Ningxia Key Laboratory of Clinical and Pathogenic Microbiology

Yinchuan, China

Ningxia Key Laboratory of Clinical and Pathogenic Microbiology

Yinchuan, China
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Duan X.,Ningxia Medical University | Duan X.,Ningxia Key Laboratory of Clinical and Pathogenic Microbiology | Bai J.,Ningxia Medical University | Wei J.,Ningxia Medical University | And 5 more authors.
Molecular Medicine Reports | Year: 2017

S-phase kinase-associated protein 2 (SKP2), a potent oncogene was revealed to be upregulated in gastric cancer (GC) tissue samples, in which SKP2 was inversely correlated with microRNA (miR)-508-5p transcripts. In present study, the functional effect of miR-508-5p on SKP2 and its metastatic potential were investigated in SGC-7901 GC cells. Significant downregulation of the miR-508-5p transcript was associated with the progression of GC. Furthermore, the overexpression of miR-508-5p was demonstrated to inhibit the proliferation, migration and invasion of SGC-7901 cells, as well as induced cell apoptosis and cell cycle arrest at the G0/G1 phase in vitro. The overexpression of miR-508-5p was able to downregulate the expression of the SKP2 oncogene, through a mechanism by which miR-508-5p directly targeted the SKP2 gene. Thus, regulating transcriptional and post-transcriptional SKP2 expression, as demonstrated using luciferase reporter assays, reverse transcription-quantitative polymerase chain reaction analysis and immunoblotting assays. The results of the present study identified that miR-508-5p functionally affects the SKP2 gene and reduces metastatic potential in GC, suggesting a novel role of miR-508-5p in the regulation of SKP2 and cell cycle.


Chu Y.,Ningxia Medical University | Fan W.,Ningxia Medical University | Guo W.,Ningxia Medical University | Zhang Y.,Ningxia Medical University | And 8 more authors.
Oncology Reports | Year: 2017

Increasing evidence suggests that aberrant expression of certain microRNAs (miRNAs) may participate in the genesis and progression of tumors. Several studies have indicated that miR-1247-5p plays different roles in various types of cancer cells. The effects of miR-1247-5p on human hepatocellular carcinoma (HCC) cells are elusive. In the present study, we investigated the effects of miR-1247-5p on the progression of HCC. The transcript of miR-1247-5p was markedly downregulated in clinical samples of patients with HCC and HCC cell lines, and ectopic overexpression of miR-1247-5p markedly inhibited the proliferation and invasion of HepG2 cells, induced cell apoptosis in vitro, and suppressed the growth of transplanted tumors in vivo. Wnt3 was found to be a potential target of miR-1247-5p and overexpression of miR-1247-5p was able to significantly downregulate the expression of Wnt3 by directly targeting the 3'UTR of this gene, which was verified by luciferase reporter assay and western blotting. Furthermore, we found that the miR-1247-5p gene was hypermethylated in HepG2 cells, and the transcript of miR-1247-5p was increased significantly after treatment with the demethylation drug 5-azacytidine. These findings demonstrated that miR-1247-5p functions as a tumor suppressor in human HCC by targeting Wnt3 and that the expression of miR-1247-5p can be regulated by DNA methylation, which indicates that miR-1247-5p has the potential to be a therapeutic target as well as a diagnostic marker of HCC.


Duan X.,Ningxia Medical University | Duan X.,Ningxia Key Laboratory of Clinical and Pathogenic Microbiology | Zhang T.,Ningxia Medical University | Zhang T.,Ningxia Key Laboratory of Clinical and Pathogenic Microbiology | And 11 more authors.
PLoS ONE | Year: 2015

To explore the potential roles of miRNAs in controlling the survival of mycobacteria in macrophages, miR-17-5p in the regulation of Bacillus Calmette-Guérin(BCG)growth in the macrophage RAW264.7 cells was interrogated. Our results reveal that an infection of BCG shows a time-dependent up-regulation of miR-17-5p in RAW264.7 cells in early phase; importantly, excessive expression of miR-17-5p in these cells exhibits an increased propagation of intracellular BCG. Mechanistically, the Unc-51 like autophagy activating kinase 1 (ULK1), an initial molecular of autophagy are identified as novel target of miR-17-5p, the miR-17-5p is capable of targeting down-regulating the expression of ULK1 protein. In addition, an overexpression of miR-17-5p in RAW264.7 cells is correlated with repression of ULK1 and the autophagosome related proteins LC3I/II. These results imply that miR-17-5p may be able to arrest the maturation of mycobacterial phagosomes in part by targeting ULK1, subsequently reduces the ability of host cells to kill intracellular BCG. © 2015 Duan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Jia W.,Ningxia Key laboratory of Clinical and Pathogenic Microbiology | Jia W.,Ningxia Medical University | Li C.,Ningxia Key laboratory of Clinical and Pathogenic Microbiology | Li C.,Ningxia Medical University | And 7 more authors.
International Journal of Environmental Research and Public Health | Year: 2015

The objective of this study was to explore the molecular epidemiology and the genetic support of clinical multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates in an ICU ward of a comprehensive hospital. A total of 102 non-duplicate drug-resistant A. baumannii isolates were identified and 93 (91.1%) of them were MDR strains. Molecular analysis demonstrated that carbapenemase genes blaOXA-23 and blaOXA-51 were presented in all 93 MDR isolates (100%), but other carbapenemase genes, including blaOXA-24, blaOXA-58, blaIMP-1, blaIMP-4, blaSIM, and blaVIM genes were completely absent in all isolates. In addition, genes of AdeABC efflux system were detected in 88.2% (90/102) isolates. Interestingly, an addition to efflux pump inhibitor, reserpine could significantly enhance the susceptibility of MDR isolates to moxifloxacin, cefotaxime, and imipenem (p < 0.01). Clonal relationship analysis further grouped these clinical drug-resistant isolates into nine clusters, and the MDR strains were mainly in clusters A, B, C, and D, which include 16, 13, 25, and 15 isolates, respectively. This study demonstrated that clinical isolates carrying carbapenemase-encoding genes blaOXA-23 and AdeABC efflux pump genes are the main prevalent MDR A. baumannii, and the coexpression of oxacillinase and efflux pump proteins are thus considered to be the important reason for the prevalence of this organism in the ICU of this hospital. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Ningxia Key laboratory of Clinical and Pathogenic Microbiology and Ningxia Medical University
Type: Journal Article | Journal: International journal of environmental research and public health | Year: 2015

The objective of this study was to explore the molecular epidemiology and the genetic support of clinical multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates in an ICU ward of a comprehensive hospital. A total of 102 non-duplicate drug-resistant A. baumannii isolates were identified and 93 (91.1%) of them were MDR strains. Molecular analysis demonstrated that carbapenemase genes blaOXA-23 and blaOXA-51 were presented in all 93 MDR isolates (100%), but other carbapenemase genes, including blaOXA-24, blaOXA-58, blaIMP-1, blaIMP-4, blaSIM, and blaVIM genes were completely absent in all isolates. In addition, genes of AdeABC efflux system were detected in 88.2% (90/102) isolates. Interestingly, an addition to efflux pump inhibitor, reserpine could significantly enhance the susceptibility of MDR isolates to moxifloxacin, cefotaxime, and imipenem (p < 0.01). Clonal relationship analysis further grouped these clinical drug-resistant isolates into nine clusters, and the MDR strains were mainly in clusters A, B, C, and D, which include 16, 13, 25, and 15 isolates, respectively. This study demonstrated that clinical isolates carrying carbapenemase-encoding genes blaOXA-23 and AdeABC efflux pump genes are the main prevalent MDR A. baumannii, and the co-expression of oxacillinase and efflux pump proteins are thus considered to be the important reason for the prevalence of this organism in the ICU of this hospital.

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