Meenatchisundaram S.,Nehru Arts and Science College |
Michael A.,PSG College of Technology
Iranian Journal of Biotechnology | Year: 2010
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were compared in terms of total protein content, immunospecific anti E. carinatus immunoglobulin Y (IgY) activity and in vitro and in vivo neutralizing capacity of IgY against the E. carinatus venom. Total IgY concentrations varied from 1.6 to 7.0 mg per ml of egg yolk. In neutralization studies, IgY purified by PEG and ammonium sulphate precipitation (PEG-AS) showed better results when compared to other purification methods. Approximately 1.25 mg of IgY (PEG-AS) was able to neutralize 2Lethal Dose50 of the E. carinatus venom. Purification of IgY by PEG and ammonium sulphate yielded very pure IgY at high quantities (93% ± 5% of total egg yolk protein), which was also capable of neutralizing toxic and lethal components of the E. carinatus venom.
Anitha A.,Nehru Arts and Science College
International Journal of Pharma and Bio Sciences | Year: 2012
Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable byproduct. A feather-degrading bacterium with high keratinase activity was isolated and identified as Bacillus megaterium (A1). The selected organism showed keratinase activity of 72.875 IU/mg and the protein content of 4 mg/ml. Maximum enzyme production was observed on 96 hr. The intense feather degrading was achieved in 35°C and initial pH adjusted to 7.5. Among the extra nitrogen or carbon sources used, significant improvement in yield of keratinase was obtained when grown in medium containing 2% feather meal. The protein profile was analyzed in SDS-PAGE showed multiple bands. Zymography analysis showed a single band with molecular weight of 46 KDa which corresponds to keratinase activity. Immobilized cells of B. megaterium (A1) had highest durability to degrade feather keratin. Fourier Transform Infrared Spectroscopy (FTIR) showed that the change in the functional group was catalyzed by the unique enzymes of B. megaterium (A1). From this study, Bacillus megaterium isolated from feather dumped soil can be used as a potential candidate for degradation of feather and for can be used as additives in poultry field.
Jayashree S.,Nehru Arts and Science College |
Rathinamala J.,Nehru Arts and Science College |
Lakshmanaperumalsamy P.,Karpagam University
Journal of Environmental Science and Technology | Year: 2011
A pot culture study was conducted using textile wastewater contaminated soil which was amended with Vermicompost (VC) in various proportions for a period of two months. The plant used for the study was Chrysopogon zizanioides (Vetiver) to investigate the accumulation of heavy metals in their roots. Physico-chemical parameters like pH, EC, TKN, P, K, TOC and metals like Pb, Cd and Cu and microbial population of the textile wastewater contaminated soil were analyzed initially (0 day) and finally (60th day). The growth parameters of vetiver like root length, shoot length, fresh weight and dry weight were also recorded initially and finally. Based on the data C. zizanioides (Vetiver) tolerated and accumulated the greatest amount of heavy metals. C. zizanioides could uptake more lead than the other metals. The effect of vermicompost on the growth of C. zizanioides showed that the biomass was increased when the vermicompost concentration was increased. The microbial population like bacteria, actinomycetes and fungi was more in the rhizosphere soil than in non-rhizosphere soil. © 2011 Asian Network for Scientific Information.
Maheshu V.,Nehru Arts and Science College |
Priyadarsini D.T.,Karpagam University |
Sasikumar J.M.,Karpagam University
Journal of Food Science and Technology | Year: 2012
Caralluma adscendens (Roxb.) Haw var. fimbriata (wall.) Grav. & Mayur. is a traditional food consumed as vegetable or pickle in arid regions of India and eaten during famines. In Indian traditional medicine, the plant is used to treat diabetes, inflammation and etc. The aim of this study was to evaluate the antioxidant properties (DPPH, TEAC, TAA, FRAP, OH˙ and NO˙ radical scavenging activities) of the different extracts from aerial parts. The levels of total phenolics and flavonoids of the extracts were also determined. The extracts were found to have different levels of antioxidant properties in the test models used. Methanol and water extracts had good total phenolic and flavonoid contents showed potent antioxidant and free radical scavenging activities. The antioxidant activity was correlated well with the amount of total phenolics present in the extracts. The extracts and its components may be used as an additive in food preparations and nutraceuticals. © 2012, Association of Food Scientists & Technologists (India).
Rabeeth M.,Chikkanna Government Arts College |
Anitha A.,Nehru Arts and Science College |
Srikanth G.,Amrita University
Pakistan Journal of Biological Sciences | Year: 2011
Streptomyces griseus (MTCC 9723) is a chitinolytic bacterium isolated from prawn cultivated pond soil of Peddapuram Village; East Godavari District was studied in detailed. Chitinase (EC 22.214.171.124) was extracted from the culture filtrate of Streptomyces griseus and purified by ammonium sulfate precipitation, DEAE-cellulose ionexchange chromatography, Sephadex G-100 and Sephadex G-200 gel filtration chromatography. The molecular mass of the purified chitinase was estimated to be 34, 32 kDa by SDS gel electrophoresis and confirmed by activity staining with Calcofluor White M2R. Chitinase was optimally active at pH of 6.0 and at 40°C. The enzyme was stable from pH 5-9 and up to 20-50°C. The chitinase exhibited Km and Vmax values of 400 mg and 180 IU mL -1 for colloidal chitin. Among the metals and inhibitors that were tested, the Hg +, Hg 2+ and P-chloromercuribenzoic acid completely inhibited the chitinase activity at 1 mM concentration. The purified chitinase showed high activity on colloidal chitin, chitodiose, and chitooligosaccharide. An in vitro assay proved that the crude chitinase, actively growing cells of S. griseus having antifungal activity against all studied fungal pathogen. This result implies that characteristics of S. griseus producing endochitinase made them suitable for biotechnological purpose such as for degradation of chitin containing waste and it might be a promising biocontrol agent for plant pathogens. © 2011 Asian Network for Scientific Information.
Gobi M.,Chikkanna Government Arts College |
Kannan D.,Nehru Arts and Science College
ARPN Journal of Engineering and Applied Sciences | Year: 2015
The multimedia content protection has become a boon for an IT industry nowadays. This scheme is based on layered encryption/decryption involving biometric authentication. Utilization of fingerprints as keys in encryption/decryption procedures eliminates the feasibility of illegal key sharing, which hampers the content protection schemes based solely on traditional keys. The computation times required for the necessary encryption and decryption processes are provided for AES symmetric-key system and HECC asymmetric-key system. These times show the applicability of the method. Utilization of widely available encryption/decryption systems (e.g., AES and HECC) increases the applicability even further. Custom hardware chips will reduce these times in future applications. © 2006-2015 Asian Research Publishing Network (ARPN).
Jenneth A.,Nehru Arts and Science College |
Thangavel K.,Periyar University
International Journal of Applied Engineering Research | Year: 2016
Data Exploration arise naturally in many domains, and have regularly presented a great challenge for traditional data mining techniques, both in terms of effectiveness and efficiency. Clustering becomes difficult due to the increasing sparsity of such data, as well as the increasing difficulty in distinguishing distances between data points. In this paper, we propose shared neighbour Clustering approach based on the affinity propagation in the data points on high dimensional data. Proposed Technique not only takes advantage of the affinity propagation algorithm in terms similarity estimation through multi distance function and also possesses to prune the noisy attributes which characterises through Hubness property. It is good measure of point centrality within a high-dimensional data cluster and that major hubs can be used effectively as cluster prototypes. The evaluation shows that our algorithms frequently offer improvements in cluster quality and homogeneity in the multiple setting. © Research India Publications.
Priya C.A.,PSGR Krishnammal College For Women |
Balasaravanan T.,Nehru Arts and Science College |
Thanamani A.S.,Nallamuthu Gounder Mahalingam College
International Conference on Pattern Recognition, Informatics and Medical Engineering, PRIME 2012 | Year: 2012
Recognition of plants has become an active area of research as most of the plant species are at the risk of extinction. This paper uses an efficient machine learning approach for the classification purpose. This proposed approach consists of three phases such as preprocessing, feature extraction and classification. The preprocessing phase involves a typical image processing steps such as transforming to gray scale and boundary enhancement. The feature extraction phase derives the common DMF from five fundamental features. The main contribution of this approach is the Support Vector Machine (SVM) classification for efficient leaf recognition. 12 leaf features which are extracted and orthogonalized into 5 principal variables are given as input vector to the SVM. Classifier tested with flavia dataset and a real dataset and compared with k-NN approach, the proposed approach produces very high accuracy and takes very less execution time. © 2012 IEEE.
Pushpaja P.V.,Nehru Arts and Science College
International Journal of Agricultural and Statistical Sciences | Year: 2015
Here, we present a new parametric method of testing skewness using order statistics. By deriving the first four central moments of the test statistic, the distribution of it is fitted as a member in the Generalized Lambda Distribution (GLD) family. The method is based on computer programmes in Maple language. Tables representing the percentile points of the sampling distribution of the test statistic under different distributions and for different sample sizes are provided. The power of the test is also obtained. This method is too general as there is no specific distributional assumptions.
Hemamalini G.,Nehru Arts and Science College |
Nirmala P.,Nehru Arts and Science College
International Journal of Pharma and Bio Sciences | Year: 2015
In the present study the immune response level and various immunological properties in experimental mice was assessed after administering various extracts of A. nilotica and immunosuppressive drugs. The ethanol extract of A. nilotica showed highest antibody titre (7 log2 2) followed by hexane and chloroform extract (6 log2 2) and butanol and water extract (5.5 log2 2). The increment in 'B' lymphocyte number was much pronounced in mice by the administration of A. nilotica in combination with immunoenhansive drug than the lone effect of immunoenhansive drug. B cell decrement was pronounced in mice treated with immunosuppressive drug while a moderate decrement was noticed due to A. nilotica in combination with immunosuppressive drug. The increment in blood cell counts was much pronounced in mice by the administration of A. nilotica in combination with immunoenhansive drug than the lone effect of immunoenhansive drug. The increment in 'T' cell count may be due to the impact of plant drug on the synthesis, proliferation and activation of 'T' cells in treated animals.