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Gu P.,Hebei Medical University | Gu P.,Neuroscience Laboratory of Hebei Province | Qiu F.-C.,Hebei Medical University | Han R.,Neuroscience Laboratory of Hebei Province | And 6 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Neural stem cells (NSCs) are valuable self-renewing cells that can maintain the capacity to differentiate into specific brain cell types. NSCs may repair and even replace the brain tissue, and ultimatley promoting the central nervous system regeneration. Therefore, it is important, for scientists and pjysicians, to study the method for efficient culture and differentiation of NSCs. Our previous study demonstrated that Bone Marrow Stromal Cells (BMSCs) can directly regulate the differentiation of NSCs into neurons, and soluble molecules excreted by BMSCs played a key role in this process. Hereby, we further identified the BMSCs-induced neurons could form the synapses, convey dopamine and express voltage-depend and receptor-depend calcium channels. Moreover, the extracellular signal-regulated protein kinase ERK1/2 pathway was founded to be involved in the process of neuron differentiation and proliferation by the in vitro experiments. Finally, by using protein array, we, for the first time, found that the cytokine-induced neutrophil chemoattractant-3 (CINC-3, a small molecule cytokine) can promote the leukocytes invasion into the inflammation site, and have the ability to induce mesencephal NSCs into neurons. Consequently, these positive findings suggested that our BMSCs-induced culture system could provide a useful tool to investigate the molecular mechanisms of neural differentiation of NSCs, which may be benifical for neurodegenerative diseases in the near future. © 2015 E-Century Publishing Corporation. All rights reserved.

Xie B.,Hebei Medical University | Gu P.,Hebei Medical University | Gu P.,Neuroscience Laboratory of Hebei Province | Wang W.,Hebei Medical University | And 8 more authors.
American Journal of Translational Research | Year: 2016

Objective: Human umbilical cord mesenchymal stem cells (hUC-MSCs) hold substantial promise for the treatment of ischemic neurological disease, but few clinical data are currently available about its therapeutic effects in hypoxic ischemic encephalopathy (HIE). This study is to evaluate the effects of hUC-MSCs transplantation on patients with HIE. Methods A total 22 patients with HIEwere randomly divided into hUC-MSCs transplantation group (n = 12) and control group (n = 10). After isolation, hUC-MSCs were cultured for 3 to 5 passages in vitro and then intravenously administered to HIE patients in the transplantation group, while the control group received routine treatment only. The outcomes of HIE patients were evaluated at designated time points by clinical assessment scales, including NIHSS, Barthel Index, MMSE, HAMA24, HAMD14 and UPDRS. Results: hUC-MSCs were identified by morphological analysis and flow cytometry assays before clinic transplantation. No significant differences of demographic characteristics were observed between the two groups of subjects. Compared to the control group, hUC-MSCs transplantation markedly improved the outcomes of HIE patients leading to better recovery of neurological function, cognition ability, emotional reaction and extrapyramidal function. No significant adverse effects were found in subjects with hUC-MSCs transplantation during a 180-day follow-up period. Conclusion: These data suggest that hUC-MSCs therapy markedly improves the outcomes of patients with HIE, which is potential for the routine treatment of ischemic neurological disease. © 2016, E-Century Publishing Corporation. All rights reserved.

Wang W.-T.,Hebei Medical University | Gu P.,Hebei Medical University | Gu P.,Neuroscience Laboratory of Hebei Province | Qiu F.-C.,Hebei Medical University | And 7 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2016

Objective: Comparative analysis the effects of subarachnoid transplantation of autologous bone marrow mesenchymal stem cells (BM-MSC) and intravenous transplantation of allograft human umbilical cord mesenchymal stem cells (hUC-MSC) on patients with Parkinson’s syndrome (PS). Methods: Patients with PS who had been definitely diagnosed and hospitalized, eithersubarachnoid transplantation of autologous BM-MSC or intravenous transplantation of allograft hUC-MSC, were included in our hospital. Patients were graded according to the following scales prior to and three months after transplantation: (1) Unified Parkinson’s Disease Rating Scale (UPDRS); (2) International Cooperative Ataxia Rating Scale (ICARS); (3) Activities of Daily Living Scale (ADL); (4) Minimental state examination (MMSE); (5) Hamilton Depression Scale 24 Item (HAMD24) and Hamilton Anxiety Scale 14 Item (HAMA14 to evaluate the symptoms of PS, degree of ataxia, quality of life, cognitive functions and emotional changes. Results: BM-MSC transplantation was performed in seven patients, four of which significantly improved, 2 patients improved slightly, and one had no improvement.The group receiving hUC-MSCs included 5 patients,3 of which significantly improved, 1 was slightly improved and one had no improvement. Comparing the symptoms before and three months after transplantation,we observed that the UPDRS total score, UPDRSII, UPDRSIII, ICARS scores of gait, cooperative ataxia, dysarthrosis were significantly decreased (P<0.05), and ADL scores were significantlyincreased (P<0.05) in both groups.Most importantly,the hUC-MSC group showed improvements in the scores of UPDRSI, HAMA14 and HAMD24, the ICARS scores of ocular duskiness were significantly decreased (all P<0.05), while the MMSE score was significantly increased (P<0.05). However, these scores were not significantly changed in the BM-MSC group. Conclusion: The clinical symptoms (ataxia, language and swallowing) of PS patients were improved in both subarachnoid transplantation of autologous BM-MSC and intravenous transplantation of allograft hUC-MSC. The hUC-MSC transplantation, but not BM-MSC transplantation, significantly improved the cognition and emotional function of these patients. © 2016 American Scientific Publishers All rights reserved.

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