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New Delhi, India

Rana J.C.,National Bureau of Plant Genetic Resources Regional Station | Dutta M.,NBPGR | Rathi R.S.,NBPGR Regional Station
Indian Journal of Genetics and Plant Breeding | Year: 2012

The Indian Himalayan region houses wide array of plant genetic resources due to its diverse climatic conditions. India's recognition as a 'mega-biodiversity' country derives partly from the Himalayas wherein out of 6000 endemic plant species, 2532 species occur. Major genera for which rich diversity exists are-Oryza, Avena, Amaranthus, Chenopodium, Fagopyrum, Allium, Hordeum, Linum, Saccarum, Citrus, Musa, Pyrus, Prunus, Rubus, Fragaria, Sorbus, Rosa,Lilium, Vicia, Lepidium, Lathyrus, Dioscorea, Orchids, Cucumis, Solanum, and Trichosanthes, Bamboos and Canes. These plant species and varieties have contributed significantly to food and environment security of the hill people. It also provides stability to the food production systems by minimising crop losses due to diseases, insect pests and weather odds. Besides, there are several wild relatives possess genes for various purposes and has lot of breeding value. Despite that, the most distressing situation is that many of these valuable genetic resources are eroding at very high rate. The crops, folk varieties and landraces of local importance adapted to specific micro-climatic niches are being replaced by a few high yielding varieties and cash crops. Nonetheless, appreciable amount of diversity has been collected and being conserved ex situ by different institutions particularly the NBPGR and NAGS. Nearly, 300 cropspecific and multi-crops exploration trips have been undertaken and more than 30,000 germplasm accessions of various agri-horticultural crops including some of their wild relative have been assembled from Himalayan region while around 6000 have been introduced from exotic sources. Source


Deswal R.P.S.,Regional Research Station | Panwar N.S.,NBPGR | Rawat L.,ICFRE | Bangarwa K.S.,Ch Charan Singh Haryana Agricultural University
Indian Journal of Ecology | Year: 2014

Karanj mature pods were collected from the three states viz. Haryana, Punjab and Rajasthan in two consecutive years 2009 and 2010. Intra-cluster average D2 value of cluster V was minimum (1.238) thus revealed the minimum genetic diversity and cluster IV had maximum (2.471), which indicated the existence of maximum genetic diversity. The average Inter cluster D 2 value between cluster IV and V was maximum (7.176), whereas, the minimum Inter-cluster average D2 value was in cluster I and V (2.514). The genotypes which had highest cluster means and showed higher inter-cluster divergence with several other characters would be considered as putative parents for hybridization. Hybridization among genotypes of the same cluster may not provide a good scope for obtaining useful segregates. Source


Mawlong I.,Indian Agricultural Research Institute | Ali K.,Indian Agricultural Research Institute | Kurup D.,Indian Agricultural Research Institute | Yadav S.,NBPGR | Tyagi A.,Indian Agricultural Research Institute
Journal of Plant Biochemistry and Biotechnology | Year: 2014

A genomic DNA fragment of 964 bp corresponding to AP2/ERF family transcription factor was isolated from drought tolerant Oryza sativa L. cv. 'N-22' (AP2/ERF-'N-22') and Oryza sativa L. cv. 'Teipei 309' (AP2/ERF-jap). The cDNA sequence of AP2/ERF-'N-22' (732 bp) was amplified using gene specific primers. The gene contains two exons and a single intron. The deduced protein of the AP2/ERF-'N-22' contains a potential nuclear localization signal, a possible regulation domain and an AP2 DNA binding domain of 60 amino acids. Northern and RT-PCR analysis showed that transcript of AP2/ERF-'N-22' accumulates in response to WDS and the Southern analysis indicated the presence of single copy of AP2/ERF-'N-22' gene in Oryza sativa genome. Phylogenetic analysis of AP2/ERF family revealed that AP2/ERF-'N-22' belongs to group Va along with SHN clade of AP2/ERF protein which activates wax biosynthesis. A higher epicuticular wax content was observed at decreased RWC and positively correlated with AP2/ERF-'N-22' expression. © 2012 Society for Plant Biochemistry and Biotechnology. Source


Kundu M.,Indian Agricultural Research Institute | Dubey A.,Indian Agricultural Research Institute | Srivastav M.,Indian Agricultural Research Institute | Malik S.,NBPGR | Singh B.,Indian Agricultural Research Institute
Turkish Journal of Biology | Year: 2014

The present work analyzed the stainability and in vitro germinability of the gamma-ray-irradiated fresh and cryopreserved pollens of 3 Citrus species: C. limon, C. limetta, and C. sinensis. Fruit set after pollinating 75 flowers of C. grandis with irradiated and nonirradiated (control) fresh and cryopreserved pollen was also assessed. Results showed that in solid and liquid culture media the highest in vitro germination values were detected in fresh (61.45% and 62.53%, respectively) as well as cryopreserved (53.17% and 55.36%, respectively) C. sinensis nonirradiated pollens. However, at a higher irradiation dose of 500 Gy in both culture media, the minimum reduction of germinability of fresh (30.85% and 28.01%) and cryopreserved pollens (33.37% and 31.06%) was found in C. limon. Contrary to in vitro germination, the minimum reduction in stainability of fresh and cryopreserved pollens, as assessed by acetocarmine (9.51% and 11.85%, respectively) and FDA tests (10.78% and 12.73%, respectively), was recorded in C. limetta. Regardless of irradiation dose, the highest fruit setting in C. grandis at 40 days after pollination was detected when it was pollinated with both fresh (38.02%) and cryopreserved (34.92%) C. limetta pollen grains. © TÜBİTAK. Source


Roy S.,National Bureau of Plant Genetic Resources NBPGR | Rathi R.S.,National Bureau of Plant Genetic Resources NBPGR | Misra A.K.,National Bureau of Plant Genetic Resources NBPGR | Bhatt B.P.,ICAR Research Complex for NEH Region | And 2 more authors.
Plant Genetic Resources: Characterisation and Utilisation | Year: 2014

Nagaland is one of the eight states in the north-eastern region of India, where a considerable diversity exists in cultivated rice. Recent exploration in this tribal-dominated state has resulted in a collection of 130 rice accessions growing under diverse agroecological conditions. The agromorphological characterization data of 124 rice landraces revealed a significant variability in plant architecture and grain morphological and quality traits. Multivariate analyses including principal component analysis (PCA) and cluster analysis were performed to assess the patterns of morphological variation. The PCA extracted 12 components, which explained 75.4% of the total variation for 38 quantitative and qualitative traits. The cluster analysis grouped 124 rice landraces into five clusters, and the number of landraces in each cluster ranged from 1 to 59. The correlations among the traits are discussed, which will be useful in considering traits for genetic improvement in grain yield and quality. The landraces have been conserved in the national genebank for further utilization. © 2013 NIAB. Source

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