Nizam Institute of Pharmacy

Nalgonda, India

Nizam Institute of Pharmacy

Nalgonda, India
SEARCH FILTERS
Time filter
Source Type

Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy | Kulkarni S.,Radiant Research Services Pvt Ltd | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Caralluma species have been reported for various traditional claims; the scientific data for possible activities have not yet been studied thoroughly. However the toxicity of a plant has to be studied for further exploration of various biological activities. Extracts were studied for their toxicity by MTT and trypan blue dye exclusion models against a panel of cancer, normal origin cell lines and EAC cells. The extracts found to be moderately toxic and showed dose dependent response.


Rasheed Ahemad S.,Nizam Institute of Pharmacy | Jayaveera K.N.,Jawaharlal Nehru Technological University Anantapur | Javeed M.Y.,Nizam Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Hydro alcoholic root extract from Nardostachys jatamansi was investigated for its antioxidant and anti cataleptic effects in the haloperidol-induced catalepsy rat model of the disease by measuring behavioral, biochemical parameters and neurotransmitter levels. Catalepsy was induced by administration of haloperidol (1 mg/kg, i.p) in male wistar rats. A significant (P < 0.01) reduction in the cataleptic scores were observed in all the drug-treated groups as compared to the haloperidol-treated group; with maximum reduction observed in the Nardostachys jatamansi (500 mg/kg body weight) administered group. Haloperidol administration increased generation of TBARS and significantly reduced GSH, which were restored to near normal level with the Nardostachys jatamansi treatment. Catalase and SOD levels were also increased to normal levels, having been reduced significantly by haloperidol administration. Haloperidol administration decreased the dopamine levels significantly, which were restored to near normal levels with the Nardostachys jatamansi treatment. Our findings of behavioral studies and biochemical estimations show that Nardostachys jatamansi reversed the haloperidol-induced catalepsy in rats.


Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy
BioImpacts | Year: 2014

Introduction: Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies. Methods: Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured. Results: The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase. Conclusion: The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action. © 2014 The Author(s).


Kiranmai M.,Nizam institute of pharmacy | Mahender Kumar C.B.,St. Mary's College | Ibrahim M.D.,Nizam institute of pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Purpose: The aim of this study was to investigate the antioxidant activity of the root bark extract from the neem tree (Azadirachta indica A. Juss var., Meliaceae) using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay. Method: The root bark was extracted by soxhlet extraction method using 80% ethanol and the resultant extract was subjected to free radical scavenging activity by DPPH assay and total antioxidant activity. Qualitative analysis and thin-layer chromatography of the extract was also investigated. Result: The results showed that the root bark extract exhibited higher free radical scavenging effect on the DPPH assay with 50% scavenging activity at 27.3 μg/ml. The total antioxidant activity of this extract was found to be 0.58 mM of standard ascorbic acid. Conclusion: The results suggested that root bark extract of the neem tree have strong antioxidant potential. Thin-layer chromatography showed spot corresponding to quercetin.


Avanapu S.R.,Bhaskar Pharmacy College | Dachani S.R.,Nizam Institute of Pharmacy | Ananth P.H.,Nizam Institute of Pharmacy
International Journal of Phytomedicine | Year: 2012

The aim of the present study was to investigate the antioxidant and renal protective effect of Trichodesma amplexicaule extract in streptozotocin-induced diabetic rats. Wistar rats were randomly divided into five groups: Normal control, diabetic control and Trichodesma amplexicaule (100 and 200mg/kg) and glibenclamide treated groups. Blood glucose, Total Cholesterol, Triglycerides, Aminotransferases, kidney functions and kidney oxidant/antioxidants status were estimated. Streptozotocin effectively altered the biochemical, histoarchitecture, malondialdehyde and antioxidant enzyme levels, while Trichodesma amplexicaule ameliorated their levels in the treated group. Oral treatment with methanolic extract for 4 weeks reduced significantly Blood glucose, Total Cholesterol, Triglycerides, Aminotransferases, kidney function parameter levels in diabetic animals. Furthermore, the biochemical findings were supplemented with histopathological examination of pancreas and kidney. Histological observation of kidney of diabetic rats showed degenerative changes in glomerulus and renal tubules and extract treatment rejuvenated kidney histoarchitecture. Results of this study suggest that Trichodesma amplexicaule offered significant protection against oxidative stress induced by Streptozotocin, as noted by increase in superoxide dismutase and catalase along with decrease in lipid peroxidation levels. The present work provide scientific evidence of the preventive and therapeutic potential of Trichodesma amplexicaule against kidney disorder in diabetes and thus may provide a promising drug for managing diabetic kidney disorders and assumes that activity may be due to the prominent antioxidant property shown by Trichodesma amplexicaule.


Dachani S.R.,Nizam Institute of Pharmacy | Ananth P.H.,Nizam Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2012

The goal of the present study was to provide in vitro evidences for antioxidant and anti diabetic potential of Trichodesma indicum to generate a stronger biochemical rationale for further in vivo studies. The hydroalchohol extract of Trichodesma indicum whole plant was screened for its in vitro antioxidant activity using 2, 2-Diphenly 1-picryl hydrazyl assay, Scavenging of Nitric oxide and Superoxide radical methods, evaluation of Total antioxidant capacity of the extract and Reducing power assay. Cytotoxic effect of the extract was evaluated by MTT assay and in-vitro antidiabetic effect was studied using the glucose uptake model in rodent skeletal muscle cells (L-6 cells) involved in glucose utilization. The extract of Trichodesma indicum showed good antioxidant properties against 2, 2-Diphenly 1-picryl hydrazyl radical with low IC50 values 135μg/ml and against nitric oxide and superoxide radical exhibited poor scavenging properties with IC50 value of > 1000μg/ml. The total antioxidant activity which expressed equivalent to Ascorbic acid has 225.28 mg per gram of dried extract. The increased absorbance at 700 nm indicates an increase in reducing power of samples. The drug extract showed percentage growth inhibition value of 500 4g/ml and showed average glucose uptake (P<0.05) with percentage of glucose uptake of 91.03±10.12 over the control. The extract of Trichodesma indicum exhibited significant antioxidant activity and moderate antidiabetic activity and merits further investigation in animal models and support traditional claim.


Kodithala S.,Nizam institute of pharmacy | Prakash Yoganandam G.,Government of Puducherry | Kiranmai M.,Nizam Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2013

Objective: The present study is designed to study the pharmacognostical, preliminary phytochemical and anti cancer studies of Dendrophthoe falcata (L.f) Ettingsh (Loranthaceae) on the host plant Azadirachta indica(Meliaceae). Materials and methods: Pharmacognostical studies involve the anatomical sections and powder analysis of leaf, physicochemical studies such as extractive values, ash values and fluorescent analysis were performed. For methanolic extract preliminary phytochemical studies revealed the presence of carbohydrates, glycosides, proteins, gums, mucilages, tannins and saponins. These findings will be useful towards establishing standards on identification, purity, quality and classification of the plant, which is gaining relevance in the plant drug research. Methanolic extract of the leaf prepared by soxhlet method of extraction was subjected to in vitro and in vivo (Ehrlich ascitic carcinoma cells) anti cancer activities. Results: The methanolic extract of Dendrophthoe falcata was evaluated and compared against standard drug 5-flourouracil. 100mg/kg of methanolic extract (MEDF) shows significant anticancer activity which was in correlation with the presence of phenolic and flavonoid constituents of the extract. Conclusion: It was concluded that the anti cancer activity due to the presence of alkaloids, phenolics and flavonoids which are rich in D.falcata which grows on host plant Azadirachta indica, it may be because of the host plant which is rich in flavonoids and phenolics.


Srinivasarao M.,Nizam Institute of Pharmacy | Pradeep H.A.,Nizam Institute of Pharmacy | Ibrahim M.,Nizam Institute of Pharmacy | Ibrahim M.,Asian Medical Institute
International Journal of Pharma and Bio Sciences | Year: 2013

The cytochrome P450 protective activity of Sapindus mukorossi and Rheum emodi were evaluated against CCl4 induced prolongation of pentobarbitone sleep time in Wister rats. The fractions, at a dose of 0.2 g/kg,0.4g/kg p.o., Sapindus mukorossi, Rheum emodi significantly prevents the CCl4 induced prolongation of pentobarbitone sleep time (P<0.01), indicating the cytochrome P450 protection activity. However, when treated alone, the fractions (0.2 g/kg and 0.4 g/kg, p.o.) shows a significant prolongation of pentobarbitone sleep time (P<0.01), indicating the intrinsic cytochrome P450 inhibition activity. In addition, the extracts show neither stimulant nor depressant effect on the CNS, as evident from locomotor activity test. In conclusion, the results indicate that, the Sapindus mukorossi saponin fraction and anthraquinone fraction of rheum emodi at an oral dose of 0.2 g/kg and 0.4 g/kg, p.o., shows significant protective effects against CCl4 induced cytochrome P450 damage and also show a significant intrinsic cytochrome P450 inhibition activity.


PubMed | Nizam Institute of Pharmacy
Type: | Journal: Diabetes, metabolic syndrome and obesity : targets and therapy | Year: 2011

The roots of Holostemma annularis K. Schum (Asclepiadacae) are used in traditional medicine to treat diabetes. This medicinal plant, widely used in more than 34 ayurvedic preparations, was evaluated in a high fructose diet in induced insulin resistance and in C57BL/6J ob/ob diabetic mice for its antidiabetic activity. Graded doses of both chloroform and methanolic extracts of the roots of H. annularis were administered to normal and experimental diabetic rats for 21 days. Serum glucose, triglycerides, cholesterol levels and total protein in urine were analyzed. Significant results were observed in the estimated parameters. These data justify the use of the plant in the treatment of diabetes mellitus and is a potential source for the isolation of new active agents for diabetes mellitus.


PubMed | Nizam Institute of Pharmacy and Radiant Research Services Pvt Ltd
Type: Journal Article | Journal: BioImpacts : BI | Year: 2014

Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies.Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured.The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase.The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action.

Loading Nizam Institute of Pharmacy collaborators
Loading Nizam Institute of Pharmacy collaborators