Ninth Peoples Hospital of Chongqing

of Chongqing, China

Ninth Peoples Hospital of Chongqing

of Chongqing, China

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Zhang B.,University of Sichuan | Zhu F.,Ninth Peoples Hospital of Chongqing | Ma X.,University of Sichuan | Tian Y.,University of Sichuan | And 5 more authors.
Radiotherapy and Oncology | Year: 2014

Purpose A population-based matched-pair comparison was performed to compare the efficacy of stereotactic body radiotherapy (SBRT) versus surgery for early-stage non-small cell lung cancer (NSCLC).Methods All the eligible studies were searched by PubMed, Medline, Embase, and the Cochrane Library. The meta-analysis was performed to compare odds ratios (OR) for overall survival (OS), cancer-specific survival (CSS), disease-free survival (DFS), local control (LC), and distant control (DC).Conclusions This systematic review found a superior 3-year OS after surgery compared with SBRT, which supports the need to compare both treatments in large prospective, randomized, controlled clinical trials.Results Six studies containing 864 matched patients were included in the meta-analysis. The surgery was associated with a better long-term OS in patients with early-stage NSCLC. The pooled OR and 95% confidence interval (CI) for 1-year, 3-year OS were 1.31 [0.90, 1.91] and 1.82 [1.38, 2.40], respectively. However, the difference in 1-year and 3-year CSS, DFS, LC and DC was not significant. ©2014 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology.


Zou L.-Q.,University of Sichuan | Zhang B.-L.,University of Sichuan | Chang Q.,Ninth Peoples Hospital of Chongqing | Zhu F.-P.,Ninth Peoples Hospital of Chongqing | And 3 more authors.
World Journal of Gastroenterology | Year: 2014

AIM: To compare transcatheter arterial chemoembolization (TACE) and 3D conformal radiotherapy (3D-CRT) with TACE monotherapy in hepatocellular carcinoma (HCC). METHODS: We searched all the eligible studies from the Cochrane Library, PubMed, Medline, Embase, and CNKI. The meta-analysis was performed to assess the survival benefit, tumor response, and the decline in ?-fetoprotein (AFP) level. According to the heterogeneity of the studies, pooled OR with 95%CI were calculated using the fixed-effects or random-effects model. An observed OR > 1 indicated that the addition of 3D-CRT to TACE offered survival benefits to patients that could be considered statistically significant. Statistical analyses were performed using Review Manager Software. RESULTS: Ten studies met the criteria to perform a meta-analysis including 908 HCC participants, with 400 patients in the TACE/3D-CRT combination group and 508 in the TACE alone group. TACE combined with 3DCRT significantly improved 1-, 2- and 3-year overall survival compared with TACE monotherapy (OR = 1.87, 95%CI: 1.37-2.55, P < 0.0001), (OR = 2.38, 95%CI: 1.78-3.17, P < 0.00001) and (OR = 2.97, 95%CI: 2.10-4.21, P < 0.00001). In addition, TACE plus 3DCRT was associated with a higher tumor response (complete remission and partial remission) (OR = 3.81; 95%CI: 2.70-5.37; P < 0.00001), and decline rates of AFP level (OR = 3.24, 95%CI: 2.09-5.02, P < 0.00001). CONCLUSION: This meta-analysis demonstrated that TACE combined with 3D-CRT was better than TACE monotherapy for patients with HCC, which needs to be confirmed by large multicenter trials © 2014 Baishideng Publishing Group Inc. All rights reserved.


Luo Y.-Z.,Ninth Peoples Hospital of Chongqing | Li T.-J.,Ninth Peoples Hospital of Chongqing
World Chinese Journal of Digestology | Year: 2015

Kupffer cells (KCs) are the largest group of tissue macrophages. KCs in the hepatic sinusoid contact with pathogens from circulation earliest. The quantity of KCs increases significantly in patients with viral hepatitis, suggesting that KCs play an important role in viral hepatitis related immunity. KCs can not only engulf virus particles, but also present antigens, secrete cytokines, and participate in a variety of inflammatory responses, immune tolerance and liver injury. In hepatitis B virus (HBV)/hepatitis C virus (HCV) infections, the diversity of KCs-related cytokines, the complexity of interactions of receptor signaling pathways as well as the duality of cytotoxic effect, indicate that KCs play a dual role in the immune response; the activation of KCs regulates the balance of inflammatory and anti-inflammatory mechanisms in viral hepatitis. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Xiang J.,Ninth Peoples Hospital of Chongqing | Xiang J.,Chongqing Medical University | Leung A.W.,Chinese University of Hong Kong | Xu C.,Chinese University of Hong Kong
Journal of Ultrasound in Medicine | Year: 2014

Objectives-This study aimed to investigate the effect of ultrasound sonication in the presence of methylene blue on clonogenic survival and mitochondria of ovarian cancer cells.Methods-Human ovarian cancer HO-8910 cells, which were incubated with different concentrations of methylene blue for 1 hour, were exposed to an ultrasonic wave for 5 seconds with intensity of 0.46 W/cm2. Clonogenic survival of HO-8910 cells after ultrasound sonication was measured by a colony-forming unit assay. Mitochondrial structural changes were observed on transmission electron microscopy, and the mitochondrial membrane potential was evaluated by confocal laser-scanning microscopy with rhodamine 123 staining.Results-The colony-forming units of HO-8910 cells decreased considerably after ultrasound sonication in the presence of methylene blue. Transmission electron microscopy showed slightly enlarged mitochondria in the ultrasound-treated cells in the absence of methylene blue; however, seriously damaged mitochondria, even with almost complete disappearance of cristae, were found in the cells treated by ultrasound sonication in the presence of methylene blue. The mitochondrial membrane potential collapsed significantly when HO-8910 cells were treated by ultrasound sonication in the presence of methylene blue (P < .05).Conclusions-Ultrasound sonication in the presence of methylene blue markedly damaged mitochondrial structure and function and decreased clonogenic survival of HO-8910 cells. © 2014 by the American Institute of Ultrasound in Medicine.


Cheng D.,Southwest University | Han W.,Southwest University | Yang K.,Southwest University | Song Y.,Southwest University | And 2 more authors.
Talanta | Year: 2014

Gold nanoparticles (AuNPs) have been widely used to develop fluorescence resonance energy transfer (FRET) sensors to detect biological substances, environmental pollutants, and disease markers due to their superior quenching capacity to fluorescence signals. In this study, we report the one-step facile synthesis of fluorescein isothiocyanate-labeled hyaluronic acid (FITC-HA) functionalized fluorescent AuNPs based FRET nanoprobes (FITC-HA-AuNPs) via chemical reduction of HAuCl4 by using FITC-HA as both a reducing and stabilizing agent. Then the FITC-HA-AuNPs FRET nanoprobes were used to detect hyaluronidase (HAase), a new type of disease marker, based on the specific enzymatic degradation of HAase to HA. Compared with similar work, the FITC-HA-AuNPs nanoprobes were much easier to prepare and the detection sensitivity was also high for HAase to reach a detection limit of 0.63 U mL -1. More importantly, they also allowed for rapid HAase detection (within 3 h) even in complex biological specimens (urine specimens from patients with bladder cancer) with satisfactory accuracy (recovery efficiency in the range of 92.8-106.9% with RSD≤4.85%). Our studies suggested that such a novel design of FITC-HA-AuNPs FRET nanoprobes developed for sensitive, rapid and accurate detection of HAase had exciting potentials for clinical diagnosis of HAase-related diseases, such as bladder cancer. © 2014 Elsevier B.V.


Song E.,Southwest University | Cheng D.,Southwest University | Song Y.,Southwest University | Jiang M.,Ninth Peoples Hospital of Chongqing | And 2 more authors.
Biosensors and Bioelectronics | Year: 2013

Graphene oxide (GO) has been widely used to develop fluorescence resonance energy transfer (FRET) biosensors for tumor markers (e.g., matrix metalloproteinases, MMPs) due to its superior fluorescence quenching capacity and unique adsorption characteristics for biomolecules. In this study, fluorescein isothiocyanate-labeled peptide (Pep-FITC) was assembled onto the GO surface through covalent binding to construct a GO-Pep-FITC FRET sensor for sensitive, rapid, and accurate detection of MMP-2 in complex serum samples. Compared to similar GO-based FRET sensors fabricated through physical adsorption, the as prepared ones via covalent binding are significantly more stable under physiological conditions, enabling their detection of MMP-2 with high sensitivity (detection limit: 2.5. ng/mL). More importantly, it allows for rapid MMP-2 detection (within 3. h) even in complex biological samples with satisfactory accuracy and the relative standard deviation ≤7.03%. Our studies further suggest that such a platform developed here for sensitive, rapid, and accurate detection of biomarkers holds great promise for clinical diagnosis of protease-related diseases. © 2013 Elsevier B.V.


Tian X.,Southwest University | Wang L.-Y.,Southwest University | Xia S.,Southwest University | Li Z.-B.,Southwest University | And 4 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012

Novel 2H-benzo[b][1,4]oxazin-3(4H)-ones have been synthesized by condensation, reduction, O-alkylation and Smiles rearrangement using 3-bromo-4-hydroxy benzaldehyde, anilines, and chloroacetyl chloride as starting materials. All the synthesized compounds have been characterized by 1H NMR, 13C NMR, and HRMS, and tested for the inhibitory ability on platelet aggregation. The results have shown that the ADP (adenosine 5′-diphosphate)-induced platelet aggregation was inhibited by 7a-g with the IC 50 value at 10.14-18.83 μmol/L. Compound 7a exhibited the most potent inhibitory effect (IC 50 = 10.14 μmol/L) among all the compounds, but less potent than the control drug ticlopidine (3.18 μmol/L) and aspirin (6.07 μmol/L). The preliminary structure-activity relationship (SAR) was initially investigated in the study. © 2011 Elsevier Ltd. All rights reserved.


Zhang B.,University of Sichuan | Ma X.,University of Sichuan | Zhan W.,University of Sichuan | Zhu F.,Ninth Peoples Hospital of Chongqing | And 6 more authors.
Ultrasound in Medicine and Biology | Year: 2014

The goal of the study described here was to assess the performance of real-time elastography (RTE) in the detection of prostate cancers using a meta-analysis. A literature search of PubMed, Medline, Embase and the Cochrane Library was conducted. Published studies that evaluated the diagnostic performance of RTE in the diagnosis of prostate cancer and using the histopathology of the radical prostatectomy specimen as a reference standard were included. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and area under the curve were calculated to examine the accuracy of RTE. A total of seven studies that included 508 patients were analyzed. The pooled sensitivity and specificity for the diagnosis of prostate cancer by RTE were 0.72 (95% confidence interval: 0.70-0.74) and 0.76 (0.74-0.78), respectively. The summary diagnostic odds ratio was 12.59 (7.26-21.84), and the area under the curve was 0.841 (Q*=0.773). In conclusion, RTE imaging has high accuracy in the detection of prostate cancers using the histopathology of the radical prostatectomy specimen as the reference standard and may reduce the number of core biopsies in the future. © 2014 World Federation for Ultrasound in Medicine & Biology.


Zhao X.-Y.,Southwest University | Yang S.,Southwest University | Chen Y.-R.,Southwest University | Li P.-C.,Southwest University | And 2 more authors.
PLoS ONE | Year: 2014

Background and Aims: Arsenic trioxide (As2O3), which used as an effective agent in the treatment of leukaemia and other solid tumors, is largely limited by its toxicity. QT prolongation, torsades de pointes and sudden heart death have been implicated in the cardiotoxicity of As 2O3. The present study was designed to explore whether the combination of As2O3 and resveratrol could generate a more powerful anti-cancer effect both in vitro and in vivo. Materials and Methods: MTT assay was performed to assess the proliferation of Hela, MCF-7 and NB4 cells. Isobolographic analysis was used to evaluate combination index values from cell viability data. The apoptosis and the cellular reactive oxygen species (ROS) level were assessed by fluorescent microscopy and flow cytometry separately in vitro. The effect of As2O3, alone and in combination with resveratrol on Hela tumor growth in an orthotopic nude mouse model was also investigated. The tumor volume and the immunohistochemical analysis of CD31, CD34 and VEGF were determined. Results: Resveratrol dramatically enhanced the anti-cancer effect induced by As2O 3 in vitro. In addition, isobolographic analysis further demonstrated that As2O3 and resveratrol generated a synergistic action. More apoptosis and ROS generation were observed in the combination treatment group. Similar synergistic effects were found in nude mice in vivo. The combination of As2O3 and resveratrol dramatically suppressed both tumor growth and angiogenesis in nude mice. Conclusions: Combining As2O3 with resveratrol would be a novel strategy to treat cancer in clinical practice. © 2014 Zhao et al.


PubMed | University of Sichuan and Ninth Peoples Hospital of Chongqing
Type: Journal Article | Journal: Journal of clinical ultrasound : JCU | Year: 2016

The use of contrast-enhanced sonography (CEUS) has yielded promising results in the differentiation of thyroid nodules. We conducted this meta-analysis to assess its performance in identifying and distinguishing between benign and malignant thyroid nodules.PubMed, Medline, Embase, and the Cochrane Library were searched for studies published through the end of December 2013. Sensitivity, specificity, positive and negative likelihood ratios, diagnostic odds ratio, and area under the curve were calculated.A total of 13 studies were included in this meta-analysis. For the diagnosis of malignant thyroid nodules worldwide, the overall mean rates of sensitivity and specificity of CEUS were 90% (95% confidence interval [CI], 88-93%) and 86% (95% CI, 83-89%), respectively. The summary diagnostic odds ratio was 52.83 (95% CI, 21.71-128.55), and the area under the curve for the summary receiver operating characteristic curve was 0.94 (95% CI, 0.90-0.98).This meta-analysis indicates that CEUS may be a valuable supplemental method, with high rates of sensitivity and specificity, to use for identifying and distinguishing between benign and malignant thyroid nodules.

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