New England Biolabs | Date: 2017-02-01
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.
Correa I.R.,New England Biolabs
Current Opinion in Chemical Biology | Year: 2014
Advances in the development of new fluorescent reporters and imaging techniques have revolutionized our ability to directly visualize biological processes in living systems. Real-time analysis of protein localization, dynamics, and interactions has been made possible by site-specific protein labeling with custom designed probes. This review outlines some of the most recent advances in the design and application of live-cell imaging probes, with a particular focus on SNAP-tag technology. Specific examples illustrating applications in superresolution and single-molecule imaging, protein trafficking and recycling, and protein-protein interactions are presented. © 2014 Elsevier Ltd.
New England Biolabs | Date: 2015-02-11
Novel proteins having DNA polymerase are described which have utility in amplification reactions and have improved properties over Bst polymerase such as for example enhanced reverse transcriptase activity.
New England Biolabs | Date: 2016-04-19
Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
New England Biolabs | Date: 2015-10-29
Nucleic acids comprising -glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.
New England Biolabs | Date: 2015-08-27
This disclosure provides, among other things, a composition comprising: a 5 exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
New England Biolabs | Date: 2015-08-28
Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.
New England Biolabs | Date: 2016-01-12
Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
New England Biolabs | Date: 2015-07-13
Compositions and methods are provided for inhibiting a DNA binding enzyme from reacting with non-target DNA at a temperature below the reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but may fold to form at least one double stranded region designed to melt at a temperature which is lower than the reaction temperature, and at least one single stranded region where the single stranded region at the 5 end contains at least one unnatural and/or modified nucleotide and optionally a sequence at the 3 end contains one or more derivative nucleotide or linkages.
New England Biolabs | Date: 2016-02-17
This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.