Haidian District, China
Haidian District, China

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Lu W.,General Navy Hospital of PLA | Li Z.,General Navy Hospital of PLA | Tian Z.,General Navy Hospital of PLA | Jia B.,General Navy Hospital of PLA | Zeng Y.,Beijing University of Technology
Neurosurgery Quarterly | Year: 2013

OBJECTIVE: To investigate the effect of embryonic neural stem cell (NSC) transplantation on cerebral infarction sequelae. METHODS: From June 2004 to June 2008, embryonic NSCs were implanted into the ipsilateral basal ganglia region in 21 patients with cerebral infarction, using stereotactic technology. The function independence measure (FIM) was used to evaluate motor function and quality of life in the 21 patients before treatment, and at 1 and 6 months after treatment. FIM was regarded as effective if elevated by ≥1 point. RESULTS: Clinical symptoms improved at an effective rate of 80.95% (17/21 cases). The FIM scores were 93.76±6.83 at 1 month after treatment, which was significantly higher than 91.21±6.42 before treatment; however, they were significantly lower than 97.45±7.56 at 6 months after treatment. Patients undergoing embryonic NSC transplantation had no serious complications. CONCLUSIONS: NSC transplantation can improve cerebral infarction sequelae including motor function and living quality in patients to some extent. However, the long-term effect of NSC transplantation still needs further study. Copyright © 2013 by Lippincott Williams & Wilkins.


Mai S.,Beijing Institute of Biotechnology | Qu X.,General Navy Hospital of PLA | Li P.,Beijing Institute of Biotechnology | Ma Q.,Beijing Institute of Biotechnology | And 2 more authors.
Biochemical and Biophysical Research Communications | Year: 2016

RBM39, also known as splicing factor HCC1.4, acts as a transcriptional coactivator for the steroid nuclear receptors JUN/AP-1, ESR1/ER-α and ESR2/ER-β. RBM39 is involved in the regulation of the transcriptional responses of these steroid nuclear receptors and promotes transcriptional initiation. In this paper, we report that RBM39 interacts with the nonreceptor tyrosine kinase c-Abl. Both the Src homology (SH) 2 and SH3 domains of c-Abl interact with RBM39. The major tyrosine phosphorylation sites on RBM39 that are phosphorylated by c-Abl are Y95 and Y99, as demonstrated by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) and mutational analysis. c-Abl was shown boost the transcriptional coactivation activity of RBM39 for ERα and PRβ in a tyrosine kinase-dependent manner. The results suggest that mammalian c-Abl plays an important role in steroid hormone receptor-mediated transcription by regulating RBM39. © 2016 Elsevier Inc.


Mai S.,Beijing Institute of Biotechnology | Qu X.,General Navy Hospital of PLA | Li P.,Beijing Institute of Biotechnology | Ma Q.,Beijing Institute of Biotechnology | And 2 more authors.
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms | Year: 2016

Background: RBM39 is a serine/arginine-rich RNA-binding protein that is highly homologous to the splicing factor U2AF65. However, the role of RBM39 in alternative splicing is poorly understood. Methods: In this study, RBM39-mediated global alternative splicing was investigated using RNA-Seq and genome-wide RBM39-RNA interactions were mapped via cross-linking and immunoprecipitation coupled with deep sequencing (CLIP-Seq) in wild-type and RBM39-knockdown MCF-7 cells. Results: RBM39 was involved in the up- or down-regulation of the transcript levels of various genes. Hundreds of alternative splicing events regulated by endogenous RBM39 were identified. The majority of these events were cassette exons. Genes containing RBM39-regulated alternative exons were found to be linked to G2/M transition, cellular response to DNA damage, adherens junctions and endocytosis. CLIP-Seq analysis showed that the binding site of RBM39 was mainly in proximity to 5' and 3' splicing sites. Considerable RBM39 binding to mRNAs encoding proteins involved in translation was observed. Of particular importance, ~. 20% of the alternative splicing events that were significantly regulated by RBM39 were similarly regulated by U2AF65. Conclusions: RBM39 is extensively involved in alternative splicing of RNA and helps regulate transcript levels. RBM39 may modulate alternative splicing similarly to U2AF65 by either directly binding to RNA or recruiting other splicing factors, such as U2AF65. General significance: The current study offers a genome-wide view of RBM39's regulatory function in alternative splicing. RBM39 may play important roles in multiple cellular processes by regulating both alternative splicing of RNA molecules and transcript levels. © 2016 Elsevier B.V.

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