Entity

Time filter

Source Type

Kyoto, Japan

Takahashi A.,Hokkaido University | Watanabe J.,Japan National Agriculture and Food Research Organization | Sakaguchi H.,Nihonshinyaku Co. | Okazaki Y.,Fuji Womens University | And 2 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2015

Aronia (Aronia melanocarpa E.) and haskap (Lonicera caerulea L.) fruits contain a number of bioactive phenolic phytochemicals, such as cyanidin 3-galactoside (aronia) and cyanidin 3-glucoside (haskap). We investigated the effects of anthocyanin-enriched aronia and haskap extracts (aronia extract and haskap extract) on potassium bromate (KBrO3)-induced renal and hepatic damage in rats. Treatment with aronia and haskap extracts (anthocyanin intake, 300 mg/kg B.W.) prior to KBrO3 administration (125 mg/kg B.W.) significantly inhibited kidney weight increases compared to the control (saline alone) rats. Blood urea nitrogen (BUN) and serum creatinine as well as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities of rats administered the aronia extract were significantly lower than those of control rats, indicating that the aronia extract suppressed KBrO3-induced renal and hepatic damage. In addition, aronia and haskap extracts recovered decreases in oxygen radical absorbance capacity (ORAC) levels of serum and kidneys, and KBrO3-induced increases in thiobarbituric acid reactive substance (TBARS) level of kidneys. These results suggest that aronia and haskap extracts ameliorated KBrO3-induced kidney and liver damage via enhancing antioxidant capacities in vivo. Copyright © 2015, Japanese Society for Food Science and Technology. Source


Takahashi A.,Hokkaido University | Okazaki Y.,Fuji Womens University | Nakamoto A.,Fuji Womens University | Watanabe S.,Fuji Womens University | And 7 more authors.
Journal of Oleo Science | Year: 2014

Haskap (Lonicera caerulea L.) fruit contains some bioactive phenolic phytochemicals, mainly cyanidin-3-glucoside (cy3-glc) and chlorogenic acid. The purpose of this study was to investigate the effects of anthocyanin-rich phenolic phytochemical (containing 13.2% anthocyanin) purified from a Haskap fruit (named Haskap phytochemical) on postprandial serum triglyceride and blood glucose levels. The Haskap phytochemical (containing cy 3-glc at 300 mg/kg of body weight) was administered orally to rats fasted for 24 h and 30 min later, a corn oil emulsion was administered to these rats. After the administration, serum triglyceride concentration was measured. An increase in serum triglyceride concentration and the AUC significantly lowered in the Haskap phytochemical-administered group than in the saline-administered group. To evaluate the effect of serum glucose levels, the Haskap phytochemical was orally administered to rats fasted for 24 h and sucrose solution (2 g/kg of body weight) was administered to these rats after 30 min. After the administration, blood glucose level was measured. The Haskap phytochemical significantly reduced the increase in blood glucose levels and AUC in the Haskap phytochemical-administered group than in the saline-administered group. Furthermore, to investigate the long-term effects of Haskap phytochemical intake, high-fat diet (HF diet) with 1.5% or 3.0% Haskap phytochemical was administered to rats for four weeks. The investigation of chronological changes in the serum components of the rats fed HF diets in addition to the administration of Haskap phytochemical showed that the increase in serum triglyceride concentrations, total cholesterol concentrations and blood glucose were significantly suppressed compared to the HF diet-fed control (HF-control). These results suggest that the decrease in postprandial blood lipids and blood glucose by short or long-term Haskap phytochemical ingestion is due to anthocyanin and other polyphenols contained in the Haskap phytochemical. © 2014 by Japan Oil Chemists' Society. Source


Takahashi A.,Hokkaido University | Shimizu H.,Fuji University | Okazaki Y.,Fuji University | Sakaguchi H.,Nihonshinyaku Co. | And 3 more authors.
Journal of Oleo Science | Year: 2015

Aronia fruits (chokeberry: Aronia melanocarpa E.) containing phenolic phytochemicals, such as cyanidin 3-glycosides and chlorogenic acid, have attracted considerable attention because of their potential human health benefits in humans including antioxidant activities and ability to improved vision. In the present study, the effects of anthocyanin-rich phytochemicals from aronia fruits (aronia phytochemicals) on visceral fat accumulation and fasting hyperglycemia were examined in rats fed a high-fat diet (Experiment 1). Total visceral fat mass was significantly lower in rats fed aronia phytochemicals than that in both the control group and bilberry phytochemicals-supplemented rats (p < 0.05). Moreover, perirenal and epididymal adipose tissue mass in rats fed aronia phytochemicals was significantly lower than that in both the control and bilberry phytochemicals group. Additionally, the mesenteric adipose tissue mass in aronia phytochemicals-fed rats was significantly low (p < 0.05). Furthermore, the fasting blood glucose levels significantly decreased in rats fed aronia phytochemicals for 4 weeks compared to that in the control rats (p < 0.05). Therefore, we investigated the effects of phytochemicals on postprandial hyperlipidemia after corn oil loading in rats, pancreatic lipase activity in vitro, and the plasma glycemic response after sucrose loading in order to elucidate the preventive factor of aronia phytochemical on visceral fat accumulation. In the oral corn oil tolerance tests (Experiment 2), aronia phytochemicals significantly inhibited the increases in plasma triglyceride levels, with a half-maximal inhibitory concentration (IC50) of 1.50 mg/mL. However, the inhibitory activity was similar to that of bilberry and tea catechins. In the sucrose tolerance tests (Experiment 3), aronia phytochemicals also significantly inhibited the increases in blood glucose levels that were observed in the control animals (p < 0.05). These results suggest that anthocyanin-rich phytochemicals in aronia fruits suppress visceral fat accumulation and hyperglycemia by inhibiting pancreatic lipase activity and/or intestinal lipid absorption. © 2015 by Japan Oil Chemists’ Society. Source

Discover hidden collaborations