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Zheng B.,Zhejiang University | Jiang X.,Zhejiang University | Cheng H.,Zhejiang University | Xu Z.,Ningbo institute of Microcirculation and Henbane | And 3 more authors.
Journal of Biotechnology | Year: 2015

Lactobacillus heilongjiangensis DSM 28069T is a potential probiotic isolated from traditional Chinese pickle. Here we report the complete genome sequence of this strain. The complete genome is 2,790,548bp with the GC content of 37.5% and devoid of plasmids. Sets of genes involved in the biosynthesis of riboflavin and folate were identified in the genome, which revealed its potential application in biotechnological industry. The genome sequence of L. heilongjiangensis DSM 28069T now provides the fundamental information for future studies. © 2015.

Wang Y.,Zhejiang University | Jiang X.,Zhejiang University | Xu Z.,Ningbo institute of Microcirculation and Henbane | Ying C.,Zhejiang University | And 2 more authors.
Canadian Journal of Infectious Diseases and Medical Microbiology | Year: 2016

A 63-year-old-man was admitted to our hospital with severe subungual abscess. Bacteria were isolated from pus samples, and an inconsistent identification was shown by VITEK 2 system and MALDI-TOF mass spectrometry as Raoultella planticola and Raoultella terrigena, respectively. Molecular identification by 16S rRNA sequencing suggested that the isolate is R. Terrigena, and this was further demonstrated by sequencing three housekeeping genes (rpoB, gyrA, and parC) with phylogenetic analysis. To our knowledge, this is the first report of subungual abscess caused by R. Terrigena, a rare case of human infection due to soil bacterium. Our study highlights the technique importance on this pathogen identification. © 2016 Yu Wang et al.

Zheng B.,Zhejiang University | Jiang X.,Zhejiang University | Xu Z.,Ningbo institute of Microcirculation and Henbane | Fang Y.,Zhejiang University | Li L.,Zhejiang University
Extremophiles | Year: 2016

In this study, a novel metallo-β-lactamases fold hydrolase PH-1 was identified from Pelagibacterium halotolerans B2T. This novel member of the family Hyphomicrobiaceae was isolated from the East China Sea. In silico analysis demonstrated that PH-1 and its relative homologues cluster in a unique branch and constitute a new subgroup among MBLs. PH-1 was cloned and overexpressed in Escherichia coli BL21 in a soluble form. SDS-PAGE, MALDI-TOF/TOF–MS, and size-exclusion chromatography analysis demonstrated that the PH-1 was a monomer with molecular weight of about 29 kDa. Substrate specificity study showed PH-1 preferred penicillin type β-lactams and exhibited maximum activity toward penicillin-G. Additionally, our experiments also revealed that PH-1 was a halotolerant enzyme since it is active under 4 M NaCl. The enzyme activity of PH-1 was negatively affected by 1 mM Mn2+ and EDTA. These observations lay a foundation for further study of MBLs from marine bacterium. © 2015, Springer Japan.

Xu X.,Zhejiang University | Wang L.,Bank of Blood Products | Liao Q.,Zhejiang University | Chang L.,Zhejiang University | And 9 more authors.
PLoS ONE | Year: 2013

Aims:The aim of this study was to evaluate the combined contribution of 8 polymorphisms to the risk of Alzheimer's disease (AD).Methods:Through a comprehensive literature search for genetic variants involved in the AD association study, we harvested a total of 6 genes (8 polymorphisms) for the current meta-analyses. These genes consisted of A2M (5bp I/D and V1000I), ABCA2 (rs908832), CHAT (1882G >A, 2384G >A), COMT (Val158Met), HTR6 (267C >T) and LPL (Ser447Ter).Results:A total of 33 studies among 9,453 cases and 10,833 controls were retrieved for the meta-analyses of 8 genetic variants. It was showed that A2M V1000I (odd ratio (OR) = 1.26, 95% confidence interval (CI) = 1.07-1.49, P = 0.007), rs908832 allele of ABCA2 (OR = 1.55, 95% CI = 1.12-2.16, P = 0.009), 2384G >A of CHAT (OR = 1.22, 95% CI = 1.00-1.49, P = 0.05) and Ser447Ter of LPL in the Northern-American population (OR = 0.56, 95% CI = 0.35-0.91, P = 0.02) were significantly associated with the risk of AD. No association was found between the rest of the 5 polymorphisms and the risk of AD.Conclusion:Our results showed that A2M V1000I polymorphism in German, Korean, Chinese, Spanish, Italian and Polish populations, rs90883 of ABCA2 gene in French, American, Swiss, Greek and Japanese populations, 2384G >A of CHAT gene in British and Korean populations and LPL Ser447Ter in the Northern-American population were associated with the risk of AD. © 2013 Xu et al.

Zheng B.,Zhejiang University | Zheng B.,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases | Jiang S.,Zhejiang University | Xu Z.,Ningbo institute of Microcirculation and Henbane | And 4 more authors.
Brazilian Journal of Infectious Diseases | Year: 2015

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) are increasingly causing infective endocarditis over the past decade. Here we report a healthy man who developed a severe acute infective endocarditis with systemic embolism caused by CA-MRSA. The strain was recovered from repeated blood cultures and was characterized using molecular detection and genotyping. The S. aureus isolate was typed as ST630 SCC. mecV with spa-type t4549, agrI/IV and was PVL-negative. This is the only case report, to our knowledge, of CA-MRSA infective endocarditis in China. This case highlights the emergence and geographical spread of life-threatening CA-MRSA infection within China. © 2014.

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