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Vidya S.M.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | Krishna V.,Kuvempu University | Rajesh K.P.-G.,Kuvempu University | Bharath B.R.,Kuvempu University | Manjunatha H.,Kuvempu University
Medicinal Chemistry Research

In vitro antibacterial activity of a phytoconstituent, entagenic acid isolated from the seed kernel of Entada pursaetha DC was screened against both gram negative and gram positive bacteria. The entagenic acid showed the high antibacterial activity against B. cereus and B. subtilis with minimal inhibitory concentration of 200 μg ml -1 and possesses better glucosamine-6-phosphate synthase inhibition in molecular docking studies with minimum docking energy -9.22 kJ mol -1, binding energy -9.28 kJ mol -1 and inhibition constant 1.57e-007. The inhibition constant of streptomycin was 3.86e-005. © Springer Science+Business Media, LLC 2011. Source

Annappa P.H.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | Shenoy U.K.K.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology
Advances in Intelligent Systems and Computing

Wireless mesh networks (WMN) possess characteristics such as self-healing, self-configuring and self-discovery. Due to this nature WMN has emerged as the most widely used popular network. Since these devices are operated using battery resources, several works have been carried out for minimizing energy consumption during routing process, thereby increasing network lifetime. WMNs are more vulnerable for attackers due to its wide usage. Many works can be found to detect the intruder during routing without considering energy as a metric. There exist possibilities of intruder to attack the battery resource thereby reducing network efficiency in energy-aware routing. Hence in this work we propose a novel approach to detect an intruder by self-monitoring mechanism of node considering metrics such as packet size, data rate, remaining energy and draining rate of a energy resources of a node. The proposed model consists of three modules, namely self-intrusion detector, monitor and evaluator. It detects and helps in making decisions to participate in the network transmission. The working of the model is analyzed and shows that the proposed model detects intruder effectively, thereby resulting in increase of WMN efficiency. © Springer India 2016. Source

Nayak V.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | Pai P.V.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | Pai A.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | Pai S.,Nitte Mahalinga Adyanthaya Memorial Institute of Technology | And 2 more authors.
Bioremediation Journal

The objective of the present study is to investigate the caffeine-degrading abilities of different fungi and to apply this knowledge to environmental remediation and industrial decaffeination process. Chrysosporium keratinophilum, Gliocladium roseum, Fusarium solani, and Aspergillus restrictus were isolated from the coffee pulp obtained from a coffee estate. Pure cultures of fungi were isolated on standard conventional potato dextrose broth (PDB) medium and authenticated. Pure cultures were subjected to a caffeine tolerance study at different concentrations of caffeine (1-8 g/L) in potato dextrose agar (PDA) and minimal media. On PDA, Fusarium solani could tolerate caffeine concentration up to 8 g/L, whereas Chrysosporium keratinophilum, Gliocladium roseum, and Aspergillus restrictus could tolerate up to 6 g/L. On minimal agar medium containing different concentrations of caffeine (1-8 g/L), Fusarium solani tolerated up to 8 g/L and the other fungi up to 2 g/L. A time-bound caffeine degradation study was undertaken at 1 g/L concentration of caffeine and glucose in nitrogen-containing and nitrogen-free liquid minimal media by subjecting the four fungi to shake flask culture at 120 rpm and 30°C. Degradation of caffeine up to 7 days at 24-h intervals was analyzed by high-performance liquid chromatography (HPLC). Gliocladium roseum followed by Aspergillus restrictus showed maximum degradation of caffeine at 0.47 and 0.3 mg/ml, respectively, by 96 h in nitrogen-containing minimal medium, whereas Fusarium solani showed maximum degradation of caffeine by 48 h (0.35 mg/ml) and Chrysosporium keratinophilum by 72 h (0.29 g/ml). In nitrogen-free minimal medium, Chrysosporium keratinophilum showed maximum degradation of caffeine at 72 h (0.45 mg/ml), followed by Gliocladium roseum, Fusarium solani (0.3 mg/ml), and Aspergillus restrictus (0.25 mg/ml) at 96 h. Overall, Chrysosporium keratinophilum showed a comparatively higher rate of caffeine degradation in minimal medium with or without a nitrogen source as compared with the other three fungi, indicating that nitrogen affects caffeine metabolism. © 2013 Taylor & Francis Group, LLC. Source

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