Nizam Institute of Pharmacy

Nalgonda, India

Nizam Institute of Pharmacy

Nalgonda, India
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Ibrahim M.,Nizam Institute of Pharmacy | Kiranmai M.,Nizam Institute of Pharmacy
International Journal of Green Pharmacy | Year: 2012

Background: Plant-based or plant-derived drugs occupied 30% of the modern system of medicine. Several trees possess a variety of biologically active compounds. Among them, Azadirachta indica, belonging to the family Meliaceae, plays a vital role as it acts as nature′s pharmacy from several centuries of time. In the path of searching for potential antioxidants from plant origin, different parts of Azadirachta indica have been selected. Aim: Successive solvent extracts of leaves, seeds and root barks of Azadirachta indica A. Juss (neem) were studied for their free radical scavenging activity. Materials and Methods: Extraction capacity of different solvents based on their polarity order was compared and the extracts were subjected to 1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging assay. Statistical Analysis: All values were expressed in meanSEM and correlation coefficient (R 2 ) values obtained from dose response curves were expressed for antioxidant results. Results: The results showed that the highest yields was found with the hydroalcoholic extract of leaves, followed by the hydroalcoholic extract of seeds and methanolic extracts of leaves and seeds, respectively. Free radical scavenging activity of various extracts was determined by measuring 1, DPPH free radical scavenging activity. The results were expressed in terms of IC 50 values. The root bark methanol extract was found to exhibit the highest IC 50 of 14.82-g/mL at the concentration of 25-g/mL. Conclusion: It is concluded that hydroalcohol and methanol are the best solvents to extract the antioxidant compounds from Azadirachta indica. The root bark methanolic extract was found to have the highest free radical scavenging potential against DPPH radical.


Rasheed Ahemad S.,Nizam Institute of Pharmacy | Mohammed F.A.,Nizam Institute of Pharmacy | Jayveera K.N.,Jawaharlal Nehru Technological University Anantapur
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Ethanolic root extract from Smilax zeylanica was investigated for its antioxidant and anti cataleptic effects in the haloperidol-induced catalepsy rat model of the disease by measuring behavioral and biochemical parameters. Catalepsy was induced by administration of haloperidol (1 mg/kg, i.p) in male albino rats. A significant (P < 0.01) reduction in the cataleptic scores were observed in all the drug-treated groups as compared to the haloperidol-treated group; with maximum reduction observed in the Smilax zeylanica (500 mg/kg body weight) administered group. To estimate biochemical parameters: the generation of thiobarbituric acid reactive substances (TBARS); reduced glutathione (GSH) content and glutathione-dependent enzymes; catalase; and superoxide dismutase (SOD), in the brain were assessed. Haloperidol administration increased generation of TBARS and significantly reduced GSH, which were restored to near normal level with the Smilax zeylanica treatment. Catalase and SOD levels were also increased to normal levels, having been reduced significantly by haloperidol administration. Our findings of behavioral studies and biochemical estimations show that Smilax zeylanica reversed the haloperidol-induced catalepsy in rats. We conclude that the antioxidant potential has contributed to the reduction in the oxidative stress and catalepsy induced by haloperidol administration.


Usha Sri B.,Nizam Institute of Pharmacy | Indira Muzib Y.,Sri Padmavathi Mahila Viswavidyalayam
International Journal of Drug Delivery | Year: 2015

The main objective of present work was to prepare a solid SEDDS for enhancement of oral bioavailability of Valsartan, poorly water soluble drug. The solubility of the drug was determined in various vehicles. A pseudo ternary phase diagram was constructed to identify the self-micro emulsification region. Further, the resultant formulations were investigated for clarity, phase separation, globule size, effect of pH and dilutions and freeze-thaw stability. The optimized SMEDDS (F4) formulation of Valsartan contained Capmul MCM (Oil), Kolliphor HS 15 (Surfactant) and PEG 400 (Co-surfactant). This optimized formulation was converted in to solid SEDDS by adding required quantity of Neusilin US2 as adsorbing agent used for in vitro dissolution and bioavailability assessment. The oral bioavailability of Valsartan from solid SEDDS was 1.6-fold higher compared to that of Valsartan suspension in rats, suggesting a significant increase (p < 0.05) in oral bioavailability of Valsartan from solid SEDDS. © 2014, Advanced Research Journals. All rights reserved.


Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy | Kulkarni S.,Radiant Research Services Pvt Ltd | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Caralluma species have been reported for various traditional claims; the scientific data for possible activities have not yet been studied thoroughly. However the toxicity of a plant has to be studied for further exploration of various biological activities. Extracts were studied for their toxicity by MTT and trypan blue dye exclusion models against a panel of cancer, normal origin cell lines and EAC cells. The extracts found to be moderately toxic and showed dose dependent response.


Rasheed Ahemad S.,Nizam Institute of Pharmacy | Jayaveera K.N.,Jawaharlal Nehru Technological University Anantapur | Javeed M.Y.,Nizam Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Hydro alcoholic root extract from Nardostachys jatamansi was investigated for its antioxidant and anti cataleptic effects in the haloperidol-induced catalepsy rat model of the disease by measuring behavioral, biochemical parameters and neurotransmitter levels. Catalepsy was induced by administration of haloperidol (1 mg/kg, i.p) in male wistar rats. A significant (P < 0.01) reduction in the cataleptic scores were observed in all the drug-treated groups as compared to the haloperidol-treated group; with maximum reduction observed in the Nardostachys jatamansi (500 mg/kg body weight) administered group. Haloperidol administration increased generation of TBARS and significantly reduced GSH, which were restored to near normal level with the Nardostachys jatamansi treatment. Catalase and SOD levels were also increased to normal levels, having been reduced significantly by haloperidol administration. Haloperidol administration decreased the dopamine levels significantly, which were restored to near normal levels with the Nardostachys jatamansi treatment. Our findings of behavioral studies and biochemical estimations show that Nardostachys jatamansi reversed the haloperidol-induced catalepsy in rats.


Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy
BioImpacts | Year: 2014

Introduction: Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies. Methods: Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured. Results: The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase. Conclusion: The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action. © 2014 The Author(s).


Kiranmai M.,Nizam institute of pharmacy | Mahender Kumar C.B.,St. Mary's College | Ibrahim M.D.,Nizam institute of pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Purpose: The aim of this study was to investigate the antioxidant activity of the root bark extract from the neem tree (Azadirachta indica A. Juss var., Meliaceae) using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay. Method: The root bark was extracted by soxhlet extraction method using 80% ethanol and the resultant extract was subjected to free radical scavenging activity by DPPH assay and total antioxidant activity. Qualitative analysis and thin-layer chromatography of the extract was also investigated. Result: The results showed that the root bark extract exhibited higher free radical scavenging effect on the DPPH assay with 50% scavenging activity at 27.3 μg/ml. The total antioxidant activity of this extract was found to be 0.58 mM of standard ascorbic acid. Conclusion: The results suggested that root bark extract of the neem tree have strong antioxidant potential. Thin-layer chromatography showed spot corresponding to quercetin.


Dachani S.R.,Nizam Institute of Pharmacy | Ananth P.H.,Nizam Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2012

The goal of the present study was to provide in vitro evidences for antioxidant and anti diabetic potential of Trichodesma indicum to generate a stronger biochemical rationale for further in vivo studies. The hydroalchohol extract of Trichodesma indicum whole plant was screened for its in vitro antioxidant activity using 2, 2-Diphenly 1-picryl hydrazyl assay, Scavenging of Nitric oxide and Superoxide radical methods, evaluation of Total antioxidant capacity of the extract and Reducing power assay. Cytotoxic effect of the extract was evaluated by MTT assay and in-vitro antidiabetic effect was studied using the glucose uptake model in rodent skeletal muscle cells (L-6 cells) involved in glucose utilization. The extract of Trichodesma indicum showed good antioxidant properties against 2, 2-Diphenly 1-picryl hydrazyl radical with low IC50 values 135μg/ml and against nitric oxide and superoxide radical exhibited poor scavenging properties with IC50 value of > 1000μg/ml. The total antioxidant activity which expressed equivalent to Ascorbic acid has 225.28 mg per gram of dried extract. The increased absorbance at 700 nm indicates an increase in reducing power of samples. The drug extract showed percentage growth inhibition value of 500 4g/ml and showed average glucose uptake (P<0.05) with percentage of glucose uptake of 91.03±10.12 over the control. The extract of Trichodesma indicum exhibited significant antioxidant activity and moderate antidiabetic activity and merits further investigation in animal models and support traditional claim.


Kodithala S.,Nizam institute of pharmacy | Prakash Yoganandam G.,Government of Puducherry | Kiranmai M.,Nizam Institute of Pharmacy
International Journal of Pharma and Bio Sciences | Year: 2013

Objective: The present study is designed to study the pharmacognostical, preliminary phytochemical and anti cancer studies of Dendrophthoe falcata (L.f) Ettingsh (Loranthaceae) on the host plant Azadirachta indica(Meliaceae). Materials and methods: Pharmacognostical studies involve the anatomical sections and powder analysis of leaf, physicochemical studies such as extractive values, ash values and fluorescent analysis were performed. For methanolic extract preliminary phytochemical studies revealed the presence of carbohydrates, glycosides, proteins, gums, mucilages, tannins and saponins. These findings will be useful towards establishing standards on identification, purity, quality and classification of the plant, which is gaining relevance in the plant drug research. Methanolic extract of the leaf prepared by soxhlet method of extraction was subjected to in vitro and in vivo (Ehrlich ascitic carcinoma cells) anti cancer activities. Results: The methanolic extract of Dendrophthoe falcata was evaluated and compared against standard drug 5-flourouracil. 100mg/kg of methanolic extract (MEDF) shows significant anticancer activity which was in correlation with the presence of phenolic and flavonoid constituents of the extract. Conclusion: It was concluded that the anti cancer activity due to the presence of alkaloids, phenolics and flavonoids which are rich in D.falcata which grows on host plant Azadirachta indica, it may be because of the host plant which is rich in flavonoids and phenolics.


PubMed | Nizam Institute of Pharmacy and Radiant Research Services Pvt Ltd
Type: Journal Article | Journal: BioImpacts : BI | Year: 2014

Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies.Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured.The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase.The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action.

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