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Fu S.-B.,Peking Union Medical College | Yang J.-S.,Peking Union Medical College | Cui J.-L.,Peking Union Medical College | Feng X.,Navy Medical Research Institute | Sun D.-A.,Peking Union Medical College
Chemical and Pharmaceutical Bulletin | Year: 2011

Endophytic fungi were used not only for their producing bioactive products but also for their ability to transform natural compounds. An endophytic fungus, isolated from medicinal plant Huperzia serrata, was identified as Umbelopsis isabellina based on the internal transcribed spacer of ribosomal DNA (rDNA-ITS) region. It was used to transform ursolic acid (1), a pentacyclic triterpene. Incubation of ursolic acid with U. isabellina afforded three products, 3β-hydroxy-urs-11-en-28,13-lactone (2), 3β,7β-dihydroxy-urs-11- en-28,13-lactone (3), 1β,3β-dihydroxy-urs-11-en-28,13-lactone (4). Although product 2 was a known compound, it was first obtained by microbial transformation. Products 3 and 4 were new compounds. The structural elucidation of the three compounds was achieved mainly by the 1D- and 2D-NMR, MS, IR data. The endophytic fungus U. isabellina can hydroxyate the C12-C13 double bond at position 13 of ursolic acid 1 and form a five-member lactone effectively. In the meantime, this fungus can also introduce the hydroxyl group at C-1 or C-7 of ursolic acid 1. © 2011 Pharmaceutical Society of Japan. Source


Feng X.,Navy Medical Research Institute | Lu Y.-H.,East China University of Science and Technology | Liu Z.,Navy Medical Research Institute | Liu Z.,Shanghai Ocean University | And 4 more authors.
Journal of Asian Natural Products Research | Year: 2016

The pentacyclic triterpenoid corosolic acid was metabolized by Cunninghamella echinulata CGMCC 3.2000 to its C-24 aldehyde group metabolite and five other hydroxylated metabolites: madasiatic acid (2), 2α, 3β, 7β-trihydroxyurs-12-en-28-oic acid (3), 2α, 3β, 15α-trihydroxyurs-12-en-28-oic acid (4), 2α, 3β, 6β, 7β-tetrahydroxyurs-12-en-28-oic acid (5), 2α, 3β, 7β, 15α-tetrahydroxyurs-12-en-28-oic acid (6), and 2α, 3β,7β-trihydroxy-24-al-urs-12-en-28-oic acid (7); compounds 3, 5, and 7 were new compounds. The α-glucosidase inhibitory effects of the metabolites were also evaluated. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source


Fang J.-J.,Tongji University | Fang J.-J.,Center for the Technology Research and Training on Household Waste in Small Towns and Rural Area | Zhang H.,Tongji University | Zhang H.,Navy Medical Research Institute | And 6 more authors.
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2015

Seven categories of odorous pollutants released from the collection and transportation process of municipal solid waste (MSW), including ammonia, sulfur compounds, terpenes, aromatics, alcohols, volatile fatty acids (VFAs) and carbonyls, were quantified through different analytical methods. Based on the field data, the compounds mainly causing malodour were assessed and identified. The cancer and non-cancer risk of odor exposure in the circumstance were corrsesponingly estimated by the health risk model. The uncertainties of risk value were estimated by Monte Carlo simulation. The concentration of odorous pollutants in the transfer station was one order of magnitude higher than that in the dust bin, which was closedly associated with the degradation of MSW. The concentration of alcohols increased stably with the extension of transportation chain, whereas the concentrations of VFAs and aromatics increased with fluctuation, and peaked in the bulk transfer station and in the container transfer station (131 μg/m3, 711 μg/m3 respectively). The major odorous compounds at the early stage of transportation were carbonyls, whereas at the end stage were sulfur compounds and VFAs.The adults' total cancer risk from odor pollution in the bulk transfer station was 2.64×10-5, whereas the total non-cancer risk was 3.01in the bulk transfer sstation and 1.22 in the container transfer station, which was closed to or exceeded the maximum acceptable risk levels recommended by US EPA. The risk values in the other transportation points were within the acceptable range. ©, 2015, Chinese Society for Environmental Sciences. All right reserved. Source


Li D.-P.,Tianjin University of Science and Technology | Feng X.,Navy Medical Research Institute | Chu Z.-Y.,Navy Medical Research Institute | Guo F.-F.,Tianjin University of Science and Technology | Zhang Z.-S.,Tianjin University of Science and Technology
Journal of Asian Natural Products Research | Year: 2013

The growing cultures of Fusarium equiseti CGMCC 3.3658 and Gliocladium catenulatum CGMCC 3.3655 were used for the first time in the structural modification of corosolic acid (1). Four new metabolites were obtained. F. equiseti CGMCC 3.3658 converted 1 into 2α,3β,15α-trihydroxyurs- 12-en-28-oic acid (2) and 2α,3β,7β,15α-tetrahydroxyurs-12- en-28-oic acid (3). G. catenulatum CGMCC 3.3655 transformed 1 into 2α,21β-dihydroxy-A-homo-3α-oxours-12-en-28-oic acid (4), and 2α,3α,21β-trihydroxyurs-12-en-28-oic acid (5). The structures of four metabolites were determined by 1H NMR, 13C NMR, DEPT, HSQC, HMBC, and NOESY spectral data. Cytotoxicities in vitro of corosolic acid and four metabolites against three tumor cell lines, viz. Hela, A549, and SGC-7901, were evaluated by the classical 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) colorimetric assay. © 2013 Copyright Taylor and Francis Group, LLC. Source


Guo F.-F.,Tianjin University of Science and Technology | Feng X.,Navy Medical Research Institute | Chu Z.-Y.,Navy Medical Research Institute | Li D.-P.,Tianjin University of Science and Technology | And 2 more authors.
Journal of Asian Natural Products Research | Year: 2013

Asiatic acid (1), a major pentacyclic triterpene of Centella asiatica, was subjected to transformation by Penicillium lilacinum ACCC 31890, Fusarium equiseti CGMCC 3.3658, and Streptomyces griseus CGMCC 4.18 strains. Incubation of asiatic acid with P. lilacinum ACCC 31890 and F. equiseti CGMCC 3.3658 gave an identical product: 2α,3β,15α,23-tetrahydroxyurs-12-en-28-oic acid (2). Biotransformation of asiatic acid by S. griseus CGMCC 4.18 resulted in three derivatives: 2α,3β,21β,23-tetrahydroxyurs-12-en-28-oic acid (3), 2α,3β,23-trihydroxyurs-12-en-28, 30-dioic acid (4), and 2α,3β,23,30-tetrahydroxyurs-12-en-28-oic acid (5). The structures of those derivatives were deduced from their spectral data. Products (2), (3), and (4) were new compounds. In addition, the in vitro cytotoxicities of those derivatives along with 1 were evaluated with several human cancer cell lines. © 2013 Copyright Taylor and Francis Group, LLC. Source

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