Rao S.,Nizam College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013
Six drugs viz., Astemizole, Domperidone, Esomeprazole, Losartan potassium, Sumatriptan and Terazosin were tested for the formation of charge transfer complexes with 2, 3-dichloro 5, 6-dicyano- p- benzoquinone, (DDQ). Each of these drugs turned the pale yellow colour of reagent i.e DDQ. in CH3CN, to purple and exhibited three bands at 455, 545 and 588 nm due to anion of the reagent whose intensity increased with increase in the concentration of the drugs and formed a basis for quantitative determination of the drugs. The complexes were found to have 1:1 composition and have stability of the order 103 to 104 The effect of the concentration reagent, polarity of solvent, interferenceof excipients have been studied and optimized. Acetonitrile was found to be suitable solvent for the analysis. The methods have been validated in terms of ICH guidelines and applied to the quantification of pharmaceuticals. The variation of slopes of calibration plots and stability constants of the complexes are discussed in terms of structures of the drugs.
Pandey V.,Nizam College
International Journal of Applied Engineering Research | Year: 2014
In the aftermath of Globalization with relatively easy trans border movement of people, there is diffusion of material and non-material culture. With specific reference to Hyderabad, the diffusion of various cultures in Hyderabad has led to a unique way of life in cities which has contributed to further consolidation and development of cosmopolitanism. On the other hand Globalization indirectly has threatened the regional identities of people and places. This has resulted in the people associating with their regional specific identities in a very rigid manner resulting in increase in regional conflicts world over. The present research study tries to present the contrasts of cosmopolitanism and regionalism; their influence on urban development and how regionalism in its extreme form leads to regional conflicts. © Research India Publications.
Veerappan R.,Rajah Serfoji Government College |
Senthilkumar R.,Nizam College
International Journal of Nutrition, Pharmacology, Neurological Diseases | Year: 2015
Objectives: The study seeks to evaluate the effect of chrysin; a natural, biologically active compound extracted from plants, honey or propolis, on the tissues and circulatory antioxidant status and lipid peroxidation in Nω-nitro-l-arginine methyl ester (L-NAME) induced hypertensive rats. Materials and Methods: Male albino rats were divided into four groups. Control (Group I) and chrysin supplementation of the control (Group II) received normal diet. Groups III and IV received L-NAME (40 mg/kg B.W). Groups II and IV received chrysin (25 mg/kg B.W) dissolved in 0.2% dimethylsulfoxide solution after the 4 th week. Results and Discussion: The results showed significantly elevated levels of tissue and circulatory thiobarbituric acid reactive substances, conjugated dienes and lipid hydroperoxides, and significantly lowered enzymic and non-enzymic antioxidant activity of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C and vitamin E in L-NAME-induced hypertensive rats compared with those in control group. From chrysin administration to rats with L-NAME-induced hypertension leads to tissue damage which significantly decreases the levels of thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes, and significantly elevates the activity of superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, vitamin C and vitamin E in the tissues and circulation compared with those on the unsupplemented L-NAME induced hypertensive group. Conclusions: Chrysin offers protection against free radical-mediated oxidative stress in rats with L-NAME-induced hypertension. © 2015, Wolters Kluwer - Medknow Publications. All rights reserved.
Rajagopal S.,Nizam College |
Fields B.L.,Norfolk State University |
Burton B.K.,Norfolk State University |
On C.,Norfolk State University |
And 2 more authors.
Neuroscience | Year: 2014
Cav2.2 channels are a substrate for phosphorylation by protein kinase C (PKC) isozymes. The contribution of Cavβ, an auxiliary subunit of these channels, in the PKC modulation was studied. Cav2.2 channels were expressed in Xenopus oocytes in various subunit combinations with or without Cavβ subunits. Currents were recorded using a two-electrode voltage clamp with barium as the charge carrier (IBa). Acetyl-β-methylcholine (MCh), an activator of PKCα, potentiated Cav2.2 currents expressed with Cav2.2α1 alone or Cav2.2α1α2/δ. Similarly PKC isozymes α, βII or e potentiated IBa through Cav2.2α1 subunit channels. In contrast, MCh failed to potentiate currents expressed with Cav2.2α1 and Cavβ1b, β2a, β3 or β4 subunits. Similarly, in the presence of Cavβ1b subunits, PKC isozymes failed to potentiate these currents; contrarily, PKCs α or βII decreased the IBa. MCh failed to potentiate Cav2.2α1 subunit currents in the serine/threonine (Ser/Thr)→alanine mutants, T422A, S1757A or S2132A of Cav2.2α1 subunits. Hence Thr-422, Ser-1757 and Ser-2132 may be PKCα isozyme target sites. The action of PKC on these sites was further substantiated by the increased basal IBa along with the loss of MCh potentiation when Ser/Thr was mutated to aspartate. The observation that MCh or PKC isozymes failed to affect Cav2.2 currents in the presence of Cavβ subunits suggests that these subunits may have interfered with the interaction between PKC and Ser/Thr sites of Cav2.2α1 subunits. In addition to affecting channel expression and current kinetics, Cavβ subunits may also modulate the response of these channels to neurochemicals. © 2014 IBRO.
Maiti B.,Banaras Hindu University |
Rathore A.,Nizam College |
Srivastava S.,National Institute of Technology Durgapur |
Shekhawat M.,Banaras Hindu University |
Srivastava P.,Banaras Hindu University
Applied Microbiology and Biotechnology | Year: 2011
Ethanol is a potential energy source and its production from renewable biomass has gained lot of popularity. There has been worldwide research to produce ethanol from regional inexpensive substrates. The present study deals with the optimization of process parameters (viz. temperature, pH, initial total reducing sugar (TRS) concentration in sugar cane molasses and fermentation time) for ethanol production from sugar cane molasses by Zymomonas mobilis using Box-Behnken experimental design and genetic algorithm (GA). An empirical model was developed through response surface methodology to analyze the effects of the process parameters on ethanol production. The data obtained after performing the experiments based on statistical design was utilized for regression analysis and analysis of variance studies. The regression equation obtained after regression analysis was used as a fitness function for the genetic algorithm. The GA optimization technique predicted a maximum ethanol yield of 59.59 g/L at temperature 31 °C, pH 5.13, initial TRS concentration 216 g/L and fermentation time 44 h. The maximum experimental ethanol yield obtained after applying GA was 58.4 g/L, which was in close agreement with the predicted value. © 2011 Springer-Verlag.