News Article | February 22, 2017
No statistical methods were used to predetermine sample size. The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment. Male heterozygous ythdf2+/− fish in the *AB background were custom made by ZGeneBio. TALEN mutagenesis was performed to mutate ythdf2 (Ensembl ENSDART00000127043) with L1 recognition sequence 5′-GGACCTGGCCAATCCCC-3′, R1 recognition sequence 5′-GGCACAGTAATGCCACC-3′, and spacer sequence 5′-TCCCAATTCAGGAATG-3′. Purchased fish were outcrossed to in-house wild-type *AB fish. Embryos were obtained from natural crosses, were raised under standard conditions, and were staged according to literature26. Embryos were reared at 28.5 °C and all experiments and observations were performed as close to this temperature as possible. Fish lines were maintained in accordance with AAALAC research guidelines, under a protocol approved by the University of Chicago IACUC (Institutional Animal Care & Use Committee). The open reading frame of zebrafish ythdf2 was purchased from Open Biosystems (clone 5601005) and subcloned into a pCS2+ vector using restriction enzyme sites of BamHI and XhoI. The resulting vector was linearized by HindIII and used as a template for ythdf2 probe preparation. Antisense digoxigenin (DIG) RNA probes were generated by in vitro transcription using standard reagents and methods. In situ hybridization protocol was followed essentially as previously reported27. All experiments were repeated at least once from biological samples. Control and ythdf2 morpholinos (5′-TGGCTGACATTTCTCACTCCCCGGT-3′) were obtained from Gene Tools (Oregon). 3 ng of either control or gene-specific morpholino was injected into *AB wild-type embryos at the one-cell stage. GFP and mCherry were subcloned into pCS2+ vectors and linearized by NotI. GFP-m6A, GFP-A, and mCherry-capped and polyadenylated mRNA was generated by in vitro transcription using mMessage mMachine SP6 kit (Thermo Fisher) and Poly(A) tailing kit (Thermo Fisher) according to the manufacturer’s protocol. Products were purified with the MEGAclear transcription clean-up kit (Thermo Fisher) and used for injections directly. For GFP-m6A, we spiked 6 nmol m6ATP into the 100 nmol ATP supplied in the transcription reaction, in order to ensure that less than 0.3% of GFP mRNAs are without m6A on average. (GFP mRNA is 942 nt; each mRNA has 1.89 m6A on average.) 35 pg of either GFP reporter mRNA and 10 pg of mCherry mRNA were injected together in 1.25 nl into embryos at the one-cell stage. ythdf2 mRNA containing the ythdf2 5′ UTR and a 3′ Flag tag, which was used to rescue the mutant phenotype and validate the knockdown efficiency of ythdf2 MO, was constructed in pCS2+ vector (forward primer: 5′-CGTACGGATCCTGTCTGATCTGCAGCTGTAG-3′; reverse primer: 5′-CGATGCTCGAGTTACTTGTCATCGTCGTCCTTGTAATCTATTCCAGATGGAGCAAGGC-3′) and prepared in the same way as mCherry mRNAs. Antibodies used in this study are listed below in the format of name (application; catalogue number; supplier): mouse anti-Flag HRP conjugate (Western; A5892; Sigma), rabbit anti-m6A (m6A-seq and m6A-CLIP-seq; 202003; Synaptic Systems), rabbit anti-histone H3 (IF; ab5176; Abcam), and anti-rabbit Alexa Fluor 488 (IF; ab150077; Abcam). All images were observed with a Leica MZFLIII microscope and captured with a Nikon D5000 digital camera using Camera Control Pro (Nikon) software. For fluorescent microscopy, standard ET-GFP and TXR LP filters (Leica) were used. For bright field imaging of live embryos, only saturation was adjusted and was adjusted identically for all images. For fluorescent imaging of live embryos, no image processing was performed. For fluorescent imaging of fixed embryos, contrast and exposure were adjusted for all to obtain the lowest amount of background while preserving the morphology of all visible nuclei. All experiments were repeated at least once from biological samples. To compare the total amount of DNA in wild-type and mutant embryos at different time points during the MZT, 10 embryos per time point per condition were dechorionated and pipetted into standard DNA lysis buffer. The number of embryos in each tube was counted twice to ensure uniformity. Proteinase K was added to 100 μg ml−1 and the embryos were incubated for 4 h at ~55 °C with occasional mixing. Proteinase K was inactivated by a 10-min incubation at 95 °C and the DNA was then phenol-chloroform-extracted, ethanol-precipitated, and resuspended in 100 μl Tris (pH 8.5) and 1 mM EDTA using standard procedures. Double-stranded DNA content was measured with NanoDrop. Three biological replicates (comprised of the offspring of three different fish mating pairs of the appropriate genotype) were measured for each time point for both the control and experimental samples. Biological replicates were averaged together to determine the average DNA amount per time point per genotype and to compute standard errors of the mean. All DNA values were normalized to that of wild-type embryos at 2.5 h.p.f. Embryos were collected into standard 2× protein sample buffer with added β-mercaptoethanol and protease inhibitors and immediately put on ice for a few minutes. The embryo mixtures were carefully but thoroughly pipetted up and down to dissolve and homogenize the embryos, and then samples were heated at 95 °C for 5 min and frozen at −80 °C. Before use, samples were again heated for 5 min and then centrifuged at 12,000 r.p.m. to remove debris. Supernatants were loaded into a 10-well, 1.5 mm Novex 4–20% Tris-Glycine Mini Protein Gel (Thermo Fisher) with 6 embryos per well. The gel was transferred onto a nitrocellulose membrane using iBlot2 gel transfer system (Thermo Fisher) set to P3 for 7 min with iBlot2 mini gel transfer stacks (Thermo Fisher). Membranes were blocked in 5% BSA, 0.05% Tween-20 in PBS for 1 h, and then incubated overnight at 4 °C with anti-Flag–HRP conjugate (Sigma) diluted 1:10,000 in 3% BSA. Proteins were visualized using the SuperSignal West Pico Luminol/Enhancer solution (Thermo Fisher) in FluorChem M system (ProteinSimple). mRNA isolation for LC-MS/MS: total RNA was isolated from zebrafish embryos with TRIzol reagent (Invitrogen) and Direct-zol RNA MiniPrep kit (Zymo). mRNA was extracted by removal of contaminating rRNA using RiboMinus Eukaryote Kit v2 (Thermo Fisher) for two rounds. Total RNA isolation for RT–qPCR: we followed the instruction of Direct-zol RNA MiniPrep kit (Zymo) with DNase I digestion step. Total RNA was eluted with RNase-free water and used for RT–qPCR directly. 100–200 ng of mRNA was digested by nuclease P1 (2 U) in 25 μl of buffer containing 10 mM of NH OAc (pH 5.3) at 42 °C for 2 h, followed by the addition of NH HCO (1 M, 3 μl, freshly made) and alkaline phosphatase (0.5 U). After an additional incubation at 37 °C for 2 h, the sample was diluted to 50 μl and filtered (0.22 μm pore size, 4 mm diameter, Millipore), and 5 μl of the solution was injected into LC-MS/MS. Nucleosides were separated by reverse-phase ultra-performance liquid chromatography on a C18 column with on-line mass spectrometry detection using an Agilent 6410 QQQ triple-quadrupole LC mass spectrometer in positive electrospray ionization mode. The nucleosides were quantified by using the nucleoside to base ion mass transitions of 282 to 150 (m6A), and 268 to 136 (A). Quantification was performed in comparison with the standard curve obtained from pure nucleoside standards running on the same batch of samples. The ratio of m6A to A was calculated on the basis of the calibrated concentrations9. Total RNA was isolated from fish embryos collected at different time points with TRIzol reagent and Direct-zol RNA MiniPrep kit. For each time point, ~200 embryos were collected to ensure RNA yield and that samples were representative. mRNA was further purified using RiboMinus Eukaryote Kit v2. RNA fragmentation was performed by sonication at 10 ng μl−1 in 100 μl RNase-free water using Bioruptor Pico (Diagenode) with 30 s on/off for 30 cycles. m6A-immunoprecipitation (IP) and library preparation were performed according to the previous protocol17. Sequencing was carried out on Illumina HiSeq 2000 according to the manufacturer’s instructions. Additional high-throughput sequencing of zebrafish methylome was carried out using a modified m6A-seq method, which is similar to previously reported methods19, 20. Briefly, total RNA and mRNA were purified as previously described for m6A-seq. Purified mRNA (1 μg) was mixed with 2.5 μg of affinity purified anti-m6A polyclonal antibody (Synaptic Systems) in IPP buffer (150 mM NaCl, 0.1% NP-40, 10 mM Tris-HCl (pH 7.4)) and incubated for 2 h at 4 °C. The mixture was subjected to UV-crosslinking in a clear flat-bottom 96-well plate (Nalgene) on ice at 254 nm with 0.15 J for 3 times. The mixture was then digested with 1 U μl−1 RNase T1 at 22 °C for 6 min followed by quenching on ice. Next, the mixture was immunoprecipitated by incubation with protein-A beads (Invitrogen) at 4 °C for 1 h. After extensive washing, the mixture was digested again with 10 U μl−1 RNase T1 at 22 °C for 6 min followed by quenching on ice. After additional washing and on-bead end-repair, the bound RNA fragments were eluted from the beads by proteinase K digestion twice at 55 °C for 20 and 10 min, respectively. The eluate was further purified using RNA clean and concentrator kit (Zymo Research). RNA was used for library generation with NEBNext multiplex small RNA library prep kit (NEB). Sequencing was carried out on Illumina HiSeq 2000 according to the manufacturer’s instructions. Total RNA was isolated from wild-type and mutant fish embryos collected at different time points with TRIzol reagent and Direct-zol RNA MiniPrep kit. For each time points, ~20 embryos were collected to ensure RNA yield and that samples were representative. mRNA was further purified using RiboMinus Eukaryote Kit v2. RNA fragmentation was performed using Bioruptor Pico as described previously. Fragmented mRNA was used for library construction using TruSeq stranded mRNA library prep kit (Illumina) according to manufacturer’s protocol. Sequencing was carried out on Illumina HiSeq 2000 according to the manufacturer’s instructions. All samples were sequenced by Illumina Hiseq 2000 with single-end 50-bp read length. The deep-sequencing data were mapped to zebrafish genome version 10 (GRCz10). (1) For m6A-seq, reads were aligned to the reference genome (danRer10) using Tophat v2.0.14 (ref. 28) with parameter -g 1–library-type = fr-firststrand. RefSeq Gene structure annotations were downloaded from UCSC Table Browser. The longest isoform was used if the gene had multiple isoforms. Aligned reads were extended to 150 bp (average fragments size) and converted from genome-based coordinates to isoform-based coordinates, in order to eliminate the interference from introns in peak calling. The peak-calling method was modified from published work18. To call m6A peaks, the longest isoform of each gene was scanned using a 100 bp sliding window with 10 bp step. To reduce bias from potential inaccurate gene structure annotation and the arbitrary usage of the longest isoform, windows with read counts less than 1 out of 20 of the top window in both m6A-IP and input sample were excluded. For each gene, the read counts in each window were normalized by the median count of all windows of that gene. A Fisher exact test was used to identify the differential windows between IP and input samples. The window was called as positive if the FDR < 0.01 and log (enrichment score) ≥ 1. Overlapping positive windows were merged. The following four numbers were calculated to obtain the enrichment score of each peak (or window): (a) reads count of the IP samples in the current peak or window, (b) median read counts of the IP sample in all 100 bp windows on the current mRNA, (c) reads count of the input sample in the current peak/window, and (d) median read counts of the input sample in all 100 bp windows on the current mRNA. The enrichment score of each window was calculated as (a × d)/(b × c). (2) For m6A-CLIP-seq, after removing the adaptor sequence, the reads were mapped to the reference genome (danRer10) using Bowtie2. Peak calling method was similar to the previous study19. Briefly, mutations were considered as signal and all mapped reads were treated as background. A Gaussian Kernel density estimation was used to identify the binding regions. The motif analysis was performed using HOMER29 to search motifs in each set of m6A peaks. The longest isoform of all genes was used as background. (3) For mRNA-seq, reads were mapped with Tophat and Cufflink (v2.2.1) was used to calculate the FPKM of each gene to represent their mRNA expression level30. (4) For fish gene group categorization, we used the input mRNA-seq data from m6A-seq. FPKM of all genes were first normalized to the highest value of five time points, with only genes with FPKM >1 analysed. Then Cluster3.0 (ref. 31) was used to divide all genes into six clusters, with the parameters: adjust data – normalize genes; k-means cluster – organize genes, 6 clusters, 100 number; k-means – Euclidean distance. The result clustered file with clustered number was merged with original FPKM values, imported and processed in R, and plotted in Excel. (5) For GO analysis, the list of target genes was first uploaded into DAVID32, 33 and analysed with functional annotation clustering. The resulting file was downloaded and extracted with GO terms and corresponding P values. The new list (contains GO terms with P < 0.01) was imported into REVIGO34 and visualized with the interactive graph, which was used as the final output figures. Methylated genes (at each time point) were defined as overlapped gene targets between m6A-seq and m6A-CLIP-seq. Ythdf2-regulated genes were defined as overlapped gene targets between the lists of the top 20% upregulated genes in both ythdf2 knockout and MO-injected samples. The most stringent Ythdf2 target genes at 4 h.p.f. (135) were defined in the main text, as overlapped genes of methylated genes at 4 h.p.f. (3,237) and Ythdf2-regulated genes at 4 h.p.f. (876). All the raw data and processed files have been deposited in the Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo) and accessible under GSE79213. A summary of sequenced samples and processed FPKM data are included as Supplementary Data 2. One set of representative experiment results from at least two independent experiments were shown where applicable. Quantitative reverse-transcription PCR (RT–qPCR) was performed to assess the relative abundance of mRNA. All RNA templates used for RT–qPCR were pre-treated with on-column DNase I digestion in the purification step. RT–qPCR primers were designed to span exon-exon junctions to only detect mature mRNA. RT–qPCR was performed by using SuperScript III one-step RT–PCR system (Thermo Fisher) with 50–100 ng total RNA template. Actb1 was used as an internal control as it showed relative invariant expression during the studied time period according to pilot RT–qPCR data. P values were determined using two-sided Student’s t-test for two samples with equal variance. *P < 0.05; **P < 0.01; ***P < 0.001. The sequences of primers used in this study are listed below: actb1: forward 5′-CGAGCAGGAGATGGGAACC-3′, reverse 5′-CAACGGAAACGCTCATTGC-3′; buc: forward 5′-CAAGTTACTGGACCTCAGGATC-3′, reverse 5′-GGCAGTAGGTAAATTCGGTCTC-3′; zgc:162879: forward 5′-TCCTGAATGTCCGTGAATGG-3′, reverse 5′-CCCTCAGATCCACCTTGTTC-3′; mylipa: forward 5′-CCAAACCAGACAACCATCAAC-3′, reverse 5′-CACTCCACCCCATAATGCTC-3′; vps26a: forward 5′-AAATGACAGGAATAGGGCCG-3′, reverse 5′-CAGCCAGGAAAAGTCGGATAG-3′; tdrd1: forward 5′-TACTTCAACACCCGACACTG-3′, reverse 5′-TCACAAGCAGGAGAACCAAC-3′; setdb1a: forward 5′-CTTCTCAACCCAAAACACTGC-3′, reverse 5′-CTATCTGAAGAGACGGGTGAAAC-3′; mtus1a: forward 5′-TGGAGTATTACAAGGCTCAGTG-3′, reverse 5′-TTATGACCACAGCGACAGC-3′; GFP: forward 5′-TGACATTCTCACCACCGTGT-3′, reverse 5′-AGTCGTCCACACCCTTCATC-3′. High-throughput sequencing data that support the findings of this study have been deposited at GEO under the accession number GSE79213. All the other data generated or analysed during this study are included in the article and Supplementary Information.
News Article | February 28, 2017
MastersinAccounting.info, a leading career and education website focused on graduate programs in accounting and finance, has released its ranking of the Top Online Master’s in Accounting Programs. To be considered for the list, schools with an online master’s in accounting program were checked for not-for-profit status and accreditation from one of the six regional accreditation agencies in the US recognized by the US Department of Education. The online degrees from the schools on the list are also the same degrees granted to traditional, on-campus students. The rankings were based on factors measuring academic quality, student experience, and graduate success. The ranking uses a unique methodology that considers such factors as the average tuition cost per online credit hour; program accreditation by the AACSB, ACBSP, or IACBE; the average mid-career pay of alumni; and school rankings according to US News & World Report in the regional, national, and online categories. Rob Voce, founder of MastersinAccounting.info, said about the list: “Enrollment in online degree programs is increasing and schools are responding by offering more distance education programs at the graduate level - which can be particularly convenient for those who are already working full-time. Our ranking is designed to help these prospective students learn about and compare first-rate online master’s in accounting programs that offer long-term value.” Overall, 37 schools with online master’s in accounting programs satisfied the inclusion requirements and ranked on this list. Auburn University, in Auburn, Alabama, captured the top spot on the list, followed by the University of North Carolina at Chapel Hill in the second spot. As well as providing schools’ results on ranking factors, the Top Online Master’s in Accounting Programs list includes detailed information on schools’ admissions statistics and requirements as well as tuition comparisons. For the top-ranking schools the list also provides: The top schools on this year’s list are: 1. Auburn University Raymond J. Harbert College of Business (Auburn, AL) 2. University of North Carolina Kenan-Flagler Business School (Chapel Hill, NC) 3. University of Connecticut School of Business (Storrs, CT) 4. University of Massachusetts Amherst Isenberg School of Management (Amherst, MA) 5. Pennsylvania State University World Campus (State College, PA) 6. University of Southern California Marshall School of Business (Los Angeles, CA) 7. Emporia State University School of Business (Emporia, KS) 8. Rutgers, The State University of New Jersey Business School (New Brunswick, NJ) 9. Colorado State University College of Business (Fort Collins, CO) 10. University of Alabama at Birmingham Collat School of Business (Birmingham, AL) 11. University of Texas at Dallas Naveen Jindal School of Business (Richardson, TX) 12. St. John’s University Peter J. Tobin College of Business (Jamaica, NY) 13. Georgia Southern University College of Business Administration (Statesboro, GA) 14. Northeastern University D’Amore-McKim School of Business (Boston, MA) 15. DePaul University Kellstadt Graduate School of Business (Chicago, IL) 16. Golden Gate University Edward S. Ageno School of Business (San Francisco, CA) 17. Southern New Hampshire University College of Online and Continuing Education (Hooksett, NH) 18. California State University, Sacramento College of Business Administration (Sacramento, CA) 19. University of Scranton Kania School of Management (Scranton, PA) 20. Syracuse University Martin J. Whitman School of Management (Syracuse, NY) 21. University of Hartford Barney School of Business (West Hartford, CT) 22. University of Miami School of Business Administration (Coral Gables, FL) 23. George Mason University School of Business (Fairfax, VA) 24. University of South Dakota Beacom School of Business (Vermillion, SD) 25. Florida Atlantic University College of Business (Boca Raton, FL) 26. Stetson University M.E. Rinker Sr. Institute of Tax and Accountancy (DeLand, FL) 27. Rider University College of Business Administration (Lawrenceville, NJ) 28. New England College School of Graduate and Professional Studies (Henniker, NH) 29. Western Governors University (Salt Lake City, UT) 30. Indiana Wesleyan University DeVoe School of Business (Marion, IN) 31. Plymouth State University College of Business Administration (Plymouth, NH) 32. Bellevue University College of Business (Bellevue, NE) 33. Loyola University Chicago Quinlan School of Business (Chicago, IL) 34. Franklin University Ross College of Business (Columbus, OH) 35. Nova Southeastern University Huizenga College of Business (Fort Lauderdale, FL) 36. Saint Mary’s University Graduate School of Business and Technology (Winona, MN) 37. Baypath University School of Science & Management (Longmeadow, MA) *See the full rankings and program details here: http://www.mastersinaccounting.info/online-masters-in-accounting/ About MastersinAccounting.info: MastersinAccounting.info is a free online resource focused on providing accurate and up-to-date information on degrees, programs, and schools for prospective master’s in accounting students. The site also provides additional resources such as career outlooks, graduate student guides, scholarships, and more. MastersinAccounting.info’s goal is to be best in class.
News Article | February 15, 2017
Michael Solari, CFP®, has been named one of New Hampshire Union Leader’s 40 Under Forty recipients for 2017. Solari is a fee-only financial planner and owner of Solari Financial Planning. The Union Leader selects some of New Hampshire’s highest-achieving young professionals for its 40 Under Forty program. Winners are nominated by their peers for this distinction. Solari and other award winners will be honored at a reception in Concord on March 15. “I am honored that I was nominated and selected for the 2017 40 Under Forty program,” Solari said. “My mission is to help my clients take the guesswork out of building their wealth and reaching their goals.” Solari specializes in working with physicians to organize their finances while promoting a fiduciary standard. He has nearly a decade of experience creating comprehensive financial plans for families and built his own firm from scratch in 2013. Solari is also a member of the National Association of Personal Financial Advisors (NAPFA) and the XY Planning Network. You can view the full list of Union Leader’s 40 Under Forty recipients at unionleader.com. Solari Financial Planning is a fee-only financial planning firm with offices in Bedford, NH, Nashua, NH and Boston, MA. Owner Michael Solari, CFP®, has more than 10 years experience in personal financial planning. Contact Solari Financial Planning at Michael(at)SolariFinancial(dot)com or schedule a meeting at SolariFinancial.com.
News Article | February 24, 2017
MONTERREY, Mexico, Feb. 24, 2017 (GLOBE NEWSWIRE) -- Mexican airport operator Grupo Aeroportuario del Centro Norte, S.A.B. de C.V., known as OMA (NASDAQ:OMAB) (BMV:OMA), announces that the General Ordinary Shareholders’ Meeting held today approved the proprietary board members designated by OMA’s Strategic Partner, Servicios de Tecnología Aeroportuaria, S.A. de C.V. (SETA), other proprietary members of the board, and the board secretary. The composition of the board and board committees is as follows: About OMA Grupo Aeroportuario del Centro Norte, S.A.B. de C.V., known as OMA, operates 13 international airports in nine states of central and northern Mexico. OMA’s airports serve Monterrey, Mexico’s third largest metropolitan area, the tourist destinations of Acapulco, Mazatlán, and Zihuatanejo, and nine other regional centers and border cities. OMA also operates the NH Collection Hotel inside Terminal 2 of the Mexico City airport and the Hilton Garden Inn at the Monterrey airport. OMA employs over 1,000 persons in order to offer passengers and clients airport and commercial services in facilities that comply with all applicable international safety, security, and ISO 9001:2008 environmental standards. OMA is listed on the Mexican Stock Exchange (OMA) and on the NASDAQ Global Select Market (OMAB). For more information, visit:
News Article | February 8, 2017
MONTERREY, Mexico, Feb. 08, 2017 (GLOBE NEWSWIRE) -- Mexican airport operator Grupo Aeroportuario del Centro Norte, S.A.B. de C.V., known as OMA (NASDAQ:OMAB) (BMV:OMA), today published a call for an Ordinary Shareholders’ Meeting to be held on February 24, 2017. The translation of the full text of the call follows: The Board of Directors of Grupo Aeroportuario del Centro Norte, S.A.B. de C.V. (the “Company”), in compliance with articles 28, section IV and 42 of the Mexican Securities Law (“Ley del Mercado de Valores”), and in accordance with Articles 181, 183, 186, and 187 of the Mexican General Law of Corporations (“Ley General de Sociedades Mercantiles”) and articles Thirty Four, Thirty Five and Thirty Six of the Bylaws of the Company, hereby CALLS its shareholders to attend a General Ordinary Shareholders’ Meeting, which will be held starting at 9:00am on the 24th day of February, 2017, in Salon Regency Room G, of the Hyatt Regency Hotel, Campos Elíseos No. 204, Polanco Sección V, C.P. 11560, Mexico City, Mexico where the following matters will be attended: I. Discussion and, in the event, approval of a proposal to designate and/or ratify, as the case may be, members of the Board of Directors of the Company. Resolutions in this regard. II. Discussion and, in the event, approval of resolutions regarding the revocation of certain powers previously authorized by the Company, and, in the event, the authorization and/or ratification of powers to represent the Company. In order to be entitled to attend the Shareholders’ Meeting, shareholders shall obtain an entry pass issued and delivered by the Secretary of the Company at the address set forth below, starting the fourth business day prior to the meeting date, in accordance with the following terms: a. Shareholders must be registered in the Share Registry of the Company or validate the ownership of their shares or certificates pursuant to Articles 290 and 293 of the Mexican Securities Law. The Share Registry will be closed to new entries three days prior to the date of the Shareholders’ Meeting and on the day of said Meeting. b. Shareholders shall deposit their share certificates as provided in paragraph (a) above, at the offices of the Company set forth below, or at S.D. Indeval Institución para el Depósito de Valores, S.A. de C.V., or at any national or foreign banking institution, and present to the Company the deposit receipt issued by the respective institution for such purposes. c. Shareholders may attend the Shareholders’ Meeting in person or through authorized representatives, using a proxy form in accordance with Article 49, section III of the Mexican Securities Law, or any other method of representation authorized by law; therefore, the shareholders shall, as appropriate, in addition to the deposit receipt mentioned in paragraph (b) above, include the proxy form referred to herein. Said proxy form is available at the address set forth below. d. Brokerage firms and other financial institutions shall, for purposes of obtaining an entry pass, present a list that contains the name, address, and nationality of each shareholder and the number of shares represented, duly signed by the officer responsible for the preparation of the list. The share certificates duly deposited with the Secretary of the Board of Directors by the shareholders or their representatives for attendance purposes will be returned after the adjournment of the Meeting in exchange for the deposit receipts issued to the shareholders or their representatives. Please note that the proxy form, entry passes, and supporting documentation related to the matters listed in the Agenda shall be available to shareholders at the offices located at the Conference Room located on the 9th floor of the Torre Esmeralda II building at Boulevard Manuel Ávila Camacho No. 36, Lomas de Chapultepec, C.P. 11000, Mexico City, from the date of the publication of this Call for a Shareholders’ Meeting, from 9:00 AM to 2:00 PM and from 4:00 PM to 7:00 PM on working days. Mexico City, on the 8th day of February 2017 /s/ Diego Quintana Kawage Chairman of the Board of Directors About OMA Grupo Aeroportuario del Centro Norte, S.A.B. de C.V., known as OMA, operates 13 international airports in nine states of central and northern Mexico. OMA’s airports serve Monterrey, Mexico’s third largest metropolitan area, the tourist destinations of Acapulco, Mazatlán, and Zihuatanejo, and nine other regional centers and border cities. OMA also operates the NH Collection Hotel inside Terminal 2 of the Mexico City airport and the Hilton Garden Inn at the Monterrey airport. OMA employs over 1,000 persons in order to offer passengers and clients airport and commercial services in facilities that comply with all applicable international safety, security, and ISO 9001:2008 environmental standards. OMA is listed on the Mexican Stock Exchange (OMA) and on the NASDAQ Global Select Market (OMAB). For more information, visit:
News Article | March 1, 2017
CULVER CITY, Calif.--(BUSINESS WIRE)--NantHealth, Inc. (NASDAQ-GS: NH), a next-generation, evidence-based, personalized healthcare company, today announced that the company will present at the Cowen and Company 37th Annual Health Care Conference on Tuesday, March 7, 2017, at 9:20 a.m. ET at the Boston Marriott Copley Place hotel. An audio-only webcast of the presentation will be available at www.NantHealth.com. Listeners are encouraged to visit the web site at least 10 minutes prior to the start of the presentation to register, download and install any necessary software. The presentation will be archived and accessible on the web site for at least 90 days. NantHealth, Inc., a member of the NantWorks ecosystem of companies, is a next-generation, evidence-based, personalized healthcare company enabling improved patient outcomes and more effective treatment decisions for critical illnesses. NantHealth’s unique systems-based approach to personalized healthcare applies novel diagnostics tailored to the specific molecular profiles of patient tissues and integrates this molecular data in a clinical setting with large-scale, real-time biometric signal and phenotypic data to track patient outcomes and deliver precision medicine. For nearly a decade, NantHealth has developed an adaptive learning system, CLINICS, which includes its unique software, middleware and hardware systems infrastructure that collects, indexes, analyzes and interprets billions of molecular, clinical, operational and financial data points derived from novel and traditional sources, continuously improves decision-making and further optimizes our clinical pathways and decision algorithms over time. For more information please visit www.nanthealth.com and follow Dr. Soon-Shiong on Twitter @DrPatSoonShiong.
News Article | February 23, 2017
Registration is open for all of ALOHA Mind Math‘s interactive summer camp programs - from “STEMmer Camp,” a STEM (Science, Technology, Engineering & Math) summer camp for 10-14 year olds, to their half-day Summer Reading Camp for kids grades 1-5. Also available is their accelerated-pace Math, Reading | Writing camps for ages 5-12. Visit http://alohamindmath.com/summercamp-temp/ for more information. Summer Camps like those held at ALOHA learning centers in 19 states across the US help student combat Summer Slide – studies show that students who do not engage in summer learning actually score lower on standardized tests at the end of summer than they did at the beginning. ALOHA has created summer camps with an engaging learning format to keep children having fun, while they are learning key concepts. ALOHA has locations in AL, AR, AZ, CA, CT, FL, GA, IL, MA, MI, MO, NC, NH, NJ, NY, OH, PA, TX, VA. ALOHA Summer Reading Camp for grades 1 - 5: Helps children rediscover the joys of reading, while engaging in creative writing. Carefully selected grade level reading, often using popular children’s books, keeps things fun. The teacher-led exercises support common core reading & writing issues. Teachers also focus on improving vocabulary skills. ALOHA’s Math & Reading | Writing Accelerated Pace programs for ages 5 - 12: Instead of meeting once a week, the summer programs are held at an accelerated pace - 5 days a week; either half or full-day. This accelerated pace allows children to build on what they learned the day before and grow their skills and confidence in either Math or Reading | Writing. Both children who are high achievers and those needing extra help with these subjects, can benefit from ALOHA’s teacher-led camps. STEMmer Camp for 10-14 year olds: To interest older students across the U.S. in STEM (Science, Technology, Engineering & Math) careers, and help combat summer learning loss, ALOHA Mind Math added this STEM camp. According to studies, middle school is when children begin to form their career paths. This interactive gaming-based summer camp, combines the computer-based learning “missions” along with teacher-led projects. Registrations have already begun for all camps. For more information regarding ALOHA Summer Camps check with your local ALOHA center;* http://alohamindmath.com/summercamp-temp, or 877-256-4203. *Each ALOHA learning center is independently owned and operated; participation may vary. Contact a local center directly for details and to see if they are participating. For more information: Click here for info on the website, check the ALOHA Facebook page or call 877-256-4203 to find if a local center is participating in these programs. ALOHA Since 2006, ALOHA Mind Math, a leading provider of mental arithmetic and English Reading | Writing after school programs, has been guiding children in the U.S. between the ages of 3 through 14 to achieve academic excellence in these grades. ALOHA is currently training children at over 120 locations in 19 states across the U.S. Programs also include a Tiny Thinkers pre-school program for ages 3-5, and a STEM summer camp for 10-14 year-olds. The interactive learning process ALOHA uses enhances a child’s math, reading and writing capabilities. The teachers also assist children in developing skills and abilities such as observation and listening that result in the overall growth of the child. The ALOHA program is also in use in 20 countries worldwide. For more details on these unique programs please visit http://www.alohamindmath.com or search for the center closest to you by using our locator http://www.alohamindmath.com/locations.
News Article | February 21, 2017
PARSIPPANY, NJ--(Marketwired - Feb 21, 2017) - GAF, North America's largest roofing manufacturer, is proud to introduce a new non-halogen polyisocyanurate insulation: EnergyGuard™ NH Polyiso Insulation Board. With this product introduction, GAF is the first roofing manufacturer to offer a full line of Red List Free roofing assemblies across their asphaltic and single ply product lines. The development of GAF EnergyGuard™ NH Polyiso Insulation Board supports the company's commitment to providing architects, contractors, and building owners with affordable products that help them meet their sustainable and environmental design goals. EnergyGuard™ NH Polyiso Insulation Board has all the inherent properties and performance factors polyiso insulation is known for, including one of the highest insulation values and a UL Class A roofing fire rating, but does not contain any halogenated flame retardants. "There is increasing interest in Red List free products," said Jeanine K. Mulcahy, Product Manager -- Insulation and Fastener Systems for GAF. "The development of EnergyGuard™ NH Polyiso Insulation Board is one more indication that architects and contractors who share our commitment to sustainable design can count on GAF for environmentally preferable products." Transparent and Red-List Free Recognizing that numerous jurisdictions have enacted legislation restricting the use of one or more chemical flame retardants, GAF has taken the proactive step of replacing TCPP with a non-halogen material that offers the same flame-retardant properties. As a result, GAF EnergyGuard™ NH Polyiso Insulation Board is one of the few polyisocyanurate roofing solutions with a Declare label designated as Red List Free. "It's exciting to see this new Red List Free insulation available. We're seeing more and more product options available to project teams pursuing Living Building Challenge certification," said James Connelly, Director, Living Product Challenge at International Living Future Institute. GAF EnergyGuard™ polyiso is the company's premier line of insulation boards designed for use in practically any low-slope roofing application, including BUR, mod bit, and single-ply systems. EnergyGuard™ NH Polyiso Insulation Boards offers a high insulation value with an excellent "LTTR" value compared to any other FM Class I rated product of equivalent thickness. It also meets FM 4450/4470 and UL1256/790. About GAF: Founded in 1886, GAF is the largest roofing manufacturer in North America. The Company is an operating subsidiary of Standard Industries. GAF products include a comprehensive portfolio of steep-slope and commercial roofing systems, which are supported by an extensive national network of factory-certified contractors. Its success is driven by its commitment to Advanced Quality, Industry Expertise, and Solutions Made Simple. GAF was the first roofing manufacturer to offer a Lifetime limited warranty on all of its laminated shingles, which then evolved with the introduction of the GAF Lifetime Roofing System by extending the Lifetime coverage beyond just the roofing shingles. With a focus on social responsibility, GAF developed Advanced Protection® Shingle Technology, providing excellent durability and wind resistance while reducing the use of natural resources. The company also developed single-ply and asphaltic roofing membranes with excellent durability and high reflectivity to meet the most rigorous industry standards while helping commercial property owners and designers reduce energy consumption. GAF also supports the roofing industry through CARE, the Center for the Advancement of Roofing Excellence, which has provided education to nearly 200,000 professionals. CARE's mission is to help professional contractors and distributors build their businesses through sales and management education, and to provide product and installation training to contractors, distributors, architects, property owners, and related industry personnel. For more information about GAF, visit gaf.com. About Standard Industries: Standard Industries is a privately-held, global, diversified holding company with interests in building materials, aggregates, and related investment businesses in public equities and real estate. With over 7,500 employees and operations in more than 80 countries, Standard maintains a team-oriented culture of meritocracy, operational excellence, and a passionate focus on investing in its people.
News Article | February 15, 2017
A new strategy has been developed using peptides with amino and carboxylic functional groups as passivating ligands to produce methyl ammonium lead bromide (CH NH PbBr ) perovskite nanocrystals (PNCs) with excellent optical properties. The well-passivated PNCs can only be obtained when both amino and carboxylic groups are involved, and this is attributed to the protonation reaction between NH and COOH that is essential for successful passivation of the PNCs. To better understand this synergistic effect, peptides with different lengths have been studied and compared. Due to the polar nature of peptides, peptide-passivated PNCs (denoted as PNCs ) aggregate and precipitate from nonpolar toluene solvent, resulting in a high product yield (≈44%). Furthermore, the size of PNCs can be varied from ≈3.9 to 8.6 nm by adjusting the concentration of the peptide, resulting in tunable optical properties due to the quantum confinement effect. In addition, CsPbBr PNCs are also synthesized with peptides as capping ligands, further demonstrating the generality and versatility of this strategy, which is important for generating high quality PNCs for photonics applications including light-emitting diodes, optical sensing, and imaging.
News Article | February 15, 2017
Recently, organic–inorganic hybrid perovskite materials have drawn great attention for their outstanding performance in high-efficiency solar cells. Successful synthesis has been realized either in solution-based chemical deposition or vapor deposition. However, conflicts have never ceased among quality control, growth rate, process complexity, and instrument requirement, which have limited their development toward real applications. In this work, the first electrochemical fabrication of perovskite toward high-efficiency and scalable perovskite solar cells (PSCs) is established. The morphology and crystallization of the CH NH PbI film can be effectively controlled by simply modulating a few physical parameters. A detailed study on its optoelectronic properties reveals significantly improved film quality and interfacial conditions. Aided by this, the total process does not require standard annealing, which greatly reduces the total growth time from hours to minutes. Up to now, an efficiency of 15.65% has been achieved in planar PSCs under 1 sun AM 1.5 condition, with small hysteresis and efficiency loss under longtime exposure to air. Moreover, high efficiency (10.45%) can be easily attained for large cells (2 cm2). This result will hopefully facilitate research for applicable high-efficiency PSCs and other multicomponent materials as well.