Ningbo Academy of Agricultural science

Ningbo, China

Ningbo Academy of Agricultural science

Ningbo, China
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Li R.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Wang Y.,Beijing Advanced Innovation Center for Food Nutrition and Human Health | Ling J.,Ningbo Academy of Agricultural science | Liao X.,Beijing Advanced Innovation Center for Food Nutrition and Human Health
Food Chemistry | Year: 2017

The effects of high pressure processing (HPP) on the activity of soluble acid invertase (SAI) in mango pulp, crude extract, purified SAI and purified SAI in model systems (pectin, bovine serum albumin (BSA), sugars and pH 3–7) were investigated. The activity of SAI in mango pulp was increased after HPP, and that in crude extract stayed unchanged. The activity of purified SAI was decreased after HPP at 45 and 50 °C. Pectin exhibited a concentration-dependent protection for purified SAI against HPP at 50 °C/600 MPa for 30 min. Pectin that had an esterification degree (DE) of 85% exhibited a greater protection than pectin that had a DE of 20–34%. BSA, acidic pH (3–6) and sucrose also exhibited protection for purified SAI against HPP. HPP at 50 °C/600 MPa for 30 min disrupted the secondary structure and tertiary structure of purified SAI, but no aggregation of purified SAI was observed after HPP. © 2017 Elsevier Ltd


Li C.,Hebei University of Engineering | Wu Y.-L.,Ningbo Academy of Agricultural science | Yang T.,Ningbo Academy of Agricultural science | Huang-Fu W.-G.,Ningbo Academy of Agricultural science
Journal of Chromatographic Science | Year: 2012

A simple, sensitive, and reliable analytical method is developed for the rapid determination of fumonisin B1 and fumonisin B2 in corn by high-performance liquid chromatographypositive electrospray ionization tandem mass spectrometry (LC-ESI-M-SMS). Fumonisin B1 and fumonisin B2 are extracted from corn with methanolwater (3:1, v/v) by means of ultrasonic extraction, and directly injected into an LC-MS-MS system after centrifugation. Fumonisin B1 and fumonisin B2 are separated on a Zorbax Eclipse XDB-C18 column with a solution of methanolwaterformic acid as the mobile phase. The method is validated with respect to linearity, accuracy, precision, specificity, and stability. Moreover, the method was applied to real samples and demonstrated to be suitable for the determination of fumonisin B1 and fumonisin B2 in corn. The total time required for the analysis of one sample was ∼30 min. © 2011 The Author.


Hou X.-L.,China Agricultural University | Wu Y.-L.,Ningbo Academy of Agricultural science | Yang T.,Ningbo Academy of Agricultural science | Du X.-D.,Henan Agricultural University
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

A simple and cost-effective pre-treatment procedure was developed for 18 sulfonamides in pork using dispersive solid phase extraction (dSPE) with multi-walled carbon nanotubes (MWCNTs). The sample was analysed after purification by ultra high-performance liquid chromatography-positive electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS). After extraction with phosphate buffer (pH 6.0), a dSPE procedure was conducted with MWCNTs. The pH value of the extract, extraction time with MWCNTs, type and amount of MWCNTs and type of eluent were optimised to increase the sample throughput and sensitivity. The samples were quantified using sulfamethazine-13C6 as an internal standard. The recoveries of the target compounds from the pork samples were most efficient when 150mg of MWCNTs with an outer diameter of less than 8nm and a length of 0.5-2μm was used. A mixture of acetonitrile/50mM ammonium acetate (95:5, v/v) was shown to be the most suitable solvent for desorbing the compounds from the MWCNTs. The proposed method was validated according to the European Commission Decision 2002/657/EC, which determines linearity, specificity, decision limit (CCα), detection capability (CCβ), recovery, precision and stability. © 2013 Elsevier B.V.


Yang Q.-F.,National Animal Husbandry Services | Wu Y.-L.,Ningbo Academy of Agricultural science
Chromatographia | Year: 2012

A fast analytical method for the simultaneous determination of fumonisin B1 (FB 1) and fumonisin B 2 (FB 2) in corn using a novel QuEChERS method and LC-MS-MS was developed and validated. Samples were extracted with methanol-water (3:1 v/v) by means of ultrasonic extraction. The extract was purified with a novel modified QuEChERS method. Firstly, FB 1 and FB2 in the extract were retained with primary secondary amine (PSA). Then, FB1 and FB 2 were released from PSA with 1.0 % formic acid in methanol. The final eluate was diluted with water, and analyzed by LC-MS-MS on a Waters Acquity BEH C 18 column with 0.1 % formic acid in water/methanol as mobile phase with gradient elution. Mean recoveries of 83.5-102.4 % with CVs of 3.6-10.5 % were obtained at fortification levels of 2, 50 and 1,000 μg kg -1. The limit of quantification was 2.0 μg kg -1. © 2012 Springer-Verlag.


Zhang M.-X.,Hebei University of Engineering | Li C.,Hebei University of Engineering | Li C.,Tianjin Agricultural University | Wu Y.-L.,Ningbo Academy of Agricultural science
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2012

A simple, sensitive and reliable analytical method was developed for the determination of a new beta-agonist phenylethanolamine A in animal hair, tissues and animal feeds by ultra high performance liquid chromatography-positive electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) with QuEChERS. Samples were extracted with acetonitrile/water (80:20, v/v). The extract was purified through QuEChERS method, then was dried with nitrogen and residues were redissolved in mobile phase for hair sample or directly diluted with 0.1% formic acid in water for other samples, and analyzed by LC-MS/MS on a Waters Acquity BEH C 18 column with 0.1% formic acid in water/methanol as mobile phase with gradient elution. The samples were quantified using phenylethanolamine A-D 3 as internal standards. The proposed method was validated according to the European Commission Decision 2002/657/EC determining specificity, decision limit (CCα), detection capability (CCβ), recovery, precision, linearity, robustness and stability. The CCα values ranged from 0.10 to 0.26μg/kg. The CCβ values ranged from 0.20 to 0.37μg/kg. The mean recoveries of 95.4-108.9% with intra-day CVs of 2.2-5.6% and inter-day CVs of 3.1-6.2% were obtained. The method is demonstrated to be suitable for the determination of phenylethanolamine A in animal hair, tissues and animal feeds. The total time required for the analysis of one sample except animal hair sample, including sample preparation, was about 25min. © 2012 Elsevier B.V..


Du X.-D.,Henan Agricultural University | Wu Y.-L.,Ningbo Academy of Agricultural science | Yang H.-J.,Quality Control and Inspection Center for Domestical Animal Products | Yang T.,Ningbo Academy of Agricultural science
Journal of Chromatography A | Year: 2012

A simple and inexpensive pretreatment procedure was developed for 10 β2-agonists (clenbuterol, ractopamine, salbutamol, bambuterol, penbuterol, tulobuterol, clorprenaline, mabuterol, cimaterol and terbutaline) in swine urine using dispersive solid phase extraction (dSPE) with multi-walled carbon nanotubes (MWCNTs). The sample was analysed after purification by ultra high performance liquid chromatography-positive electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS). The pH value of the swine urine, extraction time, type and amount of MWCNTs and type of eluent were optimised to increase the sample throughput and sensitivity. The samples were quantified using clenbuterol-D9, ractopamine-D6 and salbutamol-D3 as internal standards. The recoveries of the target compounds from swine urine samples at pH 10.0 were most efficient when using 20mg of MWCNTs with a 30-50nm outer diameter and a length of 10-30μm, while a mixture of water/methanol (90:10, v/v) with 0.5% formic acid was shown to be the most suitable solvent for desorbing the compounds from the MWCNTs. The proposed method was validated according to the European Commission Decision 2002/657/EC, which determines linearity, specificity, decision limit (CCα), detection capability (CCβ), recovery, precision and stability. © 2012 Elsevier B.V.


Ying Y.-F.,Zhejiang University | Wu Y.-L.,Ningbo Academy of Agricultural science | Wen Y.,Chinese Research Academy of Environmental Sciences | Yang T.,Ningbo Academy of Agricultural science | And 2 more authors.
Journal of Chromatography A | Year: 2013

A simple and cost-effective pre-treatment procedure was developed for six resorcylic acid lactones (RALs) in feed using dispersive solid phase extraction (dSPE) with multi-walled carbon nanotubes (MWCNTs). The sample was analysed after purification by ultra-high performance liquid chromatography-negative electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS). After extraction with acetonitrile/water (80:20, v/v) and dilution with water, a dSPE procedure was carried out with MWCNTs. The pH value of the extract, the extraction time for MWCNTs, the type and amount of MWCNTs and the type of eluent were optimised to increase the sample throughput and the sensitivity. The samples were quantified using the internal standard zearalenone-D6. The absolute recoveries of the target compounds from feed samples were most efficient when using 100mg of MWCNTs with an outer diameter of less than 8nm and a length of 10-30μm, and ethyl acetate was shown to be the most suitable solvent for desorbing the target compounds from the MWCNTs. The mean recoveries from fortified swine mixed feed samples ranged from 95.3% to 107.2% and had relative standard deviations lower than 10%; the limits of detection and quantification for RALs were in the ranges of 0.20-0.29μg/kg and 0.54-0.78μg/kg, respectively. © 2013 Elsevier B.V.


Chen X.-B.,Shanghai JiaoTong University | Wu Y.-L.,Ningbo Academy of Agricultural science | Yang T.,Ningbo Academy of Agricultural science
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011

A simple and sensitive analytical method was developed for the simultaneous determination of clenbuterol, chloramphenicol and diethylstilbestrol in bovine milk by isotope dilution ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Samples were directly purified through HLB cartridge. The organic phase was dried under nitrogen and residues were redissolved in mobile phase. Samples were analyzed by UPLC-MS/MS on an Acquity UPLC® BEH C18 column with gradient elution. The samples were quantified using clenbuterol-D9, chloramphenicol-D5 and diethylstilbestrol-D8 as internal standards. The proposed method was validated according to the European Union regulation 2002/657/EC determining specificity, decision limit (CCα), detection capability (CCβ), trueness, precision, linearity and stability. The method is demonstrated to be suitable for the determination of clenbuterol, chloramphenicol and diethylstilbestrol in bovine milk. The total time required for the analysis of one sample was about 50min. © 2011 Elsevier B.V.


Zhang J.-M.,Zhejiang Academy of Agricultural Sciences | Wu Y.-L.,Ningbo Academy of Agricultural science | Lu Y.-B.,Zhejiang Academy of Agricultural Sciences
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

A simple, sensitive and reliable analytical method was developed for the simultaneous determination of 22 carbamate insecticides and 17 mycotoxins in cereals by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS). Carbamates and mycotoxins were extracted from cereal samples using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure without any further clean-up step. The extract was diluted with water containing 0.1% formic acid and 5.0mM ammonium acetate, and analyzed by LC-MS/MS on a Waters Acquity BEH C18 column with water (0.1% formic acid, 0.50mM ammonium acetate)/methanol as mobile phase with gradient elution. Matrix-matched calibration was used for quantification. Blank samples (rice, wheat and corn) were fortified at 5, 10 and 50μg/kg except for five zearalenonic compounds at 25, 50 and 250μg/kg, and recoveries were in the range of 70-120%. Relative standard deviations were lower than 20% in all cases. The LOQ values were in the range of 0.20-29.7μg/kg. The method is suitable for the simultaneous determination of carbamate insecticides and mycotoxins in cereals. The total time required for the analysis of one sample, including sample preparation, was about 35min. © 2013.


Li C.,Hebei University of Engineering | Yang T.,Ningbo Academy of Agricultural science | Huangfu W.,Ningbo Academy of Agricultural science | Wu Y.,Ningbo Academy of Agricultural science
Chemosphere | Year: 2011

The fate of pymetrozine was studied in rice field ecosystem, and a simple and reliable analytical method for determination of pymetrozine in soil, rice straw, paddy water and brown rice was developed. Pymetrozine residues were extracted from samples, cleaned up by solid phase extraction (SPE) and then determined by high-performance liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS). The average recovery was 81.2-88.1% from soil, 83.4-88.6% from rice straw, 87.3-94.1% from paddy water and 82.9-85.3% from brown rice. The relative standard deviation (RSD) was less than 15%. The limits of detection (LODs) of pymetrozine calculated as a sample concentration were 0.0003mgkg -1 (mg L -1) for soil and paddy water, 0.001mgkg -1 for brown rice and rice straw. The results of kinetics study of pymetrozine residue showed that pymetrozine degradation in water, soil, and rice straw coincided with C=0.194e -0.986t, C=0.044e -0.099t, and C=0.988e -0.780t, respectively; the half-lives were about 0.70d, 7.0d and 0.89d, respectively. The degradation rate of pymetrozine in water was the fastest, followed by rice straw. The highest final pymetrozine residues in brown rice were 0.01mgkg -1, which was lower than the EU's upper limit of 0.02mgkg -1 in rice. Therefore, a dosage of 300-600ga.i.hm -2 was recommended, which could be considered as safe to human beings and animals. © 2010 Elsevier Ltd.

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