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Abbandonato G.,NEST | Storti B.,NEST | Signore G.,Center for Nanotechnology Innovation at | Beltram F.,NEST | Bizzarri R.,NEST
Microscopy Research and Technique | Year: 2016

Reversible photoswitching has been proposed as a way to identify molecules that are present in small numbers over a large, non-switching, background. This approach, called optical-lock-in-detection (OLID) requires the deterministic control of the fluorescence of a photochromic emitter through optical modulation between a bright (on) and a dark state (off). OLID yields a high-contrast map where the switching molecules are pinpointed, but the fractional intensities of the emitters are not returned. The present work presents a modified OLID approach (quantitative OLID or qOLID) that yields quantitative information of the switching (fSW) and non-switching (fNS) components. After the validation of the method with a sample dataset and image sequence, we apply qOLID to measurements in cells that transiently express the photochromic protein EYQ1. We show that qOLID is efficient in separating the modulated from the non-modulated signal, the latter deriving from background/autofluorescence or fluorophores emitting in the same spectral region. Finally, we apply qOLID to Förster (Fluorescence) Resonance Energy Transfer (FRET) imaging. We here demonstrate that qOLID is able to highlight the distribution of FRET intensity in a sample by using a photochromic donor and a non-photochromic acceptor. © 2016 Wiley Periodicals, Inc.


Sreekumar A.,NeST | Meena A.,NeST | Shahabudeen S.,NeST
SAE International Journal of Passenger Cars - Electronic and Electrical Systems | Year: 2014

This paper presents an overview of the telematics domain, with specific focus on the Indian Market. Telematics service areas broadly includes V2X (vehicle-to-vehicle and vehicle-to-infrastructure) and safety communications, fleet management and monitoring, vehicle security, diagnostics, emergency and roadside assistance, automatic crash notification, service appointment scheduling, toll collection, vehicle insurance. Navigation, infotainment and in-car communications are also closely related areas. An emerging trend is that V2X telematics may be delivered using smartphones and networks such as 4G. The primary reason is cost advantage in utilizing smartphones as the platform to bring services to vehicles. Smartphone based applications and web portals can be used to enable remote access features. In fact, if Federal Communications Commission (FCC) decides to eventually share the 5.9 GHz DSRC band, telematics may in fact rely much more on standard telecommunications infrastructure. Thus, a good strategy in the near future may be to reduce reliance on DSRC equipment in vehicles and use smartphones and standard 4G and other telecommunication infrastructure to provide useful telematics services, especially for emerging markets such as India. For India, where road and vehicle safety are major issues and opportunities for customized value added services abound, a detailed analysis of the trends in telematics is required. In terms of telematics services, India is significantly behind other BRICS countries such as China and Brazil. This paper presents some insights into technologies and services that are likely to take off in India in the near future. A user survey has been conducted to gauge the market preferences for Telematics services in India. © 2014 SAE International.


News Article | November 28, 2016
Site: www.prnewswire.com

WASHINGTON, Nov. 28, 2016 /PRNewswire/ -- As Katrina made landfall in the hurricane season of 2005 - emergency managers evacuating New Orleans, faced their biggest challenge - determining where, when, and who would be harmed the most. Photo -...


News Article | February 28, 2017
Site: www.businesswire.com

LINCOLN, Neb.--(BUSINESS WIRE)--Two of the Nebraska Education Savings Trust (NEST) college savings plans—NEST Advisor College Savings Plan and TD Ameritrade 529 College Savings Plan—have each surpassed $1 billion in assets, as of February 17. Administered by Nebraska State Treasurer Don Stenberg and managed by First National Bank of Omaha, total assets in all four NEST plans exceed $4.3 billion. “This is a remarkable achievement for NEST as it reflects our hard work on behalf of the families who place their financial trust in us,” said Treasurer Stenberg, NEST Trustee. “Our college savings plans have a lot to offer both in terms of value and variety, and providing strong performing plans with over $1 billion in assets under management is something we are very proud of.” In 2016, both the NEST Advisor Plan and TD Ameritrade 529 College Savings Plan lowered their fees and made adjustments to their underlying funds, offering more savings options. NEST plans have earned high marks and received accolades from industry rating sites Morningstar and SavingforCollege.com. “With nearly a quarter of a million accounts open, NEST prides itself on providing robust savings programs for our investors,” said Deborah Goodkin, Managing Director, Savings Plans, First National Bank of Omaha. “As two of our plans each break the $1 billion benchmark, it’s clear that families across the country see the benefit of investing with NEST to grow their college savings nest eggs.” In addition to the NEST Advisor College Savings Plan and the TD Ameritrade 529 College Savings Plan, NEST offers the NEST Direct College Savings Plan and The State Farm College Savings Plan. The variety of investment options offered by NEST allows families to choose the plan that best fits the ages of their children, their budgets and their investment philosophies. For more information about NEST, visit NEST529.com and treasurer.nebraska.gov. NEST is a tax-advantaged 529 college savings plan and provides four plans to help make saving for college simple and affordable: NEST Direct College Savings Plan, the NEST Advisor College Savings Plan, the TD Ameritrade 529 College Savings Plan, and The State Farm College Savings Plan. The Nebraska State Treasurer serves as Program Trustee. First National Bank serves as the Program Manager, and all investments are approved by the Nebraska Investment Council. Families nationwide are saving for college using Nebraska's 529 College Savings Plans, which have more than 249,000 accounts, including 75,000 in Nebraska. Visit NEST529.com and treasurer.nebraska.gov for more information. About First National Bank of Omaha First National Bank of Omaha is a subsidiary of First National of Nebraska. First National of Nebraska is the largest privately owned banking company in the United States. First National and its affiliates have $20 billion in managed assets and 5,000 employee associates. Primary banking offices are located in Nebraska, Colorado, Illinois, Iowa, Kansas, South Dakota and Texas. Investments Are Not FDIC Insured* - No Bank, State or Federal Guarantee - May Lose Value *Except the Bank Savings Individual Investment Option

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