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Debre Zeyit, Ethiopia

Silva A.C.,Institute Biologia Experimental e Tecnologica IBET | Silva A.C.,University of Lisbon | Yami M.,National Veterinary Institute NVI | Libeau G.,CIRAD - Agricultural Research for Development | And 4 more authors.
Vaccine | Year: 2014

In this paper extended tests on a new candidate formulation for Peste des Petits Ruminants (PPR) vaccine carried out at National Veterinary Institute (NVI) in Ethiopia are presented. This work was performed in the frame of the VACNADA project from GALVmed which aimed at procuring vaccines against neglected veterinary diseases to African vaccine producing laboratories, in particular PPR.After the eradication of Rinderpest, Peste des Petits Ruminants became the next veterinary disease on target for elimination, requiring an effective and thermostable vaccine. In this work a Tris/Trehalose formulation was evaluated in thermal stability studies in comparison to the current used formulation of the live attenuated PPR vaccine, the Weybridge medium. The extended results presented herein show an increased thermal stability of the vaccine, especially at 37 and 45. °C, as expected from previously published results (Silva A.C. et al., 2011). Furthermore, during the course of this project, the NVI teams have clearly demonstrated ability to produce higher quality PPR vaccines after a successful transfer of the technology. These results should significantly enhance the utility of the vaccine in the eradication of PPR. © 2014 Elsevier Ltd. Source

Ashenafi G.,National Veterinary Institute NVI | Roger F.,CIRAD - Agricultural Research for Development | Waret-Szkuta A.,CIRAD - Agricultural Research for Development | Waret-Szkuta A.,Royal Veterinary College
PLoS ONE | Year: 2012

Proximity and affiliation to the local market appear to be two of the most relevant factors to explain farmer's choices to select a particular trading point. Physical barriers may limit the options, especially in developing countries. A network of villages linked by traders/farmer-traders sharing livestock markets was built with field data collected in 75 villages from 8 kebelles in the Wassona Werna wereda of the Ethiopian Highlands. Two exponential random graph models were fitted with various geographical and demographic attributes of the nodes (dyadic independent model) and three internal network structures (dyadic dependent model). Several diagnostic methods were applied to assess the goodness of fit of the models. The odds of an edge where the distance to the main market Debre Behran and the difference in altitude between two connected villages are both large increases significantly so that villages far away from the main market and at different altitude are more likely to be linked in the network than randomly. The odds of forming an edge between two villages in Abamote or Gudoberet kebelles are approximately 75% lower than an edge between villages in any other kebelles (p<0.05). The conditional log-odds of two villages forming a tie that is not included in a triangle, a 2-star or a 3-star is extremely low, increasing the odds significantly (p<0.05) each time a node is in a 2-star structure and decreasing it when a node is in a 3-star (p<0.05) or in a triangle formation (p<0.05)), conditional on the rest of the network. Two major constraining factors, namely distance and altitude, are not deterrent for the potential contact of susceptible small ruminant populations in the Highlands of Ethiopia. © 2012 Ortiz-Pelaez et al. Source

Gari G.,National Animal Health Diagnostic and Investigation Center | Abie G.,National Animal Health Diagnostic and Investigation Center | Gizaw D.,National Animal Health Diagnostic and Investigation Center | Wubete A.,National Animal Health Diagnostic and Investigation Center | And 7 more authors.
Vaccine | Year: 2015

The safety, immunogenicity and efficacy of three commercially available vaccines against lumpy skin disease (LSD) in cattle have been evaluated using a combination of vaccine challenge experiments and the monitoring of immune responses in vaccinated animals in the field. The three vaccines evaluated in the study included two locally produced (Ethiopian) vaccines (lumpy skin disease virus (LSDV) Neethling and Kenyan sheep and goat pox (KSGP) O-180 strain vaccines) and a Gorgan goat pox (GTP) vaccine manufactured by Jordan Bio-Industries Centre (JOVAC). The latter vaccine was evaluated for the first time in cattle against LSDV. The Ethiopian Neethling and KSGPO-180 vaccines failed to provide protection in cattle against LSDV, whereas the Gorgan GTP vaccine protected all the vaccinated calves from clinical signs of LSD. There was no significant difference in protective efficacy detected between two dosage levels (P= 0.2, P= 0.25, and P= 0.1 for KSGP, Neethling and Gorgan vaccines, respectively). Additionally, the Gorgan GTP vaccinated cattle showed stronger levels of cellular immune responses measured using Delayed-Type Hypersensitivity (DTH) reactions at the vaccination site indicating higher levels of immunogenicity produced by the GTPV vaccine in cattle, as opposed to the other two vaccines. This study indicated, for the first time, that the Gorgan GTP vaccine can effectively protect cattle against LSDV and that the Neethling and KSGP O-180 vaccine were not protective. The results emphasise the need for molecular characterization of the Neethling and KSGP O-180 vaccine seed viruses used for vaccine production in Ethiopia. In addition, the potency and efficacy testing process of the Ethiopian LSD Neethling and KSGP O-180 vaccines should be re-evaluated. © 2015 Elsevier Ltd. Source

Sanden M.,National Institute of Nutrition And Seafood Research | Johannessen L.E.,National Veterinary Institute NVI | Berdal K.G.,National Veterinary Institute NVI | Sissener N.,National Institute of Nutrition And Seafood Research | Hemre G.-I.,National Institute of Nutrition And Seafood Research
Aquaculture Nutrition | Year: 2011

The aim of this study was to examine the presence or absence of dietary transgenic (Roundup Ready ® soybean - RRS ®) and soybean DNA (sRubisco) in the intestinal tract of Atlantic salmon fed either genetically modified (GM) or conventional (non-GM) soybeans. Uptake of dietary DNA was evaluated in the post gastric intestine (pyloric ceca - PC, mid intestine - MI and distal intestine - DI) after continuous feeding (6months), feed restriction and re-feeding using qPCR and in situ hybridization. No transgenic DNA fragments were detected in any of the intestinal samples using event specific primers. Soybean DNA was detected in all segments of the intestinal tissue (PC, MI and DI) and visualized in the cell vacuolar system of the DI in the apex area of the intestinal fold. Dietary DNA was gradually cleared from the intestinal tissues when feed was restricted and could not be detected after 5days. Re-feeding resulted in dietary plant DNA uptake after 2h. The results show that the salmon intestine is able to take up dietary plant DNA shortly after feed intake and that one of the factors affecting uptake and clearance of nucleic acids in the various intestinal segments are the feeding status of the fish. © 2011 Blackwell Publishing Ltd. Source

Bayissa B.,Addis Ababa Institute of Technology | Ayelet G.,National Veterinary Institute NVI | Kyule M.,Addis Ababa Institute of Technology | Jibril Y.,Addis Ababa Institute of Technology | Gelaye E.,National Veterinary Institute NVI
Tropical Animal Health and Production | Year: 2011

Cross-sectional serological study and questionnaire survey were conducted in Borana pastoral and agro-pastoral area to determine seroprevalence and risk factors associated with foot-and-mouth disease (FMD) infection and to assess community perceptions as to importance of the disease. A multistage random sampling was carried out to select cattle for seroprevalence and households for interviews. Totally, 768 sera were collected from 111 herds. The overall individual level seroprevalence of 23.0% (n=177) and herd level seroprevalence of 58.6% (n=65) were recorded using 3ABC ELISA test. The variation of individual level seroprevalence in districts were statistically significant (P < 0.05) which was 29.9% in Arero, 24.0% in Yabello, and 15.7% in Teltele. From multivariate logistic regression analysis, herd size and age were seen to be significantly (P < 0.05) associated with FMD seroprevalence. The result of the questionnaire survey based on 120 respondents indicated that, the daily milk yield of cows infected with FMD during outbreaks is reduced to an average of 0.5 L for 25.5 days while cows developing heat-intolerance syndrome after acute infection gave an average 0.67 L for 3.8 months and their calving interval prolonged about 12 months. The questionnaire survey in agro-pastoral area of Borena also indicated that FMD-infected oxen remained off-plough for one season when outbreaks occur in cropping time, whereas heat-intolerant oxen were no longer used for traction. These findings of the present study indicated that FMD is a highly prevalent and economically important disease in the Borana pastoral and agro-pastoral production systems which need effective control strategy for the disease. © 2011 Springer Science+Business Media B.V. Source

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