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Dong H.,Ningbo Medical Center Lihuili Hospital | Wang G.,Ningbo Medical Center Lihuili Hospital | Li Y.,Ningbo Medical Center Lihuili Hospital
National Medical Journal of China | Year: 2017

Objective: To explore the diagnostic value of multi b-value DWI (MB-DWI) in the identification of benign and malignant breast lesions. Methods: From October 2013 to December 2015, a total of 54 cases from Ningbo Medical Center Lihuili Hospital (22 benign cases with a total of 24 lesions, 32 malignant cases with a total of 38 lesions) were examined with conventional MR scan, single b-value DWI, and multiple b-value DWI (0, 10,20, 30,50, 100,200, 400,800, 1 200, 2 000, 3 000 s/mm2). GE ADW4.5 workstation was used to calculate the value of standard apparent diffusion coefficient (ADCstandard), slow apparent diffusion coefficient (ADCslow), fast apparent diffusion coefficient (ADCfast) and fraction of ADCfast (ffast). Receiver operating characteristic (ROC) curve was performed to evaluate those MB-DWI parameters above. Results: The ADCstandard, ADCslow and ffast value of malignant group were (0.761 ± 0.166) × 10-3 mmVs, (0.593 ± 0.144) × 10-3 mm2/s, 0.330 + 0.099 respectively, which were significantly lower than those of benign group (all P < 0.01), while the ADCfast value of malignant group was 4.379 × 10-3 mm2/s, significantly higher than that of benign group (P < 0.01). The area under curve (AUC) of ffast and ADCstandard were 0.799, 0.786 respectively and there is no statistically significant difference. The AUC of ADCslow, ADCfast were 0.719, 0.727, which were statistically significant different from the value of ffast or ADCstandard (all P < 0.01). Conclusions: All the parameters in MB-DWI can be used to distinguish benign from malignant breast lesions. The ffast and ADCstandard value have higher diagnostic value in the identification of benign and malignant breast lesions.


Qiu S.,Ningbo Medical Center Lihuili Hospital | Xia Z.,Ningbo Medical Center Lihuili Hospital | Li Q.,Ningbo Medical Center Lihuili Hospital | Ye D.,Ningbo Medical Center Lihuili Hospital | And 2 more authors.
Oncology Letters | Year: 2017

The aim of the present study was to investigate the expression levels of Yes-associated protein (YAP) in different grades of laryngeal squamous cell carcinoma (LSCC) tissues and vocal cord polyps tissues, and to investigate any correlations with clinical factors. The expression of YAP in 128 cases of LSCC and 10 cases of vocal cord polyps tissues was tested using immunohistochemistry. YAP was primarily present in the nucleus of LSCC and controls, whereas phosphorylated YAP expression was present in the cytoplasm. The results indi­cated that YAP expression was upregulated in LSCC samples compared with vocal cord polyps tissues. YAP expression was positively correlated with the malignant degree of LSCC (P<0.01) and a high level of YAP expression in LSCC tissues was correlated with pathological type, lymphatic metastasis and clinical stage. The present study provided evidence for the expression and localization of YAP in LSCC and vocal cord polyps tissues. Thus, YAP may be involved in the occurrence and development of LSCC as an oncogene. © 2017, Spandidos Publications. All rights reserved.


PubMed | Yinzhou Second Hospital, Ningbo University and Ningbo Medical Center Lihuili Hospital
Type: | Journal: BioMed research international | Year: 2015

This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential expression and help elucidate their mechanisms of action. Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs were shown to be significantly upregulated in RHD patients compared with controls, including miR-1183 and miR-1299. A total of 137 miRNAs, including miR-4423-3p and miR-218-1-3p, were significantly downregulated in RHD patients. Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma. Bioinformatic predictions were also made of differentially expressed miRNAs as biomarkers in RHD by databases and GO/pathway analysis. Furthermore, we investigated miR-1183 and miR-1299 expression in RHD patients with secondary pulmonary hypertension (PAH). Our findings identified an important role for miR-1299 as a direct regulator of RHD, while the observed difference in expression of miR-1183 between RHD-PAH patients with high or low pulmonary artery pressure suggests that miR-1183 overexpression may reflect pulmonary artery remodeling. miR-1183 and miR-1299 appear to play distinct roles in RHD pathogenesis accompanied by secondary PAH and could be used as potential biological markers for disease development.


PubMed | Ningbo University and Ningbo Medical Center Lihuili Hospital
Type: Journal Article | Journal: Clinical biochemistry | Year: 2016

CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), as a tumor suppressor gene, plays an important role in the suppression of cell growth and apoptosis. The goal of our study is to investigate the association between CMTM3 promoter methylation and laryngeal squamous cell carcinoma (LSCC).Using the bisulfite pyrosequencing technology, DNA methylation levels of seven CpG sites in CMTM3 promoter are measured in tumor tissues and their adjacent tissues of 76 male LSCC patients.Our results reveal a significantly elevated promoter methylation of CMTM3 in tumor tissues compared with their adjacent tissues (P<0.001). A breakdown analysis by age shows that significant association of CMTM3 promoter methylation with cancer risk is specific to the LSCC patients older than 55years (P<0.001) but not in the younger patients (P=0.305). Moreover, the association is only observed in the LSCC patients with smoking behavior (P=0.001). Breakdown analysis also shows that CMTM3 promoter methylation is associated with cancer risk among patients with stage I LSCC (P<0.001).In conclusion, our study indicates that elevated CMTM3 methylation is a risk factor in male LSCC patients, especially in the patients with age over 55years and with smoking behavior.


Liu K.,Ningbo Medical Center Lihuili Hospital | Tian H.,Ningbo Medical Center Lihuili Hospital | Yu K.-Z.,Ningbo Medical Center Lihuili Hospital | Shen W.-Y.,Ningbo Medical Center Lihuili Hospital | And 4 more authors.
Tumor Biology | Year: 2014

NAD(P)H: quinone oxidoreductase 1 (NQO1) is an important enzyme which can catalyze the two-electron reduction of quinoid compounds into hydroquinones. NQO1 Pro187Ser polymorphism can change the enzymatic activity of NQO1, and it has been proposed to be associated with risk of esophageal cancer. We performed a meta-analysis to examine the association between NQO1 Pro187Ser polymorphism and esophageal cancer. Odds ratio (OR) with a 95 % confidence interval (95 % CI) was used to assess the association. Twelve case-control studies with 1,725 cases with esophageal cancer and 2,341 controls were finally included in the meta-analysis. Overall, there was an obvious association between NQO1 Pro187Ser polymorphism and esophageal cancer (allele model: OR = 1.24, 95 % CI 1.06-1.46, P OR = 0.009; homozygote model: OR = 1.59, 1195 % CI 1.10-2.30, P OR = 0.013; dominant model: OR = 1.31, 95 % CI 1.05-1.64, P OR = 0.018). In the subgroup analysis by ethnicity, there was an obvious association between NQO1 Pro187Ser polymorphism and esophageal cancer in Asians but not in Caucasians. Therefore, the meta-analysis suggests that NQO1 Pro187Ser polymorphism is associated with esophageal cancer risk. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Wu Q.P.,Ningbo Medical Center Lihuili Hospital
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2012

To explore the diagnostic value of the measurement of serum Golgi protein 73 (GP73) in the diagnosis of hepatocellular carcinoma (HCC). Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum GP73 in the 81 cases of HCC, 71 cases of chronic hepatitis or cirrhosis (CH/LC) and 65 cases of healthy blood donors, and to evaluate the sensitivity and specificity in the diagnosis of HCC through the ROC curves. The average levels of serum GP73 in HCC, CH/LC and Normal groups were (152.67 +/- 33.59) ng/ml, (93.15 +/- 20.02) ng/ml and (58.95 +/- 17.29) ng/ml(o) After calculating through the ROC curves, 120 ng/ml was set as the optimal cut-off point, GP73 has a sensitivity of 77.80% and a specificity of 78.00%. GP73 as a serum marker in the diagnosis of HCC had a higher sensitivity than AFP, and the combined detection of GP73 and AFP could improve HCC diagnosis.


PubMed | Ningbo Medical Center Lihuili Hospital
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2014

quinone oxidoreductase 1 (NQO1) is an important enzyme which can catalyze the two-electron reduction of quinoid compounds into hydroquinones. NQO1 Pro187Ser polymorphism can change the enzymatic activity of NQO1, and it has been proposed to be associated with risk of esophageal cancer. We performed a meta-analysis to examine the association between NQO1 Pro187Ser polymorphism and esophageal cancer. Odds ratio (OR) with a 95% confidence interval (95% CI) was used to assess the association. Twelve case-control studies with 1,725 cases with esophageal cancer and 2,341 controls were finally included in the meta-analysis. Overall, there was an obvious association between NQO1 Pro187Ser polymorphism and esophageal cancer (allele model: OR=1.24, 95% CI 1.06-1.46, P OR=0.009; homozygote model: OR=1.59, 1195% CI 1.10-2.30, P OR=0.013; dominant model: OR=1.31, 95% CI 1.05-1.64, P OR=0.018). In the subgroup analysis by ethnicity, there was an obvious association between NQO1 Pro187Ser polymorphism and esophageal cancer in Asians but not in Caucasians. Therefore, the meta-analysis suggests that NQO1 Pro187Ser polymorphism is associated with esophageal cancer risk.


PubMed | Ningbo Medical Center Lihuili Hospital
Type: Journal Article | Journal: The Thoracic and cardiovascular surgeon reports | Year: 2014

Bronchoplastic procedures offer better long-term survival and quality of life with less morbidity and mortality than pneumonectomies. However, minimally invasive sleeve lobectomy is rarely reported, and the procedure requires more skill even for experienced hands. We report a total video-assisted thoracoscopic right upper sleeve lobectomy for a patient with centrally located lung cancer.


PubMed | Ningbo Medical Center Lihuili Hospital
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2014

To compare the application value between reduced field-of-view diffusion weighted imaging (rFOV DWI) and single-shot echo-planar-imaging diffusion weighted imaging (SS-EPI DWI) of the breast.78 cases (68 normal breast, 10 breast cancer) were canned with SS-EPI DWI (FOV 360 mm 360 mm), rFOV DWI1 (FOV 360 mm 180 mm) and rFOV DWI2 (FOV 280 mm 140 mm). Image quality and ADC values of breast were compared with three groups. SNR were compared with rFOV DWI1 and rFOV DWI2.The image quality score of 78 cases was 4.74 by rFOV DWI1, 3.59 by SS-EPI DWI and 3.55 by rFOV DWI2. The mean ADC value of 68 normal breast was 1.832 10 mm/s by SS-EPI DWI, 1.701 10 mm/s by rFOV DWI1 and 1.615 10 mm/s by rFOV DWI2. The mean ADC value of 10 breast cancer was 1.345 10 mm/s by SS-EPI DWI, 1.220 10 mm/s by rFOV DWI1 and 1.149 10 mm/s by rFOV DWI2.SNR of rFOV DWI1 images was much higher than FOV DWI2.Sizeable rFOV DWI has higher resolution, less distortion than SS-EPI DWI, rFOV DWI will be useful in the diagnosis of the breast diseases.


PubMed | Ningbo Medical Center Lihuili Hospital
Type: Journal Article | Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology | Year: 2012

To explore the diagnostic value of the measurement of serum Golgi protein 73 (GP73) in the diagnosis of hepatocellular carcinoma (HCC).Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum GP73 in the 81 cases of HCC, 71 cases of chronic hepatitis or cirrhosis (CH/LC) and 65 cases of healthy blood donors, and to evaluate the sensitivity and specificity in the diagnosis of HCC through the ROC curves.The average levels of serum GP73 in HCC, CH/LC and Normal groups were (152.67 +/- 33.59) ng/ml, (93.15 +/- 20.02) ng/ml and (58.95 +/- 17.29) ng/ml(o) After calculating through the ROC curves, 120 ng/ml was set as the optimal cut-off point, GP73 has a sensitivity of 77.80% and a specificity of 78.00%.GP73 as a serum marker in the diagnosis of HCC had a higher sensitivity than AFP, and the combined detection of GP73 and AFP could improve HCC diagnosis.

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