Natural Product Research Laboratory

Amritsar, India

Natural Product Research Laboratory

Amritsar, India
SEARCH FILTERS
Time filter
Source Type

Bandau F.,Umeå University | Albrectsen B.R.,Umeå University | Julkunen-Tiitto R.,Natural Product Research Laboratory | Gundale M.J.,Swedish University of Agricultural Sciences
Plant and Soil | Year: 2016

Background and aims: Boreal forests can receive substantial nitrogen (N) enrichment via atmospheric N deposition and industrial forest fertilization. While it is known that N enrichment can impact ecosystem properties, such as litter decomposition, it remains poorly understood how genetic variability within plant species modifies these impacts. Methods: We grew replicates of ten Populus tremula L. genotypes (GTs) under 3 N conditions; ambient, and levels representing atmospheric N deposition and industrial forest fertilization. We measured leaf and litter physical and chemical traits, and conducted a litter decomposition assay. Results: Leaf traits varied due to N treatment, GT, and constitutive tannin levels. Leaf traits were in some cases correlated with litter traits, and decomposition was influenced by single and interactive effects of N and GT. Nitrogen addition unexpectedly decelerated decomposition, potentially due to changes in specific leaf area (SLA). Variation in decomposition rates among the GTs was best explained by their differences in SLA, and lignin:N ratio. Nitrogen addition also caused a shift in which traits most strongly influenced decomposition. Conclusions: Our findings highlight that the considerable diversity present in tree species can have a strong influence on ecosystem processes, such as decomposition, and how these processes respond to environmental change. © 2016 The Author(s)


Mukhtar H.M.,Natural Product Research Laboratory | Kaur R.,Natural Product Research Laboratory | Ali B.,Natural Product Research Laboratory | Vashishth D.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2012

Zingiber officinale is an important medicinal plant belonging to family zingiberaceae. The rhizome of plant is widely used in Chinese, Ayurvedic and Unani herbal medicines all over the world for the treatment of dyspepsia, vomiting, diarrhoea, rheumatism, migraine headache, muscular disorder and dementia. Systematic Pharmacognostical evaluation of dried powdered rhizome of plant of both regions was carried out with respect to macroscopy, microscopy, physicochemical parameters and fluorescent analysis. In powder microscopy parenchymatous cells with idioblast, parenchyma with oleo resin, flattened starch grain, thickened vessels and fibres were observed. Size of microscopic characters was also measured. Physicochemical evaluation showed results like ash value- HP region [total ash (8.25%), acid insoluble ash (0.66), water soluble ash (6%) sulphated ash (2.5%)] and Assam region [total ash (8.40%), acid insoluble ash (0.65), Water soluble ash (5.66%) sulphated ash (2.3%)]. Swelling index - HP region (6.26 ml), Assam region (3.83 ml). LOD-H.P region (11.3%), Assam region (5.26%). Foaming index of both HP and Assam region was found to be less than 100. Cold maceration- HP region [ pet ether (3%), benzene (3%), ethyl acetate (3.6%), chloroform (6%), acetone (7%), methanol (23%)] Assam region [pet ether (1.6%), benzene (3.3%), ethyl acetate (4%), chloroform (6.3%), acetone (7.3%), methanol (11.65). Successive extraction- HP region [pet ether (4%), benzene (4.5%), ethyl acetate (4.98%), chloroform (6.68%), acetone (7.4%), methanol (13.2%)] Assam region [pet ether (3%), benzene (3.5%), ethyl acetate (4.6%) chloroform (7%), acetone (7.2%), methanol (10.4)]. The results provided useful data that is essential for standardisation and characterisation of powdered drug. © RJPT All right reserved.


Mukhtar H.M.,Natural Product Research Laboratory | Mir P.A.,Natural Product Research Laboratory | Ali B.,Natural Product Research Laboratory | Yadav S.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2012

Portulaca oleracea is commonly known as purslane belonging to family Portulaceae. It has been used as folk medicine in many countries as diuretic, febrifuge, antiseptic, antimicrobial effects. Systematic Pharmacognostical evaluation of dried powdered plant of purslane has been carried out according to WHO guidelines with respect to macroscopy, microscopy, physicochemical parameters, chemical reactions and fluorescent analysis etc. Physicochemical evaluation results like total ash 15.25%, water solube ash 6.33%, acid insoluble ash 3.5%, foreign matter 2.23%, moisture content 3.45% respectively. Tannin content was found to be 3.02%. Both successive and cold maceration in various solvents like petroleum ether (3.3%), benzene (6.3%), chloroform (9.3%), ethyl acetate (11.6%), methanol (17.6%). Cold maceration values, petroleum ether (3.3%), benzene (4.2%), chloroform (8.0%), ethyl acetate (11.0%), methanol (17.1%). Behaviour on treatment with different chemical reagents of the dried whole plant, fluorescence characters under both visible and ultralviolet light (short uv 254nm and long uv 360nm) was compared with Asian nerolac synthetic and puja satin enamel colour reference card. Further preliminary phytochemical screening of different extracts of whole herb revealed the presence of alkaloids, glycosides, steroids, flavonoids, phenols, proteins and amino acids. © RJPT All right reserved.


Mukhtar H.M.,Natural Product Research Laboratory | Yadav S.,Natural Product Research Laboratory | Ali B.,Natural Product Research Laboratory | Mir P.A.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2012

The standardization parameters like macroscopical, microscopical characters, physiochemical parameters like total ash (4.66%), water soluble ash (3.66%), acid insoluble ash (0.65%), sulphated ash (0.50%), foreign organic matter, loss on drying, foaming index (≥100), both successive and cold maceration in various solvents like petroleum ether (1.66%), benzene (1.00%), chloroform (1.33%), ethyl acetate (1.66%), methanol (6.00%). Cold maceration values, petroleum ether (1.00%), benzene (1.60%), chloroform (2.00%), ethyl acetate (2.30%), methanol (13.30%). Behaviour on treatment with different chemical reagents of the dried root, fluorescence characters under both visible and ultralviolet light (254 nm, 366 nm). Further preliminary phytochemical screening of different extracts of root revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, fixed oil, tannin and phenols, proteins and amino acids. © RJPT.


Mukhtar H.M.,Natural Product Research Laboratory | Wadhan P.,Natural Product Research Laboratory | Singh V.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2011

In present study, various standardization parameters like macroscopical, microscopical characters, physiochemical parameters like total ash (14.3%), water soluble ash (5.1%), acid insoluble ash (3.7%), sulphated ash (2.3%), foreign organic matter, loss on drying, swelling index (5.1%), both successive and cold maceration in various solvents like petroleum ether (3.5%), benzene (4.2%), chloroform (6.0%), ethyl acetate (6.5%), methanol (13%). Cold maceration values, petroleum ether (3.0%), benzene (3.5%), chloroform (5.0%), ethyl acetate (5.6%), methanol (11.8%). Behaviour on treatment with different chemical reagents of the dried flower heads, fluorescence characters under both visible and ultralviolet light was compared with nerolac synthetic and satin enamel colour reference card. Further preliminary phytochemical screening of different extracts of flower heads revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, phenols, proteins and amino acids. © RJPT All right reserved.


Jain A.,Natural Product Research Laboratory | Singhai A.K.,Natural Product Research Laboratory
Natural Product Research | Year: 2010

The ethanolic extract of the fruits of Momordica dioica was studied for its protective and curative effect against gentamicin-induced acute renal injury in albino rats of both sexes. Gentamicin intoxicated group showed significant increase in blood urea (69.48 ± 4.34) and serum creatinine (3.017± 0.208) from normal levels 33.72 ±1.92 and 0.818± 0.073, respectively, in control group. In the preventive regimen, the extract at dose levels of 200 mg kg-1 showed significant reduction in the elevated blood urea (47.93± 2.46) and serum creatinine (2.067± 0.1745), respectively. This treatment normalised the histopathological changes compared to the intoxicated group. In the curative regimen at 200 mg kg-1 blood urea was found to be 48.21± 2.36 and serum creatinine level was 2.050± 0.183, which revealed significant curative effect. In vivo antioxidant and free radial scavenging activities were also determined. The maximum free radical scavenging activity with ethanolic extract was the basis of selection of this extract for in vivo study. Reduced glutathione (GSH) level was significantly (p 0.05) increased in the extract treated groups whereas malondialdehyde (MDA) was reduced significantly (p < 0.05). High content of flavonoids and phenolic compounds was found in ethanolic extract, which may be responsible for free radical activity. The findings suggest that the ethanol extract of Momordica dioica seeds possesses marked nephroprotective and curative activities without any toxicity due to its antioxidant activity and could offer a promising role in the treatment of acute renal injury caused by nephrotoxin-like gentamicin. © 2010 Taylor & Francis.


Jain A.,Natural Product Research Laboratory | Singhai A.K.,Natural Product Research Laboratory
Natural Product Research | Year: 2010

In this study, the ethanol extract of Momordica dioica fruit extract (200 mg kg-1) was studied for nephroprotective and curative activities. Chloroform, ethyl acetate, ethanol and aqueous extracts were prepared. In vitro antioxidant activity was made the basis for the selection of the ethanol extract for further studies. In DPPH free radical scavenging activity, the ethanolic extract has shown maximum inhibition (84.2%), followed by aqueous (74.8%), ethyl acetate (69.4%) and chloroform (59.7%) extract. On the other hand, in total antioxidant activity, the ethanol extract has shown 80.1% inhibition, followed by aqueous (71.9%), ethyl acetate (67.2%) and chloroform (53.2%) extracts. A single dose (5 mg kg-1, i.p.) of cisplatin was administrated to induce nephrotoxicity. Blood urea and serum creatinine were analysed as biochemical markers of nephrotoxicity. Reduced glutathione (GSH) and the product of lipid peroxidation (MDA) were also measured in kidney tissues. A single dose of cisplatin resulted in significant reduction in body weight and increased the urea and creatinine levels. Extract administration has shown significant recovery in the levels of these biochemicals in curative (p<0.001) and protective groups, whereas a single dose of cisplatin caused significant reduction in GSH and an increase in malondialdehyde production. Recovery was observed in treated groups. This study suggested that the nephroprotective and curative activities of M. dioica fruit extract are due to its antioxidant activity. It is further concluded that this antioxidant activity may be attributed to the phenolics, flavonoids and amino acids present in the extract. © 2010 Taylor & Francis.


Yasir M.,Natural Product Research Laboratory | Yasir M.,Siksha ‘O’ Anusandhan University | Jain P.,Natural Product Research Laboratory | Debajyoti,Siksha ‘O’ Anusandhan University | Kharya M.D.,Natural Product Research Laboratory
International Journal of Phytomedicine | Year: 2010

Acacia arabica commonly known as babool are used in traditional Indian medicine for treatment of diabetes mellitus. The hypoglycemic effect of aqueous extract (hot and cold water) and hydroalcoholic extract of Acacia arabica was investigated. Oral administration of cold water extract of Acacia arabica bark to diabetic and normal rats at a dose of 400 mg/kg body weight resulted in significant reduction of blood glucose, cholesterol and triglycerides. Phytochemical investigations found that phenolic compounds are presents in Acacia arabica extracts. The cold water extract of Acacia arabica was found to reduce blood glucose level to its normal level with in seven days. Histological studies of the β-cells show its action on pancreas. © arjournals.org, All rights reserved.


Mukhtar H.M.,Natural Product Research Laboratory | Vashishth D.,Natural Product Research Laboratory | Ali B.,Natural Product Research Laboratory | Kaur R.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2012

This specification covers the standardization and preliminary phytochemical investigation of the plant Benincasa hispida (Cucurbitaceae). It is widely used as antiulcer, anti-angiogenic, bronchodilator, in opioid withdrawal benefit, anti-diabetic and in mental disability. The plant material was subjected to pharmacognostical studies like macroscopic and microscopic studies (powder microscopy, micrometry, histochemical tests and transverse section). The microscopic characters revealed the presence of: Seeds [lignified trichomes found in groups (17.28μ,1.62μ), transparent fibers (70.20μ, 1.35μ), round shaped oil cells (9.45μ) found in thin walled parenchyma cells, parenchyma cells with wrinkled walls and lignified spiral vessels (52.38μ, 1.48μ)] mesocarp [lignified covering trichomes (27.43μ, 1.73μ) fibers (34.10μ, 2.22μ), lignified parenchyma cells (14.58) and vascular bundles]. Dried plant material was subjected to various physiochemical parameters like Hot extraction- seeds [water (15%) alcohol (7%)] mesocarp [water (60%) alcohol (25%)], ash values {seeds [total ash value (4%), water soluble ash (1%), acid insoluble ash (0.5%) sulphated ash value (3%)] mesocarp [total ash value (10%), water soluble ash (8%), acid insoluble ash (0.5%) sulphated ash value (7%)]}, cold maceration- {[petroleum ether [seeds (29%) mesocarp (1%)], benzene [seeds (28%) mesocarp (2%)], ethyl acetate [seeds (28%) mesocarp (2%)], chloroform [seeds (28%) mesocarp (2%)], acetone [seeds (30%) mesocarp (4%)], methanol [seeds (12%) mesocarp (31%)], water [seeds (11%) mesocarp (44%)]}, TLC with different solvents and at different wavelengths, fluorescent analysis, loss on drying, foaming index, swelling index, microscopic studies (powder microscopy, micrometry, transverse section, histo-chemical tests), successive extraction {[petroleum ether [seeds (18%) mesocarp (1.2%)], benzene [seeds (1.2%) mesocarp (1.2%)], ethyl acetate [seeds (1.8%) mesocarp (3.6%)], chloroform [seeds (1.6%) mesocarp (5.6%)], acetone [seeds (2.4%) mesocarp (8.8%)], methanol[seeds (4%) mesocarp (15.2%)]. The preliminary phytochemical investigation revealed the presence of amino acids, triterpenoids, tannins, carbohydrates. These parameters can be utilized for quick identification of the Benincasa hispida and are particularly useful in powdered form. © RJPT.


PubMed | Natural Product Research Laboratory
Type: Journal Article | Journal: Natural product research | Year: 2010

The ethanolic extract of the fruits of Momordica dioica was studied for its protective and curative effect against gentamicin-induced acute renal injury in albino rats of both sexes. Gentamicin intoxicated group showed significant increase in blood urea (69.48 +/- 4.34) and serum creatinine (3.017 +/- 0.208) from normal levels 33.72 +/- 1.92 and 0.818 +/- 0.073, respectively, in control group. In the preventive regimen, the extract at dose levels of 200 mg kg(-1) showed significant reduction in the elevated blood urea (47.93 +/- 2.46) and serum creatinine (2.067 +/- 0.1745), respectively. This treatment normalised the histopathological changes compared to the intoxicated group. In the curative regimen at 200 mg kg(-1) blood urea was found to be 48.21 +/- 2.36 and serum creatinine level was 2.050 +/- 0.183, which revealed significant curative effect. In vivo antioxidant and free radial scavenging activities were also determined. The maximum free radical scavenging activity with ethanolic extract was the basis of selection of this extract for in vivo study. Reduced glutathione (GSH) level was significantly (p < 0.05) increased in the extract treated groups whereas malondialdehyde (MDA) was reduced significantly (p < 0.05). High content of flavonoids and phenolic compounds was found in ethanolic extract, which may be responsible for free radical activity. The findings suggest that the ethanol extract of Momordica dioica seeds possesses marked nephroprotective and curative activities without any toxicity due to its antioxidant activity and could offer a promising role in the treatment of acute renal injury caused by nephrotoxin-like gentamicin.

Loading Natural Product Research Laboratory collaborators
Loading Natural Product Research Laboratory collaborators