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Afieroho O.E.,Nigerian National Institute for Pharmaceutical Research and Development | Okorie O.,University of Port Harcourt | Okonkwo T.J.N.,University of Port Harcourt
Tropical Journal of Pharmaceutical Research | Year: 2012

Purpose: To develop a simple and cost effective spectrophotometer method for the determination of ACE inhibitor ramipril in dosage forms. Methods: UV spectrophotometry was used to develop and validate a simple method for the assay of ramipril in solid dosage form at λ max of 210 nm, as per International Conference on Harmonization (ICH) guidelines. Aqueous methanol (5%) was used as the blank solvent. The method was validated for linearity, recovery, accuracy, precision, specificity in the presence of excipients, and also for inter-day stability under laboratory conditions. Results: Validation results showed linearity in the range 1 - 38 μg/ml; recovery accuracy 101.55%; regression equation Y = 0.0256X + 0.0697, R 2 of 0.9942; precision RSD < 2.00%; and negligible interference from common excipients and colorants. The method was accurate (95% confidence limit) compared to standard liquid chromatography (LC) method, with comparable reproducibility when used to assay a commercial product (Ramitace ®, 2 and 5 mg tablets). Conclusion: The validated data were within allowable limits and therefore, the proposed method is recommended for routine quality control (QC) analysis. © Pharmacotherapy Group. Source


Ibekwe T.S.,University Of Abuja | Ramma L.,University of Cape Town | Chindo B.A.,Nigerian National Institute for Pharmaceutical Research and Development
Journal of Laryngology and Otology | Year: 2012

Background: In the management of sensorineural hearing loss, effective therapy for degenerated hair cells, third order neurons, ganglions, dendrites and synaptic areas of the vestibulo-cochleo-cerebral pathway remains an enigma. Transplantation of stem and progenitor cells appears to be an emerging potential solution, and is the focus of this review.Aim: To review recent developments in the management of sensorineural hearing loss in the field of stem cell research.Materials and method: A systematic review of the English language literature included all experimental and non-experimental studies with a Jadad score of three or more, published between 2000 and 2010 and included in the following databases: Cochrane Library Ear, Nose and Throat Disorders; Medline; Google Scholar; Hinari; and the Online Library of Toronto University.Results: Of the 455 and 29 600 articles identified from Medline and Google Scholar, respectively, 48 met the inclusion criteria. These were independently reviewed and jointly analysed.Conclusion: Although there is not yet any evidence from successful human studies, stem cell and 'alternative stem cell' technology seems to represent the future of sensorineural hearing loss management. © Copyright JLO (1984) Limited 2012. Source


Rodrigues A.,CSIR - National Chemical Laboratory | Emeje M.,CSIR - National Chemical Laboratory | Emeje M.,Nigerian National Institute for Pharmaceutical Research and Development
Carbohydrate Polymers | Year: 2012

Starch has found use in industries as diverse as food, textiles, cosmetics, plastics, adhesives, paper, and pharmaceuticals. From a pharmaceutical standpoint, starch finds its value in solid-oral dosage forms, where it has been used as a binder, diluent, and disintegrant. However, only recently has the use of starch in nanotechnology started to make significant advances in biomedical applications, including newer drug delivery techniques. There has been a considerable effort to develop biodegradable nanoparticles as effective drug delivery systems. Being cheap, non-toxic, renewable, biodegradable and compatible with many other materials for industrial applications, starch is attracting the interest of drug delivery scientists. We have put together in a short and concise format, recent applications of starch derivatives in the emerging field of nanodrug delivery with the conclusion that a lot still needs to be done. © 2011 Elsevier Ltd. All rights reserved. Source


Chime S.A.,University of Nigeria | Attama A.A.,University of Nigeria | Builders P.F.,Nigerian National Institute for Pharmaceutical Research and Development | Onunkwo G.C.,University of Nigeria
Journal of Microencapsulation | Year: 2013

Objective: To formulate sustained-release diclofenac potassium-loaded solid lipid microparticles (SLMs) based on solidified reverse micellar solution (SRMS) and to evaluate the in vitro and in vivo properties. Methods: SRMS consisting of mixtures of Phospholipon® 90H and Softisan® 154 were used to formulate diclofenac potassium-loaded SLMs. Characterization based on the particle size and morphology, stability and encapsulation efficiency (EE%) were carried out on the SLMs. In vitro release was carried out in simulated intestinal fluid (pH 7.5). Anti-inflammatory and ulcerogenic properties were studied using rats. Results: Maximum EE% of 95%, 94% and 93% were obtained for SLMs formulated with SRMS 1:1, 2:1 and 1:2, respectively. In vitro release showed about 85-90% drug release at 13h. Diclofenac potassium-loaded SLMs showed good anti-inflammatory and gastro-protective properties. Conclusion: Diclofenac potassium-loaded SLMs based on SRMS could be used orally or parenterally under controlled conditions, for once daily administration. © 2013 Informa UK Ltd. Source


Kalita R.D.,Tezpur University | Nath Y.,Tezpur University | Ochubiojo M.E.,Tezpur University | Ochubiojo M.E.,Nigerian National Institute for Pharmaceutical Research and Development | Buragohain A.K.,Tezpur University
Colloids and Surfaces B: Biointerfaces | Year: 2013

Microcrystalline cellulose (MCC) is generally produced through acid hydrolysis of woody plants and agro sources. MCC synthesized from a common wild grass Setaria glauca (L) P. Beauv was characterized to explore the possibility of application in pharmaceutical industry especially as a drug delivery vehicle. The SEM, TGA, XRD and FTIR investigations of the prepared MCC reveal that the 5-30μm long, non aggregated MCC rods have high crystallinity index of 80% and were stable at 286 °C. The preliminary investigation of the MCC incorporated micro beads containing isoniazid, one of the first line drugs for treatment of tuberculosis was carried out in the simulated intestinal fluid (SIF). The MCC incorporated micro beads with isoniazid drug load showed sustained release upto 24. h with release of 0.521μg of isoniazid equivalent drug in the SIF system. No cytotoxicity of the MCC was observed in the haemolytic assay. The MCC also showed good antioxidant activity. Thus, the study reveals that the MCC can be prepared from an inexpensive and abundant grass species. The MCC have properties advantageous for application in the pharmaceutical industry and may be explored further in drug delivery research. © 2013 Elsevier B.V. Source

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