Navy Medical Research Institute

Shanghai, China

Navy Medical Research Institute

Shanghai, China
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Fu S.-B.,Peking Union Medical College | Yang J.-S.,Peking Union Medical College | Cui J.-L.,Peking Union Medical College | Feng X.,Navy Medical Research Institute | Sun D.-A.,Peking Union Medical College
Fitoterapia | Year: 2011

The structural modification of ursolic acid by an endophytic fungus Pestalotiopsis microspora, isolated from medicinal plant Huperzia serrata was reported for the first time. The structure diversity was very important for the SAR study of ursolic acid and its derivatives. Incubation of ursolic acid 1 with P. microspora afforded four metabolites: 3-oxo-15α, 30-dihydroxy-urs-12- en-28-oic acid (2), 3β, 15α-dihydroxy-urs-12-en-28-oic acid (3), 3β, 15α, 30- trihydroxy-urs-12-en-28-oic acid (4) and 3,4-seco-ursan-4,30-dihydroxy-12-en-3,28-dioic acid (5). All products were new compounds and their structures elucidation was mainly based on the spectroscopic data. © 2011 Elsevier B.V.


Guo F.-F.,Tianjin University of Science and Technology | Feng X.,Navy Medical Research Institute | Chu Z.-Y.,Navy Medical Research Institute | Li D.-P.,Tianjin University of Science and Technology | And 2 more authors.
Journal of Asian Natural Products Research | Year: 2013

Asiatic acid (1), a major pentacyclic triterpene of Centella asiatica, was subjected to transformation by Penicillium lilacinum ACCC 31890, Fusarium equiseti CGMCC 3.3658, and Streptomyces griseus CGMCC 4.18 strains. Incubation of asiatic acid with P. lilacinum ACCC 31890 and F. equiseti CGMCC 3.3658 gave an identical product: 2α,3β,15α,23-tetrahydroxyurs-12-en-28-oic acid (2). Biotransformation of asiatic acid by S. griseus CGMCC 4.18 resulted in three derivatives: 2α,3β,21β,23-tetrahydroxyurs-12-en-28-oic acid (3), 2α,3β,23-trihydroxyurs-12-en-28, 30-dioic acid (4), and 2α,3β,23,30-tetrahydroxyurs-12-en-28-oic acid (5). The structures of those derivatives were deduced from their spectral data. Products (2), (3), and (4) were new compounds. In addition, the in vitro cytotoxicities of those derivatives along with 1 were evaluated with several human cancer cell lines. © 2013 Copyright Taylor and Francis Group, LLC.


Yu F.,Navy Medical Research Institute | Guo Y.,Shanghai University | Wang H.,Changhai Hospital | Feng J.,Shanghai University | And 4 more authors.
BMC Cancer | Year: 2016

Background: To summarize the relationship between type 2 diabetes mellitus (T2DM) and risk of colorectal adenomas (CRA), we performed a meta-analysis of observational studies. Methods: To find studies, we searched PubMed, Embase, the Cochrane Library, Web of Science and conference abstracts and related publications for American Society of Clinical Oncology and the European Society of Medical Oncology. Studies that reported relative risks (RRs) or odds ratios (ORs) with 95% confidence intervals (CIs) for the association between T2DM and risk of CRA were included. The meta-analysis assessed the relationships between T2DM and risk of CRA. Sensitivity analyses were performed in two ways: (1) by omitting each study iteratively and (2) by keeping high-quality studies only. Publication bias was detected by Egger's and Begg's tests and corrected using the trim and fill method. Results: This meta-analysis included 17 studies with 28,999 participants and 6798 CRA cases. We found that T2DM was a risk factor for CRA (RR: 1.52; 95% CI: 1.29-1.80), and also for the advanced adenoma (RR: 1.41; 95% CI: 1.06-1.87). Patients with existing T2DM (RR: 1.56; 95 % CI: 1.16-2.08) or newly diagnosed T2DM (RR: 1.51; 95% CI: 1.16-1.97) have a risk of CRA. Similar significant results were found in retrospective studies (RR: 1.57; 95 % CI: 1.30-1.89) and population based cross-sectional studies (RR: 1.46; 95 % CI: 1.21-1.89), but not in prospective studies (RR: 1.27; 95 % CI: 0.77-2.10). Conclusions: Our results suggested that T2DM plays a risk role in the risk of developing CRA. Consequently, medical workers should increase the rate of CRA screening for T2DM patients so that they can benefit from behavioural interventions that can help prevent the development of colorectal cancer. Additional, large prospective cohort studies are needed to make a more convincing case for these associations. © 2016 Yu et al.


Fu S.-B.,Peking Union Medical College | Yang J.-S.,Peking Union Medical College | Cui J.-L.,Peking Union Medical College | Feng X.,Navy Medical Research Institute | Sun D.-A.,Peking Union Medical College
Chemical and Pharmaceutical Bulletin | Year: 2011

Endophytic fungi were used not only for their producing bioactive products but also for their ability to transform natural compounds. An endophytic fungus, isolated from medicinal plant Huperzia serrata, was identified as Umbelopsis isabellina based on the internal transcribed spacer of ribosomal DNA (rDNA-ITS) region. It was used to transform ursolic acid (1), a pentacyclic triterpene. Incubation of ursolic acid with U. isabellina afforded three products, 3β-hydroxy-urs-11-en-28,13-lactone (2), 3β,7β-dihydroxy-urs-11- en-28,13-lactone (3), 1β,3β-dihydroxy-urs-11-en-28,13-lactone (4). Although product 2 was a known compound, it was first obtained by microbial transformation. Products 3 and 4 were new compounds. The structural elucidation of the three compounds was achieved mainly by the 1D- and 2D-NMR, MS, IR data. The endophytic fungus U. isabellina can hydroxyate the C12-C13 double bond at position 13 of ursolic acid 1 and form a five-member lactone effectively. In the meantime, this fungus can also introduce the hydroxyl group at C-1 or C-7 of ursolic acid 1. © 2011 Pharmaceutical Society of Japan.


Feng X.,Peking Union Medical College | Feng X.,Navy Medical Research Institute | Zou Z.-M.,Peking Union Medical College | Chu Z.-Y.,Navy Medical Research Institute | Sun D.-A.,Peking Union Medical College
Chinese Journal of Natural Medicines | Year: 2011

Microbial transformation of taxanes has provided new derivatives, some of which possess potential pharmacological activity. And microbial transformation can also be an efficient way to prepare drug metabolites. This article reviews the microbial transformation of taxanes from 1996 to 2010. © 2011 China Pharmaceutical University.


Li D.-P.,Tianjin University of Science and Technology | Feng X.,Navy Medical Research Institute | Chu Z.-Y.,Navy Medical Research Institute | Guo F.-F.,Tianjin University of Science and Technology | Zhang Z.-S.,Tianjin University of Science and Technology
Journal of Asian Natural Products Research | Year: 2013

The growing cultures of Fusarium equiseti CGMCC 3.3658 and Gliocladium catenulatum CGMCC 3.3655 were used for the first time in the structural modification of corosolic acid (1). Four new metabolites were obtained. F. equiseti CGMCC 3.3658 converted 1 into 2α,3β,15α-trihydroxyurs- 12-en-28-oic acid (2) and 2α,3β,7β,15α-tetrahydroxyurs-12- en-28-oic acid (3). G. catenulatum CGMCC 3.3655 transformed 1 into 2α,21β-dihydroxy-A-homo-3α-oxours-12-en-28-oic acid (4), and 2α,3α,21β-trihydroxyurs-12-en-28-oic acid (5). The structures of four metabolites were determined by 1H NMR, 13C NMR, DEPT, HSQC, HMBC, and NOESY spectral data. Cytotoxicities in vitro of corosolic acid and four metabolites against three tumor cell lines, viz. Hela, A549, and SGC-7901, were evaluated by the classical 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) colorimetric assay. © 2013 Copyright Taylor and Francis Group, LLC.


Liu Z.,Shanghai Ocean University | Lu Y.-H.,East China University of Science and Technology | Feng X.,East China University of Science and Technology | Feng X.,Navy Medical Research Institute | And 3 more authors.
Journal of Asian Natural Products Research | Year: 2016

The pentacyclic triterpenoid hederagenin (1) was subjected to biotransformation by Cunninghamella echinulate CGMCC 3.2000, Mucor subtilissimus CGMCC 3.2454 and Pseudomonas oleovorans CGMCC 1.1641. Three metabolites were obtained. On the basis of nuclear magnetic resonance and high-resolution mass spectral analyses, their structures were characterized as 3β, 23-dihydroxyolean-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester (2), 3β, 15α, 23-trihydroxyolean-12-en-28-oic acid (3), 1β, 3β, 23-trihydroxyolean-12-en-28-oic acid (4), and metabolite (3) was a new compound. This was the first report on the biotransformation of hederagenin. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Feng X.,Navy Medical Research Institute | Luan J.,Navy Medical Research Institute | Guo F.F.,Tianjin University of Science and Technology | Li D.P.,Tianjin University of Science and Technology | Chu Z.Y.,Navy Medical Research Institute
Journal of Molecular Catalysis B: Enzymatic | Year: 2012

The pentacyclic triterpenoid maslinic acid (1) was bioconverted by Cunninghamella blakesleana CGMCC 3.910, four metabolites were obtained. On the basis of nuclear magnetic resonance and high-resolution mass spectral analyses, their structures were identified as 2α,3β,7β-trihydroxyolean-12- en-28-oic acid (2), 2α,3β,15α-trihydroxyolean-12-en-28-oic acid (3), 2α,3β,7β,15α-tetrahydroxyolean-12-en-28-oic acid (4), 2α,3β,7β,13β-tetrahydroxyolean-11-en-28-oic acid (5), all of the metabolites were new compounds. The metabolic pathway was also investigated. This was the first report on the biotransformation of maslinic acid. © 2012 Elsevier B.V.


Feng X.,Navy Medical Research Institute | Lu Y.-H.,East China University of Science and Technology | Liu Z.,Navy Medical Research Institute | Liu Z.,Shanghai Ocean University | And 4 more authors.
Journal of Asian Natural Products Research | Year: 2016

The pentacyclic triterpenoid corosolic acid was metabolized by Cunninghamella echinulata CGMCC 3.2000 to its C-24 aldehyde group metabolite and five other hydroxylated metabolites: madasiatic acid (2), 2α, 3β, 7β-trihydroxyurs-12-en-28-oic acid (3), 2α, 3β, 15α-trihydroxyurs-12-en-28-oic acid (4), 2α, 3β, 6β, 7β-tetrahydroxyurs-12-en-28-oic acid (5), 2α, 3β, 7β, 15α-tetrahydroxyurs-12-en-28-oic acid (6), and 2α, 3β,7β-trihydroxy-24-al-urs-12-en-28-oic acid (7); compounds 3, 5, and 7 were new compounds. The α-glucosidase inhibitory effects of the metabolites were also evaluated. © 2016 Informa UK Limited, trading as Taylor & Francis Group


PubMed | Navy Medical Research Institute
Type: Journal Article | Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2010

To study changes of function of transmitter glycine in nitrogen narcosis.Synaptosomes of rat spinal cord were prepared. Glycine uptake of synaptosomes of rat spinal cord in 0.7 MPa (7ATA) hyperbaric air pressure was observed by the methods of isotope.Glycine uptake slowed down and took a longer period of time to reach saturation in 0.7 MPa (7ATA). The maximum glycine uptake was lessened. Vm was diminished, but Km was increased. Vm rose in 0.7 MPa (7ATA) when corticosterone was added.When nitrogen narcosis arose in 0.7 MPa (7ATA), the function of transporters of glycine re-uptake was reduced, the affinity of glycine for transporters subsided. Corticosterone was conductive to the recovery of the function of glycine transporters of high affinity.

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