The National University of Singapore is a comprehensive research university located in Singapore, being the flagship tertiary institution of the country which has a global approach to education and research. Founded in 1905, it is the oldest higher learning institute in Singapore, as well as the largest university in the country in terms of student enrolment and curriculum offered. It was ranked as the best university in Asia by QS University Rankings in 2014.The university's main campus is located in southwest Singapore at Kent Ridge, with an area of approximately 1.83 km2 . The Bukit Timah campus houses the Faculty of Law, Lee Kuan Yew School of Public Policy and research institutes, while the Duke-NUS Graduate Medical School Singapore is located at the Outram campus. Wikipedia.
Brigham, Women's Hospital, Dana-Farber Cancer Institute and National University of Singapore | Date: 2017-02-01
Described herein are methods and compositions which lead to the efficient ex vivo expansion of hematopoietic cells, such as hematopoietic stem cells (HSCs) and hematopoietic stem and progenitor cells HSPCs. Using combinations of small molecule drugs and cytokines/growth factors/grown factors targeting epigenetic status in cells, significant improvements in the expansion of cells was observed, including cells isolated from human cord blood or peripheral mobilized stem/progenitor cells. Multiple genes implicated in HSPC function were unperturbed, and efficiency of genomic editing using lentivirus was greatly enhanced following treatment. These novel approaches could be used therapeutically in a variety of hematopoietic transplantation settings, in addition to benefiting gene therapy techniques.
National University of Singapore | Date: 2017-05-10
Characterizing, identifying, or diagnosing the type and/or nature of a sample or a tissue such as an abnormal growth using a Raman spectrum includes analyzing distinct spectral subintervals within the Raman spectrum in two distinct wavelength ranges, such as FP and HW wavelength ranges, to identify a match with one or more reference markers in one or both wavelength ranges; and from the match characterizing, identifying, or diagnosing the type and/or nature of the sample or tissue. FP and HW Raman spectra can be detected or acquired simultaneously using a single diffraction grating.
National University of Singapore and Singapore Health Services Pte Ltd | Date: 2017-01-18
The present invention provides methods and agents for modulating WARS2 expression, WARS2 activity, or a combination thereof, thereby modulating angiogenesis. Also provided herein are methods for identifying individuals who could benefit from agents that modulate WARS2 expression, WARS2 activity, or a combination thereof.
National University of Singapore | Date: 2017-03-08
A disposable measurement tip for an instrument for measuring at least one parameter of a sample, the measurement tip comprising; a tip body; a filling channel in the tip body for holding the sample to be analyzed and having an opening on one end of the tip body to receive the sample; a population of micro-particles held within the tip body filling channel and which can be mixed with the sample when the sample is received in the filling channel; wherein in use the sample in the tip is illuminated by the instrument and the illumination which impinges on the micro-particles is directed back, by the micro-particles, to the instrument to be detected and to thereby enable the at least one parameter to be determined.
National University of Singapore and St Jude Childrens Research Hospital | Date: 2017-03-22
The present invention provides, in certain aspects, a natural killer (NK) cell that expresses all or a functional portion of interleukin-15 (IL-15), and methods for producing such cells. The invention further provides methods of using a natural killer (NK) cell that expresses all or a functional portion of interleukin-15 (IL-15) to treat cancer in a subject or to enhance expansion and/or survival of NK cells.
National University of Singapore | Date: 2017-01-18
An optical interference device 100 is disclosed herein. In a described embodiment, the optical interference device 100 comprises a phase shifter array 108 for receiving a collimated beam of light. The phase shifter array 108 includes an array of cells 128 for producing optical light channels from respective rays of the collimated beam of light, with at least some of the optical light channels having varying phase shifts. The optical interference device 100 further includes a focusing lens 110 having a focal distance and arranged to simultaneously produce, from the optical light channels, a focused beam of light in its focal plane and an image downstream the phase shifter array 108 for detection by an optical detector 116. The optical interference device 100 also includes an optical spatial filter 112 arranged at the focal distance of the focusing lens 110 and arranged to filter the focused beam of light to produce a spatially distributed interference light pattern in zeroth order for detection by the optical detector 116. A method for producing a spatially distributed interference light pattern is also disclosed.
National University of Singapore and Agency For Science | Date: 2017-03-15
The present invention relates, in various embodiments, to methods of producing a tissue-mimetic construct having a basement membrane, methods of producing an acellular scaffold containing an extracellular matrix (ECM), methods of producing a scaffold comprising a hydrogel that is enriched in ECM components, methods of treating a condition in a subject in need thereof with a tissue-mimetic construct having a basement membrane, and methods of assessing whether an agent is suitable for administering to a tissue. The invention further relates to tissue-mimetic constructs and scaffolds produced in accordance with the methods of the invention.
Iridex Corporation, National University Hospital Singapore and National University of Singapore | Date: 2017-05-03
Systems, devices, and methods for treating a glaucomatous eye are provided. Embodiments may provide a treatment probe for treating an eye of a patient. The treatment probe may have an elongate body with a contact surface at a distal end of the elongate body. A treatment fiber or light source may be housed in the treatment probe and may be configured to direct treatment energy from the contact surface. The contact surface may be configured to couple to a surface of the eye to deliver the energy into the target area. In many embodiments the contact surface may have a convex configuration with a rounded outer shape and edge that facilitates the sweeping of the probe surface across the eye during treatment delivery. In some embodiments the probe may be swept in arc motions while delivering treatment energy to the eye.
Reid D.W.,National University of Singapore
Nature Reviews Neuroscience | Year: 2015
Pioneering electron microscopy studies defined two primary populations of ribosomes in eukaryotic cells: one freely dispersed through the cytoplasm and the other bound to the surface of the endoplasmic reticulum (ER). Subsequent investigations revealed a specialized function for each population, with secretory and integral membrane protein-encoding mRNAs translated on ER-bound ribosomes, and cytosolic protein synthesis was widely attributed to free ribosomes. Recent findings have challenged this view, and transcriptome-scale studies of mRNA distribution and translation have now demonstrated that ER-bound ribosomes also function in the translation of a large fraction of mRNAs that encode cytosolic proteins. These studies suggest a far more expansive role for the ER in transcriptome expression, where membrane and secretory protein synthesis represents one element of a multifaceted and dynamic contribution to post-transcriptional gene expression. © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
Woodberry R.D.,National University of Singapore
American Political Science Review | Year: 2012
This article demonstrates historically and statistically that conversionary Protestants (CPs) heavily influenced the rise and spread of stable democracy around the world. It argues that CPs were a crucial catalyst initiating the development and spread of religious liberty, mass education, mass printing, newspapers, voluntary organizations, and colonial reforms, thereby creating the conditions that made stable democracy more likely. Statistically, the historic prevalence of Protestant missionaries explains about half the variation in democracy in Africa, Asia, Latin America and Oceania and removes the impact of most variables that dominate current statistical research about democracy. The association between Protestant missions and democracy is consistent in different continents and subsamples, and it is robust to more than 50 controls and to instrumental variable analyses. © 2012 American Political Science Association.