatin induced nephrotoxicity. Histopathological observation exhibited the improved hepatic and renal anatomy that is reversed the damage. Thus methanolic extract of Phyllanthus amarus seeds and ethanolic extract of Leucas aspera leaves possess hepatoprotective and nephroprotective activities, comparatively Leucas aspera leaves shows better results, this may be due to presence of phenolic compounds like flavonoids steroids and tannins known to possess antioxidant activity.


Sri B.U.,Sri Padmavathi Mahila University | Kiranmai M.,Nizam Institute of Pharmacy and Research Center | Ibrahim M.,Nizam Institute of Pharmacy and Research Center | Muzib Y.I.,Sri Padmavathi Mahila University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

Self-emulsifying tablets were formulated with the objective of enhancing the bioavailability of non-steroidal anti-inflammatory drug, ibuprofen. Isotropic mixture of oil, surfactant was used to design the formulations. Goat fat and tween60 admixture was employed to formulate Self-emulsifying tablets (SETs). Different formulations were developed with varying composition of oil and surfactant by melt granulation technique and optimized. Resultant SETs were evaluated for weight variation, liquefaction time and drug content. The dissolution studies of ibuprofen from the SETs were determined in simulated gastric fluid without pepsin. Weight uniformity and absolute drug content was observed within the range in case of formulations made of admixtures of high proportion of tween60 and low content of goat fat. Liquefaction time determined was high but there was an every chance of reduction in liquefaction time due to gastric motility. Maximum drug release was shown for the formulations having high amount of tween60 and low content of goat fat. Dissolution studies revealed remarkable increase in release of the drug as compared to the standard drug. Results obtained indicated that ibuprofen could be conveniently administered in the form of SETs containing goat fat and tween60 admixtures.

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