Neogen Europe Ltd

Ayr, United Kingdom

Neogen Europe Ltd

Ayr, United Kingdom
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Johnson P.E.,University of Manchester | Johnson P.E.,UK Institute of Food Research | Rigby N.M.,UK Institute of Food Research | Dainty J.R.,UK Institute of Food Research | And 27 more authors.
Food Chemistry | Year: 2014

A dessert matrix previously used for diagnosis of food allergies was incurred with pasteurised egg white or skimmed milk powder at 3, 6, 15 and 30 mg allergen protein per kg of dessert matrix and evaluated as a quality control material for allergen analysis in a multi-laboratory trial. Analysis was performed by immunoassay using five kits each for egg and milk (based on casein) and six 'other' milk kits (five based on β-lactoglobulin and one total milk). All kits detected allergen protein at the 3 mg kg-1 level. Based on ISO criteria only one egg kit accurately determined egg protein at 3 mg kg-1 (p = 0.62) and one milk (casein) kit accurately determined milk at 6 (p = 0.54) and 15 mg kg-1 (p = 0.83), against the target value. The milk "other" kits performed least well of all the kits assessed, giving the least precise analyses. The incurred dessert material had the characteristics required for a quality control material for allergen analysis. © 2013 Elsevier Ltd. All rights reserved.


Grant
Agency: European Commission | Branch: FP7 | Program: BSG-SME | Phase: SME-1 | Award Amount: 1.44M | Year: 2008

Under the current issues surrounding climate change, conserving water resources is becoming an increasing priority. As areas become stressed due to water exploitation or environmental pressures, the amount of water resources available for use have been decreasing (World Meteorological Organisation, 1999) and Europe has not been able to avoid these pressures. It is crucial to protect and improve water consumption to provide a sustainable practice for economic development and to maintain human settlements. LOWTEV will bring considerable water savings to the food industry which is comprised of over 230,000 SME organisations. With an excellent consortium with experts in the fields of food science and sanitisation we will develop a low water pressure ultrasound device integrated with an automated rapid hygiene monitoring system. By providing an alternative system for cleaning in place technology on bulk handling equipment for food processors, a factory site would benefit from at least a 10% savings in overall water use. This equates to an estimated 30,000 worth of savings on water and energy bills per factory, providing a return on investment in under 1 year of installation. The food industry sector within Europe lag behind in terms of innovation and R &D investment; this project will address the environmental issues to improve water resources and will also improve the competitiveness of the SME food processing and bulk handling communities. Through reducing the clean cycle times on conveyor equipment, this will realise more productivity within the food processing cycle by reducing the labour costs and times for cleaning.


Grant
Agency: European Commission | Branch: FP7 | Program: CP | Phase: SME-2011-3 | Award Amount: 1.16M | Year: 2012

LOWTEV will bring considerable water savings to the food processing industry which is comprised of over 300,000 SME organisations. With an excellent consortium including experts in the fields of food processing and ultrasound sanitisation we will demonstrate our ultrasonic Clean In Place (CIP) technology for use in the food processing environment. Our technology was developed during the FP7 R4SME Project LOWTEV (Grant agreement no. 222078) and has shown 60% savings in cleaning water consumption 30% savings in energy consumption 20% increase in productivity during proof of concept trials in the laboratory. Using our LOWTEV CIP technology on bulk handling and conveyance equipment for food processors, a factory site would benefit from 60-80% savings in cleaning water consumption and at least a 10% savings in overall water use. This equates to an estimated 30,000 worth of savings on water and energy bills per factory per annum, providing a return on investment in 1-2 years of installation.


PubMed | Queen's University of Belfast, SNeogen Corporation and Neogen Europe Ltd
Type: Journal Article | Journal: Analytical chemistry | Year: 2015

A single-step lateral flow immunoassay (LFIA) was developed and validated for the rapid screening of paralytic shellfish toxins (PSTs) from a variety of shellfish species, at concentrations relevant to regulatory limits of 800 g STX-diHCl equivalents/kg shellfish meat. A simple aqueous extraction protocol was performed within several minutes from sample homogenate. The qualitative result was generated after a 5 min run time using a portable reader which removed subjectivity from data interpretation. The test was designed to generate noncompliant results with samples containing approximately 800 g of STX-diHCl/kg. The cross-reactivities in relation to STX, expressed as mean SD, were as follows: NEO: 128.9% 29%; GTX1&4: 5.7% 1.5%; GTX2&3: 23.4% 10.4%; dcSTX: 55.6% 10.9%; dcNEO: 28.0% 8.9%; dcGTX2&3: 8.3% 2.7%; C1&C2: 3.1% 1.2%; GTX5: 23.3% 14.4% (n = 5 LFIA lots). There were no indications of matrix effects from the different samples evaluated (mussels, scallops, oysters, clams, cockles) nor interference from other shellfish toxins (domoic acid, okadaic acid group). Naturally contaminated sample evaluations showed no false negative results were generated from a variety of different samples and profiles (n = 23), in comparison to reference methods (MBA method 959.08, LC-FD method 2005.06). External laboratory evaluations of naturally contaminated samples (n = 39) indicated good correlation with reference methods (MBA, LC-FD). This is the first LFIA which has been shown, through rigorous validation, to have the ability to detect most major PSTs in a reliable manner and will be a huge benefit to both industry and regulators, who need to perform rapid and reliable testing to ensure shellfish are safe to eat.


Jawaid W.,Neogen Europe Ltd | Jawaid W.,Queen's University of Belfast | Campbell K.,Queen's University of Belfast | Melville K.,Neogen Europe Ltd | And 3 more authors.
Analytical Chemistry | Year: 2015

A single-step lateral flow immunoassay (LFIA) was developed and validated for the rapid screening of paralytic shellfish toxins (PSTs) from a variety of shellfish species, at concentrations relevant to regulatory limits of 800 μg STX-diHCl equivalents/kg shellfish meat. A simple aqueous extraction protocol was performed within several minutes from sample homogenate. The qualitative result was generated after a 5 min run time using a portable reader which removed subjectivity from data interpretation. The test was designed to generate noncompliant results with samples containing approximately 800 μg of STX-diHCl/kg. The cross-reactivities in relation to STX, expressed as mean ± SD, were as follows: NEO: 128.9% ± 29%; GTX1&4: 5.7% ± 1.5%; GTX2&3: 23.4% ± 10.4%; dcSTX: 55.6% ± 10.9%; dcNEO: 28.0% ± 8.9%; dcGTX2&3: 8.3% ± 2.7%; C1&C2: 3.1% ± 1.2%; GTX5: 23.3% ± 14.4% (n = 5 LFIA lots). There were no indications of matrix effects from the different samples evaluated (mussels, scallops, oysters, clams, cockles) nor interference from other shellfish toxins (domoic acid, okadaic acid group). Naturally contaminated sample evaluations showed no false negative results were generated from a variety of different samples and profiles (n = 23), in comparison to reference methods (MBA method 959.08, LC-FD method 2005.06). External laboratory evaluations of naturally contaminated samples (n = 39) indicated good correlation with reference methods (MBA, LC-FD). This is the first LFIA which has been shown, through rigorous validation, to have the ability to detect most major PSTs in a reliable manner and will be a huge benefit to both industry and regulators, who need to perform rapid and reliable testing to ensure shellfish are safe to eat. © 2015 American Chemical Society.


Jawaid W.,Neogen Europe Ltd | Jawaid W.,Queen's University of Belfast | Meneely J.,Queen's University of Belfast | Campbell K.,Queen's University of Belfast | And 5 more authors.
Talanta | Year: 2013

A lateral flow immunoassay (LFIA) has been developed and fully validated to detect the primary amnesic shellfish poisoning (ASP) toxin, domoic acid (DA). The performance characteristics of two versions of the test were investigated using spiked and naturally contaminated shellfish (mussels, scallops, oysters, clams, and cockles). The tests provide a qualitative result, to indicate the absence or presence of DA in extracts of shellfish tissues, at concentrations that are relevant to regulatory limits. The new rapid assay (LFIA version 2) was designed to overcome the performance limitations identified in the first version of the assay. The improved test uses an electronic reader to remove the subjective nature of the generated results, and the positive cut-off for screening of DA in shellfish was increased from 10 ppm (version 1) to 17.5 ppm (version 2). A simple extraction and test procedure was employed, which required minimal equipment and materials; results were available 15 min after sample preparation. Stability of the aqueous extracts at room temperature (22 C) at four time points (up to 245 min after extraction) and across a range of DA concentrations was 100.3±1.3% and 98.8±2.4% for pre- and post-buffered extracts, respectively. The assay can be used both within laboratory settings and in remote locations. The accuracy of the new assay, to indicate negative results at or below 10 ppm DA, and positive results at or above 17.5 ppm, was 99.5% (n=216 tests). Validation data were obtained from a 2-day, randomised, blind study consisting of multiple LFIA lots (n=3), readers (n=3) and operators (n=3), carrying out multiple extractions of mussel tissue (n=3) at each concentration (0, 10, 17.5, and 20 ppm). No matrix effects were observed on the performance of the assay with different species (mussels, scallops, oysters, clams, and cockles). There was no impact on accuracy or interference from other phycotoxins, glutamic acid or glutamine with various strip incubations (8, 10, and 12 min). The accuracy of the assay, using naturally contaminated samples to indicate negative results at or below 12.5 ppm and positive results at or above 17.5 ppm, was 100%. Variability between three LFIA lots across a range of DA concentrations, expressed as coefficient of variation (% CV), was 1.1±0.4% (n=2 days) based on quantitative readings from the electronic reader. During an 8 week stability study, accuracy of the method with test strips stored at various temperatures (6, 22, 37 and 50 C) was 100%. Validation for both versions included comparisons with results obtained using reference LC-UV methods. © 2013 Elsevier B.V.


Jawaid W.,Neogen Europe Ltd | Jawaid W.,Queen's University of Belfast | Meneely J.P.,Queen's University of Belfast | Campbell K.,Queen's University of Belfast | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

A single-step lateral flow immunoassay was developed and validated to detect okadaic acid (OA) and dinophysis toxins (DTXs), which cause diarrhetic shellfish poisoning. The performance characteristics of the test were investigated, in comparison to reference methods (liquid chromatography tandem mass spectrometry and/or bioassay), using both spiked and naturally contaminated shellfish. A portable reader was used to generate a qualitative result, indicating the absence or presence of OA-group toxins, at concentrations relevant to the maximum permitted level (MPL). Sample homogenates could be screened in 20 min (including extraction and assay time) for the presence of free toxins (OA, DTX1, DTX2). DTX3 detection could be included with the addition of a hydrolysis procedure. No matrix effects were observed from the species evaluated (mussels, scallops, oysters, and clams). Results from naturally contaminated samples (n = 72) indicated no false compliant results and no false noncompliant results at <50% MPL. Thus, the development of a new low-cost but highly effective tool for monitoring a range of important phycotoxins has been demonstrated. © 2015 American Chemical Society.


PubMed | Queen's University of Belfast, Neogen Corporation and Neogen Europe Ltd
Type: Evaluation Studies | Journal: Journal of agricultural and food chemistry | Year: 2015

A single-step lateral flow immunoassay was developed and validated to detect okadaic acid (OA) and dinophysis toxins (DTXs), which cause diarrhetic shellfish poisoning. The performance characteristics of the test were investigated, in comparison to reference methods (liquid chromatography tandem mass spectrometry and/or bioassay), using both spiked and naturally contaminated shellfish. A portable reader was used to generate a qualitative result, indicating the absence or presence of OA-group toxins, at concentrations relevant to the maximum permitted level (MPL). Sample homogenates could be screened in 20 min (including extraction and assay time) for the presence of free toxins (OA, DTX1, DTX2). DTX3 detection could be included with the addition of a hydrolysis procedure. No matrix effects were observed from the species evaluated (mussels, scallops, oysters, and clams). Results from naturally contaminated samples (n = 72) indicated no false compliant results and no false noncompliant results at <50% MPL. Thus, the development of a new low-cost but highly effective tool for monitoring a range of important phycotoxins has been demonstrated.

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