Natural Resource Development Institute of Pharmaceutics

Taipei, Taiwan

Natural Resource Development Institute of Pharmaceutics

Taipei, Taiwan
SEARCH FILTERS
Time filter
Source Type

Korinek M.,Kaohsiung Medical University | Tsai Y.-H.,Kaohsiung Medical University | El-Shazly M.,Kaohsiung Medical University | El-Shazly M.,Ain Shams University | And 16 more authors.
Frontiers in Pharmacology | Year: 2017

Increasing prevalence of allergic diseases with an inadequate variety of treatment drives forward search for new alternative drugs. Fatty acids, abundant in nature, are regarded as important bioactive compounds and powerful nutrients playing an important role in lipid homeostasis and inflammation. Phytochemical study on Typhonium blumei Nicolson and Sivadasan (Araceae), a folk anti-cancer and anti-inflammatory medicine, yielded four oxygenated fatty acids, 12R-hydroxyoctadec-9Z,13E-dienoic acid methyl ester (1) and 10R-hydroxyoctadec-8E,12Z-dienoic acid methyl ester (2), 9R-hydroxy-10E-octadecenoic acid methyl ester (3), and 12R*-hydroxy-10E-octadecenoic acid methyl ester (4). Isolated compounds were identified by spectroscopic methods along with GC-MS analysis. Isolated fatty acids together with a series of saturated, unsaturated and oxygenated fatty acids were evaluated for their anti-inflammatory and anti-allergic activities in vitro. Unsaturated (including docosahexaenoic and eicosapentaenoic acids) as well as hydroxylated unsaturated fatty acids exerted strong anti-inflammatory activity in superoxide anion generation (IC50 2.14-3.73 μM) and elastase release (IC50 1.26-4.57 μM) assays. On the other hand, in the anti-allergic assays, the unsaturated fatty acids were inactive, while hydroxylated fatty acids showed promising inhibitory activity in A23187- and antigen-induced degranulation assays (e.g., 9S-hydroxy-10E,12Z-octadecadienoic acid, IC50 92.4 and 49.7 μM, respectively). According to our results, the presence of a hydroxy group in the long chain did not influence the potent anti-inflammatory activity of free unsaturated acids. Nevertheless, hydroxylation of fatty acids (or their methyl esters) seems to be a key factor for the anti-allergic activity observed in the current study. Moreover, ChemGPS-NP was explored to predict the structure-activity relationship of fatty acids. The anti-allergic fatty acids formed different cluster distant from clinically used drugs. The bioactivity of T. blumei, which is historically utilized in folk medicine, might be related to the content of fatty acids and their metabolites. © 2017 Korinek, Tsai, El-Shazly, Lai, Backlund, Wu, Lai, Wu, Chen, Wu, Cheng, Hwang, Chen and Chang.


Chung C.-H.,National Taiwan University of Science and Technology | Yeh S.-C.,Natural Resource Development Institute of Pharmaceutics | Tseng H.-C.,Joben Bio medical CompanyPingtung County | Siu M.-L.,Natural Resource Development Institute of Pharmaceutics | Lee K.-T.,National Taiwan University of Science and Technology
Journal of Food and Drug Analysis | Year: 2016

The fruiting body of Antrodia cinnamomea is used as a medicinal mushroom in Taiwan and is found on the inner cavity of the endemic species Cinnamomum kanehirai. In this study, phytomics similarity index (PSI) analysis was employed for the chemical quality evaluation of the A. cinnamomea fruiting bodies from different strains, and grown on various substrates. The results indicated that the different types of A. cinnamomea fruiting bodies contain eight index compounds, and that it was difficult to discriminate between them solely on the basis of those index compounds. In our research, we used PSI scores to assess the metabolite similarity of the fruiting bodies of A. cinnamomea. It was revealed that fruiting bodies from various A. cinnamomea strains grown on different culture substrates produce distinct PSI scores. We concluded that PSI analysis had good selectivity on the different types of A. cinnamomea fruiting bodies. © 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.


Chung C.-H.,National Taiwan University of Science and Technology | Yeh S.-C.,Natural Resource Development Institute of Pharmaceutics | Tseng H.-C.,Joben Bio medical Company | Siu M.-L.,Natural Resource Development Institute of Pharmaceutics | Lee K.-T.,National Taiwan University of Science and Technology
Journal of Food and Drug Analysis | Year: 2015

The fruiting body of Antrodia cinnamomea is used as a medicinal mushroom in Taiwan and is found on the inner cavity of the endemic species Cinnamomum kanehirai. In this study, phytomics similarity index (PSI) analysis was employed for the chemical quality evaluation of the A. cinnamomea fruiting bodies from different strains, and grown on various substrates. The results indicated that the different types of A. cinnamomea fruiting bodies contain eight index compounds, and that it was difficult to discriminate between them solely on the basis of those index compounds. In our research, we used PSI scores to assess the metabolite similarity of the fruiting bodies of A. cinnamomea. It was revealed that fruiting bodies from various A. cinnamomea strains grown on different culture substrates produce distinct PSI scores. We concluded that PSI analysis had good selectivity on the different types of A. cinnamomea fruiting bodies. © 2015.

Loading Natural Resource Development Institute of Pharmaceutics collaborators
Loading Natural Resource Development Institute of Pharmaceutics collaborators