Nehru Arts and Science College
Nehru Arts and Science College
Chitra T.,Nehru Arts and Science College |
Jayashree S.,Nehru Arts and Science College |
Rathinamala J.,Nehru Arts and Science College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014
Objective: In the present study, the aqueous extract of Vetiveria zizanioides root was tested for cytotoxic effect against human cancer cell line (MCF-7 human breast cancer cell line) Methods: The aqueous extract of the root ofVetiveria zizanioideswere evaluated through MTT (3-(4,5-dimethyl thiazolyl-2 - yl)-2, 5-diphenyl tetrazolium bromide) and assessment of cell morphology by usingAcridine Orange & Ethidium Bromide staining. Results:The aqueous extract of root exhibited cytotoxicity towards the cancer cell line. IC50 concentration of 5μg/ml to 50μg/ml against human breast adenocarcinoma MCF-7 cell line was recorded. The maximum inhibition of concentration showed in 31μg/ml to 37μg/ml. Conclusion: The results indicated us the feasible anticancer nature of root aqueous crude extract.
Meenatchisundaram S.,Nehru Arts and Science College |
Michael A.,PSG College of Technology
International Journal of PharmTech Research | Year: 2010
Aqueous extracts of Mucuna pruriens seeds were tested for their activity on various pharmacological effects like lethality, phospholipase activity, edema forming activity, fibrinolytic activity and haemorrhagic activity of of cobra and krait venoms. The aqueous extract displayed a significant inhibitory effect on the lethality, phospholipase activity, edema forming activity, fibrinolytic activity and haemorrhagic activity. About 0.16 mg and 0.19 mg of Mucuna pruriens seed extracts were able to completely neutralize the lethal activity of 2LD50 of cobra and krait venom respectively. The present finding suggests that aqueous extracts of Mucuna pruriens seeds possess compounds, which inhibit the activity of cobra and krait venoms.
Balasubramanian M.,Kongu Arts and Science College |
Nirmala P.,Nehru Arts and Science College
Journal of Chemical and Pharmaceutical Research | Year: 2014
This is the first attempt reported on the antioxidant activities of Heterodermia boryi, Sticta weigelii and Dermatocarpon vellereum.The levels of super oxide dismutase were evaluated in Heterodermia boryi, Sticta weigelii and Dermatocarpon vellereum. Among which SOD levels were found to be higher in Heterodermia boryi (4460 U/g). Catalase was found to be higher in Dermatocarpon vellereum (113.01 U/g). Peroxidase activity was higher in Dermatocarpon vellereum (829.12 U/g). GST level was higher in Sticta weigelii (454.87 U/g). Polyphenol oxidase was estimated in the form of catechol and laccase. COX and LAC levels were higher in Dermatocarpon vellereum (9.973 and 9.33 U/g) and total polyphenol oxidase was found to be higher Dermatocarpon vellereum (19.23 U/g) Ascorbic acid level was higher in Sticta weigelii (24.29 mg/g) Tocopherol were reflected higher in Sticta weigelii (1474 μg/g). Reduced glutathione was found to be higher in Heterodermia boryi (11.63 nano moles/g). ABTS radicals were highly scavenged by Sticta weigelii (36.91%) followed by Dermatocarpon vellereum (35.02%) and Heterodermia boryi (31.81%).DPPH radicals were highly scavenged by Heterodermia boryi (90.66%). The inhibitory effect on lipid peroxidation by the lichens was estimated and was found that Dermatocarpon vellereum (92.26%). Comparatively Heterodermia boryi and Sticta weigelii possessed lesser LPO (94.17% and 85.51% respectively). Inhibition of oxidant induced DNA damage in herring sperm was evaluated, Sticta weigelii greatly inhibited (92.24%). © 2014, Journal of Chemical and Pharmaceutical Research. All rights reserved.
Susmitha S.,Nehru Arts and Science College |
Vidyamol K.K.,Nehru Arts and Science College |
Ranganayaki P.,Nehru Arts and Science College |
Vijayaragavan R.,Nehru Arts and Science College
Global Journal of Pharmacology | Year: 2013
Aqueous extracts of Azadirachta indica (Neem) was subjected to in vitro antibacterial assay against human pathogenic Escherichia coli and Salmonella sp by cup diffusion method. The plant leaves were effective against all the tested organisms. Minimum Bactericidal Concentration (MBC) value of 5mg/l was obtained against Escherichia coli and Salmonella sp were found to be resistant with all the solvent extracts except water. A qualitative phytochemical analysis was performed for the detection of secondary plant metabolites [viz., alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins] and reducing sugars. Thin layer chromatography (TLC) was also performed by using different solvent system for the analysis of lipid, alkaloids, flavonoids present in plant extract. The active components separated through TLC were subjected to antimicrobial activity against the pathogens. The present study will be successful in identifying candidate plant with different antimicrobial activity which could be further exploited for isolation and characterization of the novel phytochemicals in the treatment of infectious diseases especially in light of the emergence to produce more effective antimicrobial agents. © IDOSI Publications, 2013.
Mani R.,Salem College |
Subiramaniyam N.P.,Nehru Arts and Science College
Journal of Materials Science: Materials in Electronics | Year: 2017
A facile, chemical precipitation method has afforded highly crystalline iron (Fe) doped SnO2 nanoparticles with efficient photocatalytic degradation of phenol and benzoic acid under ultraviolet light irradiation. Powder X-ray diffraction results show that the both pure and Fe doped SnO2 samples are in tetragonal rutile type SnO2 phase and the results are well matched with the standard data (card no. 41-1445). Transmission electron microscope reveals that the morphology of the samples was spherical in shape and the average particle sizes were around 24–42 nm, which is in good agreement with the XRD results. UV–VIS transmission spectroscopy studies show that the band gap energy of pure SnO2 is 3.63, and 3.53, 3.34 eV for Fe-doped (3 & 5 wt%) SnO2 nanoparticles respectively. Energy dispersive spectra (EDS) spectra confirm the presence of Fe in the most active Fe-modified SnO2 sample. Fe-doped SnO2 sample exhibited enhanced activity for both phenol and benzoic acid under ultraviolet irradiation, which could be due to the Eg decreased by Fe-doping, high specific surface area and porous structure. The samples were further characterized by Fourier transform infrared (FTIR) and photoluminescence spectra analysis. The photocatalytic mechanism of Fe doped SnO2 also discussed. © 2017 Springer Science+Business Media New York
Arumugam A.,Nehru Arts and Science College |
Dhailappan A.,Mills College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012
The seeds of Nelumbo nucifera were collected from different localities of Coimbatore District. Extraction of oil from plant seed was performed using Soxhlet's procedure. Based on the physicochemical screening the lotus seed was found to be an excellent source of crude oil. The crude fat content of the ground seeds of N. nucifera was determined by GC-MS and identified by spectral library comparison and by the mass spectral fragmentation pattern. HPTLC analysis showed the presence of flavonoids. The hexane extract of the N. nucifera seed oil showed 66% inhibition of the radical DPPH. Whereas the standard ascorbic acid showed IC50 value of 15 ± 1.0 μg/ml. ABTS activity of N. nucifera seed oil (IC50 = 124 ± 1.0 μg/ml) was observed as the strongest activity. The antidiarrhoeal activity of N.nucifera seed oil was evaluated and it strongly inhibited the strains namely Shigella sp., Salmonella sp., Klebsiella sp., Escherichia Coli., Pseudomonas sp., and Staphylococcus aureus by disk diffusion method. The inhibitory activity of N.nucifera seed oil was pronounced against the dermatophytes like Malassezia furfur, Trichophyton rubrum and Trichophyton mentagrophytes with the extract concentration of 25μg/mL. This study systematically supports the usage of seed as a medicine for superficial bacterial and fungal infections, alternative source of nutrition and as well as renewable resources.
Anitha A.,Nehru Arts and Science College
International Journal of Pharma and Bio Sciences | Year: 2012
Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. A feather-degrading bacterium with high keratinase activity was isolated and identified as Bacillus megaterium (A1). The selected organism showed keratinase activity of 72.875 IU/mg and the protein content of 4 mg/ml. Maximum enzyme production was observed on 96 hr. The intense feather degrading was achieved in 35°C and initial pH adjusted to 7.5. Among the extra nitrogen or carbon sources used, significant improvement in yield of keratinase was obtained when grown in medium containing 2% feather meal. The protein profile was analyzed in SDS-PAGE showed multiple bands. Zymography analysis showed a single band with molecular weight of 46 KDa which corresponds to keratinase activity. Immobilized cells of B. megaterium (A1) had highest durability to degrade feather keratin. Fourier Transform Infrared Spectroscopy (FTIR) showed that the change in the functional group was catalyzed by the unique enzymes of B. megaterium (A1). From this study, Bacillus megaterium isolated from feather dumped soil can be used as a potential candidate for degradation of feather and for can be used as additives in poultry field.
Rabeeth M.,Chikkanna Government Arts College |
Anitha A.,Nehru Arts and Science College |
Srikanth G.,Amrita University
Pakistan Journal of Biological Sciences | Year: 2011
Streptomyces griseus (MTCC 9723) is a chitinolytic bacterium isolated from prawn cultivated pond soil of Peddapuram Village; East Godavari District was studied in detailed. Chitinase (EC 184.108.40.206) was extracted from the culture filtrate of Streptomyces griseus and purified by ammonium sulfate precipitation, DEAE-cellulose ionexchange chromatography, Sephadex G-100 and Sephadex G-200 gel filtration chromatography. The molecular mass of the purified chitinase was estimated to be 34, 32 kDa by SDS gel electrophoresis and confirmed by activity staining with Calcofluor White M2R. Chitinase was optimally active at pH of 6.0 and at 40°C. The enzyme was stable from pH 5-9 and up to 20-50°C. The chitinase exhibited Km and Vmax values of 400 mg and 180 IU mL -1 for colloidal chitin. Among the metals and inhibitors that were tested, the Hg +, Hg 2+ and P-chloromercuribenzoic acid completely inhibited the chitinase activity at 1 mM concentration. The purified chitinase showed high activity on colloidal chitin, chitodiose, and chitooligosaccharide. An in vitro assay proved that the crude chitinase, actively growing cells of S. griseus having antifungal activity against all studied fungal pathogen. This result implies that characteristics of S. griseus producing endochitinase made them suitable for biotechnological purpose such as for degradation of chitin containing waste and it might be a promising biocontrol agent for plant pathogens. © 2011 Asian Network for Scientific Information.
Priya C.A.,PSGR Krishnammal College For Women |
Balasaravanan T.,Nehru Arts and Science College |
Thanamani A.S.,Nallamuthu Gounder Mahalingam College
International Conference on Pattern Recognition, Informatics and Medical Engineering, PRIME 2012 | Year: 2012
Recognition of plants has become an active area of research as most of the plant species are at the risk of extinction. This paper uses an efficient machine learning approach for the classification purpose. This proposed approach consists of three phases such as preprocessing, feature extraction and classification. The preprocessing phase involves a typical image processing steps such as transforming to gray scale and boundary enhancement. The feature extraction phase derives the common DMF from five fundamental features. The main contribution of this approach is the Support Vector Machine (SVM) classification for efficient leaf recognition. 12 leaf features which are extracted and orthogonalized into 5 principal variables are given as input vector to the SVM. Classifier tested with flavia dataset and a real dataset and compared with k-NN approach, the proposed approach produces very high accuracy and takes very less execution time. © 2012 IEEE.
Pushpaja P.V.,Nehru Arts and Science College
International Journal of Agricultural and Statistical Sciences | Year: 2015
Here, we present a new parametric method of testing skewness using order statistics. By deriving the first four central moments of the test statistic, the distribution of it is fitted as a member in the Generalized Lambda Distribution (GLD) family. The method is based on computer programmes in Maple language. Tables representing the percentile points of the sampling distribution of the test statistic under different distributions and for different sample sizes are provided. The power of the test is also obtained. This method is too general as there is no specific distributional assumptions.