Ningxia Institute for Drug Control

Yinchuan, China

Ningxia Institute for Drug Control

Yinchuan, China
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Ma L.,Toho University | Ma L.,Ningxia Institute for Drug Control | Li W.,Toho University | Wang H.,Ningxia Medical University | And 5 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2015

Ardisia plant species have been used in traditional medicines, and their bioactive constituents of 13,28-epoxy triterpenoid saponins have excellent biological activities for new drug development. In this study, a fast and simple method based on ultrafast liquid chromatography coupled to electrospray ionization mass spectrometry (UFLC-MS) was developed to simultaneously identify and quantitatively analyze triterpenoid saponins in Ardisia crenata extracts. In total, 22 triterpenoid saponins, including two new compounds, were identified from A. crenata. The method exhibited good linearity, precision and recovery for the quantitative analysis of eight marker saponins. A relative quantitative method was also developed using one major saponin (ardisiacrispin B) as the standard to break through the choke-point of the lack of standards in phytochemical analysis. The method was successfully applied to quantitatively analyze saponins in commercially available plant samples. This study describes the first systematic analysis of 13,28-epoxy-oleanane-type triterpenoid saponins in the genus Ardisia using LC-ESI-MS. The results can provide the chemical support for further biological studies, phytochemotaxonomical studies and quality control of triterpenoid saponins in medicinal plants of the genus Ardisia. © 2014 Elsevier B.V.


Fan Z.-Z.,Ningxia Medical University | Zhao W.-H.,Ningxia Medical University | Guo J.,Ningxia Medical University | Cheng R.-F.,Ningxia Medical University | And 5 more authors.
Yaoxue Xuebao | Year: 2012

Adult rats chronic unpredictable stress model of depression (CUS) was adopted to elucidate the antidepressant pharmacological activity and related neurogenesis protective effect of the total flavonoids extract (licorice flavonoids, LF) from the Glycyrrhiza uralensis Fisch. cultivated locally in Ningxia. The rats were exposed to 9 kinds of unpredictable sequence of stressors and were given flavonoids (300 mg·kg-1, 100 mg·kg -1 and 30 mg·kg-1) for 28 days. The antidepressant effect was elucidated by open field test, forced swimming test and tail suspension test. The level of serum corticosterone was detected by radioimmunoassay. 5′-Bromo-2′-deoxyuridine (BrdU) labeling experiments was employed to study the neurogenesis protective activities. The flavonoids can increase the sum of line crosses and number of rears, and decrease the number of fecal boli produced in the open field test of the CUS rats. Also the flavonoids can decrease the immobility time in forced swim test as well as in the tail suspension test. In addition, the flavonoids (300 mg·kg-1) can decrease the serum corticosterone level of the CUS rats, and increase the number of the new born BrdU positive progenitor cells at the subgranular zone (SGZ) of dentate gyrus (DG) region in hippocampus. The results demonstrated that the total flavonoids extract from the cultivated Glycyrrhiza uralensis Fisch. could produce the antidepressive effect on chronic unpredictable stress of depression model rats and its mechanism may be associated with its neurogenesis protective effect.


Wang H.-Q.,Ningxia Medical University | Ma L.,Ningxia Institute for Drug Control | Wang Q.,Ningxia Institute for Drug Control | Dong L.,Ningxia Medical University | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016

The distribution information of Lycii Fructus was collected by interview investigation and field survey, and 46 related environmental factors were coUected, some kinds of functional chemical constituents the of Lycii Fructus were analyzed. Integrated climate, topography and other related ecological factors, the habitat suitability study was conducted based on Arc geographic information system ( ArcGIS), and maximum entropy model. The AUC of ROC curve was both above 0. 95, indicating that the predictive results with the maximum model were highly precise. The results showed that 5 major ecological factors had obvious influence on ecology suitability distributions of Lycii Fructus, including soil pH, soil subclaas, vegetation type and in August the average temperature et al. It is suitable for the living habits of the Lycii Fructus, dry, cool weather, more hardy, drought-resistant, alkali soil, which is suitable for distribution in the northern temperate plains. In addition, the ecological suitability regionalization based on the chemical constituents of Lycii Fructus also provides a new suitable distribution area other than the traditional distribution area, which provides a scientific basis for the reasonable introduction of Lycii Fructus.


Jtng J.,Ningxia Medical University | Zhao J.-Y.,Ningxia Medical University | Hua B.,Ningxia Medical University | Xue M.-Q.,Ningxia Medical University | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2015

Objective: To study the inhibitory effect of flavonoids from Glycyrrhiza uralensis on thioacetamide-induced chonic hepatic fibrosis in rats and the effect on the protein expressions of transforming growth factor-β1 (TGF-β1) and Caspase-3 in livers. Method: Male Sprague-Dawley rats were randomly divided into totally seven groups; the normal control group, the model group, LF groups s (400, 200, 100, 50 mg · kg-1 · d-1) and the silymarin positive control group (30 mg · kg-1 · d-1). The hepatic fibrosis model was induced in the rats through intraperitoneal injection with 3% thioacetamide (TAA) at a dose of 150 mg · kg-1 body weight twice a week for 12 weeks. During the course, the control group and the model group were orally administered with saline (1 mL · kg-1 · d-1). After the modeling and drug intervention, the pathologic changes and fibrosis in liver tissues were observed by HE staining and Masson s Trichrome staining. The serum alanine transaminase (ALT) , aspartate transaminase (AST) , alkaline phosphatase (ALP) and liver hydroxyproline (HYP) contents were assayed by biochemical process. The serum hyaluronic acid (HA) was assessed by radioimmunoassay. In addition, the protein expressions of liver TGF-β1 and Caspase-3 were examined by immunohistochemical method. The mRNA expression of TGF-β1 in hepatic tissues was examined by quantitative Real-time PCR analysis. Result; Compared with the model group, flavonoids can protect the integrity of the structure of liver tissues, significantly reduce the hepatic cell degeneration and necrosis and the proliferation of fibrous tissues, notably reduce the serum AST, ALT, ALP and HA and IIYP in hepatic tissues and down-regulate the protein expressions of liver TGF-β1 and Caspase-3 and the mRNA expression of TGF-β1, in hepatic tissues. Conclusion; The licorice flavonoids (ran resist the thioacetamide-induced hepatic fibrosis in rats. Its mechanism may be related to the down- regulation of the protein expressions of TGF-β1, and Caspase-3.


Wang H.-Q.,Ningxia Medical University | Ma L.,Ningxia Institute for Drug Control | Wang Q.,Ningxia Institute for Drug Control | Dong L.,Ningxia Medical University | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016

The distribution information of Qycyrrhiza uralensis was collected by interview investigation and field survey, and 46 related environmental factors were collected, some kinds al. functional chemical constituents al. G. uralensis were analyzed Integrated climate, topography and other related ecological factors, the habitat suitability study was conducted based on Arc geographic information system( ArcGIS), and maximum entropy model. Ihe AUC of ROC curve was both above 0.95, indicating that the predictive results with the maximum model were highly precise. Ihe results showed that 5 major ecological factors have obvious influence on ecology suitability distributions al. G. uralensis, including July average temperature, soil sub category, Dec precipitation, vegetation types and standard deviation of seasonal variation in temperature, et al. It is suitable lor the living habits al. the G. uralensis, adequate light, low rainfall, summer heat and laig;e temperature difference between day and night, which is suitable lor distribution in the northern temperate plains and mountains. In addition, the ecological suitability regionalization based on the el cmical constituents al.G. uralensis also provides a new suitable distribution area other than the traditional distribution area, which provides a scientific basis lor the reasonable introduction al.G. uralensis.


Li W.,Toho University | Li S.,Toho University | Lin L.,Toho University | Lin L.,Heilongjiang Provincial Institute for Drug Control | And 6 more authors.
Natural Product Communications | Year: 2010

The EtOAc extract of the roots of Glycyrrhiza uralensis exhibited α-glucosidase inhibitory activity. Bioassay-guided fractionation resulted in the isolation of an active prenylflavonoid, glycyrrhisoflavone. Its structure was elucidated by NMR and MS analyses. A simple method to prepare glycyrrhisoflavone from the 95% EtOH extract of the roots of G. uralensis was developed by combination of Diaion HP-20 column chromatography (CC), silica gel CC, and preparative HPLC. An HPLC-PDA method was developed for quantitative determination of glycyrrhisoflavone in the roots of G. uralensis. The sample was extracted with MeOH and analyzed using a reversed-phase column with isocratic elution with CH3CN-H2O (0.06% trifluoroacetic acid) (42:58) at a flow rate of 1.2 mL/min, a column temperature of 40°C, and a detection wavelength of 260 nm. The method allowed the determination of glycyrrhisoflavone in the concentration range of 5-500 μg/mL. The relative standard deviation values of the precision and repeatability were 0.3% and 2.0%, respectively. The limits of detection and quantification were 0.5 μg/mL and 5 μg/mL, respectively. The relative recovery rate was 100.2 ± 1.8%. Based on the validation results, the HPLC determination method was found to be precise, accurate, and time conservative. This method was applied successfully to nine different root samples of G. uralensis. The amounts of glycyrrhisoflavone in these samples were 15-93 mg/100 g of dried powdered plant material.


Chen P.,Toho University | Chen P.,Ningxia Institute for Drug Control | Li W.,Toho University | Li Q.,Shenyang University | And 4 more authors.
Talanta | Year: 2011

A simple hydrophilic-interaction chromatography (HILIC) method was developed for the identification and quantification of 14 nucleosides and nucleobases, namely cytosine, uracil, cytidine, guanine, hypoxanthine, xanthine, uridine, thymine, inosine, guanosine, thymidine, 2′-deoxyadenosine, 2′-deoxyinosine and 2′-deoxyuridine in two traditional Chinese medicines, Geosaurus and Leech. The separation was achieved on a TSKgel Amide-80 column (150 mm × 2.0 mm, 3.0 μm) with a mixture of acetonitrile and 10 mM aqueous ammonium acetate as the mobile phase at a flow rate of 0.2 mL/min. The temperature was set at 30 °C and UV detection wavelength was set at 260 nm. All calibration curves showed good linearity (R 2 > 0.9957) within the test ranges. The overall intra- and inter-day RSD ranged from 0.4 to 3.4% and from 0.7 to 3.3%, respectively. The LOD and LOQ were in the range of 0.07-30.49 ng/mL and 0.26-60.98 ng/mL, respectively. The repeatability of the method was in the range of 2.2-5.8% for Geosaurus and 1.4-5.5% for Leech. The recoveries of the samples were in the range of 91.4-100.9% for Geosaurus, and 91.9-99.3% for Leech. The established method was applied successfully for the analysis of nucleosides and nucleobases in 22 commercially available samples collected from different regions in China and Japan. Our data showed that HILIC had advantages as a useful tool for the study of the bioactive components in Geosaurus and Leech as well as their quality control, and could therefore be used for the determination of the analytes in pharmaceutical products and biological fluids. © 2011 Elsevier B.V. All rights reserved.


Kato H.,Toho University | Li W.,Toho University | Koike M.,Toho University | Wang Y.,Ningxia Institute for Drug Control | Koike K.,Toho University
Phytochemistry | Year: 2010

Phytochemical investigation of the methanolic extract from the aerial parts of Agrimonia pilosa led to the isolation of three compounds, (-)-aromadendrin 3-O-β-d-glucopyranoside (1), desmethylagrimonolide 6-O-β-d- glucopyranoside (2), and 5,7-dihydroxy-2-propylchromone 7-O-β-d- glucopyranoside (3), together with nine known compounds, agrimonolide 6-O-glucoside, takanechromone C, astragalin, afzelin, tiliroside, luteolin, quercetin, isoquercetrin, and quercitrin. Their structures were determined by various spectroscopic analysis and chemical transformations. © 2010 Elsevier Ltd. All rights reserved.


Li S.,Toho University | Li W.,Toho University | Wang Y.,Ningxia Institute for Drug Control | Asada Y.,Tokyo University of Science | Koike K.,Toho University
Bioorganic and Medicinal Chemistry Letters | Year: 2010

Two new 2-arylbenzofurans, glycybenzofuran (1) and cyclolicocoumarone (2), together with 10 known flavonoids including licocoumarone (3), glycyrrhisoflavone (4), glisoflavone (5), cycloglycyrrhisoflavone (6), isoliquiritigenin (7), licoflavone A (8), apigenin (9), isokaempferide (10), glycycoumarin (11), and isoglycycoumarin (12), were isolated from the roots of Glycyrrhiza uralensis and their structures were determined by extensive spectroscopic analyses. Compounds 1 and 5 showed significant protein tyrosine phosphatase-1B (PTP1B) inhibitory activity in vitro with the IC50 values of 25.5 and 27.9 μM, respectively. The structure-activity relationship indicated that the presence of prenyl group and ortho-hydroxy group is important for exhibiting the activity. Kinetic analysis indicated that compound 1 inhibits PTP1B by a competitive mode, whereas compound 5 by a mixed mode. © 2010 Elsevier Ltd. All rights reserved.


PubMed | Ningxia Medical University, Ningxia Institute for Drug Control, Toho University, Shenyang University and Chiba Institute of Science
Type: | Journal: Journal of pharmaceutical and biomedical analysis | Year: 2015

Ardisia plant species have been used in traditional medicines, and their bioactive constituents of 13,28-epoxy triterpenoid saponins have excellent biological activities for new drug development. In this study, a fast and simple method based on ultrafast liquid chromatography coupled to electrospray ionization mass spectrometry (UFLC-MS) was developed to simultaneously identify and quantitatively analyze triterpenoid saponins in Ardisia crenata extracts. In total, 22 triterpenoid saponins, including two new compounds, were identified from A. crenata. The method exhibited good linearity, precision and recovery for the quantitative analysis of eight marker saponins. A relative quantitative method was also developed using one major saponin (ardisiacrispin B) as the standard to break through the choke-point of the lack of standards in phytochemical analysis. The method was successfully applied to quantitatively analyze saponins in commercially available plant samples. This study describes the first systematic analysis of 13,28-epoxy-oleanane-type triterpenoid saponins in the genus Ardisia using LC-ESI-MS. The results can provide the chemical support for further biological studies, phytochemotaxonomical studies and quality control of triterpenoid saponins in medicinal plants of the genus Ardisia.

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