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Hashima, Japan

Hitomi A.,Asahikasei Medical Co. | Futono K.,Asahikasei Medical Co. | Serizawa R.,Asahikasei Medical Co. | Kato Y.,Asahikasei Medical Co. | And 3 more authors.
Japanese Pharmacology and Therapeutics | Year: 2012

Objective: Promotive effects of autologous fibrin glue (CryoSeal) produced by CryoSeal FS System (ThermoGenesis, USA) were compared with those of commercially available fibrin glues. Methods: Fibrin glues were embedded at the base of incised wound of abdominal skin of rats. Adhesive strength of the glues was measured using a tensile strength tester at 30 min, 24hrs and 7 days after embedding. Histopathological examination was undertaken on the skin samples collected at 7 days post embedding. Incised abdominal skins without fibrin glue embedding were served as control. Results: Tensile strength gradually increased on time in all groups and they were consistently higher in the skins treated with fibrin glues in comparison with control. Among the fibrin glues, autologous fibrin glue showed a significantly higher adhesive effect comparing with other commercially available fibrin glues. Histopathologically, fibrin glues promoted wound healing by producing more amount of and matured granulation tissue under the regenerated epidermis covering the surface of wound. The autologous fibrin glue was absorbed and induced the proliferation of granulation tissue more promptly than commercially available fibrin glues. Conclusion: Autologous fibrin glue promoted a rapid wound healing and contributed to higher tensile strength of incised wound of the skin in comparison with commercially available fibrin glues. The autologous fibrin glue was considered safe regarding the risks of contaminant infection/anaphylaxis and more effective tissue adhesive than commercially available fibrin glues. Source


Kasai S.,Eisai Co. | Ito A.,Ito Bone Histomorphometry Institute | Shindo K.,Canon Inc. | Toyoshi T.,Nihon Bioresearch Inc | Bando M.,Eisai Co.
PLoS ONE | Year: 2015

Oxidative stress affects bone turnover. Preventative effects of antioxidants such as vitamin E on reduced bone mineral density and fractures associated with aging, osteoporosis, and smoking have been examined in animals and humans. The effects of vitamin E (α-tocopherol; αT) on bone health have yielded conflicting and inconclusive results from animal studies. In this study, to determine the bone effects of αT, we investigated the in vivo effects of αT on the bone mineral density, bone mass, bone microstructure, bone resorption, and osteogenesis through peripheral quantitative computed tomography (pQCT) measurements, micro-computed tomography (micro-CT) analyses, and bone histomorphometry of lumbar vertebrae and femurs in normal female Wistar rats fed diets containing αT in different quantities (0, 30, 120, or 600 mg/kg diet) for 8 weeks. To validate our hypotheses regarding bone changes, we examined ovariectomized rats as an osteoporosis model and control sham-operated rats in parallel. As expected, ovariectomized rats had reduced bone mineral density in lumbar vertebrae and the distal metaphyses of their femurs, reduced bone mass and deteriorated microstructure of cancellous bones in the vertebral body and distal femur metaphyses, and reduced bone mass due to resorption-dominant enhanced bone turnover in secondary cancellous bones in these sites. In comparison, αT administered to normal rats, even at the highest dose, did not induce reduced bone mineral density of lumbar vertebrae and femurs or a reduced bone mass or fragile microstructure of cancellous bones of the vertebral body and distal femur metaphyses. Instead, αT-fed rats showed a tendency for an osteogenesis-dominant bone mass increase in secondary cancellous bones in the vertebral body, in which active bone remodeling occurs. Thus, αT consumption may have beneficial effects on bone health. © 2015 Kasai et al. Source


Yoshimura M.,Kikkoman Corporation | Toyoshi T.,Nihon Bioresearch Inc | Sano A.,Kikkoman Corporation | Izumi T.,Kikkoman Corporation | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

This study aimed to investigate the effects of a γ-aminobutyric acid (GABA) rich tomato (Solanum lycopersicum L.) cultivar 'DG03-9' in comparison with 'Momotaro', a commonly consumed tomato cultivar in Japan, on systolic blood pressure (SBP) in spontaneously hypertensive rats (SHR). In a single administration study, treatment with the GABA-rich cultivar elicited a significant decrease in SBP compared to the control group. In a chronic administration study, SHR were fed diets containing one of the tomato cultivars for 4 weeks. Both cultivars significantly reduced the increase In SBP compared to the control. The antihypertensive effect of the GABA-rich cultivar was higher than that of the commonly consumed cultivar in both the single- and chronic-administration studies. Treatment with a comparable amount of GABA elicited a similar response to treatment with the GABA-rich cultivar. These results suggest that the GABA-rich cultivar 'DG03-9' is a potent antihypertensive food and may be useful for treating hypertension effectively. © 2009 American Chemical Society. Source


Takakura S.,Astellas Pharma Inc. | Toyoshi T.,Nihon Bioresearch Inc | Hayashizaki Y.,Astellas Pharma Inc. | Takasu T.,Astellas Pharma Inc.
Life Sciences | Year: 2016

Aims We investigated the effect of the selective sodium-dependent glucose cotransporter 2 inhibitor ipragliflozin on the simultaneous progression of diabetic microvascular complications of retinopathy, nephropathy and neuropathy in individual Spontaneously Diabetic Torii (SDT) fatty rats. Main methods Ipragliflozin was administered to male SDT fatty rats for 12 weeks. Male Sprague-Dawley rats of the same age were used as non-diabetic controls. Non-fasting plasma glucose and glycated hemoglobin levels were measured every 4 weeks. Cataract formation was monitored once a week, and the electroretinogram was measured after 6 weeks of treatment. After the treatment period, motor nerve conduction velocity was measured and urinalysis was conducted. Tissue samples were then dissected for histopathological examination. Key findings Treatment with ipragliflozin reduced glycated hemoglobin levels, inhibited the progression of cataract formation, prevented the prolongation of oscillatory potential peaks in the electroretinogram, ameliorated the slowing of motor nerve conduction velocity, and reduced the severity of glomerulosclerosis in SDT fatty rats. Significance These results suggest that the control of hyperglycemia with ipragliflozin slows the progression of the diabetic complications of retinopathy, nephropathy, and neuropathy. © 2016 Elsevier Inc. All rights reserved. Source


Ohata A.,Otsuka Pharmaceutical Factory Inc. | Tamura N.,Otsuka Pharmaceutical Factory Inc. | Iwata K.,Otsuka Pharmaceutical Factory Inc. | Abe N.,Otsuka Pharmaceutical Factory Inc. | And 4 more authors.
Journal of Surgical Research | Year: 2014

Background Preventing interbowel adhesions still remains a challenge. Peritoneal mesothelial damage can induce postoperative adhesions. Our study evaluated the effects of 3% trehalose solution on mesothelial protection and adhesion prevention. Also, we compared this novel solution with Seprafilm regarding efficacy. Methods Mesothelial damage was induced on the cultured human mesothelial cell (Met-5A) and rabbit cecum-serosal surface by air-drying for 60 min, and trehalose solution was applied. Cell integrity was tested by measuring lactate dehydrogenase, and serosal-morphologic changes were analyzed using scanning electron microscopy. Intra-abdominal adhesions were induced in rabbits by the combination of abrasion and air-drying procedures. Animals were divided into four groups: control, 3% trehalose solution, Seprafilm, and 3% trehalose solution with Seprafilm. Adhesions were evaluated blindly 7 d later. Results Lactate dehydrogenase release from the Met-5A cells was reduced dose-dependently by trehalose (P < 0.05). Morphologic studies clearly showed that mesothelial cells on the serosal surface were kept intact by 3% trehalose solution. In a rabbit adhesion model, 3% trehalose solution reduced adhesions between bowel and bowel or bowel and surrounding structures (P < 0.01 versus control and Seprafilm). Seprafilm reduced adhesions between abdominal wall and underlying viscera (P < 0.01 versus control and 3% trehalose solution). Three-percent trehalose solution with Seprafilm showed additive effects of adhesion prevention, reducing adhesion formation at the previously mentioned sites. Conclusions Three-percent trehalose solution protects mesothelial cells and leads to reduced adhesions between bowel and bowel or bowel and surrounding structures. This effect seems to be resulted from the characteristics of the solution covering most areas that potentially develop adhesions. © 2014 Elsevier Inc. All rights reserved. Source

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