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Jin M.-J.,Ningbo Fiber Inspection Institute | Ruan Y.,Ningbo Fiber Inspection Institute | Yan B.-B.,Shanghai Majorbio Bio pharm Technology Co. | Shi D.-H.,Ningbo Fiber Inspection Institute
Wool Textile Journal | Year: 2011

Extraction method of mtDNA is the key technical problem about research and development the DNA testing for animal fiber. This paper studys the extraction method of mtDNA for cashmere and wool, and validates through PCR enlarge. The results show that use the Promega kit, it is favorable to next step that makeing the sample powder as much as possible, and first remove the undissolved fibers through centrifugal after cracking, then the sampte gained can be met the ruquirments for PCR enlarge and DNA sequencing. Source


Jin M.-J.,Ningbo Fiber Inspection Institute | Ruan Y.,Ningbo Fiber Inspection Institute | Cai H.-K.,Shanghai Majorbio Bio pharm Technology Co. | Qian W.-J.,Ningbo Fiber Inspection Institute
Wool Textile Journal | Year: 2012

There are some differences in the DNA sequence among different species. Living environment, food chain and other factors can also caused genetic mutations. This paper analysed the mtDNA sequence, based on testing for different regions or kinds of cashmere and wool fibers, then gave the results as follows;the similarity of mtDNA sequence between cashmere and wool was more than 98% , but in specific locations there had their own characteristic base sequence; the characteristic base sequence of mtDNA of cashmere fiber, from different regions or kinds, was almost the same, and so did the wool. Source


Qian W.-J.,Ningbo Fiber Inspection Institute | Zhang J.-B.,Ningbo Fiber Inspection Institute | Zhang W.-Y.,Ningbo Fiber Inspection Institute | Ye L.,Ningbo Fiber Inspection Institute
Wool Textile Journal | Year: 2012

A method has been developed for the determination of the permethrin content in wool fabrics with GC-MS. The permethrin was determined with column HP-5 MS ( 30 m × 0. 25 mm × 0. 25 μm). The results showed that cis-trans permethrin was well separated, the recoveries were between 88.0% ∼94.3% , and the RSDs were between 5.4% ∼9.7%. In addition, as the column HP-5 MS (30 m ×0. 25 mm ×0. 25 μm) was also used for detection of banned azo dyes in textiles, testers save the disassembly time and greatly improve the detection efficiency. This method was suitable for high volume testing. Source

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