Foundation Neurological Institute Irccs C Besta

Milano, Italy

Foundation Neurological Institute Irccs C Besta

Milano, Italy
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Musio S.,Foundation Neurological Institute Irccs C Besta | Costanza M.,Foundation Neurological Institute Irccs C Besta | Poliani P.L.,University of Brescia | Fontana E.,University of Brescia | And 4 more authors.
Neurology: Neuroimmunology and NeuroInflammation | Year: 2017

Objective: To investigate the effects of targeting the high-affinity receptor for immunoglobulin E (FcϵRI), that plays a central role in allergic responses and is constitutively expressed on mast cells and basophils, in clinical disease and autoimmune T-cell response in experimental MS. Methods: Experimental autoimmune encephalomyelitis (EAE) was induced in C57BL/6 mice by immunization with myelin oligodendrocyte glycoprotein 35-55. Anti-FcϵRI α-chain antibody was administered intraperitoneally. CNS immunohistochemistry, flow cytometry analysis of immune cell populations, IgE and histamine serum concentration, immune cell proliferation, and cytokine measurement were performed. In BALB/c mice, EAE was induced by immunization with myelin proteolipid protein 185-206. Results: Treatment with anti-FcϵRIα antibody resulted in exacerbation of EAE and increased CNS inflammation in C57BL/6 mice. Treated mice displayed long-lasting complete depletion of basophils in the blood stream and peripheral lymphoid organs and increased antigeninduced immune cell proliferation and production of interferon-g, interleukin (IL)-17, IL-6, and granulocyte-macrophage colony-stimulating factor. In BALB/c mice, which are T-helper (Th) 2 prone and resistant to EAE, treatment with anti-FcϵRIαa antibody restored susceptibility to EAE. Conclusion: Our observations that anti-FcϵRIα antibody increases Th1 and Th17 responses against myelin antigen and exacerbates EAE suggest that FcϵRI, basophils, and possibly other FcϵRI-bearing cells that might be affected by this antibody play important roles in influencing the severity of CNS autoimmunity. Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

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