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Fan Z.-Z.,Ningxia Medical University | Zhao W.-H.,Ningxia Medical University | Guo J.,Ningxia Medical University | Cheng R.-F.,Ningxia Medical University | And 5 more authors.
Yaoxue Xuebao

Adult rats chronic unpredictable stress model of depression (CUS) was adopted to elucidate the antidepressant pharmacological activity and related neurogenesis protective effect of the total flavonoids extract (licorice flavonoids, LF) from the Glycyrrhiza uralensis Fisch. cultivated locally in Ningxia. The rats were exposed to 9 kinds of unpredictable sequence of stressors and were given flavonoids (300 mg·kg-1, 100 mg·kg -1 and 30 mg·kg-1) for 28 days. The antidepressant effect was elucidated by open field test, forced swimming test and tail suspension test. The level of serum corticosterone was detected by radioimmunoassay. 5′-Bromo-2′-deoxyuridine (BrdU) labeling experiments was employed to study the neurogenesis protective activities. The flavonoids can increase the sum of line crosses and number of rears, and decrease the number of fecal boli produced in the open field test of the CUS rats. Also the flavonoids can decrease the immobility time in forced swim test as well as in the tail suspension test. In addition, the flavonoids (300 mg·kg-1) can decrease the serum corticosterone level of the CUS rats, and increase the number of the new born BrdU positive progenitor cells at the subgranular zone (SGZ) of dentate gyrus (DG) region in hippocampus. The results demonstrated that the total flavonoids extract from the cultivated Glycyrrhiza uralensis Fisch. could produce the antidepressive effect on chronic unpredictable stress of depression model rats and its mechanism may be associated with its neurogenesis protective effect. Source

Hua B.,Ningxia Medical University | Cheng R.-F.,Ningxia Medical University | Jing J.,Ningxia Medical University | Xue M.-Q.,Ningxia Medical University | And 7 more authors.
Chinese Journal of New Drugs

Objective: To investigate the antidepressant effect and related mechanism of the licorice flavonoids (LF) from the Glycyrrhiza uralensis. Methods: Male Sprague-Dawley rats (n=60) were randomly divided into 5 groups. The rats were exposed to 9 kinds of sequent stressors in an unpredictable order and at an unpredictable time of the day for 28 day. Fluoxetine (10 mg·kg-1) or flavonoids (100 and 300 mg·kg-1) were intragastrically administrated once a day accordingly. The forced swimming test (FST), tail suspension test (TST) and open field test (OFT) were employed to elucidate the antidepressant effects. The protein expression levels of synaptophysin (SYP) and postsynaptic density-95 (PSD-95) in the hippocampus were measured by immunocytochemistry and Western-blotting analysis, and the mRNA expression levels of SYP and PSD-95 were analyzed by qRT-PCR, respectively. Results: The flavonoids showed an antidepressant effect in rat CUS model. The flavonoids significantly up-regulated the protein and mRNA expression levels of SYP and down-regulated the protein and mRNA expression levels of PSD-95 in the hippocampus. Conclusion: The antidepressant effect of the licorice flavonoids from Glycyrrhiza uralensis may relate to modulate the expression of the critical proteins associated with synaptic structural and plasticity in the hippocampus. Source

Jtng J.,Ningxia Medical University | Zhao J.-Y.,Ningxia Medical University | Hua B.,Ningxia Medical University | Xue M.-Q.,Ningxia Medical University | And 4 more authors.
Zhongguo Zhongyao Zazhi

Objective: To study the inhibitory effect of flavonoids from Glycyrrhiza uralensis on thioacetamide-induced chonic hepatic fibrosis in rats and the effect on the protein expressions of transforming growth factor-β1 (TGF-β1) and Caspase-3 in livers. Method: Male Sprague-Dawley rats were randomly divided into totally seven groups; the normal control group, the model group, LF groups s (400, 200, 100, 50 mg · kg-1 · d-1) and the silymarin positive control group (30 mg · kg-1 · d-1). The hepatic fibrosis model was induced in the rats through intraperitoneal injection with 3% thioacetamide (TAA) at a dose of 150 mg · kg-1 body weight twice a week for 12 weeks. During the course, the control group and the model group were orally administered with saline (1 mL · kg-1 · d-1). After the modeling and drug intervention, the pathologic changes and fibrosis in liver tissues were observed by HE staining and Masson s Trichrome staining. The serum alanine transaminase (ALT) , aspartate transaminase (AST) , alkaline phosphatase (ALP) and liver hydroxyproline (HYP) contents were assayed by biochemical process. The serum hyaluronic acid (HA) was assessed by radioimmunoassay. In addition, the protein expressions of liver TGF-β1 and Caspase-3 were examined by immunohistochemical method. The mRNA expression of TGF-β1 in hepatic tissues was examined by quantitative Real-time PCR analysis. Result; Compared with the model group, flavonoids can protect the integrity of the structure of liver tissues, significantly reduce the hepatic cell degeneration and necrosis and the proliferation of fibrous tissues, notably reduce the serum AST, ALT, ALP and HA and IIYP in hepatic tissues and down-regulate the protein expressions of liver TGF-β1 and Caspase-3 and the mRNA expression of TGF-β1, in hepatic tissues. Conclusion; The licorice flavonoids (ran resist the thioacetamide-induced hepatic fibrosis in rats. Its mechanism may be related to the down- regulation of the protein expressions of TGF-β1, and Caspase-3. Source

Li W.,Toho University | Li S.,Toho University | Lin L.,Toho University | Lin L.,Heilongjiang Provincial Institute for Drug Control | And 6 more authors.
Natural Product Communications

The EtOAc extract of the roots of Glycyrrhiza uralensis exhibited α-glucosidase inhibitory activity. Bioassay-guided fractionation resulted in the isolation of an active prenylflavonoid, glycyrrhisoflavone. Its structure was elucidated by NMR and MS analyses. A simple method to prepare glycyrrhisoflavone from the 95% EtOH extract of the roots of G. uralensis was developed by combination of Diaion HP-20 column chromatography (CC), silica gel CC, and preparative HPLC. An HPLC-PDA method was developed for quantitative determination of glycyrrhisoflavone in the roots of G. uralensis. The sample was extracted with MeOH and analyzed using a reversed-phase column with isocratic elution with CH3CN-H2O (0.06% trifluoroacetic acid) (42:58) at a flow rate of 1.2 mL/min, a column temperature of 40°C, and a detection wavelength of 260 nm. The method allowed the determination of glycyrrhisoflavone in the concentration range of 5-500 μg/mL. The relative standard deviation values of the precision and repeatability were 0.3% and 2.0%, respectively. The limits of detection and quantification were 0.5 μg/mL and 5 μg/mL, respectively. The relative recovery rate was 100.2 ± 1.8%. Based on the validation results, the HPLC determination method was found to be precise, accurate, and time conservative. This method was applied successfully to nine different root samples of G. uralensis. The amounts of glycyrrhisoflavone in these samples were 15-93 mg/100 g of dried powdered plant material. Source

Kato H.,Toho University | Li W.,Toho University | Koike M.,Toho University | Wang Y.,Ningxia Institute for Drug Control | Koike K.,Toho University

Phytochemical investigation of the methanolic extract from the aerial parts of Agrimonia pilosa led to the isolation of three compounds, (-)-aromadendrin 3-O-β-d-glucopyranoside (1), desmethylagrimonolide 6-O-β-d- glucopyranoside (2), and 5,7-dihydroxy-2-propylchromone 7-O-β-d- glucopyranoside (3), together with nine known compounds, agrimonolide 6-O-glucoside, takanechromone C, astragalin, afzelin, tiliroside, luteolin, quercetin, isoquercetrin, and quercitrin. Their structures were determined by various spectroscopic analysis and chemical transformations. © 2010 Elsevier Ltd. All rights reserved. Source

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