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Provided is a settlement and remittance-processing method, wherein processing that involves transfer of virtual money, such as settlement or remittance, is achieved with a single system. A virtual money management apparatus includes an account management storage section which stores a value of the virtual money owned by the users and company; an account management function section which includes virtual money accounts for each of the users and company and associates the virtual money accounts with the virtual money stored in the account management storage section to manage virtual money using the virtual money accounts; and a settlement/remittance management function section which receives designation of the user or company which is to be a transfer destination of the virtual money, specifies a virtual money account associated with the designated user or company by using the account management function section, and transfers the virtual money to the specified virtual money account.


The need for input and transmission of information necessary for settlement each time accounts are settled is eliminated. Necessary information to settle accounts by using a payment system is received from a terminal used by a user and stored. The received information and the payment system using the information are tied and managed for each user. When settlement processing of a certain user is requested from a business operator site, the payment systems that can be used by the certain user are identified by using an account management function unit and the settlement processing by the selected payment system is performed by using the necessary information tied to the payment system selected by the certain user from among the identified payment systems.


The object of the present invention is to provide a method for identifying a nucleotide sequence necessary for expressing affinity for a target substance with respect to a nucleotide sequence of a nucleic acid molecule such as an aptamer having such affinity for the target substance, based on similarity between nucleotide sequences and an evaluated value of the affinity of the nucleotide sequence, and a method for predicting a secondary structure of the nucleic acid molecule including the identified nucleotide sequence. The method of present invention includes the steps of extracting a single-stranded region by excluding based capable of forming a stem structure from the nucleotide sequence of the nucleic acid molecule; and searching a motif sequence from the single-stranded region, based on an evaluated value of the affinity.


One settlement is made with multiple settlement units. A user-selected settlement unit, at least one settlement price allocated to each settlement unit and a usage ratio allocated to each settlement unit, and a total price to be settled are received from a site. Credit processing is performed for each price obtained by allocating the total price to the settlement unit using the usage ratio or for each settlement price allocated to each settlement unit. Sales determination processing is performed for each price obtained by allocating the total price to the settlement unit using the usage ratio or for each settlement price allocated to each settlement unit. A message indicating settlement completion is transmitted to the site. In the first and second settlements, when all credit processing and sales determination processing can be performed within the multi-settlement apparatus, the processing is so performed, otherwise, an external unit is requested.


The present invention is to provide a new detection method that is superior in detection sensitivity and allows a simple operation. A nucleic acid construct that includes a cloning region, an encoding nucleic acid of a peptide tag, an encoding nucleic acid of an aptamer that is bindable to the peptide tag is used. By inserting the encoding nucleic acid of arbitrary peptide into the cloning region of the nucleic acid construct, the nucleic acid construct is expressed in vivo. Thereby, a complex of a fusion transcript having the base sequence that includes the encoding nucleic acid of arbitrary peptide, the encoding nucleic acid of a peptide tag, and the encoding nucleic acid of the aptamer and a fusion translation that includes the arbitrary peptide and the peptide tag is formed. By bringing the complex into contact with a target and recovering the complex that is bound to the target, the peptide that is bindable to a target and its encoding nucleic acid can be identified from the transcript of the encoding nucleic acid of arbitrary peptide in the complex.

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