Saste S.R.,Nimbkar Agricultural Research Institute |
Ghalsasi P.M.,Nimbkar Agricultural Research Institute |
Kataria R.S.,National Bureau of Animal Genetic Resources |
Joshi B.K.,National Bureau of Animal Genetic Resources |
And 2 more authors.
Indian Journal of Biotechnology | Year: 2012
Authors have standardized and report here a simplified amplification refractory mutation system (ARMS)-PCR test as an alternative technique to PCR-RFLP for detection of FecB genotype in sheep. An ARMS-PCR test to detect the FecB mutation in Black Bengal goats has recently been described in the literature. A number of modifications to this technique has been made to simplify and standardize the protocol to use it for sheep DNA, isolated from blood samples on FTA paper. The modifications included reducing the PCR reaction volume to half of original protocol, using a single touchdown annealing temperature and combining two PCR amplicons amplified with different primer pairs (one for each allele of FecB) for each sample prior to loading on the gel. These modifications can make the technique cheaper and more suitable for large scale, rapid FecB genotyping in sheep for use in FecB introgression breeding programmes.
PubMed | National Bureau of Animal Genetic Resources and Nimbkar Agricultural Research Institute
Type: | Journal: Animal biotechnology | Year: 2017
The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development. Variable expression was observed for almost all the genes included in the present study among high and low fecundity groups that was most significant for the BMP7, BMP4, LHCGR, and FSHR transcripts in the ovarian follicles of high and low fecundity ewes, indicating their importance in governing the fecundity in FecB carrier, Indian Garole sheep. BMP4 expression among the genes studied was significantly higher in FecB carrier Garole sheep. This study confirms the changes in mRNA expression of the genes implicated in follicular development in FecB carrier and noncarrier Indian sheep breeds.
Rajvanshi A.K.,Nimbkar Agricultural Research Institute |
Patil S.M.,Nimbkar Agricultural Research Institute |
Ranaware A.M.,Nimbkar Agricultural Research Institute |
Chopade V.J.,Nimbkar Agricultural Research Institute |
Rajvanshi N.,Columbia University
12th International Conference on Indoor Air Quality and Climate 2011 | Year: 2011
Lanstove, a unique cooking and lighting device for rural areas has been presented in this paper. The specifications and various parameters of Lanstove are presented. Detailed comments and feedback from testing in actual user dwellings have shown that Lanstove can provide tremendous improvement in quality of life of rural poor. An attempt has been made to compare the Lanstove with conventional electric cooking and lighting methods. An energy analysis showed that electric cooking and lighting will consume 3 times more energy than Lanstove. This analysis has been backed by the results of Life Cycle Assessment (LCA), which showed that the environmental impacts such as global warming potential and acidification potential from production and operation of electric bulb and stove are much higher than those of Lanstove.
Anaytullah,Banaras Hindu University |
Anaytullah,Nimbkar Agricultural Research Institute |
Srivastava A.K.,Banaras Hindu University |
Bose B.,Banaras Hindu University
Vegetos | Year: 2012
In present investigation, a field experiment was undertaken to find out the effects of nitrate (Mg (NO3.)2 and KNO3) seed hardening (a kind of seed priming) treatment on some biochemical and physiological parameters i.e. chlorophyll, nitrogen, protein and proline contents, nitrate reductase (NR) and super oxide dismutase (SOD) activities and on yield attributes like harvest index(H.I.), biological yield, grain yield and test weight of wheat (Trticum aestivum L.) varieties (HUW-234 and HUW-468) sown in different dates i.e. 30th Nov., 15th Dec. and 30th Dec., representing timely sown (D1), late sown (D2) and very late sown (D3) of its cropping seasons respectively. Plants raised from nitrate hardened seeds showed increments in the chlorophyll, nitrogen, protein and proline contents, NR and SOD activities in each date of observation (OD) in both the varieties of wheat. This trend was noted to be maintained in each date of sowing. Peak values for nitrogen, protein, enzyme NR and chlorophyll content observed on 50 and 75 DAS respectively. In between the salts of nitrate Mg (NO3)2 hardened set performed better in comparison to KNO3 treated set in improving H.I., grain yield, biological yield and the test weight but the plants raised from non-hardened (control) seeds always showed poor performance. Nitrate treated sets exhibited an improvement for all the above mentioned parameters even in late and very late sown conditions of wheat in comparison to non-hardened control set. Thus on the basis of results it can be recommended that Mg (NO3)2 is the most suitable salt to be used in enhancing the performance of wheat varieties i.e.HUW-234 and HUW -468 sown in time as well as in late or very late of its cropping season.
Ranaware A.,Nimbkar Agricultural Research Institute |
Singh V.,Nimbkar Agricultural Research Institute |
Nimbkar N.,Nimbkar Agricultural Research Institute
Indian Journal of Natural Products and Resources | Year: 2010
The antifungal activity of aqueous extracts of seven plant species, viz. bulbs of Garlic (Allium sativum Linn.) and leaves of Datura (Datura metel Linn.), Lantana (Lantana camara Linn.), Eucalyptus (Eucalyptus hybrid), Neem (Azadirachta indica A. Juss.), Madagascar periwinkle (Catharanthus roseus G. Don) and Holy basil (Ocimum sanctum Linn.) against safflower leaf spot disease-causing fungus, Alternaria carthami, under in vitro conditions was investigated. Linear growth reduction of the pathogen on PDA plates at 20% plant extract concentration was recorded seven days after inoculation. A. sativum bulb extract exhibited maximum inhibition of the pathogenic fungus (48.68%) followed by D. metel (44.25%), while O. sanctum extract showed the least inhibitory effect (25.02%). The observations indicate the efficacy of aqueous plant extracts as potential inhibitors of Alternaria carthami.