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Zhang B.,University of Sichuan | Zhu F.,Ninth Peoples Hospital of Chongqing | Ma X.,University of Sichuan | Tian Y.,University of Sichuan | And 5 more authors.
Radiotherapy and Oncology | Year: 2014

Purpose A population-based matched-pair comparison was performed to compare the efficacy of stereotactic body radiotherapy (SBRT) versus surgery for early-stage non-small cell lung cancer (NSCLC).Methods All the eligible studies were searched by PubMed, Medline, Embase, and the Cochrane Library. The meta-analysis was performed to compare odds ratios (OR) for overall survival (OS), cancer-specific survival (CSS), disease-free survival (DFS), local control (LC), and distant control (DC).Conclusions This systematic review found a superior 3-year OS after surgery compared with SBRT, which supports the need to compare both treatments in large prospective, randomized, controlled clinical trials.Results Six studies containing 864 matched patients were included in the meta-analysis. The surgery was associated with a better long-term OS in patients with early-stage NSCLC. The pooled OR and 95% confidence interval (CI) for 1-year, 3-year OS were 1.31 [0.90, 1.91] and 1.82 [1.38, 2.40], respectively. However, the difference in 1-year and 3-year CSS, DFS, LC and DC was not significant. ©2014 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology.

Xiang J.,Ninth Peoples Hospital of Chongqing | Xiang J.,Chongqing Medical University | Leung A.W.,Chinese University of Hong Kong | Xu C.,Chinese University of Hong Kong
Journal of Ultrasound in Medicine | Year: 2014

Objectives-This study aimed to investigate the effect of ultrasound sonication in the presence of methylene blue on clonogenic survival and mitochondria of ovarian cancer cells.Methods-Human ovarian cancer HO-8910 cells, which were incubated with different concentrations of methylene blue for 1 hour, were exposed to an ultrasonic wave for 5 seconds with intensity of 0.46 W/cm2. Clonogenic survival of HO-8910 cells after ultrasound sonication was measured by a colony-forming unit assay. Mitochondrial structural changes were observed on transmission electron microscopy, and the mitochondrial membrane potential was evaluated by confocal laser-scanning microscopy with rhodamine 123 staining.Results-The colony-forming units of HO-8910 cells decreased considerably after ultrasound sonication in the presence of methylene blue. Transmission electron microscopy showed slightly enlarged mitochondria in the ultrasound-treated cells in the absence of methylene blue; however, seriously damaged mitochondria, even with almost complete disappearance of cristae, were found in the cells treated by ultrasound sonication in the presence of methylene blue. The mitochondrial membrane potential collapsed significantly when HO-8910 cells were treated by ultrasound sonication in the presence of methylene blue (P < .05).Conclusions-Ultrasound sonication in the presence of methylene blue markedly damaged mitochondrial structure and function and decreased clonogenic survival of HO-8910 cells. © 2014 by the American Institute of Ultrasound in Medicine.

Cheng D.,Southwest University | Han W.,Southwest University | Yang K.,Southwest University | Song Y.,Southwest University | And 2 more authors.
Talanta | Year: 2014

Gold nanoparticles (AuNPs) have been widely used to develop fluorescence resonance energy transfer (FRET) sensors to detect biological substances, environmental pollutants, and disease markers due to their superior quenching capacity to fluorescence signals. In this study, we report the one-step facile synthesis of fluorescein isothiocyanate-labeled hyaluronic acid (FITC-HA) functionalized fluorescent AuNPs based FRET nanoprobes (FITC-HA-AuNPs) via chemical reduction of HAuCl4 by using FITC-HA as both a reducing and stabilizing agent. Then the FITC-HA-AuNPs FRET nanoprobes were used to detect hyaluronidase (HAase), a new type of disease marker, based on the specific enzymatic degradation of HAase to HA. Compared with similar work, the FITC-HA-AuNPs nanoprobes were much easier to prepare and the detection sensitivity was also high for HAase to reach a detection limit of 0.63 U mL -1. More importantly, they also allowed for rapid HAase detection (within 3 h) even in complex biological specimens (urine specimens from patients with bladder cancer) with satisfactory accuracy (recovery efficiency in the range of 92.8-106.9% with RSD≤4.85%). Our studies suggested that such a novel design of FITC-HA-AuNPs FRET nanoprobes developed for sensitive, rapid and accurate detection of HAase had exciting potentials for clinical diagnosis of HAase-related diseases, such as bladder cancer. © 2014 Elsevier B.V.

Song E.,Southwest University | Cheng D.,Southwest University | Song Y.,Southwest University | Jiang M.,Ninth Peoples Hospital of Chongqing | And 2 more authors.
Biosensors and Bioelectronics | Year: 2013

Graphene oxide (GO) has been widely used to develop fluorescence resonance energy transfer (FRET) biosensors for tumor markers (e.g., matrix metalloproteinases, MMPs) due to its superior fluorescence quenching capacity and unique adsorption characteristics for biomolecules. In this study, fluorescein isothiocyanate-labeled peptide (Pep-FITC) was assembled onto the GO surface through covalent binding to construct a GO-Pep-FITC FRET sensor for sensitive, rapid, and accurate detection of MMP-2 in complex serum samples. Compared to similar GO-based FRET sensors fabricated through physical adsorption, the as prepared ones via covalent binding are significantly more stable under physiological conditions, enabling their detection of MMP-2 with high sensitivity (detection limit: 2.5. ng/mL). More importantly, it allows for rapid MMP-2 detection (within 3. h) even in complex biological samples with satisfactory accuracy and the relative standard deviation ≤7.03%. Our studies further suggest that such a platform developed here for sensitive, rapid, and accurate detection of biomarkers holds great promise for clinical diagnosis of protease-related diseases. © 2013 Elsevier B.V.

Zhang B.,University of Sichuan | Ma X.,University of Sichuan | Zhan W.,University of Sichuan | Zhu F.,Ninth Peoples Hospital of Chongqing | And 6 more authors.
Ultrasound in Medicine and Biology | Year: 2014

The goal of the study described here was to assess the performance of real-time elastography (RTE) in the detection of prostate cancers using a meta-analysis. A literature search of PubMed, Medline, Embase and the Cochrane Library was conducted. Published studies that evaluated the diagnostic performance of RTE in the diagnosis of prostate cancer and using the histopathology of the radical prostatectomy specimen as a reference standard were included. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and area under the curve were calculated to examine the accuracy of RTE. A total of seven studies that included 508 patients were analyzed. The pooled sensitivity and specificity for the diagnosis of prostate cancer by RTE were 0.72 (95% confidence interval: 0.70-0.74) and 0.76 (0.74-0.78), respectively. The summary diagnostic odds ratio was 12.59 (7.26-21.84), and the area under the curve was 0.841 (Q*=0.773). In conclusion, RTE imaging has high accuracy in the detection of prostate cancers using the histopathology of the radical prostatectomy specimen as the reference standard and may reduce the number of core biopsies in the future. © 2014 World Federation for Ultrasound in Medicine & Biology.

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