Nichihara Research and Development Laboratories Inc

Shimane, Japan

Nichihara Research and Development Laboratories Inc

Shimane, Japan
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Abe S.,Iwate University | Abe S.,Nichihara Research and Development Laboratories Inc | Yamamoto K.-I.,Iwate University | Yamamoto K.-I.,Chiyoda Corporation | And 5 more authors.
Journal of Insect Biotechnology and Sericology | Year: 2014

An entomogenous fungus, Cordyceps militaris, grows on larvae and pupae of the silkworm, Bombyx mori. To overcome problems in the establishment of fungal infection caused by advancing pupal-adult development, we tested the effects of carbon dioxide (CO2) anesthesia. Pupae inoculated with C. militaris were held in CO2anesthesia for 2 days. Almost all pupae exposed to CO2were infected with C. militaris, but the rate of infection of chilled or untreated pupae decreased with increasing stage of pupal-adult development at inoculation. The results indicate that exposure of silkworm pupae to CO2enhances infection rate. This treatment will support the mass production of C. militaris for pharmacological uses. © 2014, Japanese Society of Sericultural Sciences. All rights reserved.

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