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Nayoro, Japan

Nayoro City University is a public university in Nayoro, Hokkaido, Japan. The school was established as a junior women's college in 1960, and it became a four-year college in 2006. Wikipedia.

Oku T.,Kagoshima University | Ando S.,Nayoro City University | Tsai H.-C.,Kagoshima University | Yamashita Y.,Kagoshima University | And 4 more authors.
Biochimie | Year: 2012

Three enzymes, carnosine dipeptidase I (EC, CNDP1), carnosine dipeptidase II (EC, CNDP2), and Xaa-methyl-His dipeptidase (or anserinase: EC, ANSN), are known to be capable of catalyzing the hydrolysis of carnosine (β-alanyl-l-histidine), in vertebrates. Here we report the purification and identification of two unidentified carnosine-cleaving enzymes from Japanese eel (Anguilla japonica). Two different dipeptidases were successfully purified to homogeneity from the skeletal muscle; one exhibited a broad substrate specificity, while the other a narrow specificity. N-terminal amino-acid sequencing, deglycosylation analysis, and genetic analysis clearly revealed that the former is a homodimer of glycosylated subunits, encoded by ANSN, and the latter is another homodimer of glycosylated subunits, encoded by CNDP1; that is, Xaa-methyl-His dipeptidase, and carnosine dipeptidase I respectively. This is the first report on the identification of carnosine dipeptidase I from a non-mammal. Database search revealed presence of a CNDP1 ortholog only from salmonid fishes, including Atlantic salmon and rainbow trout, but not from other ray-finned fish species, such as zebrafish, fugu, and medaka whose genomes have been completely sequenced. The mRNAs of CNDP1 and ANSN are strongly expressed in the liver of Japanese eel, compared with other tissues, while that of CNDP2 is widely distributed in all tissues tested. © 2012 Elsevier Masson SAS. All rights reserved. Source

Hirohashi Y.,Nayoro City University | Lee S.-J.,Yasu Jikei kai social welfare corporation
Communications in Computer and Information Science | Year: 2014

We measured the finger plethysmograms of care workers working in a social welfare facility for the elderly three times a day, and gained the chaotic information was analyzed using a non linear analysis method. As a result, the differences were found in the value of the Lyapunov exponent as vital information reflecting the level of mental revitalization, the value of autonomic balance, the degree to which an individual is unable to shake off something that happened and worrying about the gap between personal emotions and what is expected of an individual as a professional in the case of Emotional Labor. The 4 patterns became clear, like the Tense Group, the Fatigued Group, the Stable Group, and the Easygoing Group. It can be said that the improvement of emotional control skills is important for a care for the elderly. © Springer-Verlag Berlin Heidelberg 2014. Source

Takubo K.,Tokyo Metropolitan University | Fujita M.,Nayoro City University | Izumiyama N.,Tokyo Metropolitan University | Nakamura K.-I.,Tokyo Metropolitan University | And 8 more authors.
Journal of Pathology | Year: 2010

Chromosomal and genomic instability due to telomere dysfunction is known to play an important role in carcinogenesis. To study telomere dysfunction in the surrounding background epithelium of squamous cell carcinoma in situ (CIS) of the oesophagus, we measured telomere lengths of basal and parabasal cells of epithelia with and without CIS using quantitative fluorescence in situ hybridization (Q-FISH) and our original software, Tissue Telo. Additionally, we assessed histological inflammation and the anaphase bridge index. In non-cancerous epithelium, telomeres in basal cells were significantly longer than those in parabasal cells, whereas CIS showed a homogeneous telomere pattern in the basal and parabasal cells. Telomeres in basal and parabasal cells were significantly shorter in the background with CIS than in epithelium from age-matched normal controls. Significant negative correlation was observed between the normalized telomere : centromere ratio (reflected telomere length) and the anaphase bridge index in non-cancerous epithelia from both normal controls and the CIS background with no histological inflammation. These findings indicate that tissue stem cells may be located among basal cells, and that telomere length distribution in component cell types differs between CIS and non-cancerous epithelium. We have demonstrated conclusively that oesophageal CIS arises from epithelium with short telomeres and chromosomal instability in the absence of histological inflammation. Copyright © 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. Source

Yoshii S.,Keiyukai Sapporo Hospital | Yoshii S.,Nippon Telegraph and Telephone | Nojima M.,Sapporo Medical University | Nojima M.,Tokyo Medical University | And 9 more authors.
Clinical Gastroenterology and Hepatology | Year: 2014

Background & Aims: More information is needed on the long-term outcomes of patients who undergo endoscopic resection of colorectal tumors. We evaluated recurrence of colorectal cancer (CRC) after endoscopic resection or a combination of endoscopic research and surgery for T1 colorectal tumors. Methods: We conducted a retrospective study of 389 patients with T1 CRC treated by endoscopic resection from January 1989 to December 2008 in Sapporo, Japan. We compared outcomes between patients who underwent subsequent surgery (ER+ SURG, n= 205) and those who did not (ER only, n= 184) and statistically adjusted baseline differences between the groups according to the propensity scores. Results: There was almost no risk of cancer recurrence among patients without indications for surgery recommended by the Japanese Society for Cancer of the Colon and Rectum (these indications include tumors with vertical margins, deep submucosal invasion, lymphatic or venous invasion, poor differentiation, or high-grade budding). Among patients with indications for surgery, the cumulative risks of recurrence (CRRs) were 3.7% in the ER+ SURG group and 20.1% in the ER only group (P= .001). However, the patients with only deep submucosal invasion had a low CRR, even without surgery (2.3% in the ER+ SURG group and 3.4% in the ER only groups, P=.867). In contrast, patients with indications for surgery other than deep submucosal invasion (high-risk patients) had much better outcomes when they also underwent surgery (CRRs: 5.8% in the ER+ SURG group vs 58.0% in the ER only group, P < .001). Conclusions: On the basis of a retrospective study of patients who underwent endoscopic resection for T1 CRC, those with tumors with only submucosal invasion are at low risk for cancer recurrence. However, patients with other high-risk tumor features have greater risks for cancer recurrence and benefit from subsequent surgery. © 2014 AGA Institute. Source

Asano M.,Hokkaido University | Yamamoto T.,Nayoro City University | Tsuruta T.,Hokkaido University | Nishimura N.,Nayoro City University | Sonoyama K.,Hokkaido University
Development Growth and Differentiation | Year: 2015

Small intestinal epithelium is a self-renewing system in which the entire sequence of cell proliferation, differentiation, and removal is coupled to cell migration along the crypt-villus axis. We examined whether dual labeling with different thymidine analogues, 5-bromo-2'-deoxyuridine (BrdU) and 5-ethynyl-2'-deoxyuridine (EdU), can be used to estimate cell migration rates on the villi of small intestines in rats. Rats received a single intraperitoneal injection of BrdU and EdU within a time interval, and signals in tissue sections were examined by immunohistochemistry and the "click" reaction, respectively. We successfully observed BrdU- and EdU-positive cells on the epithelium with no cross-reaction. In addition, we observed an almost complete overlapping of BrdU- and EdU-positive cells in rats administered simultaneously with BrdU and EdU. By calculating the cell migration rate by dividing the distance between the median cell positions of the distribution of BrdU- and EdU-positive cells by the time between the injection of BrdU and EdU, we estimated approximately 9 and 5 μm/h for the cell migration rates on the villi in the jejunum and ileum, respectively. We propose that dual labeling with BrdU and EdU within a time interval, followed by detecting with immunohistochemistry and the click reaction, respectively, is useful to estimate accurately the cell migration rate in the intestinal epithelium in a single animal. © 2014 Japanese Society of Developmental Biologists. Source

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