Navsari Agricultural University
Gujarat, India

Navsari Agricultural University is an agricultural university at Navsari in the Indian state of Gujarat. It was part of Gujarat Agricultural University until May 2004. Wikipedia.

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Mehta V.N.,Sardar Vallabhbhai National Institute of Technology, Surat | Jha S.,Navsari Agricultural University | Singhal R.K.,Bhabha Atomic Research Center | Kailasa S.K.,Sardar Vallabhbhai National Institute of Technology, Surat
New Journal of Chemistry | Year: 2014

We have synthesized biocompatible fluorescent carbon dots (CDs) by a one-step hydrothermal method using Solanum tuberosum (potato) as a raw material. The CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), dynamic light scattering (DLS) and high-resolution transmission electron microscopic (HR-TEM) techniques. We found that the carbonization of potato at ∼170 °C for 12 h produces highly fluorescent CDs of 0.2-2.2 nm size. The synthesized CDs are well dispersed in water and exhibited strong blue and bright blue emissions under UV illumination (λex = 302, 365 nm). The CDs showed a strong emission peak at 455 nm at an excitation wavelength of 374 nm. The CDs acted as fluorescent probes for multicolor (blue, green and red) imaging of HeLa cells and the CDs did not induce cell death, which indicates that the CDs are biocompatible and nontoxic to HeLa cells. Therefore, the CDs can be used as probes for cell-imaging applications in vitro and in vivo. This journal is © 2014 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.

Mehta V.N.,Sardar Vallabhbhai National Institute of Technology, Surat | Jha S.,Navsari Agricultural University | Kailasa S.K.,Sardar Vallabhbhai National Institute of Technology, Surat
Materials Science and Engineering C | Year: 2014

We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ~ 3 nm and showed bright blue fluorescence under UV-light (λex = 365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae). © 2014 Elsevier B.V.

Jain R.,Navsari Agricultural University | Adhikary H.,M. S. University of Baroda | Jha S.,Navsari Agricultural University | Jha A.,Ashok and Rita Patel Institute of Integrated Study Research in Biotechnology and Allied science | Kumar G.N.,M. S. University of Baroda
Microbial Biotechnology | Year: 2012

Arsenite-tolerant bacteria were isolated from an organic farm of Navsari Agricultural University (NAU), Gujarat, India (Latitude: 20°55′39.04″N; Longitude: 72°54′6.34″E). One of the isolates, NAU-1 (aerobic, Gram-positive, non-motile, coccobacilli), was hyper-tolerant to arsenite (AsIII, 23mM) and arsenate (AsV, 180mM). 16S rRNA gene of NAU-1 was 99% similar to the 16S rRNA genes of Rhodococcus (Accession No. HQ659188). Assays confirmed the presence of membrane bound arsenite oxidase and cytoplasmic arsenate reductase in NAU-1. Genes for arsenite transporters (arsB and ACR3(1)) and arsenite oxidase gene (aoxB) were confirmed by PCR. Arsenite oxidation and arsenite efflux genes help the bacteria to tolerate arsenite. Specific activities of antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase and glutathione S-transferase) increased in dose-dependent manner with arsenite, whereas glutathione reductase activity decreased with increase in AsIII concentration. Metabolic studies revealed that Rhodococcus NAU-1 produces excess of gluconic and succinic acids, and also activities of glucose dehydrogenase, phosphoenol pyruvate carboxylase and isocitrate lyase were increased, to cope with the inhibited activities of glucose-6-phosphate dehydrogenase, pyruvate dehydrogenase and α-ketoglutarate dehydrogenase enzymes respectively, in the presence of AsIII. Enzyme assays revealed the increase in direct oxidative and glyoxylate pathway in Rhodococcus NAU-1 in the presence of AsIII. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Jha S.,M. S. University of Baroda | Jha S.,Navsari Agricultural University | Chattoo B.B.,M. S. University of Baroda
Transgenic Research | Year: 2010

Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2 was not accompanied by an induction of pathogenesis-related (PR) gene expression, suggesting that the expression of Rs-AFP2 directly inhibits the pathogens. Here, we demonstrate that transgenic rice plants expressing the Rs-AFP2 gene show enhanced resistance to M. oryzae and R. solani, two of the most important pathogens of rice. © 2009 Springer Science+Business Media B.V.

Thakur S.,M. S. University of Baroda | Jha S.,Navsari Agricultural University | Chattoo B.B.,M. S. University of Baroda
BMC Research Notes | Year: 2011

Background: Ricinus communis is an industrially important non-edible oil seed crop, native to tropical and subtropical regions of the world. Although, R. communis genome was assembled in 4X draft by JCVI, and is predicted to contain 31,221 proteins, the function of most of the genes remains to be elucidated. A large amount of information of different aspects of the biology of R. communis is available, but most of the data are scattered one not easily accessible. Therefore a comprehensive resource on Castor, Castor DB, is required to facilitate research on this important plant. Findings. CastorDB is a specialized and comprehensive database for the oil seed plant R. communis, integrating information from several diverse resources. CastorDB contains information on gene and protein sequences, gene expression and gene ontology annotation of protein sequences obtained from a variety of repositories, as primary data. In addition, computational analysis was used to predict cellular localization, domains, pathways, protein-protein interactions, sumoylation sites and biochemical properties and has been included as derived data. This database has an intuitive user interface that prompts the user to explore various possible information resources available on a given gene or a protein. Conclusion: CastorDB provides a user friendly comprehensive resource on castor with particular emphasis on its genome, transcriptome, and proteome and on protein domains, pathways, protein localization, presence of sumoylation sites, expression data and protein interacting partners. © 2011 Chattoo et al; licensee BioMed Central Ltd.

Sharma A.,Central Agricultural University | Sharma A.,Central Institute of Fisheries Education | Deo A.D.,Central Agricultural University | Tandel Riteshkumar S.,Navsari Agricultural University | And 2 more authors.
Fish and Shellfish Immunology | Year: 2010

The present study evaluated the efficacy of dietary doses of Withania somnifera (Ashwagandha) root powder on immunological parameters and disease resistance against the bacterial pathogen Aeromonas hydrophila infections in Indian major carp, Labeo rohita fingerlings. Fishes were fed with dry diet containing 0gkg-1 (control), 1gkg-1 (T1), 2gkg-1 (T2) and 3gkg-1 (T3) W. somnifera root powder for 42 days. Immunological (NBT level, Phagocytic activity, total immunoglobulin and lysozyme activity) parameters of fishes were examined at 0 days,14 days, 28 days and 42 days of feeding. Fishes were challenged with A. hydrophila 42 days post feeding and mortalities (%) were recorded over 14 days post-infection. The results demonstrate that fishes fed with W. somnifera root showed enhanced NBT level, Phagocytic activity, total Immunoglobulin level and lysozyme activity (p<0.05) compared with the control group. The survivability was higher in experimental diets than the control group. Dietary W. somnifera at the level of 2gkg-1 showed significantly (P<0.05) higher protection (RPS 42.85±0.65%) against A. hydrophila infection than control. The results suggest that the W. somnifera root powder have a stimulatory effect on immunological parameters and increases disease resistance in L. rohita fingerlings against A. hydrophila infection. © 2010 Elsevier Ltd.

Shah R.,Uka Tarsadia University | Jha S.,Navsari Agricultural University
Chemistry and Ecology | Year: 2013

Twenty five different arsenite (AsIII) oxidising bacteria were isolated from industrial effluent (Bilimora, Gujarat Industrial Development Corporation (GIDC) Gujarat, India). The most efficient arsenite oxidiser and arsenite hyper-tolerant bacterial isolate which could tolerate up to 15 mM AsIII was selected and 16S rRNA gene sequence analysis was performed. It showed 98% identity of the 16S rRNA genes of genus Alishewanella (Accession no.: HQ659190). Higher activities of arsenite oxidase enzymes in the bacterium were localised on membrane. The GIDC-5 bacterial strain was found to be susceptible to most of the common antibiotics. The optimum growth in the presence of metalloid was observed at temperature 37°C and pH 4, whereas in the absence of metalloid the optimum pH was 8. The result suggested that, Alishewanella sp. strain GIDC-5 demonstrated hyper-tolerant to arsenite because of its ability to overcome the toxic effects of arsenite by converting it to less toxic forms (arsenate). © 2013 Copyright Taylor and Francis Group, LLC.

Dabas V.S.,Navsari Agricultural University
Buffalo Bulletin | Year: 2013

This communication reports a case of surgical management of colonic faecolith in a she buffalo.

Virdia H.M.,Navsari Agricultural University | Patel C.L.,Navsari Agricultural University
Indian Journal of Agronomy | Year: 2010

A field experiment was conducted at Navsari during 2002-2006 to study the influence of integrated nutrient management (INM) for sustained sugarcane plant-ratoon production system. Integrated use of various organics (FYM, pressmud, vermicompost and biofertilizers) with recommended dose of NPK fertilizers (RDF) to sugarcane plant crop followed by trash incorporation and green manuring with NPK and bio-fertilizers to ratoon sugarcane was studied. The results revealed that integrated use of FYM @25% of recommended dose of N (RDN) with RDF (250-125-125 kg N-P-K/ha) and biofertilizer (Azotobactor and phosphate solubilizing bacteria (PSB) to plant crop followed by 10 t/ha trash incorporation with bio-fertilizer inoculation + RDF (300-6.25-125 kg N-P-K/ha) application increased cane (13.3 to 30.6 t/ha) and commercial cane sugar (0.5 to 1.6 tonne/ha) yield. However, the magnitude of variation in cane yield was marginal when total N was applied through fertilizer alone. Hence, it was possible to save 25% N when organics was applied along with bio-fertilizers Azatobacter + PSB.

Gupta S.,Navsari Agricultural University | Savalia C.V.,Navsari Agricultural University
Veterinary World | Year: 2012

Biotechnological achievements of recent years have emerged as powerful tool to improve quality attributes of livestock products including milk and meat products. Biotechnological approaches can be employed for improving productivity, economy, physicochemical and nutritional attributes of a wide range of livestock products. The target areas of biotechnological research in the field of livestock products can be envisaged as production of high yielding food animal, improvement in quality of their products, enhanced production of natural food grade preservatives, efficient byproduct utilization and so forth. Many of the biotechnological techniques can be explored in the area of quality assurance programmes, which would be of great help to produce livestock products of assured quality and public health safety.

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