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Lu Z.,Chinese Academy of Sciences | Lu Z.,University of Sichuan | Zhang C.,Chinese Academy of Sciences | Cui J.,Chinese Academy of Sciences | And 8 more authors.
Oncology Reports | Year: 2014

The therapeutic potential of membrane complement regulatory protein (mCRP)-neutralizing antibodies is unsatisfactory, which perhaps lies in the complex role of mCRPs in tumor occurrence and development. As a member of the mCRPs, CD46 is a transmembrane protein with a cytoplasmic domain and is implicated more in the control of the alternative complement pathway than of the classical complement pathway. Growing evidence has revealed that both the CD46 signaling pathway and microRNAs (miRNAs) play an important role in the development and progression of hepatocellular carcinoma (HCC). In the present study, we analyzed mCRP expression in different tumor tissues by employing western blotting and qPCR. To address the potential role of miRNAs in CD46 signaling, we set out to profile miRNA expression in CD46-overexpressed and -silenced HepG2 cell lines. Furthermore, bioinformatic analysis was performed to identify downstream targets of CD46 signaling. We found that the levels of CD46 expression in HCC tissues were significantly higher compared to that in the adjacent normal tissues. After complement-related gene expression profiling and unsupervised hierarchical clustering analysis of 10 HCC tissues, a total of 37 miRNAs showed significantly different expression levels before and after CD46 expression change. By bioinformatic analysis, we identified let-7b and miR-17 as downstream targets of CD46 signaling, and that the expression levels of let-7b and miR-17 were negatively correlated with that of CD46 in HepG2 cells. The present study suggests that CD46 plays an important role in HCC carcinogenesis by regulating let-7b and miR-17.

Miao Z.,Shanghai University | Zhang J.,Shanghai University | You L.,Naval General Hospital of PLA | Wang J.,Fudan University | And 14 more authors.
Bioorganic and Medicinal Chemistry | Year: 2010

Homocamptothecins (hCPTs) represents a new promising class of topoisomerase I inhibitors with enhanced stability and superior antitumor activity. Some phosphodiesters and phosphotriesters homocamptothecin derivatives were designed and synthesized based on our previous synthetic route. The cytotoxicity in vitro on three cancer cell lines and antitumor activity in vivo, and inhibitory properties of topoisomerase I of these derivatives were evaluated. Among them compounds 24e and 24f exhibited higher cytotoxic activity than IRT and the former exhibited the best antitumor activity in vivo and solution stability both at pH 7.4 and pH 3.0. © 2010 Elsevier Ltd. All rights reserved.

Wang F.,Naval General Hospital of PLA | Lu H.-X.,Naval General Hospital of PLA | Lin S.-S.,Naval General Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Rapid expansion of the dental arch is an effective way to rapidly expanse the jaw. Compared with rapid expansion, the slow expansion has higher stability and less recurrence, but the reports on the long-term stability of quad helix expansion are rare. OBJECTIVE: To retrospectively analyze the clinical effect of quad helix expansion in orthodontics. METHODS: Twenty-two subjects with dental arch stenosis in mixed dentition and early permanent dentition who experienced an expansion of at least 3 mm with quad helix appliance were selected for this study. Plaster dental casts and posteroanterior radiographs were evaluated at the beginning of the treatment (T1), at the completion of phase I quad helix expansion or full treatment (T2), and approximately 2 years following the completion of treatment (T3). The distance between two first molars was measured on the model. J point was drawn on the posteroanterior radiograph in order to measure the distance between the bilateral base bones and the molar inclination, as well as to evaluate the corrective and orthopedic effects of dental arch expansion. RESULTS AND CONCLUSION: Compared with that before expansion, the first permanent molar inclination and the distance between base bones on two sides were significant increased after quad helix expansion; there were no significant differences in the distance between two first permanent molars, first permanent molar inclination and the distance between bilateral base bones on two sides when compared after quad helix expansion and after 2-year follow-up (P > 0.05). The results indicate that the long-term effect of quad helix expansion is stable with orthopedic effect.

Liang B.,Beihang University | Liu B.,Beihang University | Zhou F.,Beihang University | Guo B.,Beihang University | And 4 more authors.
IFMBE Proceedings | Year: 2015

Body gamma knife stereotactic radiotherapy (SBRT) is an effective treatment for non–small-cell lung cancer (NSCLC). Treatment planning is both tedious and time-consuming due to its complexity. In order to address this issue, we present a memetic optimization algorithm for body gamma knife SBRT treatment planning. Different from currently available methods, the memetic approach performs the optimization on relative dosimetric distribution, which results in an enlarged search space. Taking advantage of the prior knowledge about the interaction between the focuses, a heuristic initialization strategy and a set of genetic operators are developed. During optimization, the most time consuming part is accelerated by graphic processing unit (GPU). Three typical targets arising from real patient data are used to test the efficiency of this approach. Experimental results demonstrate that the memetic optimization could generate a feasible treatment plan quickly (94.6 sec for the largest case). © Springer International Publishing Switzerland 2015.

Lu Z.,Naval General Hospital of PLA | Song Q.,Chinese Peoples Armed Police forces Academy | Yang J.,University of Sichuan | Zhao X.,Naval General Hospital of PLA | And 3 more authors.
Cellular Physiology and Biochemistry | Year: 2014

Background: Periplocin is used for treatment of rheumatoid arthritis, reinforcement of bones and tendons, palpitations or shortness of breath and lower extremity edema in traditional medicine. Our previous findings suggested that periplocin could inhibit the growth of lung cancer both in vitro and in vivo. But the biological processes and molecular pathways by which periplocin induces these beneficial effects remain largely undefined. Methods: To explore the molecular mechanisms of periplocin involved in anti-cancer activity, in the present study the protein profile changes of human lung cancer cell lines A549 in response to periplocin treatment were investigated using the proteomics approaches (2-DE combined with MS/MS). Western blot was employed to verify the changed proteins. Interactions between changed proteins were analyzed by STRING. Results: 29 down-regulated protein species named GTP-binding nuclear protein Ran (RAN), Rho GDP-dissociation inhibitor 1 (ARHGDIA), eukaryotic translation initiation factor 5A-1 (EIF5A) and Profilin-1(PFN1), and 10 up-regulated protein species named Heat shock cognate 71 kDa protein (HSPA8),10 kDa heat shock protein (HSPE1), and Cofilin-1(CFL-1) were identified. Among them, GTP-binding nuclear protein Ran (RAN) and Rho GDP-dissociation inhibitor 1 (ARHGDIA) were the most significantly changed (over tenfold). The proteasome subunit beta type-6 (PSMB6), ATP synthase ecto-α-subunit (ATP5A1), Aldehyde dehydrogenase 1 (ALDH1) and EIF5A were verified by immunoblot assays to be dramatically down-regulated. By STRING bioinformatics analysis revealing interactions and signaling networks it became apparent that the proteins changed they are primarily involved in transcription and proteolysis. Conclusion: Periplocin inhibited growth of lung cancer by down-regulating proteins, such as ATP5A1, EIF5A, ALDH1 and PSMB6. These findings may improve our understanding of the molecular mechanisms underlying the anti-cancer effects of periplocin on lung cancer cells. © 2014 S. Karger AG, Basel.

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