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Jain A.,Natural Product Research Laboratory | Singhai A.K.,Natural Product Research Laboratory
Natural Product Research | Year: 2010

The ethanolic extract of the fruits of Momordica dioica was studied for its protective and curative effect against gentamicin-induced acute renal injury in albino rats of both sexes. Gentamicin intoxicated group showed significant increase in blood urea (69.48 ± 4.34) and serum creatinine (3.017± 0.208) from normal levels 33.72 ±1.92 and 0.818± 0.073, respectively, in control group. In the preventive regimen, the extract at dose levels of 200 mg kg-1 showed significant reduction in the elevated blood urea (47.93± 2.46) and serum creatinine (2.067± 0.1745), respectively. This treatment normalised the histopathological changes compared to the intoxicated group. In the curative regimen at 200 mg kg-1 blood urea was found to be 48.21± 2.36 and serum creatinine level was 2.050± 0.183, which revealed significant curative effect. In vivo antioxidant and free radial scavenging activities were also determined. The maximum free radical scavenging activity with ethanolic extract was the basis of selection of this extract for in vivo study. Reduced glutathione (GSH) level was significantly (p 0.05) increased in the extract treated groups whereas malondialdehyde (MDA) was reduced significantly (p < 0.05). High content of flavonoids and phenolic compounds was found in ethanolic extract, which may be responsible for free radical activity. The findings suggest that the ethanol extract of Momordica dioica seeds possesses marked nephroprotective and curative activities without any toxicity due to its antioxidant activity and could offer a promising role in the treatment of acute renal injury caused by nephrotoxin-like gentamicin. © 2010 Taylor & Francis. Source

Jain A.,Natural Product Research Laboratory | Singhai A.K.,Natural Product Research Laboratory
Natural Product Research | Year: 2010

In this study, the ethanol extract of Momordica dioica fruit extract (200 mg kg-1) was studied for nephroprotective and curative activities. Chloroform, ethyl acetate, ethanol and aqueous extracts were prepared. In vitro antioxidant activity was made the basis for the selection of the ethanol extract for further studies. In DPPH free radical scavenging activity, the ethanolic extract has shown maximum inhibition (84.2%), followed by aqueous (74.8%), ethyl acetate (69.4%) and chloroform (59.7%) extract. On the other hand, in total antioxidant activity, the ethanol extract has shown 80.1% inhibition, followed by aqueous (71.9%), ethyl acetate (67.2%) and chloroform (53.2%) extracts. A single dose (5 mg kg-1, i.p.) of cisplatin was administrated to induce nephrotoxicity. Blood urea and serum creatinine were analysed as biochemical markers of nephrotoxicity. Reduced glutathione (GSH) and the product of lipid peroxidation (MDA) were also measured in kidney tissues. A single dose of cisplatin resulted in significant reduction in body weight and increased the urea and creatinine levels. Extract administration has shown significant recovery in the levels of these biochemicals in curative (p<0.001) and protective groups, whereas a single dose of cisplatin caused significant reduction in GSH and an increase in malondialdehyde production. Recovery was observed in treated groups. This study suggested that the nephroprotective and curative activities of M. dioica fruit extract are due to its antioxidant activity. It is further concluded that this antioxidant activity may be attributed to the phenolics, flavonoids and amino acids present in the extract. © 2010 Taylor & Francis. Source

Gupta A.K.,Natural Product Research Laboratory | Kaur K.,Natural Product Research Laboratory | Alam N.,Jazan University | Ahmad S.,Jamia Hamdard University | And 2 more authors.
Research Journal of Pharmacy and Technology | Year: 2012

Boerhaavia diffusa Linn., belonging to family Nyctaginaceae, commonly known as "Punarnava", is a perennial creeping herb, up to a height of 1-1.2 m. Parts of the plant have been used since long time in the treatment of various diseases. The roots have been reported to possess activities like diuretic, anti-inflammatory, laxative anthelmintic, stomachic, febrifuge, antileprosy, antiscabies, antiviral, and antitumour properties. In the present study, comparative standardization of the root of B. diffusa from two different geographical regions (Punjab and U.P) have been studied as per the WHO Guidelines to determine the correct identity and purity of the plant part and for the detection of adulteration as well. Botanical authentication and physicochemical parameters gave an idea about the quality of drug. The root powder was extracted with different solvents including Petroleum ether, Benzene, Chloroform, Ethyl acetate, Methanol and Water. Pharmacognostic study (macroscopic and powder microscopic) and physicochemical parameters (extractive values, ash values, foaming index, foreign matter, moisture content) were studied. The extractive values of hot extraction with methanol was found 15.3% w/w and 14.8%w/w and aqueous hot extractive was found 19.3%w/w and 19.7%w/w in Punjab and U.P. regions respectively. Total ash values were found 8.5%w/w and 9.15%w/w in Punjab and U.P. regions respectively. Further phytochemical screening revealed the presence of alkaloids, flavonoids, triterpenoids, phenolic compounds (tannins), saponins and steroids. © RJPT All right reserved. Source

Mukhtar H.M.,Natural Product Research Laboratory | Wadhan P.,Natural Product Research Laboratory | Singh V.,Natural Product Research Laboratory
Research Journal of Pharmacy and Technology | Year: 2011

In present study, various standardization parameters like macroscopical, microscopical characters, physiochemical parameters like total ash (14.3%), water soluble ash (5.1%), acid insoluble ash (3.7%), sulphated ash (2.3%), foreign organic matter, loss on drying, swelling index (5.1%), both successive and cold maceration in various solvents like petroleum ether (3.5%), benzene (4.2%), chloroform (6.0%), ethyl acetate (6.5%), methanol (13%). Cold maceration values, petroleum ether (3.0%), benzene (3.5%), chloroform (5.0%), ethyl acetate (5.6%), methanol (11.8%). Behaviour on treatment with different chemical reagents of the dried flower heads, fluorescence characters under both visible and ultralviolet light was compared with nerolac synthetic and satin enamel colour reference card. Further preliminary phytochemical screening of different extracts of flower heads revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, phenols, proteins and amino acids. © RJPT All right reserved. Source

Yasir M.,Natural Product Research Laboratory | Yasir M.,Siksha O' Anusandhan University | Jain P.,Natural Product Research Laboratory | Debajyoti,Siksha O' Anusandhan University | Kharya M.D.,Natural Product Research Laboratory
International Journal of Phytomedicine | Year: 2010

Acacia arabica commonly known as babool are used in traditional Indian medicine for treatment of diabetes mellitus. The hypoglycemic effect of aqueous extract (hot and cold water) and hydroalcoholic extract of Acacia arabica was investigated. Oral administration of cold water extract of Acacia arabica bark to diabetic and normal rats at a dose of 400 mg/kg body weight resulted in significant reduction of blood glucose, cholesterol and triglycerides. Phytochemical investigations found that phenolic compounds are presents in Acacia arabica extracts. The cold water extract of Acacia arabica was found to reduce blood glucose level to its normal level with in seven days. Histological studies of the β-cells show its action on pancreas. © arjournals.org, All rights reserved. Source

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