Wang H.-K.,Fudan University |
Song X.-S.,Liaoning Medical University |
Cheng Y.,First Hospital of Ningbo |
Qu Y.-Y.,Fudan University |
And 12 more authors.
BJU International | Year: 2014
Objective To investigate whether visceral obesity is associated with certain histological subtypes of renal cell carcinoma (RCC) in a multicentre Chinese cohort. Patients and Methods A kidney tumour database was created using three tertiary centres in China; 487 patients were enrolled presenting with localised RCC and complete computer tomography (CT)/magnetic resonance imaging (MRI) information. A single-slice CT image was used to measure the area of visceral and subcutaneous adipose tissues in each patient. Statistical methods were used to analyse clear-cell RCC (ccRCC) and non-clear-cell RCC (non-ccRCC) as they relate to visceral fat area (VFA) and other risk factors, such as age, gender, tumour size, diabetes, hypertension, total fat area (TFA) and body mass index (BMI). Results In all, 418 patients had a ccRCC subtype and 69 had a non-ccRCC subtype. For all the patients with RCC, the mean VFA was 102 cm2, while mean BMI was 24 kg/m2. The mean VFA was greater in ccRCC than non-ccRCC patients by 25 cm2. There were significant differences in the mean VFA and TFA between patients with ccRCC and those with non-ccRCC. Multivariate analysis showed that the presence of VFA was more important than the effects of BMI and Type 2 diabetes on pathology prediction. In patients with a normal BMI, those with a higher quartile of VFA were more likely to develop ccRCC than those with a low VFA. Conclusions Increased visceral fat was found to be associated with ccRCC and the significance of VFA outweighed the effects of BMI and Type 2 diabetes for the prediction of RCC pathology in multivariate analyses. As a result, VFA could constitute a primary explanation for the link between obesity and ccRCC. © 2013 The Authors. BJU International © 2013 BJU International.
Tang S.,First Hospital of Ningbo
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012
To evaluate diagnostic significance of the noninvasive and objective 320-slice CT scan in the high pharynx resilience in the patients with obstructive sleep apnea/hypopnea syndrome. Fifty patients with OSAHS and 10 patients with pharyngeal paraesthesia were included in the study. 320 slice CT scan was used to measure the sagittal diameters, transverse diameters and sectional area of the retropalatal region, retroglossal region. Calculating the pharynx wall resilience. Analyzing the correlativity of pharynx wall resilience, BMI and AHI. Fiber endoscope was used to assess the subsidence percentage of the pharynx wall. Between OSAHS patients and pharyngeal paraesthesia patients, there was obviously difference in the retropalatal region and retroglossal region upper airway. The pharynx wall resiliences of retropalatal region were much more than that of retroglossal region. The later pharynx wall resiliences were much more than the fore-to-aft pharynx wall resiliences in the retropalatal region. BMI, AHI and the pharynx wall resilience were positive correlative to each other. But AHI and the pharynx wall resilience was nonlinear correlative. There was a good concordance between the pharynx wall resilience detected by 320CT and subsidence percentage of the pharynx wall detected by fiber endoscope. 320 slice CT is a kind of simple, rapid and objective method to localize the high resilience area of upper respiratory tract, which can be benefit to the clinical diagnosis, the etiological study and the treatment of OSAHS. Based on physical examination, we propose that only one time of CT scan to the patients' upper airway at the Muller' stage is enough to localize the stenosis area, which can reduce radiation dosage dramatically.
Wang Y.,Ningbo University |
Huang C.-S.,First Hospital of Ningbo |
Chen Y.-J.,First Hospital of Ningbo
National Medical Journal of China | Year: 2013
Objective: Aim to determine the effect of different dose of 6% 130/0.4 hydroxyethyl starch on coagulation function in rats model with severe sepsis by cecal ligation and puncture (CLP). Method: 80 male SD rats were selected and randomly divided into the sham groups and the CLP groups, every group was divided into 4 small Groups again; before recovery (H0), 7.5 ml/kg group (H1), 15 ml/kg group (H2) and 30 ml/kg group (H3), n=10; The CLP groups were established by cecal ligation and perforation (CLP), the sham groups were the same as the CLP groups except for CLP, MAP was monitored for all rats during the experiment. After 4 hours by CLP, Procedure fluid resuscitation which received 6% hydroxyethyl starch 130/0.4 by respectively 7.5 ml/kg, 15 ml/kg, 30 ml/kg were started at a rate of 10 ml · kg-1 · h-1, the sham groups were the same dose 6% hydroxyethyl starch 130/0.4 at the same time. All rats were taken for arterial blood harvest at 4 h after fluid infusion for arterial blood gases, blood routine examination, conventional coagulation function and thrombelastography (TEG). Results: Compared with the sham groups, PO2 in the CLP groups was not significantly different. pH, PCO2 and MAP were significantly lower than the sham groups, Lac was significantly higher than the sham groups (P < 0.05). APTT and PT were significantly higher than the sham groups (P < 0.05), D-Dime and FIB were not significant different (P > 0.05), R and K were significantly shorter than the sham groups, MA was significantly higher than the sham groups (P < 0.05), α and CI were higher than the sham groups, but the difference was not statistically significant (P > 0.05). Within CLP group, compared with H0, R, K, α, MA and CI after fluid resuscitation were not significant different. Conclusion: Low-dose (less than 30 ml/kg) 6% hydroxyethyl starch 130/0.4 in early fluid resuscitation have less effect on coagulation function in rats with severe sepsis. Copyright © 2013 by the Chinese Medical Association.
Wang J.-J.,First Hospital of Ningbo |
Zhang L.-M.,First Hospital of Ningbo |
Dai Y.-P.,First Hospital of Ningbo |
Guo Y.,First Hospital of Ningbo
Chinese Journal of Cancer Prevention and Treatment | Year: 2010
OBJECTIVE, To research the effects of SMYD3 silencing on invasiveness in human breast cancer MCF-7 cells and investigate its possible mechanism. METHODS: Eukaryotic expression plasmids of microRNA targeting SMYD3 were constructed and stably transfected into MCF-7 cells. The expressions of SMYD3 mRNA and protein levels, and WNT10B mRNA were respectively detected by polymerase chain action (RT-PCR) and Western blot. The plate clone formation experiment and Matrigel invasion assay were used to detect the cell proliferation and invasiveness in vitro. RESULTS: The DNA sequencing results indicated that the microRNA sequences inserted into pcDNA6. 2-GW/EmGFPmiR eukaryotic expression vector completely met the design. RT-PCR and Western blot detection results showed that compared with the control group, SMYD3 expressions were significantly inhibited in the two SMYD3-targeted groups (P<0. 05) , and had no significant difference in the negative control group (P>0. 05). The Matrigel in vitro invasion assay revealed that the mean invasion percentages of the cells of the two SMYD3-targeted groups (7. 53±2. 00, 9. 13±2. 50) were significantly lower than those of the control group cells (63. 40 ±6. 85) and the negative control group cells (66. 69±4. 63, P<0. 05). The plate clone formation rates of the two SMYD3-targeted groups (16. 5%, 18. 7%) were significantly decreased in comparison with the control group (79. 4%, P<0. 05), and there was no significantly difference between those of the negative control group (76. 6%) and the control group (P>0. 05). CONCLUSIONS: The over-expression of SMYD3 can markedly upgrade the invasion of the breast cancer MCF-7 cells, and cause the transfer of the cells. That may possibly be related to that SMYD3 activates the abnormal high expression of its downstream oncogene WNT10B and finally causes epithelial-mesenchymal transition.