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Yinchuan, China

Zhang H.,Ningxia Medical University | Liu J.,Wenzhou Medical College
Journal of Bioactive and Compatible Polymers | Year: 2013

Biocomposite scaffolds consist of poly(lactic-co-glycolic acid) and wool keratin were obtained by an electrospinning process. Scanning electron microscopy images showed that the poly(lactic-co-glycolic acid)/wool keratin fibers had relatively rougher surfaces and smaller diameters. Thermogravimetric analysis showed higher thermal stabilities of the developed biocomposites compared to neat poly(lactic-co-glycolic acid). Mechanical tests showed that when the wool keratin content increased from 0% to 0.5% w/v, the tensile strength and elongation at break of the poly(lactic-co-glycolic acid)/0.5% wool keratin scaffolds increased with maxima of 6.59 MPa and 104.44%, respectively, which was an increase of 8.2% and 570% over the poly(lactic-co-glycolic acid) scaffold. The biological response of bone mesenchymal stem cells to the poly(lactic-co-glycolic acid)/1.5% wool keratin biocomposites was superior when compared to pure poly(lactic-co-glycolic acid) scaffold in terms of improved cell attachment and higher proliferation. These observations suggest that the addition of wool keratin to a poly(lactic-co-glycolic acid) matrix can improve several properties of the electrospun poly(lactic-co-glycolic acid) fibers, and the poly(lactic-co-glycolic acid)/wool keratin biocomposites could make excellent materials for tissue engineering applications. © The Author(s) 2013. Source

Objective To comprehensively evaluate the relationship between murine double minute 2 (MDM2) gene promoter SISP309 T>G polymorphism and the susceptibility of gastrointestinal cancer. Methods The China National Knowledge Infrastructure (CNKI), WanFang database, SpringerLink database and PubMed were retrieved to get all case-control research literatures (2005-2012) on the relationship of MDM2 gene SNP309 T>G and gastrointestinal cancer susceptibility. Meta-analysis with RevMan 4.2 was used to combine OR values of the relationship between SNP309 T>G and gastrointestinal cancer susceptibility. A sensitivity analysis and tested publication bias were made with all selected literatures' data. Results A total of 17 domestic and foreign qualified papers were included in this study. Twenty case-control studies including 5 183 cases and 6 660 controls were identified for the present meta-analysis. A significant association was detected between the MDM2 SXP309 T>G polymorphism and gastrointestinal cancer risk. The meta-analysis showed that the combined odds ratio (OR) for GG genotype was 2.23 (95 % CI = 1.73-2.89, P < 0.01) compared with that for TG + TT genotypes. There was no statistical significance for the evaluation of publication bias. Conclusion The GG genotype of MDM2 SNP309 may increase gastrointestinal cancer risk in Asians. Source

Zhang H.,Ningxia Medical University
Journal of Bioactive and Compatible Polymers | Year: 2011

A series of poly (lactic-co-glycolic acid) (PLGA)/multiwalled carbon nanotubes (MWNTs) composite scaffolds was prepared by electrospinning for tissue engineering applications. The effect of MWNTs content on the structure and properties of composite scaffolds was characterized by scanning electron microscopy (SEM), X-ray diffractrometry (XRD), thermogravimetric analysis (TGA), and universal testing machine (UTM). The attachment ability and viability of rat bone marrow-derived mesenchymal stem cells (BMSCs) in the presence of the scaffolds were also investigated. Morphological characterization showed that the addition of different amounts of MWNTs increased the average fiber diameter; for instance, from 717 nm (neat PLGA) to 2193 nm at 1.0% MWNTs. Thermal characterization showed that the incorporation of MWNTs into the PLGA matrix increased the thermal stability of the composite scaffolds. The analysis of mechanical properties of the PLGA/MWNTs composites revealed great improvement over pure PLGA scaffold. The attachment and proliferation of BMSCs were significantly increased in the PLGA/MWNTs scaffolds compared with the PLGA control. Therefore, the PLGA/MWNTs composite scaffolds fabricated by electrospinning may be potentially useful in tissue engineering applications, particularly as scaffolds for bone tissue regeneration. © 2011 The Author(s). Source

Ma X.J.,Ningxia Medical University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2013

To investigate the association between primary knee osteoarthritis (OA) and single nucleotide polymorphism (SNP) (A668G) of leptin receptor gene (LEPR) in the Ningxia Hui population. A case-control association study has been adopted in this thesis. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis were performed to investigate the SNP of A668G site within LEPR from 148 patients with knee OA and 155 controls (asymptomatic and radiographically negative) with matched age and gender among Ningxia Hui population. In addition, genotypes of LEPR were verified by direct sequence analysis on PCR products. The result indicates that allele and genotype frequencies (P=0.024 and 0.008, respectively) in LEPR SNP A668G were significantly different in the knee OA patients group and control group, and in the knee OA patients group, the serum levels of leptin decreased significantly (P<0.001) and the serum levels of soluble leptin receptor increased significantly (P<0.001) compared with control group. Therefore, LEPR SNP A668G is associated with susceptibility to knee OA, which would be used as the genetic marker in predicting the risk of knee OA and would be one of the candidate genes in early prevention and control. Source

Sun P.,Ningxia Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To study the distribution of human papillomavirus (HPV) subtypes in nasal inverted papilloma (NIP), and to evaluate the relationship between HPV and NIP. Twenty-one HPV subtypes were detected in paraffin-embedded tissues of 101 cases of NIP by flow through hybridization and gene chip (HybriMax), 24 cases of normal nasal mucosa were used as controls. HPV positive rates of NIP were 64.36% (65/101). Benign NIP group, NIP with atypical hyperplasia group, NIP with cancerous group of HPV positive rates were 59.7% (46/77), 81.8% (18/22) and 50% (1/2) respectively. The control group was negative (0/24). The comparison between NIP group and control group was statistically significant (chi(2) = 32.178, P < 0.05). Benign NIP group and NIP with atypical hyperplasia group were compared, but no statistically significance (chi(2) = 3.649, P = 0.056) was found. The constituent ratio of benign NIP group and NIP with atypical hyperplasia group in high, low-risk HPV subtypes infections was compared, a statistically significance (chi(2) = 10.412, P < 0.05) was found. The occurrence of NIP was related with HPV infection. High-risk HPV subtype infections or multiple infections will prompt benign NIP to NIP with atypical hyperplasia. Understanding the distribution of HPV subtypes in the NIP is helpful to predict the clinical behavior. Source

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