NGSM Institute of Pharmaceutical science
NGSM Institute of Pharmaceutical science
Mathew J.,NGSM Institute of Pharmaceutical science |
Joshi Chintankumar K.,NGSM Institute of Pharmaceutical science |
Jonils A.,NGSM Institute of Pharmaceutical science
International Journal of Research in Pharmaceutical Sciences | Year: 2011
Four simple, sensitive, rapid and accurate analytical methods have been developed for the estimation of paliperi-done in bulk and pharmaceutical dosage forms. Paliperidone is an atypical antipsychotic. Methnd I was a precise reverse phase HPLC involving an isocratic elution of Paliperidone in a column of Pkenomenex CIS, using a mobile phase composition of acetonitrile: Methanol: Potassium dihydrogen phosphate (45:30:25, v/v). The flow rate was 1.0 ml/min and the effluent was monitored at 275 nm and retention time was observed at 4.15 mm. Forced de-gradation studies were carried out in acid and basic medium, oxidative degradation in H2O2 and as well as uv pho-to stability studies. Methnd H & HI are visible spectrophotometnc methods based on the formation of red colored complex between Paliperidone and PDACA which showed linearity in the concentration range 20-120 ig/ml and between Paliperidone and 4-Amino phenazone in red colored chromogen which obeyed Beer's law 30-150 ig/ml at absorbance maxima of 460 nm and 532 nm respectively Method IV was a simple ultraviolet spectroscopic me-thod was developed for the estimation of Paliperidone in 0.1N HC1 which obeyed Beer's law in the linearity range 5-25 ig/ml at 275 nm. The proposed methods are optimized and validated as per the ICH guidelines. Recovery of paliperidone in the proposed methods was found to be in the range of 97.5-99.2 %. The proposed methods can be used for routine analysis for the estimation of paliperidone in formulations. © JK Welfare & Pharmascope Foundation.
Fernandes J.,NGSM Institute of Pharmaceutical science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017
Objective: Plants have been one of the important sources of medicines since the beginning of human civilization. Achyranthes aspera Linn. belonging to family Amaranthaceae, commonly found in India. The plant is used as antiarthritic, purgative, diuretic, antimalarial, etc. Methods: The antihyperlipidemic activity of alcoholic and aqueous extracts of leaves of A. aspera was evaluated using triton-induced hyperlipidemia model in albino Wistar rats. The acute toxicity study confirmed that both the extracts were found to be safe at 5000 mg/kg b.w. in female albino Wistar rats. In this model, eight groups (six animals per group) of animals were used. Group I and II were considered as saline and hyperlipidemic controls, respectively, and other groups received their respective agents (alcoholic or aqueous extract/atorvastatin/ayurvedic preparation) and all the groups received triton WR1339 (200 mg/kg b.w. i.v.) except saline control group. Serum cholesterol and triglycerides (TGs) level are estimated for the evaluation of antihyperlipidemic effects of extracts. Results: The ethanolic extract has shown a significant reduction in serum cholesterol and TGs level indicating antihyperlipidemic potentials in the plant while aqueous extract has not shown any beneficial effect. Conclusion: In this triton-induced hyperlipidemia model, serum and TG levels were analyzed ethanolic extract was shown antihyperlipidemic activity. Further clinical studies are compulsory to confirm the findings. © 2017 The Authors.
Bhat K.I.,NGSM institute of pharmaceutical science
International Journal of Pharmaceutical Sciences Review and Research | Year: 2011
This study is to evaluate the in vitro cytotoxic effect of petroleum ether and ethanolic flower extracts of Clitoria ternatea Linn by using trypan blue dye exclusion method. Both extracts exhibit significant cell cytotoxic activity. Phytochemical screening of petroleum ether extract reveals the presence of steroids, triterpinoids, tannins, saponins, while in ethanolic extract shows the presence of flavanoids.
Dharbhamulla S.,NGSM Institute of Pharmaceutical Science
European Journal of Medicinal Chemistry | Year: 2010
A series of 7-(2-substituted phenylthiazolidinyl)-benzopyran-2-one derivatives have been synthesized by reaction of 7-amino-4-methyl-benzopyran-2-one (1) with an appropriate substituted aldehydes to obtain various Schiff bases (3a-k) which on treatment with thioglycolic acid afforded the title compounds (4a-k). Purity of the compounds has been confirmed by TLC. Structure of these compounds were established on the bases IR, 1H NMR, 13C NMR and Mass spectral data. Schiff bases and title compounds were evaluated for antibacterial and antifungal activities against various bacterial and fungal strains. The results showed that compounds 3d, 3f, 4d, 4f and 4i (100 μg/ml) exhibited good antibacterial and antifungal activity as that of standard antibiotics Ciprofloxacin and Griseofulvin. © 2009 Elsevier Masson SAS. All rights reserved.
Ishwar B.K.,NGSM institute of pharmaceutical science
International Journal of ChemTech Research | Year: 2010
A series of aminobenzylated mannich bases of pyrazinamide (2a to 2j) have been prepared by mannich reaction of aromatic aldehydes with pyrazinamide and secondary amines. The chemical structure of all the synthesized compounds have been elucidated by spectral studies (IR, H1 NMR). Also they have been assayed in vitro for their biological activity against E.coli, B.substilis, S.aureas bacterial species and A. niger and C. albicans fungal micro organisms.
Shastry C.S.,NGSM Institute of Pharmaceutical science |
Shafeeque A.A.,NGSM Institute of Pharmaceutical science |
Ashwathnarayana B.J.,NGSM Institute of Pharmaceutical science
Indian Journal of Pharmacology | Year: 2013
Objectives: Aripiprazole, a new atypical antipsychotic drug extensively metabolized by enzyme CYP3A4, is found to produce asymptomatic elevation of serum transaminase levels on long-term treatment. The present study aims to evaluate the hepatotoxic effect of aripiprazole when coprescribed with carbamazepine and fluvoxamine. Materials and Methods: The rats were subjected to chronic treatment with two different doses, therapeutic dose (TD) and maximum therapeutic dose (MTD), of aripiprazole in combination with carbamazepine and fluvoxamine. The changes in hepatic function was assessed by various biochemical liver enzyme markers like serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin, histological studies, and physical parameters (liver weight, liver volume, and body weight). Results: The combination of aripiprazole with fluvoxamine at both TD and MTD showed the hepatic damage and significant elevation in serum transaminase level which is supported by histological reports. The coadministration of aripiprazole with carbamazepine leads to significant decrease in blood concentration of aripiprazole possibly due to induction of enzyme CYP3A4 resulting in loss or reduction of clinical efficacy. Conclusions: There would be an accumulation of aripiprazole when coadministered with fluvoxamine, a known inhibitor of CYP3A4, leading to hepatic damage and reduction in aripiprazole when administered along with carbamazepine. Therefore, aripiprazole with fluvoxamine and carbamazepine should be coprescribed with caution. The patients should be monitored for signs of adverse effects like hepatic damage or decreased efficacy of these drugs.
Anupama N.,Vellore Institute of Technology |
Madhumitha G.,Vellore Institute of Technology |
Rajesh K.S.,NGSM Institute of Pharmaceutical Science
BioMed Research International | Year: 2014
Inflammation plays an important role in various diseases with high prevalence within populations such as rheumatoid arthritis, ulcer, atherosclerosis, and asthma. Many drugs are available in the market for inflammatory diseases. They exhibit several unwanted side effects to humans. Therefore, alternative treatments with safer compounds are needed. Carissa carandas plant is used in traditional medicinal system for its various diseases curing property. In the present study, we examined the anti-inflammatory effects of dried fruit methanol extract on carrageenan-induced hind paw edema in rats. C. carandas was defatted with petroleum ether, followed by methanol extraction. The methanol extracts of the dried fruits of Carissa carandas were given orally to the experimental rats caused significant activity (P ≤ 0.05) when compared with the control group. The maximum inhibition of paw edema was found to be in Group V, that is, 76.12% with inhibition of paw volume in a dose-dependent manner. The anti-inflammatory activity of the methanol extract of the dried fruits shows that the presence of potential constituents present in this extract may provide assistance in the drug discovery process. The phytochemical compounds of the extract were screened by GC-MS analysis and it was found that 11 compounds are present in methanol extract of dried fruits of Carissa carandas. © 2014 N. Anupama et al.
Girisha K.S.,Mangalore University |
Kalluraya B.,Mangalore University |
Narayana V.,NGSM Institute of Pharmaceutical science |
Padmashree,NGSM Institute of Pharmaceutical science
European Journal of Medicinal Chemistry | Year: 2010
A series of 1-acetyl/propyl-3-aryl-5-(5-chloro-3-methyl-1-phenyl-1H- pyrazol-4-yl)-2-pyrazolines were synthesized in one step by condensing suitably substituted propenones, hydrazine and acetic/propionic acid. The newly synthesized pyrazolines were characterized by analytical and spectral data. The new compounds were screened for analgesic and anti-inflammatory activity and most of them showed good activity comparable with that of standard drugs Pentazocin and Diclofinac sodium respectively. © 2010 Elsevier Masson SAS. All rights reserved.
Nisha Shetty G.,NGSM Institute of Pharmaceutical science |
Charyulu R.N.,NGSM Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2013
This work describes the stability of the selected in situ solutions for ophthalmic delivery of naphazoline hydrochloride (FF17, FF18) and antazoline phosphate (GG17 and GG18) based on the pH triggered concept using Carbopol 940 and HPMC K4M. The formulations were evaluated for their pH, isotonicity, gelling capacity,rheological characteristics, in vitro drug release, sterility and in vivo studies in New Zealand rabbits' eyes. All the formulations showed satisfactory results at ocular pH environment that remains in contact with the eyes for few hours. The formulations were very stable throughout, at room temperature and at 40 °C. Higher amount of both the drugs were retained in the aqueous humour area over 8 hrs following instillation FF17, FF18, GG17& GG18. Therefore, in situ gelling system can be used to enhance the ocular retention time thereby increasing ocular bioavailability and reducing the frequency of dosing.
Hunashal R.D.,Karnataka Institute of Medical science |
Satyanarayana D.,NGSM Institute of Pharmaceutical science
International Journal of Pharma and Bio Sciences | Year: 2012
The reaction of thiocarbohydrazide with substitutedphenoxy acetic acid to obtained 3-substituted-4-amino-5-mercapto-1,2,4-triazoles (1) by condensing compound 1 with aromatic carboxylic acid and substituted phenoxy acetic acids resulted the title compound triazolothiadiazoles 2a-j. The purity of the newly synthesized compounds was confirmed by TLC. Structures of all the newly synthesized compounds were confirmed by spectral data. All the newly synthesized compounds were screened for their in-vitroantimicrobial activity.Among the series the compounds 2e, 2f and 2b, 2c, 2f, 2d, 2i were exhibited equipotent antibacterial and antifungal activity a MIC of 1 μg/mL when compared with standard drugs respectively. From the results the compounds 2c, 2f, 2j were showed comparable antitubercular activity againstM. tuberculosisH37Rv at MIC of 0.50 μg/mL, when compared with standard drug Rifampin and INH which showed MIC of 0.25 μg/mL.