Naval Medical Research Institute of PLA

Shanghai, China

Naval Medical Research Institute of PLA

Shanghai, China
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Zhao Y.-N.,Shanghai Ocean University | He Y.,Naval Medical Research Institute of PLA | Shen X.-R.,Naval Medical Research Institute of PLA | Zhang J.-L.,Naval Medical Research Institute of PLA | And 4 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2017

Objects To study the protective effects of cimetidine against oxidative stress in rats induced by cumulative low-dose irradiation. Methods Sixty SD rats were randomly divided into 6 groups (10 each): normal control group, model control group, lentinan group [89mg/(kg·d)] and 3 dose groups of cimetidine. After oral administration, all the rats were exposed to γ-ray irradiation 8 hours/day for 12 days, and sacrificed on the 13th day. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and the content of malondialdehyde (MDA) in serum, liver, thymus and spleen were determined. By using the superoxide anion radical system, hydroxyl radical system, H2O2 radical system, oxidation system of linoleic acid induced by alkane radical system and diphenyl picryl hydrazinyl radical (DPPH) radical system, the antioxidation activities of cimetidine were detected. Results The activities of SOD in liver and thymus decreased significantly, the GPx activity in serum, liver and spleen decreased significantly and MDA level in serum, liver and spleen increased significantly after 0.3Gy cumulative ionizing radiation. Cimetidine enhanced the activities of antioxidant enzymes in serum and organs, and reduced the MDA level. In a certain concentration range, cimetidine had different scavenging effects onto these radical systems, and showed good performance in hydroxyl radical. Conclusion Cimetidine can effectively ameliorate the oxidative stress from low-dose cumulative irradiation by scavenging free radicals, increase the activity of antioxidant enzymes and reduce the content of lipid peroxidation products, thus presents a potential radio protective effect. © 2017, People's Military Medical Press. All rights reserved.


Chu Z.-Y.,Naval Medical Research Institute of PLA | Shen Y.,Naval Medical Research Institute of PLA | Li T.-J.,Shanghai University | Lei C.-X.,Naval Medical Research Institute of PLA | And 3 more authors.
Chinese Journal of New Drugs | Year: 2010

Objective: To observe the effects of Suxiaokangyun capsules on cardiovascular, respiratory and nervous system in Beagle dogs and mice. Methods: Beagle dogs received single doses of Suxiaokangyun capsules (5, 10 and 20 mg·kg-1); then the BP, ECG, respiratory rate, and respiratory rhythm were observed. Mice received sinlge doses of Suxiaokangyun capsules (20, 40 and 80 mg·kg-1), and then the spontaneous activities and pull-down ability were observed. Results: The respiratory rate increased between 60-120 min, while the BP, ECG and cardiac rhythm did not obviously change after single ig doses of Suxiaokangyun capsules in Beagle dogs. The spontaneous activities increased between 60-120 min, while the pull-down and synergism of pentobarbital sodium did not obviously change after single ig doses of Suxiaokangyun capsule in mice. Conclusion: Suxiaokangyun capsule has mild effects on respiratory and nervous system; it increases the respiratory rate (20 mg·kg-1) in dogs and spontaneous activities (80 mg·kg-1) in mice.


Xia Y.-B.,101 Hospital of PLA | Lin S.-L.,Naval Medical Research Institute of PLA | Geng C.-J.,101 Hospital of PLA | Wang J.-J.,Shanghai University
Chinese Journal of Medical Imaging Technology | Year: 2010

Objective: To observe chest X-ray changes of rabbits injuried by underwater shock wave. Methods: Sixty-one rabbits were placed 2 m or 6 m underwater with consciousness. Thoracic injuries model rabbits were established by 50 g and 500 g TNT spherical explosives with different equivalent. Comparison with pathological findings, chest X-ray changes of rabbits were observed. Results: Chest X-ray changes of rabbits with thoracic injuries included lung contusion (52/61), pulmonary laceration (38/61), hematoma (32/61), hemapneumothorax (31/61) and diaphragm rupture (5/61). Pathological manifestations included scattered bleeding of alveoli (56/61), formation of thrombosis of small pulmonary artery and vein (33/61), pulmonary laceration (35/61), hematoma (32/61), diaphragm rupture (5/61). Body injury varied with different equivalents of explosive and shock wave in same experimental conditions. The changes were related to explosion impact borne by the rabbits in water, density of energy flow and depth of water. Conclusion: Chest X-ray can objectively and accurately evaluate the chest injury of rabbits caused by shock wave underwater.


He Y.,Naval Medical Research Institute of PLA | Shen X.-R.,Naval Medical Research Institute of PLA | Hou D.-Y.,Naval Medical Research Institute of PLA | Liu Y.-M.,Naval Medical Research Institute of PLA | And 5 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To explore the effect of low-dose γ-rays on the metabolites in rat serum. Methods Sixteen healthy male SD rats were randomly divided into control group and irradiated group (n=8). The rats in irradiated group were irradiated by 60Co γ-rays with a dose rate of 72mGy/h for 7 days (1 hour per day). At the 7th day after irradiation, blood samples were taken from abdominal aorta to obtain the serum. The metabolic fingerprints of serum were obtained from the two groups of rats, and 1H nuclear magnetic resonance (NMR) spectroscopy, principal component analysis (PCA) and orthogonal signal correction-partial least squares (OSC-PLS) method were used for pattern recognition, and the difference in metabolite profile between two groups was identified by SIMCA-P software. Results The rat serum 1H NMR spectra revealed a significant difference between control group and irradiated group, the OSC-PLS plots of the serum samples presented marked clustering between two groups. Compared with the control group, the content of lipid, glucose, creatine, glycine/glucose, high density lipoprotein, low density lipoprotein, very low density lipoprotein/low density lipoprotein and unsaturated fatty acid increased, while the content of lactic acid, threonine/lipid, alanine, N-acetyl glycoprotein 1, N-acetyl glycoprotein 2, saturated fatty acid and phosphatidyl choline decreased in irradiated group. Conclusion Irradiation with low-dose γ-ray could induce changes in metabolites in rat serum, concerning mainly immune function, energy metabolism, carbohydrate metabolism and lipid metabolism.


He Y.,Naval Medical Research Institute of PLA | Shen X.-R.,Naval Medical Research Institute of PLA | Qian T.-T.,Naval Medical Research Institute of PLA | Wang Q.-R.,Naval Medical Research Institute of PLA | And 5 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To investigate the effects of low dose γ-ray on the gene expression of cyclin G1 (CCNG1) in human lymphoblasts AHH-1 line and the probability of CCNG1 as bio-dosimeter for low-dose radiation. Methods Lymphoblasts of AHH-1 line were irradiated with γ-ray in the doses of 0, 0.1, 0.2, 0.5, 0.8 or 1.0 Gy. Total RNA of cells was extracted at 0, 4, 24, 48, 72 and 168h after irradiation, and real-time Q-PCR was used to detect the expression of CCNG1 gene in AHH-1 cells. The relationship between the irradiation dosage or duration and the gene expression was analyzed. Results The gene expression of CCNG1 in AHH-1 cells increased in a dose-dependent manner after irradiation, presenting a better dose-effect relationship. Time-effect curve indicated that the peak expression of CCNG1 appeared at 24h after irradiation, and then the expression decreased gradually with time, and it recovered to the level before irradiation at 168h after irradiation. Conclusions CCNG1 gene is sensitive to low dose irradiation (0-1.0Gy) with a better dose and time-effect relationship, and it may be used as a potential efficient biological dosimetry in low dose exposure risk assessment. © 2015, People’s Military Medical Press. All rights reserved.


Wang Q.-R.,Naval Medical Research Institute of PLA | Shen X.-R.,Naval Medical Research Institute of PLA | He Y.,Naval Medical Research Institute of PLA | Liu Y.-M.,Naval Medical Research Institute of PLA | And 4 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To investigate the combined effects of low-dose ionizing radiation, carbon monoxide, benzene and noise on serum metabolites and the mechanism of injury induced by these complex environmental factors in rats. Methods Sixteen adult SD rats were randomly divided into control group and exposed group (8 each). The exposed group received the combined effect every day for 7 days. At the end of experiment, sera were collected from the abdominal aorta of rats. The metabolic fingerprint of serum was obtained by 1H nuclear magnetic resonance (1H NMR) spectroscopy and determined with pattern recognition techniques of principal component analysis (PCA) and orthogonal signal correction-partial least squares (OSC-PLS). The similarities and differences in metabolic profiles between two groups were visualized by SIMCA-P software. Results The rat serum 1H NMR spectra revealed different metabolic spectra between the control group and exposed group. The OSC-PLS plots of the serum samples presented respectively marked clustering between the two groups. Compared with the control group, the contents of lipid, high density lipoprotein, glycine/glucose, N-acetyl glycoprotein 1, N-acetyl glycoprotein 2, phosphatidyl choline and unsaturated fatty acid increased, while those of lactic acid, threonine/lipid, alanine, creatine, glycerylphosphorylcholine/ trimethylamine oxide, low density lipoprotein/high density lipoprotein, low density lipoprotein, very low density lipoprotein/ low density lipoprotein, very low density lipoprotein and saturated fatty acid decreased. Conclusions Combination of low-dose ionizing radiation, carbon monoxide, benzene and noise could induce changes of serum metabolites in rats, involving in immune function, renal function and energy metabolism. The NMR-based-metabonomics method has potential of application in research on combined biological effects of the complex environmental factors. © 2015, Medical Journal of Chinese People's Liberation Army. All right reserved.


Ruiyong C.,Naval Medical Research Institute of PLA | Weibing X.,Naval Medical Research Institute of PLA | Jun L.,Naval Medical Research Institute of PLA | Jia H.,Naval Medical Research Institute of PLA | Haiting C.,Naval Medical Research Institute of PLA
Undersea and Hyperbaric Medicine | Year: 2012

High pressure nervous syndrome (HPNS) is an instinctive response of mammalian high-class nervous functions to increased hydrostatic pressure [2,5,8]. Electrophysiological activity of mammalian central nervous system (CNS), including brainstem auditory-evoked potential (BAEP), has characteristic changes under pressure. Here we recorded BAEP of 63 mice exposed to 0-4.0 MPa. The results showed that interpeak latencies between wave I and wave IV (IPL1-4) and their changes under pressures (Delta;IPL1-4) responded to increasing pressure in a biphase pattern, shortened under pressure from 0 to 0.7MPa, then prolonged later. There were signifcantly negative correlations between base IPL1-4s and ΔIPL1-4s (p < 0.01). Individual IPL1-4s were supposed to respond to increasing pressure in a relative steady pattern in accordance with its base IPL1-4s. Those with shorter-base IPL1-4 presented direct increases in IPL1-4. However, those with longer-base IPL1-4 had a decreased IPL1-4 under small to moderate pressure then rebounded later. Our results suggested that mammalian CNS functions were susceptible to small to moderate pressure, as well as a higher pressure than 1.0MPa. Mice, as a statistical mass, had an "optimum" pressure about 0.7MPa, rather than atmospheric pressure, referred as shortest IPL1-4s. An individual's response to high pressure might be relied on his base biological condition. Our results highlighted a new approach to investigate a practical strategy to medical selecting barotolerant candidates for deep divers. Diversity of individual susceptibility to hydrostatic pressure was under discussed. Underlying mechanisms of the "optimum" pressure for CNS function and its signifcance to neurophysiology remain open to further exploration. © 2012 Undersea & Hyperbaric Medical Society, Inc.


PubMed | Naval Medical Research Institute of PLA
Type: Journal Article | Journal: Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc | Year: 2012

High pressure nervous syndrome (HPNS) is an instinctive response of mammalian high-class nervous functions to increased hydrostatic pressure. Electrophysiological activity of mammalian central nervous system (CNS), including brainstem auditory-evoked potential (BAEP), has characteristic changes under pressure. Here we recorded BAEP of 63 mice exposed to 0-4.0 MPa. The results showed that interpeak latencies between wave I and wave IV (IPL1-4) and their changes under pressures (deltaIPL1-4) responded to increasing pressure in a biphase pattern, shortened under pressure from 0 to 0.7MPa, then prolonged later. There were significantly negative correlations between base IPL1-4s and deltaIPL1-4s (p < 0.01). Individual IPL1-4s were supposed to respond to increasing pressure in a relative steady pattern in accordance with its base IPL1-4s. Those with shorter-base IPL1-4 presented direct increases in IPL1-4. However, those with longer-base IPL1-4 had a decreased IPL1-4 under small to moderate pressure then rebounded later. Our results suggested that mammalian CNS functions were susceptible to small to moderate pressure, as well as a higher pressure than 1.0MPa. Mice, as a statistical mass, had an optimum pressure about 0.7MPa, rather than atmospheric pressure, referred as shortest IPL1-4s. An individuals response to high pressure might be relied on his base biological condition. Our results highlighted a new approach to investigate a practical strategy to medical selecting barotolerant candidates for deep divers. Diversity of individual susceptibility to hydrostatic pressure was under discussed. Underlying mechanisms of the optimum pressure for CNS function and its significance to neurophysiology remain open to further exploration.

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