National University of Tainan and Bio Jourdeness International Group Co. | Date: 2016-12-23
A method of manufacturing methoxy-isoflavones by biotransformation and a use thereof are revealed herein. The method comprises the steps of synthesizing a nucleic acid sequence including a SpOMT2884 gene ofStreptomyces peucetius; cloning the nucleic acid sequence into an expression vector to form a cyclic recombinant plasmid; transforming the cyclic recombinant plasmid into a microbial expression system (Escherichia coli); and incubating the microbial expression system in a medium containing 8-hydroxydaidzeins therein for generating methoxy-isoflavones.
Lo Y.-L.,National University of Tainan |
Wang W.,National University of Tainan
Chemico-Biological Interactions | Year: 2013
The frequent development of multidrug resistance (MDR) hampers the efficacy of available anticancer drugs in treating cervical cancer. In this study, we aimed to use formononetin (7-hydroxy-4′-methoxyisoflavone), a potential herbal isoflavone, to intensify the chemosensitivity of human cervical cancer HeLa cells to epirubicin, an anticancer drug. The reactive oxygen species (ROS) levels were correlated with MDR modulation mechanisms, including the transporter inhibition and apoptosis induction. Our results revealed that formononetin significantly enhanced the cytotoxicity of epirubicin. Co-incubation of epirubicin with formononetin increased the ROS levels, including hydrogen peroxide and superoxide free radicals. Epirubicin alone markedly increased the mRNA expression of MDR1, MDR-associated protein (MRP) 1, and MRP2. In contrast, formononetin alone or combined treatment decreased the mRNA expression of MRP1 and MRP2. This result indicates that efflux transporter-mediated epirubicin resistance is inhibited at different degrees by the addition of formononetin. This isoflavone significantly intensified epirubicin uptake into HeLa cells. Apoptosis was induced by formononetin and/or epirubicin, as signified by nuclear DNA fragmentation, chromatin condensation, increased sub-G1 and G2/M phases. The cotreatment triggered the mitochondrial apoptotic pathway indicated by increased Bax-to-Bcl-2 expression ratio, loss of mitochondrial membrane potential, and significant activation of caspase-9 and -3. In addition, extrinsic/caspases-8 apoptotic pathway was also induced by the cotreatment. N-acetyl cysteine abrogated these events induced by formononetin, supporting the involvement of ROS in the MDR reversal mechanism. This study pioneered in demonstrating that formononetin may potentiate the cytotoxicity of epirubicin in HeLa cells through the ROS-mediated MRP inhibition and concurrent activation of the mitochondrial and death receptor pathways of apoptosis. Hence, the circumvention of pump and non-pump resistance using formononetin and epirubicin may pave the way for a powerful chemotherapeutic regimen for treating human cervical cancer.© 2013 Elsevier Ireland Ltd. All rights reserved.
Bio Jourdeness International Group Co. and National University of Tainan | Date: 2016-05-11
A composite comprising 3-hydroxygenistein and a use for inhibition of melanogenesis are revealed herein. An effective dose of 3-hydroxygenistein is applied to skin for inhibition of tyrosinase activity and further inhibition of melanogensis, so that 3-hydroxygenistein can be used in cosmetic composition.
Yeh C.-T.,National University of Tainan
Fuzzy Sets and Systems | Year: 2011
Recently, many scholars investigated interval, triangular, and trapezoidal approximations of fuzzy numbers. These publications can be grouped into two classes: Euclidean distance class and non-Euclidean distance class. Most approximations in Euclidean distance class can be calculated by formulas, but calculating approximations in the other class is more complicated. In the present paper, we study a special class of non-linear approximations with respect to a weighted Euclidean distance. We call it "weighted semi-trapezoidal approximations". The proposed approximations generalize all recent approximations in Euclidean distance class. First, we embed fuzzy numbers into a Hilbert space. Then compute weighted semi-trapezoidal approximations by means of best approximations in a closed convex subset of the Hilbert space. Finally, we propose formulas of matrix type, which is more clear than the previous contributions. © 2010 Elsevier B.V. All rights reserved.
Hwang J.-J.,National University of Tainan
Journal of Power Sources | Year: 2013
The objective of the present paper is to conduct an experimental work to measure transient thermal/electrical efficiencies in a combined heat and power (CHP) fuel cell generator. An original-designed fuel cell CHP generator comprising a proton exchange membrane (PEM) fuel cell system, a power conditioning system, and a thermal recovery system is constructed. The thermal recovery system recaptures the thermal energy dissipated by the fuel cell stack, while the power conditioning system distributes the electrical power of the fuel cell system. Performance matrices including stack electrical efficiency, system electrical efficiency, thermal efficiency, and CHP efficiency are presented. Particular attention is placed on the variation of transient system efficiencies as the load is changed drastically. The time-averaged system efficiency is then concluded from the statistics of the transient system efficiency. Results show that the time-averaged efficiencies in electrical, thermal and CHP are about 37%, 24%, and 61%, respectively, under 55% electrical power capacity of the generator. The instantaneous CHP efficiency could reach as high as 85% in the loading period. © 2012 Elsevier B.V. All rights reserved.
Hsu C.-L.,National University of Tainan |
Chang S.-J.,National Cheng Kung University
Small | Year: 2014
In the past decades, the doping of ZnO one-dimensional nanostructures has attracted a great deal of attention due to the variety of possible morphologies, large surface-to-volume ratios, simple and low cost processing, and excellent physical properties for fabricating highperformance electronic, magnetic, and optoelectronic devices. This article mainly concentrates on recent advances regarding the doping of ZnO one-dimensional nanostructures, including a brief overview of the vapor phase transport method and hydrothermal method, as well as the fabrication process for photodetectors. The dopant elements include B, Al, Ga, In, N, P, As, Sb, Ag, Cu, Ti, Na, K, Li, La, C, F, Cl, H, Mg, Mn, S, and Sn. The various dopants which act as acceptors or donors to realize either p-type or n-type are discussed. Doping to alter optical properties is also considered. Lastly, the perspectives and future research outlook of doped ZnO nanostructures are summarized. © 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Hwang J.J.,National University of Tainan
Renewable and Sustainable Energy Reviews | Year: 2012
The current development of fuel cell scooters has been reviewed in this paper. Fuel cell scooters, by nature, have zero emissions, and they have the potential to replace current petroleum-propelled engine scooters. First, the fundamentals of fuel cells, including the critical technologies pertaining to fuel cell engines and hydrogen storage, were introduced. Then, the technical feasibility of fuel cell scooters was discussed in parallel with the hydrogen infrastructure model. The accomplishments of fuel cell scooters in Taiwan were presented. Moreover, the contribution of replacing petrol scooters by hydrogen fuel cell scooters to reduction in greenhouse gas (GHG) emission and energy conservation was evaluated. Furthermore, industrial competition with regard to the development of fuel cell scooters was discussed on the basis of a strengths, weaknesses, opportunities, and threats (SWOT) analysis. In conclusion, with mature fuel cell technology together with solid foundation of the scooter industry, Taiwan offers conditions that were conducive for the development of fuel cell scooters. Its social and technical capability will be proved on account of the leading demonstrations of fuel cell scooters in the world. If it can develop a successful business model, Taiwan could enjoy the advantages of tapping the huge global market for zero-emission scooters. © 2012 Elsevier Ltd. All right reserved.
Hwang J.J.,National University of Tainan
Renewable and Sustainable Energy Reviews | Year: 2010
On June 12, 2009, Taiwan government approved a bill of "Statute for Renewable Energy Development", which aimed at promoting the use of renewable energy, boosting energy diversification, and helping reduce greenhouse gases. The new act caps the subsidies for renewable energy up to 10 GW within 20 years. It authorizes the government to enhance incentives for the development of renewable energy via a variety of methods, including the acquisition mechanisms, incentives for demonstration projects, and the loosening of regulatory restrictions. According to the subsidies of the Statute, the share of power capacity of renewable energies will be triple of the nationwide power installation capacity by 2029. The purpose of this paper is to present an updated overview of promotional policy of renewable energy in Taiwan, in addition to evaluate the growth space of individual renewable energies in the post-Statute era by considering the technology development, domestic conditions, and indigenous industries related to renewable energy. © 2009 Elsevier Ltd. All rights reserved.
Tseng D.-Y.,National University of Tainan
Mitochondrial DNA | Year: 2013
In this study, the complete mitogenome sequence of Onychostoma barbata has been determined using long polymerase chain reaction method. The mitogenome, consisting of 16,592 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR). CR of 937 bp lengths long is located between tRNAPro and tRNAPhe. The overall base composition of O. barbata is 24.49% for T, 28.04% for C, 31.54% for A, and 15.94% for G, with a slight AT bias of 56.03%. © 2012 Informa UK, Ltd.
National University of Tainan | Date: 2014-10-01
A passive anode gas recovery system for fuel cells is revealed. The system includes a fuel cell, a fuel supply device, an electronically controlled regulator, a first ejection module, a second ejection module, a hydrogen recovery module, and a controller. The system is a passive fuel recovery system disposed on an outlet end of an anode of the fuel cell. By the controller, the hydrogen recovery module recovers unconsumed hydrogen gas in the fuel cell provided by the fuel supply device into two ejection modules with different orifice diameters for recycling and reuse. The system has advantages of low cost, no extra energy consumed, and no external controller required. The system can be applied to developing fuel cell systems with high efficiency and low cost.