Neurotar | Date: 2016-05-17
Optical inspection apparatus and devices, namely, medical imaging apparatus for small animals, in a research laboratory environment. Scientific and technological services, namely, scientific research and testing in the field of brain diseases; research in the field of brain diseases; design of imaging devices and apparatus for medical and diagnostic use in the field of brain research.
Neurotar | Date: 2014-08-15
Neurotar | Date: 2015-09-03
Lihavainen E.,Tampere University of Technology |
Kislin M.,University of Helsinki |
Toptunov D.,Neurotar |
Khiroug L.,University of Helsinki |
Ribeiro A.S.,Tampere University of Technology
Journal of Microscopy | Year: 2015
The morphology of mitochondria can inform about their functional state and, thus, about cell vitality. For example, fragmentation of the mitochondrial network is associated with many diseases. Recent advances in neuronal imaging have enabled the observation of mitochondria in live brains for long periods of time, enabling the study of their dynamics in animal models of diseases. To aid these studies, we developed an automatic method, based on supervised learning, for quantifying the degree of mitochondrial fragmentation in tissue images acquired via two-photon microscopy from transgenic mice, which exclusively express Enhanced cyan fluorescent protein (ECFP) under Thy1 promoter, targeted to the mitochondrial matrix in subpopulations of neurons. We tested the method on images prior to and after cardiac arrest, and found it to be sensitive to significant changes in mitochondrial morphology because of the arrest. We conclude that the method is useful in detecting morphological abnormalities in mitochondria and, likely, in other subcellular structures as well. © 2015 Royal Microscopical Society.
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Posted on May 26, 2016. Blood-Brain Barrier Conference (June 15-16) is part of the World Preclinical Congress taking place June 15-17, 2016 in Boston, MA ...