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Castel Guelfo di Bologna, Italy

Cleton N.B.,Erasmus Medical Center | Cleton N.B.,National Health Research Institute | Godeke G.-J.,National Health Research Institute | Reimerink J.,National Health Research Institute | And 17 more authors.
PLoS Neglected Tropical Diseases | Year: 2015

The family Flaviviridae, genus Flavivirus, holds many of the world’s most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins for detection of medically important viruses within the genus Flavivirus. Sera from clinical flavivirus patients were used for primary development of the protein microarray. Results show a high IgG and IgM sensitivity and specificity for individual NS1 antigens, and limited cross reactivity, even within serocomplexes. In addition, the serology based on this array allows for discrimination between infection and vaccination response for JEV vaccine, and no cross-reactivity with TBEV and YFV vaccine induced antibodies when testing for antibodies to other flaviviruses Based on these data, multiplex NS1-based protein microarray is a promising tool for surveillance and diagnosis of flaviviruses. Source


Farella E.,niversity of Bologna | Ricc B.,niversity of Bologna
Microelectronics Journal | Year: 2010

"Casattenta" (Aware home, in Italian) is the demonstrator of a research project on "Ambient Intelligence", "Sensor Fusion" and "Wireless Sensor Networks". The result is a system composed of fixed and wearable sensor nodes, providing elderly people living alone in their house (but also people in other situations and environments) with adequate and non-intrusive monitoring aimed at improving their safety and quality of life. The system consists of fixed smart sensors distributed in the environment and wearable ones monitoring the inhabitants' health and activity. The interaction between fixed and mobile nodes, based on the ZigBee compliant technologies, allows indoor tracking and identification of dangerous events. © 2010 Elsevier Ltd. All rights reserved. Source

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