Lucknow, India
Lucknow, India
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Mishra R.K.,Fish Health Management Division | Verma D.K.,Fish Health Management Division | Ravindra,Fish Health Management Division | Yadav M.K.,Fish Health Management Division | And 3 more authors.
Journal of Environmental Biology | Year: 2017

Aim: Knowledge about the structure of microbial community is essential to assess the risks of exposure of farmed fish to pathogens and studying the antibiotic resistance. The present study was carried out to assess the bacterial diversity in afreshwater ecosystem. Methodology: In the present study, microbial composition of a wetland in Lakhimpur-Kheri (N 27° 90.25′ and E 80° 79.80′), Uttar Pradesh was assesesed employing amplified rDNA restriction analysis (ARDRA), using Haelll enzyme as well as 16S rDNA gene sequencing. Furthermore, antibiotic resistance of the bacterial isolates was determined on the basis of multiple antibiotic resistance (MAR) index. Results: A total of one hundred and thirty five bacteria were isolated and the restriction analysis of amplified 16S rDNA gene of the above isolates, using Haelll enzyme, indicated that isolated bacteria belonged to 33 single member phylotypes. Molecular characterization of the isolates revealed that these phylotypes belonged to 20 genera under 6 classes viz., Gammaproteobacteria (72.59%), Betaproteobacteria (4.44%), Flavobacteria (9.63%), Bacilli (7.41%) Actinobacteria (3.70%) and Sphingobacteria (2.22%). In the present study, four species i.e. Sphingobacterium kitahiroshimense, Yersinia nurmii, Pseudomonas koreensis and Vogesella alkaliphila have been reported for the first time from a wetland. The antibiotic sensitivity test indicated that most of the isolates were sensitive to norfloxacin followed by ofloxacin, whereas, majority of these bacteria were resistant to penicillin G followed by polymyxin B. Moreover, MAR indexing revealed that amongst the 33 single member phylotypes, 17 were multiple antibiotic resistant. Interpretation: The bacterial diversity analysis of the wetland indicated bacterial population of aquatic, soil, animal and plant origin. Moreover, the study indicated that wetland can be a reservoir for multidrug resistant bacteria even when there is no application of antibiotics for aquaculture purposes. It can be assumed that the occurrence of antibiotic resistance and MAR among bacteria isolated from wetland could be due to flow of runoff from mainland. These results would help in strengthening our knowledge of bacterial ecology of the wetland ecosystem. © Triveni Enterprises, Lucknow (India).

Abdul Muneer P.M.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Gopalakrishnan A.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Musammilu K.K.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Basheer V.S.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | And 4 more authors.
Biochemical Genetics | Year: 2012

The comparative assessment of genetic diversity using allozymes, random amplified polymorphic DNA (RAPD), and microsatellite markers was conducted in endemic and endangered yellow catfish (Horabagrus brachysoma) sampled from three locations in Western Ghats river systems of India. Among the three markers, microsatellites show more polymorphism, having 100% polymorphic loci, whereas allozymes show the least (56%). In RAPD, 60.5% of fragments were polymorphic. Observed heterozygosity and F ST values were very high in microsatellites, compared with the other markers. Microsatellite and RAPD markers reported a higher degree of genetic differentiation than allozymes among the populations depicted by pairwise F ST/G ST, AMOVA, Nei's genetic distance, and UPGMA dendrogram. The three classes of markers demonstrated striking genetic differentiation between pairs of H. brachysoma populations. The data emphasize the need for fishery management, conservation, and rehabilitation of this species. © 2011 Springer Science+Business Media, LLC.

Chattopadhyay D.N.,Indian Central Institute of Freshwater Aquaculture | Mohapatra B.C.,IFAPA Centro del Llano | Adhikari S.,IFAPA Centro del Llano | Pani K.C.,IFAPA Centro del Llano | And 2 more authors.
Aquaculture International | Year: 2013

Labeo rohita (139.92 ± 0.76 mm/24.33 ± 0.45 g) was reared for 92 days in floating square cages (10 m2 area, 1.5 m height) in a pond (2 ha) at six stocking densities (5, 7.5, 10, 15, 20 and 25 fish m-2) each with 3 replicates. Fish were fed daily once in the morning with rice polish and groundnut oil cake (1:1) in dough form at 3 % of the total body weight. Survival ranged from 96 to 100 % in different stocking densities. Final average body weight, average body weight gain, mean daily body weight gain and SGR decreased (P < 0.05) with increasing stocking density. Conversely, final biomass, biomass gain and FCR increased (P < 0.05) with increasing stocking density. The highest growth rate of fish could be achieved up to 60 days at 5 fish m-2 and 92 days at other densities. The reduced growth rate at 10-25 fish m-2 for 60 days of culture indicated that stress is related to size and density of the fish, suggesting that utmost care is required to reduce the stress at high densities. Maximum production and profit was observed at the highest stocking density. Non-lethal levels of water and soil qualities at different sites (cage premises, and 20 and 200 m away from cage area) suggested that cage aquaculture could be done safely covering 0.9 % of pond area. Production of advanced fingerlings in cages was found a viable alternative to their culture in pond. © 2012 Springer Science+Business Media B.V.

Musammilu K.K.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Abdul-Muneer P.M.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Gopalakrishnan A.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | Basheer V.S.,National Bureau of Fish Genetic Resources NBFGR Cochin Unit | And 5 more authors.
Molecular Biology Reports | Year: 2014

Gonoproktopterus curmuca is an endangered red tailed barb found in Southern part of Western Ghat, India. As a part of stock-specific, propagation assisted rehabilitation and management program, polymorphic microsatellites markers were used to study the genetic diversity and population structure of this species from the three River systems of Southern Western Ghats, such as Periyar River, the Chalakkudy River, and the Chaliyar River. From selected eight polymorphic microsatellite markers, the number of alleles per locus ranged from 2 to 8, and the average number of alleles among 3 populations ranged from 5.0 to 5.75. The mean observed (Hob) and expected (Hex) heterozygosity ranged from 0.5148 to 0.5360 and from 0.5996 to 0.6067, respectively. Significant deviations from Hardy - Weinberg Equilibrium expectation were found at majority of the loci (except Gcur MFW72 and Gcur MFW19) and in all three populations in which heterozygote deficits were apparent. The analysis of molecular variance indicates that the percent of variance among populations and within populations were 6.73 and 93.27, respectively. The pairwise FST values between populations indicate that there were significant deviations in genetic differentiations for the red-tailed barb populations from these three Rivers of the Western Ghats, India. The microsatellites methods reported a low degree of gene diversity and lack of genetic heterogeneity in the population of G. curmuca, which strongly emphasize the need of fishery management, conservation and rehabilitation of G. curmuca. © Springer Science+Business Media 2014.

veer Singh K.,NBFGR | Gopalakrishnan A.,NBFGR | Lakra W.S.,NBFGR | Sobti R.C.,University of Punjab
Marine Biodiversity | Year: 2012

Genetic diversity of seahorse species was studied using 12 polymorphic microsatellite DNA loci in the yellow seahorse Hippocampus kuda and the three-spotted seahorse H. trimaculatus from samples collected along the east and west coasts of India. Cross-species microsatellite loci amplification in populations of Indian seahorses showed significant numbers of private alleles associated with site-specific populations, which could be used for population genetic studies and management of species for conservation. Significant genotype heterogeneity indicated that the samples are not from the same gene pool, and that physical barriers exist which inhibit breeding between populations. The identified microsatellite loci can be further utilized in fine-scale population structure analysis of seahorses. © 2012 Senckenberg Gesellschaft für Naturforschung and Springer.

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