Nippon Flour Mills Co.

Atsugi, Japan

Nippon Flour Mills Co.

Atsugi, Japan

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Suchy J.,Canadian Grain Commission | Dupuis B.,Canadian Grain Commission | Sakamoto J.,Nippon Flour Mills Co. | Fu B.X.,Canadian Grain Commission
Cereal Chemistry | Year: 2017

The extensigraph is an internationally accepted method for measurement of physical properties of dough subjected to mechanical handling and resting. Standard extensigraph methods (AACC International Approved Method 54-10.01, ISO 5530-2) use the farinograph for the preparation of dough in the presence of 2% salt at reduced water absorption (farinograph absorption minus 2-3%). However, the dough so prepared is usually underdeveloped and drier than typically seen in common baking processes. In addition, the standard extensigraph test is time consuming and requires a large sample size. In this study, an alternate dough preparation protocol is proposed, consisting of a Swanson-type pin mixer at reduced salt (1%) and elevated water absorption (farinograph absorption plus 4%). With the alternate method, dough is fully developed and similar to bread dough in physical properties. AACCI Approved Method 54-10.01 is followed for dough rounding, molding, resting, and stretching by using the Brabender Extensograph-E instrument. Strong correlations for resistance to extension (r = 0.90) and area (r = 0.92) were found between the modified and standard dough preparation methods. This protocol requires much less flour sample and significantly increases sample throughput. © 2017 AACC International, Inc.


Patent
Nisshin Seifun Group Inc. and Nippon Flour Mills Co. | Date: 2010-12-17

Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.


Sugawara T.,Kyoto University | Duan J.,Kyoto University | Aida K.,Nippon Flour Mills Co. | Tsuduki T.,Tohoku University | Hirata T.,Kyoto University
Lipids | Year: 2010

We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants. © 2010 AOCS.


Sugawara T.,Kyoto University | Tsuduki T.,Tohoku University | Yano S.,Kyoto University | Hirose M.,Kyoto University | And 4 more authors.
Journal of Lipid Research | Year: 2010

Sphingolipids are ubiquitous in all eukaryotic organisms. Various physiological functions of dietary sphingolipids, such as preventing colon cancer and improving the skin barrier function, have been recently reported. One of the common sphingolipids used as a foodstuff is glucosylceramide from plant sources, which is composed of sphingoid bases distinct from those of mammals. However, the fate of dietary sphingolipids derived from plants is still not understood. In this study, we investigated the absorption of maize glucosylceramide in the rat intestine using a lipid absorption assay of lymph from the thoracic duct. The free and complex forms of trans-4, cis-8-sphingadienine, the predominant sphingoid base of maize glucosylceramide, were found in the lymph after administration of maize glucosylceramide. This plant type of sphingoid base was detected in the ceramide fraction and N-palmitoyl-4,8-sphingadienine (C16:0-d18:2) and N-tricosanoyl-4,8- sphingadienine (C23:0-d18:2) were identified by LC-MS/MS. The cumulative recovery of 4t,8c-sphingadienine in the lymph was very low. These results indicate that dietary glucosylceramide originating from higher plants is slightly absorbed in the intestine and is incorporated into ceramide structures in the intestinal cells.jlr However, it appears that the intact form of sphingoid bases is not reutilized well in the tissues. Copyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc.


Duan J.,Kyoto University | Sugawara T.,Kyoto University | Hirose M.,Kyoto University | Aida K.,Nippon Flour Mills Co. | And 3 more authors.
Experimental Dermatology | Year: 2012

Sphingolipids are ubiquitous in eukaryotic organisms and are significant components in foods. It has been reported that treatment with sphingolipids prevents colon cancer, improves skin barrier function and suppresses inflammatory responses. However, the mechanisms for those effects of dietary sphingolipids are not well understood. In this study, to investigate the effects of dietary glucosylceramide (GluCer) and sphingomyelin (SM) on skin function, we characterized the recovery of skin barrier function and the change in sphingolipid metabolism-related enzymes in the epidermis using a special Mg-deficient diet-induced atopic dermatitis-like skin and tape-stripping damaged skin murine models. Our results show that dietary GluCer and SM accelerate the recoveries of damaged skin barrier functions. Correspondingly, dietary sphingolipids significantly upregulated the expression of ceramide synthases 3 and 4 in the epidermis of the atopic dermatitis-like skin model (P < 0.05). In the case of cultured cells, the expression of ceramide synthases 2-4 in normal human foreskin keratinocytes was significantly upregulated by treatment with 0.001-0.1 μm sphingoid bases (sphinganine, sphingosine and trans-4,cis-8-sphingadienine) (P < 0.05). These results suggest that the effects of dietary sphingolipids might be due to the activation of ceramide synthesis in the skin, rather than the direct reutilization of dietary sphingolipids. Our findings provide a novel insight into the mechanisms of the skin barrier improving effect and a more comprehensive understanding of dietary sphingolipids. © 2012 John Wiley & Sons A/S.


Fukumitsu S.,Nippon Flour Mills Co. | Aida K.,Nippon Flour Mills Co. | Shimizu H.,Nippon Flour Mills Co. | Toyoda K.,Nippon Flour Mills Co.
Nutrition Research | Year: 2010

The effects of flaxseed lignan (secoisolariciresinol diglucoside [SDG]) intake on hypercholesterolemia and liver disease risk factors in moderately hypercholesterolemic men were investigated. In a previous study, we reported that SDG attenuates high-fat, diet-induced hypercholesterolemia in mice. Here, we report a double-blinded, randomized, and placebo-controlled study in moderately hypercholesterolemic men in which we investigated the hypothesis that oral administration of SDG (20 or 100 mg) would decrease the level of blood cholesterol and liver disease risk factors induced by hypercholesterolemia in humans. Thirty men with total cholesterol levels of 4.65 to 6.21 mmol/L (180-240 mg/dL) were randomly assigned to 3 groups; 2 groups received flaxseed lignan capsules (SDG, 20 or 100 mg/d) and the other received placebo capsules for 12 weeks. We found that, compared to the subjects who received placebo, those who received 100 mg of SDG exhibited a significant reduction in the ratio of low-density lipoprotein/high-density lipoprotein cholesterol at baseline (P < .05) and at week 12 (P < .05). In addition, in SDG-treated subjects, we also observed a significant percentage decrease in the levels of glutamic pyruvic transaminase and γ-glutamyl transpeptidase relative to the levels at baseline (P < .01) and a significant percentage decrease in the level of γ -glutamyl transpeptidase relative to the placebo-treated group (P < .05). These results suggest that daily administration of 100 mg SDG can be effective at reducing blood level of cholesterol and hepatic diseases risk in moderately hypercholesterolemic men. © 2010 Elsevier Inc.


Sugawara T.,Kyoto University | Aida K.,Nippon Flour Mills Co. | Duan J.,Kyoto University | Hirata T.,Kyoto University
Journal of Oleo Science | Year: 2010

Liquid chromatography-mass spectrometry is one of the most powerful methods for the identification and detection of chemical structures of lipids. In this study, we attempted to identify the chemical (maitake) and sea cucumber by liquid chromatography-ion trap mass spectrometry. For structural analysis of glucosylceramides, [M+H]+,[M+H-18]+ or [M+H-162]+ in the positive scan mode was used for MS/MS analysis to obtain product ion spectra. The typical signals which are characteristic for the sphingoid base moieties were observed while the isomers could not be distinguished. This method should be useful for the structural determination of diverse glucosylceramide molecular species. © 2010 by Japan Oil Chemists' Society.


Duan J.,Kyoto University | Sugawara T.,Kyoto University | Sakai S.,Kyoto University | Aida K.,Nippon Flour Mills Co. | Hirata T.,Kyoto University
Lipids | Year: 2011

Sphingolipids are constituents of cellular membranes and play important roles as second messengers mediating cell functions. As significant components in foods, sphingolipids have been proven to be critical for human health. Moreover, diverse metabolic intermediates of sphingolipids are known to play key roles both in proinflammatory and in anti-inflammatory effects. However, the effect of dietary sphingolipids on inflammation is a complicated field that needs to be further assessed. Our study evaluated the effects of orally administered maize glucosylceramide (GluCer), one of the most conventional dietary sphingolipids, on inflammation using the 2,4-dinitro-1-fluorobenzene- treated BALB/c murine model. Oral administration of GluCer inhibited ear swelling and leukocyte infiltration to the inflammatory site, suggesting that dietary GluCer has anti-inflammatory properties. ELISA analyses revealed that oral administration of GluCer for 6 days had not modified the Th1/Th2 balance, but significantly down-regulated the activation of TNF-α at the inflammatory site. Based on these results, the down-regulation of TNF-α by dietary GluCer may suppress vascular permeability and reduce the migration of inflammatory cells. Our findings increase understanding of the actions of dietary sphingolipids on the balance of the immune response. © 2011 AOCS.


Trademark
Nippon Flour Mills Company Ltd | Date: 2014-10-29

Pasta.


Trademark
Nippon Flour Mills Company Ltd | Date: 2013-04-30

Pasta, excluding wheat-free and gluten-free pasta.

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