Yinchuan, China

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Objective To comprehensively evaluate the relationship between murine double minute 2 (MDM2) gene promoter SISP309 T>G polymorphism and the susceptibility of gastrointestinal cancer. Methods The China National Knowledge Infrastructure (CNKI), WanFang database, SpringerLink database and PubMed were retrieved to get all case-control research literatures (2005-2012) on the relationship of MDM2 gene SNP309 T>G and gastrointestinal cancer susceptibility. Meta-analysis with RevMan 4.2 was used to combine OR values of the relationship between SNP309 T>G and gastrointestinal cancer susceptibility. A sensitivity analysis and tested publication bias were made with all selected literatures' data. Results A total of 17 domestic and foreign qualified papers were included in this study. Twenty case-control studies including 5 183 cases and 6 660 controls were identified for the present meta-analysis. A significant association was detected between the MDM2 SXP309 T>G polymorphism and gastrointestinal cancer risk. The meta-analysis showed that the combined odds ratio (OR) for GG genotype was 2.23 (95 % CI = 1.73-2.89, P < 0.01) compared with that for TG + TT genotypes. There was no statistical significance for the evaluation of publication bias. Conclusion The GG genotype of MDM2 SNP309 may increase gastrointestinal cancer risk in Asians.


Ma X.J.,Ningxia Medical University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2013

To investigate the association between primary knee osteoarthritis (OA) and single nucleotide polymorphism (SNP) (A668G) of leptin receptor gene (LEPR) in the Ningxia Hui population. A case-control association study has been adopted in this thesis. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis were performed to investigate the SNP of A668G site within LEPR from 148 patients with knee OA and 155 controls (asymptomatic and radiographically negative) with matched age and gender among Ningxia Hui population. In addition, genotypes of LEPR were verified by direct sequence analysis on PCR products. The result indicates that allele and genotype frequencies (P=0.024 and 0.008, respectively) in LEPR SNP A668G were significantly different in the knee OA patients group and control group, and in the knee OA patients group, the serum levels of leptin decreased significantly (P<0.001) and the serum levels of soluble leptin receptor increased significantly (P<0.001) compared with control group. Therefore, LEPR SNP A668G is associated with susceptibility to knee OA, which would be used as the genetic marker in predicting the risk of knee OA and would be one of the candidate genes in early prevention and control.


Wang C.,Ningxia Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To investigate the effect of high mobility group-box protein 1 (HMGB1)-siRNA on invasion and migration of human hepatoma cell line HepG2, and further to explore its mechanism. HMGB1-siRNA was synthesized with RNA interference and transferred into HepG2 cells to down-regulate the expression of HMGB1. The invasion and migration activities were assayed by Transwell™ assay and monolayer wounding healing assay. The levels of matrix metalloproteinase 2 (MMP-2), MMP-9, intercellular adhesion molecule 1 (ICAM-1) and tissue inhibitor of MMP-2 (TIMP-2) were detected by RT-PCR and Western blotting in HepG2 cells after treatment with 40 nmol/L HMGB1-siRNA for 24 h. The migration and invasion abilities of HepG2 cells were inhibited by 40 nmol/L HMGB1-siRNA markedly. Compared with the control group, MMP-2, MMP-9, ICAM-1 were down-regulated, TIMP-2 was up-regulated significantly by HMGB1-siRNA (P<0.05). HMGB1-siRNA can inhibit the invasion and migration abilities of human hepatoma cells by down-regulating the expressions of MMP-2, MMP-9, ICAM-1 and up-regulating the expression of TIMP-2.


Zhang H.,Ningxia Medical University
Journal of Bioactive and Compatible Polymers | Year: 2011

Poly(lactic-co-glycolic acid) (PLGA)/multiwalled carbon nanotubes (MWNTs) composite fiber mats were prepared by electrospinning and gas-jet/ electrospinning. The morphology and diameter of the electrospun PLGA/MWNTs fibers were tailored by controlling process parameters; smooth and uniform PLGA/MWNTs fibers were obtained by using the following optimized process parameters, 25 wt% PLGA concentrations, 0.25% w/v MWNTs contents, 20 kV applied voltage, 13 cm tip-to-collector distances, 0.27 mm inner diameters, and 0.2 mL/min feeding rates. The cytocompatibility of the PLGA/MWNTs fibers prepared by optimizing process parameters was evaluated using rat bone marrow-derived mesenchymal stem cells. Good-quality cell attachment and characteristic cell morphology were observed on the fibers. The PLGA/MWNTs fiber mats, fabricated by electrospinning, indicate excellent potential for bone tissue engineering applications, particularly as scaffolds for bone tissue regeneration. © SAGE Publications 2011.


Zhang H.,Ningxia Medical University
Journal of Bioactive and Compatible Polymers | Year: 2011

A series of poly (lactic-co-glycolic acid) (PLGA)/multiwalled carbon nanotubes (MWNTs) composite scaffolds was prepared by electrospinning for tissue engineering applications. The effect of MWNTs content on the structure and properties of composite scaffolds was characterized by scanning electron microscopy (SEM), X-ray diffractrometry (XRD), thermogravimetric analysis (TGA), and universal testing machine (UTM). The attachment ability and viability of rat bone marrow-derived mesenchymal stem cells (BMSCs) in the presence of the scaffolds were also investigated. Morphological characterization showed that the addition of different amounts of MWNTs increased the average fiber diameter; for instance, from 717 nm (neat PLGA) to 2193 nm at 1.0% MWNTs. Thermal characterization showed that the incorporation of MWNTs into the PLGA matrix increased the thermal stability of the composite scaffolds. The analysis of mechanical properties of the PLGA/MWNTs composites revealed great improvement over pure PLGA scaffold. The attachment and proliferation of BMSCs were significantly increased in the PLGA/MWNTs scaffolds compared with the PLGA control. Therefore, the PLGA/MWNTs composite scaffolds fabricated by electrospinning may be potentially useful in tissue engineering applications, particularly as scaffolds for bone tissue regeneration. © 2011 The Author(s).


Wang Y.-Y.,Ningxia Medical University | Zhe H.,Ningxia Medical University | Zhao R.,Ningxia Medical University
Molecular Cancer | Year: 2014

Solid cancer remains a major cause of death in the world. As limited treatment options are currently available to patients with solid cancer, novel preventive control and effective therapeutic approaches are considered to be reasonable and decisive measures to combat this disease. The plant-derived triterpenoids, commonly used for medicinal purposes in many Asian countries, poses various pharmacological properties. A large number of triterpenoids exhibit cytotoxicity against a variety of cancer cells, and cancer preventive, as well as anticancer efficacy in preclinical animal models. To improve antitumor activity, some synthetic triterpenoid derivatives have been synthesized, including cyano-3,12-dioxooleana-1,9(11)- dien-28-oic (CDDO), its methyl ester (CDDO-Me), and imidazolide (CDDO-Im) derivatives. In this review, we will critically examine the current preclinical evidences of cancer preventive and therapeutic activity about one of the synthetic triterpenoids, CDDO-Me. Both in vitro and in vivo effects of this agent and related molecular mechanisms are presented. © 2014 Wang et al.; licensee BioMed Central Ltd.


Zhao X.,Ningxia Medical University | Yang L.,Ningxia Medical University | Hu J.,Ningxia Medical University
Journal of Experimental and Clinical Cancer Research | Year: 2011

Aims: Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells. Methods: The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc. Results: Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21. Conclusions: MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy. © 2011 Zhao et al; licensee BioMed Central Ltd.


The present invention relates to a method for processing human placental cell sample, a human placental cell sample obtained according to said method for processing human placental cell sample, a human placental cell bank, a method for banking human placental cells, a method for searching human placental cell sample in said human placental cell bank according to the present invention, a method for preparing human cord blood serum, use of human placental cells obtained by said method for processing human placental cell sample or human placental cell bank established by said method for banking human placental cells in treating human dysfunction and diseases due to cell injury or cell malfunction, as well as a method for treating human dysfunction and diseases due to cell injury or cell malfunction.


Patent
Ningxia Medical University | Date: 2010-07-22

The present invention belongs to the field of cell transplantation. Particularly, the present invention provides a method of producing neurons for treating injuries with the loss of neuron function from stem cells, wherein the stem cell is a human derived mesenchymal stem cell, preferably human derived placental mesenchymal stem cell, bone marrow mesenchymal stem cell, adipose mesenchymal stem cell and liver mesenchymal stem cell. The present invention also provides the uses of said method and the nerve cells produced by said method in the preparation of medicines for treating injuries with the loss of neuron function and in the treatment of injuries with the loss of neuron function. The present invention further provides a method of treating injuries with the loss of neuron function.


Patent
Ningxia Medical University | Date: 2013-03-27

The present invention belongs to the field of cell transplantation. Particularly, the present invention provides a method of producing neurons for treating injuries with the loss of neuron function from stem cells, wherein the stem cell is a human derived mesenchymal stem cell, preferably human derived placental mesenchymal stem cell, bone marrow mesenchymal stem cell, adipose mesenchymal stem cell and liver mesenchymal stem cell. The present invention also provides the uses of said method and the nerve cells produced by said method in the preparation of medicines for treating injuries with the loss of neuron function and in the treatment of injuries with the loss of neuron function. The present invention further provides a method of treating injuries with the loss of neuron function.

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