Time filter

Source Type

Agostini S.,Neuro Gastroenterology and Nutrition Unit | Goubern M.,Danone Research Center Carasso | Tondereau V.,Neuro Gastroenterology and Nutrition Unit | Salvador-Cartier C.,Neuro Gastroenterology and Nutrition Unit | And 9 more authors.
Neurogastroenterology and Motility | Year: 2012

Background Fermented milk (FM) containing Bifidobacterium lactis CNCM I-2494 and yogurt strains improves irritable bowel syndrome (IBS) symptoms in constipated IBS patients. In rats, stressful events exacerbate IBS symptoms and result in the alteration of gut sensitivity and permeability via epithelial cell cytoskeleton contraction. In a stress model, we aimed at evaluating the effect of B. lactis CNCM I-2494 as a pure strain or contained in an FM product on visceral sensitivity and the impact of this FM on intestinal barrier integrity. Methods Visceral sensitivity was analyzed in rats subjected to partial restraint stress (PRS). Rats received during 15days the B. lactis as a pure strain (10 6 to 10 10CFUmL -1), B. lactis in an FM product (10 8CFUg -1, diluted or not), or a control product. Gut paracellular permeability, colonic occluding and Jam-A proteins, and blood endotoxin levels were determined in rats receiving B. lactis in an FM product submitted or not to a PRS. Key Results The FM product showed a dose-dependent inhibitory effect on stress-induced visceral hypersensitivity. A similar antihyperalgesic effect was observed at 10 10CFUmL -1 of pure B. lactis administration. The FM product prevented the increase in intestinal permeability induced by PRS and restored occludin and JAM-A expressions to control levels. The FM product abolished the increase concentration of blood endotoxin induced by PRS. Conclusions & Inferences This study illustrates that a probiotic food containing B. lactis CNCM I-2494 strain reduces visceral hypersensitivity associated with acute stress by normalizing intestinal epithelial barrier via a synergistic interplay with the different probiotic strains and/or metabolites contained in this product. © 2012 Blackwell Publishing Ltd.

Loading Neuro Gastroenterology and Nutrition Unit collaborators
Loading Neuro Gastroenterology and Nutrition Unit collaborators