Khandare J.J.,NCE Polymer Chemistry Group Piramal Life science Ltd. |
Jalota-Badhwar A.,Oncology Piramal Life science Ltd. |
Satavalekar S.D.,NCE Polymer Chemistry Group Piramal Life science Ltd. |
Bhansali S.G.,NCE Polymer Chemistry Group Piramal Life science Ltd. |
And 3 more authors.
We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe 3O 4), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe 3O 4-PEG-FITC-CNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe 3O 4 nanoparticles were well anchored onto the surfaces of the CNT. In vitro time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe 3O 4-PEG-FITC-CNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with in vitro non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe 3O 4-PEG-FITC-CNT nanosystem an ideal candidate for bioimaging, both in vitro and in vivo. © 2012 The Royal Society of Chemistry. Source