Patil V.M.,Nirmal Seeds Pvt. Ltd |
Dhande G.A.,Nirmal Seeds Pvt. Ltd |
Thigale D.M.,Nirmal Seeds Pvt. Ltd |
Rajput J.C.,Nirmal Seeds Pvt. Ltd
African Journal of Biotechnology | Year: 2011
Reliable and reproducible protocols to get healthy and well formed plants from nodal explants of the pomegranate (Punica granatum L) cv 'Bhagava' has been developed. Nodal segments were cultured on two different media at full strength Murashige and Skoog (MS) and Woody Plant Medium (WPM). The media was prepared as a basal medium supplemented with 0.2 to 2 mg/L 6-benzylaminopurine (BAP), 0.1 to 1 mg/L 1-naphthalacetic acid (NAA), 0.5 to 2.5 mg/L silver nitrate (AgNO 3) and 10 to 50 mg/L adenine sulphate for establishment stage. For proliferation stage, 0.1 to 0.5 mg/L BAP and NAA was tested. For rooting stage 0.0, 0.25 and 0.5 mg/L 3-Indolebutyric acid (IBA) and NAA on MS and WPM medium were tested. The nodal explants grown on MS medium containing 1.8 mg/L BAP, 0.9 mg/L NAA, 1 mg/L silver nitrate and 30 mg/L adenine sulphate had the highest proliferation rate (10 to 15 shoots/explants) in establishment stage. The same trend was found concerning the maximum leaves numbers (15 to 20 leaves/explants) on proliferation medium containing 0.4 mg/L BAP and 0.3 mg/L NAA. The plantlets grown on MS medium were found to have better survival compared to WPM medium. 0.5 mg/L NAA and 0.5 mg/L IBA showed equal rooting response in both the medium, whereas thick root formation was observed in the medium containing IBA. © 2011 Academic Journals.
Deshmukh N.D.,Nirmal Agricultural Research and Development Foundation |
Deshmukh N.D.,Nirmal Seeds Pvt. Ltd |
Jadhav B.P.,Nirmal Agricultural Research and Development Foundation |
Jadhav B.P.,Nirmal Seeds Pvt. Ltd |
And 4 more authors.
Pestology | Year: 2010
Okra (Abelmoschus exculentus L.) is a major and widely grown vegetable crop in India. The existing commercial varieties/hybrids were observed to be susceptible to YVMV disease. Even the wild Abelmoschus spp. showed susceptibility. Generation of new variability for future crop improvement were the major task before the vegetable scientists. Systematic research resulted in new variability in okra which was taken for studies. An experiment was conducted to evaluate the resistant sources among the new developed genotypes. Thirty five open pollinated genotypes of okra were extensively screened against yellow vein mosaic virus for four successive years (2004-2007) o\er two seasons per year i.e. kharif and summer, at Nirmal Agricultural Research and Development Foundation (NARD), Bhadgaon, Dist-Jalgaon (Maharashtra). Among the genotypes, NOL-285 was found absolutely free (HR) from YVMV disease, whereas NOL-303, NOL-145 showed moderate reaction (R). On the other hand, NOL-2-1, NOL-364 and NOL-260 showed severe reaction. It was also observed that per cent yellow vein mosaic virus incidence was higher in summer season than that of kharif. Moreover, the incidence of YVMV in different genotypes showed great variation from season to season and year to year.