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Ningbo, China

Zeng L.,Ningbo Institute of Materials Technology and Engineering | Ren W.,Ningbo Institute of Materials Technology and Engineering | Zheng J.,Ningbo No.2 Hospital | Cui P.,Ningbo Institute of Materials Technology and Engineering | Wu A.,Ningbo Institute of Materials Technology and Engineering
Physical Chemistry Chemical Physics

Using an improved hydrolysis method of inorganic salts assisted with water-bath incubation, ultrasmall water-soluble metal-iron oxide nanoparticles (including Fe 3O 4, ZnFe 2O 4 and NiFe 2O 4 nanoparticles) were synthesized in aqueous solutions, which were used as T 1-weighted contrast agents for magnetic resonance imaging (MRI). The morphology, structure, MRI relaxation properties and cytotoxicity of the as-prepared metal-iron oxide nanoparticles were characterized, respectively. The results showed that the average sizes of nanoparticles were about 4 nm, 4 nm and 5 nm for Fe 3O 4, ZnFe 2O 4 and NiFe 2O 4 nanoparticles, respectively. Moreover, the nanoparticles have good water dispersibility and low cytotoxicity. The MRI test showed the strong T 1-weighted, but the weak T 2-weighted MRI performance of metal-iron oxide nanoparticles. The high T 1-weighted MRI performance can be attributed to the ultrasmall size of metal-iron oxide nanoparticles. Therefore, the as-prepared metal-iron oxide nanoparticles with good water dispersibility and ultrasmall size can have potential applications as T 1-weighted contrast agent materials for MRI. © the Owner Societies 2012. Source

OBJECTIVE: To compare the effects of volume-controlled ventilation (VCV) and pressure-controlled volume-guaranteed (PCV-VG) mode during one-lung ventilation (OLV) on circulation, pulmonary function and lung injury.METHODS: 2012 February to 2013 March in Ningbo No2. Hospital cardiothoracic surgery, 30 patients aged 52 to 76 years (ASA grade II-III) undergoing elective thoracoscopic lobectomy were randomly divided into VCV group and PCV-VG group, with 15 cases in each group. After anesthesia induction and endotracheal intubation, endobronchial blocker was inserted to start OLV. Heart rate (HR), mean arterial pressure (MAP), measured tidal volume (TV), peak airway pressure (Ppeak), airway resistance (Raw), chest compliance (Cdyn) and the end-tidal carbon dioxide pressure (PetCO(2)) were recorded at the time point of 15 minutes after turning to the lateral position, 15 minutes and 60 minutes after OLV, and 15 minutes after the resumption of two lung ventilation. In the meanwhile, arterial blood gas analysis was conducted to measure indicators of pH, oxygen tension (PaO(2)) and carbon dioxide partial pressure (PaCO(2)). Blood was drawn before induction, 1 hour after OLV and 1 hour after the end of surgery, and the concentration of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA).RESULTS: HR, MAP, TV, PetCO(2), pH and PaCO(2) in two groups at the time point of 15 minutes after turning to the lateral position, 15 minutes and 60 minutes after OLV, and 15 minutes after the resumption of two lung ventilation showed no significant difference (P > 0.05). At the point of 15 minutes after turning to the lateral position and 15 minutes after two lung ventilation, Ppeak and Cdyn of two groups were significantly different (P < 0.05) (Ppeak:16 ± 3 cmH(2)O, 16 ± 3 cmH(2)O for VCV group and 14 ± 2 cmH(2)O, 14 ± 2 cmH(2)O for PCV-VG group; Cdyn: 43.5 ± 5.9 ml/cmH(2)O, 43.8 ± 6.7 ml/cmH2O for VCV group and 49.7 ± 7.1 ml/cmH(2)O, 53.3 ± 9.6 ml/cmH(2)O for PCV-VG group). Compared with VCV group, PCV-VG group showed a lower Ppeak 15 minutes and 60 minutes after OLV [ (17 ± 2 cmH(2)O) vs (22 ± 4 cmH(2)O) and (18 ± 4 cmH(2)O) vs( 23 ± 3 cmH(2)O) with a higher Cdyn at the same point (38.6 ± 6.3 ml/cmH(2)O) vs (29.6 ± 3.2 ml/cmH(2)O) and 37.3 ± 6.0 ml/cmH(2)O) vs (30.3 ± 3.8 ml/cmH(2)O)] (P < 0.01). Compared with VCV group,IL-6 and TNF-α of PCV-VG group 1 hour after OLV and 1 hour after the end of surgery were significantly lower (P < 0.01) (IL-6: 52.32 ± 3.59 vs 62.65 ± 4.17 pg/ml and 63.57 ± 4.98 vs 82.38 ± 4.10 pg/ml; TNF-α: 3.23 ± 0.27 vs 4.19 ± 0.38 pg/ml and 4.01 ± 0.28 vs. 5.49 ± 0.31 pg/ml).CONCLUSION: During one-lung ventilation in thoracoscopic lobectomy, PCV-VG mode has a competitive advantage over VCV mode in terms of pulmonary function and lung protection. Source

Zou X.,Shanghai JiaoTong University | Zhang G.,Shanghai JiaoTong University | Cheng Z.,Ningbo No.2 Hospital | Yin D.,Shanghai Key Laboratory of Tissue Engineering | And 6 more authors.
Stem Cell Research and Therapy

Introduction. Studies have demonstrated that mesenchymal stromal cells (MSCs) could reverse acute and chronic kidney injury by a paracrine or endocrine mechanism, and microvesicles (MVs) have been regarded as a crucial means of intercellular communication. In the current study, we focused on the therapeutic effects of human Wharton-Jelly MSCs derived microvesicles (hWJMSC-MVs) in renal ischemia/reperfusion injury and its potential mechanisms. Methods. MVs isolated from conditioned medium were injected intravenously in rats immediately after ischemia of the left kidney for 60 minutes. The animals were sacrificed at 24 hours, 48 hours and 2 weeks after reperfusion. The infiltration of inflammatory cells was identified by the immunostaining of CD68+ cells. ELISA was employed to determine the inflammatory factors in the kidney and serum von Willebrand Factor (VWF). Tubular cell proliferation and apoptosis were identified by immunostaining. Renal fibrosis was assessed by Masson's tri-chrome straining and alpha-smooth muscle actin (α-SMA) staining. The CX3CL1 expression in the kidney was measured by immunostaining and Western blot, respectively. In vitro, human umbilical vein endothelial cells were treated with or without MVs for 24 or 48 hours under hypoxia injury to test the CX3CL1 by immunostaining and Western blot. Results: After administration of hWJMSC-MVs in acute kidney injury (AKI) rats, renal cell apoptosis was mitigated and proliferation was enhanced, inflammation was also alleviated in the first 48 hours. MVs also could suppress the expression of CX3CL1 and decrease the number of CD68+ macrophages in the kidney. In the late period, improvement of renal function and abrogation of renal fibrosis were observed. In vitro, MVs could down-regulate the expression of CX3CL1 in human umbilical vein endothelial cells under hypoxia injury at 24 or 48 hours. Conclusions: A single administration of MVs immediately after ischemic AKI could ameliorate renal injury in both the acute and chronic stage, and the anti-inflammatory property of MVs through suppression of CX3CL1 may be a potential mechanism. This establishes a substantial foundation for future research and treatment. © 2014 Zou et al.; licensee BioMed Central Ltd. Source

Lou S.,Ningbo Institute of Materials Technology and Engineering | Ye J.-Y.,Ningbo Dingxin Biological Science | Li K.-Q.,Ningbo No.2 Hospital | Wu A.,Ningbo Institute of Materials Technology and Engineering

Four different sized gold nanoparticles (14 nm, 16 nm, 35 nm and 38 nm) were prepared to conjugate an antibody for a gold nanoparticle-based immunochromatographic assay which has many applications in both basic research and clinical diagnosis. This study focuses on the conjugation efficiency of the antibody with different sized gold nanoparticles. The effect of factors such as pH value and concentration of antibody has been quantificationally discussed using spectra methods after adding 1 wt% NaCl which induced gold nanoparticle aggregation. It was found that different sized gold nanoparticles had different conjugation efficiencies under different pH values and concentrations of antibody. Among the four sized gold nanoparticles, the 16 nm gold nanoparticles have the minimum requirement for antibody concentrations to avoid aggregation comparing to other sized gold nanoparticles but are less sensitive for detecting the real sample compared to the 38 nm gold nanoparticles. Consequently, different sized gold nanoparticles should be labeled with antibody under optimal pH value and optimal concentrations of antibody. It will be helpful for the application of antibody-labeled gold nanoparticles in the fields of clinic diagnosis, environmental analysis and so on in future. This journal is © The Royal Society of Chemistry 1212. Source

Wang J.,Ningbo No.2 Hospital
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation

The effect of using redundancy technique to improve reliability of the quality management of medical devices is discussed. Source

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