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Ningbo, China

Wang A.-S.,Ningbo University | Xu C.-W.,Ningbo University | Xu C.-W.,HIGH-TECH | Xie H.-Y.,Ningbo University | And 9 more authors.
Journal of Functional Foods | Year: 2016

Docosahexaenoic acid (DHA) possesses various bioactivities. However, the effects of DHA on adipocyte homeostasis remain unclear. Here the effects of DHA (0, 50, 100, 150 μM) on the apoptosis of mature 3T3-L1 adipocytes was investigated in order to elucidate potential mechanisms. It was shown that DHA decreased adipocyte viability, induced apoptosis, and reduced the cellular lipid content. DHA treatment also caused mitochondrial membrane potential (MMP) loss and caspase-activation, as well as the up-regulation of pro-apoptotic protein Bad and an increase in the Bax/Bcl-ratio. Additionally, treatment with DHA down-regulated the levels of CREB, total Akt, p-Akt, total ERK, and p-ERK. Our data confirmed that DHA induced mitochondria-dependent apoptosis in 3T3-L1 adipocytes and that the CREB/Akt and ERK signalling pathways were involved in DHA-triggered apoptosis, which provides new information for the development of anti-obesity strategies. © 2016 Elsevier Ltd. Source

Yao A.,Ningbo University | Shen Y.,Ningbo University | Wang A.,Ningbo University | Chen S.,Ningbo University | And 7 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Sulforaphane (SFN), an isothiocyanate isolated from cruciferous vegetables, possesses anti-oxidant and anti-cancer bioactivities. Moreover, SFN exerts its pro-apoptotic effects in some cancer lines. However, the effects and mechanisms of SFN on the regulation of apoptosis of adipocytes are still unknown. In this study, we found that SFN induced significant apoptosis in 3T3-L1 adipocytes and markedly decreased the cellular lipid content. Western blot demonstrated that SFN-induced apoptosis was mediated via the mitochondrial apoptosis pathway based on increased cleavage of poly-ADP-ribose-polymerase (PARP), release of cytochrome c into the cytoplasm, and activation of caspase-3, as well as decreased Bcl-2/Bax ratio. In addition, SFN markedly decreased phosphorylation of Akt and downstream proteins, p70s6k1 and Bad, and increased phosphorylation of ERK. Therefore, our findings clarified that SFN could induce 3T3-L1 adipocyte apoptosis via down-regulation of the Akt/p70s6k1/Bad pathway and up-regulation of the ERK pathway, suggesting SFN may be a promising agent for the treatment or prevention of obesity. © 2015 Elsevier Inc. Source

Cheng J.,Zhejiang University | Wang Y.,148Th Hospital of PLA | Zhou K.,Zhejiang University | Wang L.,Bank of Blood Products | And 11 more authors.
PLoS ONE | Year: 2014

Objective: The goal of our study was to investigate whether DRD4 gene DNA methylation played an important role in the susceptibility of Han Chinese SCZ. Methods: Using the bisulphite pyrosequencing technology, DNA methylation levels of 6 CpG dinucleotides in DRD4 CpG island were measured among 30 paranoid SCZ patients, 30 undifferentiated SCZ patients, and 30 age- and gender-matched healthy controls. Results: Strong correlation was observed among the six CpG sites (r>0.5, P<0.01), thus average methylation levels were applied thereafter. Our results indicated that there was a significant association between DRD4 methylation and the risk of SCZ (P = 0.003), although there was no significant difference in DRD4 methylation between the two SCZ subtypes (P = 0.670). A breakdown analysis by gender showed that the significant association of DRD4 methylation and SCZ was driven by males (P<0.001) but not by females (P = 0.835). DRD4 methylation was significantly associated with p300 in male SCZ patients (r = 20.543, P = 0.005) but not in female SCZ patients (r = 0.110, P = 0.599). Moreover, receiver operating characteristic (ROC) curves showed DRD4 methylation was able to predict the status of SCZ in males [area under curve (AUC) = 0.832, P = 0.002] but not in females (AUC = 0.483, P = 0.876). Finally, a further expression experiment showed that DRD4 methylation in the gene body was positively associated with gene expression, although the exact mechanism of gene regulation remained unknown for this interesting DRD4 methylation. Conclusion: The gender disparity in the DRD4 DNA methylation provides novel insights into the pathogenesis of SCZ. © 2014 Cheng et al. Source

Chen X.-S.,Shanghai JiaoTong University | Tang Y.-X.,Shanghai University | Xiao Z.-P.,Shanghai JiaoTong University | Wang J.-J.,Shanghai JiaoTong University | And 6 more authors.
Chinese Medical Journal | Year: 2010

Background: Prior research about N400 has been mainly based on English stimuli, while the cognitive processing of Chinese characters is still unclear. The aim of the present study was to further investigate the semantic processing of Chinese idioms. Methods: Event related potentials (ERP) component N400 was elicited by 38 pairs of matching (congruent) and mismatching (incongruent) ended Chinese idioms: ending words with same phoneme but different shape and meaning (sPdSdM), with similar shape but different phoneme and meaning (sSdPdM), with same meaning but different phoneme and shape (sMdPdS), and words with different phoneme, shape and meaning (dPdSdM) and recorded by Guangzhou Runjie WJ-1 ERP instruments. In 62 right-handed healthy adults (age 19-50 years), N400 amplitudes and latencies were compared between matching and mismatching conditions at Fz, Cz and Pz. Results: N400 showed a midline distribution and could be elicited in electrodes Fz, Cz and Pz. The mean values of N400 latencies and amplitudes were obtained for matching and mismatching ending words in healthy adults. Significant differences were found in N400 latencies and amplitudes in matching and mismatching ending-words idioms in healthy adults (P <0.05). Compared with matching ending-words idioms, N400 latencies were prolonged and the amplitudes were increased in mismatching ones. N400s elicited by different types of stimuli showed different latencies and amplitudes, and longest N400 latency and largest N400 amplitude were elicited by ending-words with dPdSdM. No gender difference was found of N400 latency and amplitude in this study (P >0.05). Conclusions: Compared with English stimuli, Chinese ideographic words could provide more flexible stimuli for N400 research in that the words have 3-dimension changes - phoneme, shape and meaning. Features of N400 elicited by matching and mismatching ending words in Chinese idioms are mainly determined by the meaning of the word. Some issues of N400 elicited by Chinese characters deserve further research. Source

Zhang J.,Wenzhou University | Su H.,Wenzhou University | Tao J.,Wenzhou University | Xie Y.,Wenzhou University | And 6 more authors.
Addictive Behaviors | Year: 2015

Objective: High level of impulsivity as well as depression is thought to be involved in the maintenance and development of methamphetamine (METH) addiction. However, the relationship between impulsivity and depression has not been studied thoroughly in METH dependence subjects, especially in early METH abstinent subjects. In this study, our objective is to explore the interplay between the depressive symptoms and impulsivity in early METH abstinent subjects. Methods: A total of 182 early abstinent METH dependent subjects (abstinence for 1-7. days) were recruited and the level of impulsivity was measured by the Barratt Impulsiveness Scale (BIS-11). Depressive symptoms and anxiety symptoms were assessed by the short 13-item Beck Depression Inventory (BDI-13) and Beck Anxiety Inventory (BAI) respectively. Results: Global impulsivity of BIS-11 was significantly correlated with depressive symptoms among early METH abstinent subjects (r. = 0.283, p. = 0.001). Moreover, all subscales of BIS-11 were also found to be correlated with depressive symptoms: correlation with attentional impulsivity (r. = 0.202, p. = 0.006); correlation with motor impulsivity (r. = 0.267, p. = 0.001); and correlation with non-planning impulsivity (r. = 0.177, p. = 0.017). Conclusions: This study showed a relationship between impulsivity and depression, which may further the comprehension of motivational elements contributing to the maintenance and development of METH use disorder. Future research would be dedicated to exploring underlying mechanisms of association between impulsivity and depression. © 2014 Elsevier Ltd. Source

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